HORTELÃ-PIMENTA

Effect of the ultrasound-assisted preliminary maceration on the efficiency of the essential oil distillation from selected herbal raw materials.

Autores: Radosław Kowalski, Grażyna Kowalska, Jerzy Jamroz, Agnieszka Nawrocka, Damian Metyk | Ano: 2015 | Database: pubmed

Resumo: The objective of the study was the estimation of the ultrasound-assisted preliminary maceration effect on the efficiency of essential oil distillation and on its qualitative and quantitative composition. The experiment included analyses on three herbal materials, i.e. peppermint leaves (Mentha piperita L.), marjoram herb (Origanum majorana L.), and chamomile flowers (Chamomilla recutita (L.) Rauschert). The study showed that the application of preliminary water maceration of raw material, augmented with ultrasounds, had a statistically significant increasing effect on the amount of oil distilled, in the case of mint leaf from 1.32% to 1.46% v/w, and in the case of marjoram herb from 1.13% to 1.27% v/w. In the case of chamomile flowers no significant effect of ultrasound on the amount of oil obtained was observed. Generally, comparing the composition of essential oils obtained in the experiments with the requirements of the relevant standards no unfavourable effect of the distillation augmenting techniques applied was noted. Therefore, it should be expected that the studied distillates of volatile fractions will have the desired biological activity.

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Conclusão: N/A

Toxicity of Essential Oil of Mentha piperita (Lamiaceae) and its Monoterpenoid Menthol Against Tetranychus urticae Kogan 1836 (Acari: Tetranychidae).

Autores: Lauana P DE Souza, Vitor Zuim, Priscila Stinguel, Patrícia F Pinheiro, Hugo B Zago | Ano: 2022 | Database: pubmed

Resumo: Essential oils from plants have remarkable biological properties, for example as insecticides and acaricides. Here we provide chemical analysis and evaluate the toxicity of the essential oil of Mentha piperita (Lamiaceae) and its main constituent menthol against Tetranychus urticae Kogan 1836 (Acari: Tetranychidae), a polyphagous pest present in agricultural landscapes. The essential oil was obtained from M. piperita leaves via hydrodistillation. Subsequently, concentration-response bioassays in adult females (fumigation and contact) were conducted to evaluate the lethal effect on the mite with three exposure intervals. We also evaluated the reproductive performance of females after exposure. Both substances were lethal in the fumigation bioassay, in addition, the essential oil was about 6-fold more toxic than menthol after 24 and 48 h of exposure. The fecundity of T. urticae females decreased inversely proportional to the increase of the used concentrations. Essential oil contact tests showed sublethal effects, with low mortality and reproductive stimulation of T. urticae females. Therefore, menthol and M. piperita essential oil can be considered potential acaricides for T. urticae by fumigant exposure due to the deleterious effect in adults and reduction in the number of individuals in subsequent generations, that represents a promising management tool.

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Conclusão: N/A

Toxicity of Essential Oil of Mentha piperita (Lamiaceae) and its Monoterpenoid Menthol Against Tetranychus urticae Kogan 1836 (Acari: Tetranychidae).

Autores: Lauana P DE Souza, Vitor Zuim, Priscila Stinguel, Patrícia F Pinheiro, Hugo B Zago | Ano: 2022 | Database: pubmed

Resumo: Essential oils from plants have remarkable biological properties, for example as insecticides and acaricides. Here we provide chemical analysis and evaluate the toxicity of the essential oil of Mentha piperita (Lamiaceae) and its main constituent menthol against Tetranychus urticae Kogan 1836 (Acari: Tetranychidae), a polyphagous pest present in agricultural landscapes. The essential oil was obtained from M. piperita leaves via hydrodistillation. Subsequently, concentration-response bioassays in adult females (fumigation and contact) were conducted to evaluate the lethal effect on the mite with three exposure intervals. We also evaluated the reproductive performance of females after exposure. Both substances were lethal in the fumigation bioassay, in addition, the essential oil was about 6-fold more toxic than menthol after 24 and 48 h of exposure. The fecundity of T. urticae females decreased inversely proportional to the increase of the used concentrations. Essential oil contact tests showed sublethal effects, with low mortality and reproductive stimulation of T. urticae females. Therefore, menthol and M. piperita essential oil can be considered potential acaricides for T. urticae by fumigant exposure due to the deleterious effect in adults and reduction in the number of individuals in subsequent generations, that represents a promising management tool.

Resultados: N/A

Conclusão: N/A

Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model.

Autores: Mohammad Modarresi, Mohammad-Reza Farahpour, Behzad Baradaran | Ano: 2019 | Database: pubmed

Resumo: This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25  107 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.

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Conclusão: N/A

Peppermint Essential Oil and Its Major Volatiles as Protective Agents against Soft Rot Caused by Fusarium sambucinum in Cera Pepper (Capsicum pubescens).

Autores: Marco Antonio Kevin Pérez-Vázquez, Yesenia Pacheco-Hernández, Edmundo Lozoya-Gloria, Clemente Mosso-González, Sergio A Ramírez-García, Omar Romero-Arenas, Nemesio Villa-Ruano | Ano: 2022 | Database: pubmed

Resumo: Cera pepper (Capsicum pubescens) is an exotic fruit considered as a rich source of nutraceuticals with known benefits for human health and also an economic resource for local producers in Mexico. The present investigation reports on the in vitro and in situ antifungal activity of the essential oil from Mentha piperita and its two major volatiles (menthol and menthone) against Fusarium sambucinum, which is a causal agent of soft rot in cera pepper. The application of these components in pepper fruits previously infected with F. sambucinum caused a significant delay (p<0.05) in the emergence of soft rot symptoms. This effect was reflected in the maintenance of pH and fruit firmness during a period of 10 days. The nutrimental content of the fruits (protein, fiber, fat and other proximate parameters) was conserved in the same period of time. The nutraceutical content of these fruits was estimated by the quantification of seven carotenoids (violaxanthin, cis-violaxanthin, luteoxanthin, antheraxanthin, lutein, zeaxanthin and β-carotene), ascorbic acid and capsaicinoids (capsaicin and dihydrocapsaicin). According to our results, the essential oil from M. Piperita and its major volatiles exerted a preservative effect on these metabolites. Our findings demonstrated that the essential oil of M. Piperita and its major volatiles represent an ecological alternative for the control of fusariosis caused by F. sambucinum in cera peppers under postharvest conditions.

Resultados: N/A

Conclusão: N/A

Peppermint Essential Oil and Its Major Volatiles as Protective Agents against Soft Rot Caused by Fusarium sambucinum in Cera Pepper (Capsicum pubescens).

Autores: Marco Antonio Kevin Pérez-Vázquez, Yesenia Pacheco-Hernández, Edmundo Lozoya-Gloria, Clemente Mosso-González, Sergio A Ramírez-García, Omar Romero-Arenas, Nemesio Villa-Ruano | Ano: 2022 | Database: pubmed

Resumo: Cera pepper (Capsicum pubescens) is an exotic fruit considered as a rich source of nutraceuticals with known benefits for human health and also an economic resource for local producers in Mexico. The present investigation reports on the in vitro and in situ antifungal activity of the essential oil from Mentha piperita and its two major volatiles (menthol and menthone) against Fusarium sambucinum, which is a causal agent of soft rot in cera pepper. The application of these components in pepper fruits previously infected with F. sambucinum caused a significant delay (p<0.05) in the emergence of soft rot symptoms. This effect was reflected in the maintenance of pH and fruit firmness during a period of 10 days. The nutrimental content of the fruits (protein, fiber, fat and other proximate parameters) was conserved in the same period of time. The nutraceutical content of these fruits was estimated by the quantification of seven carotenoids (violaxanthin, cis-violaxanthin, luteoxanthin, antheraxanthin, lutein, zeaxanthin and β-carotene), ascorbic acid and capsaicinoids (capsaicin and dihydrocapsaicin). According to our results, the essential oil from M. Piperita and its major volatiles exerted a preservative effect on these metabolites. Our findings demonstrated that the essential oil of M. Piperita and its major volatiles represent an ecological alternative for the control of fusariosis caused by F. sambucinum in cera peppers under postharvest conditions.

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Conclusão: N/A

Mentha piperita (peppermint).

Autores: Elise Herro, Sharon E Jacob | Ano: 2010 | Database: pubmed

Resumo: PEPPERMINT (Mentha piperita) is a popular herb that can be used in numerous forms (ie, oil, leaf, leaf extract, and leaf water). Peppermint oil has the most uses, and use data on the oil are considered relevant to the leaf extract formulations as well. This herbal preparation is used in cosmeceuticals, personal hygiene products, foods, and pharmaceutical products for both its flavoring and fragrance properties. Peppermint oil possesses a fresh sharp menthol odor and a pungent taste followed by a cooling sensation. It also has a variety of therapeutic properties and is used in aromatherapy, bath preparations, mouthwashes, toothpastes, and topical preparations. Topical preparations of peppermint oil have been used to calm pruritus and relieve irritation and inflammation. Their frequent application to impaired skin could contribute to the sensitization rates seen. Numerous allergic contact dermatitis reactions to peppermint oil have been described, many of which are linked to both perioral and intraoral disorders. Although peppermint oil is known for its many properties, its role as a sensitizer should be recognized so as to aid in the diagnosis of both dermatitis and oral conditions and to allow the initiation of proper avoidance measures.

Resultados: N/A

Conclusão: N/A

Mentha piperita (peppermint).

Autores: Elise Herro, Sharon E Jacob | Ano: 2010 | Database: pubmed

Resumo: PEPPERMINT (Mentha piperita) is a popular herb that can be used in numerous forms (ie, oil, leaf, leaf extract, and leaf water). Peppermint oil has the most uses, and use data on the oil are considered relevant to the leaf extract formulations as well. This herbal preparation is used in cosmeceuticals, personal hygiene products, foods, and pharmaceutical products for both its flavoring and fragrance properties. Peppermint oil possesses a fresh sharp menthol odor and a pungent taste followed by a cooling sensation. It also has a variety of therapeutic properties and is used in aromatherapy, bath preparations, mouthwashes, toothpastes, and topical preparations. Topical preparations of peppermint oil have been used to calm pruritus and relieve irritation and inflammation. Their frequent application to impaired skin could contribute to the sensitization rates seen. Numerous allergic contact dermatitis reactions to peppermint oil have been described, many of which are linked to both perioral and intraoral disorders. Although peppermint oil is known for its many properties, its role as a sensitizer should be recognized so as to aid in the diagnosis of both dermatitis and oral conditions and to allow the initiation of proper avoidance measures.

Resultados: N/A

Conclusão: N/A

Salicylic Acid and Melatonin Alleviate the Effects of Heat Stress on Essential Oil Composition and Antioxidant Enzyme Activity in Mentha piperita and Mentha arvensis L.

Autores: Milad Haydari, Viviana Maresca, Daniela Rigano, Alireza Taleei, Ali Akbar Shahnejat-Bushehri, Javad Hadian, Sergio Sorbo, Marco Guida, Caterina Manna, Marina Piscopo, Rosaria Notariale, Francesca De Ruberto, Lina Fusaro, Adriana Basile | Ano: 2019 | Database: pubmed

Resumo: The aim of this study was to evaluate changes in the chemical profile of essential oils and antioxidant enzymes activity (catalase CAT, superoxide dismutase SOD, Glutathione S-transferases GST, and Peroxidase POX) in Mentha piperita L. (Mitcham variety) and Mentha arvensis L. (var. piperascens), in response to heat stress. In addition, we used salicylic acid (SA) and melatonin (M), two brassinosteroids that play an important role in regulating physiological processes, to assess their potential to mitigate heat stress. In both species, the heat stress caused a variation in the composition of the essential oils and in the antioxidant enzymatic activity. Furthermore both Salicylic acid (SA) and melatonin (M) alleviated the effect of heat stress.

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Conclusão: N/A

Peppermint essential oil: its phytochemistry, biological activity, pharmacological effect and application.

Autores: Hui Zhao, Shan Ren, Han Yang, Shun Tang, Chenyang Guo, Maolun Liu, Qiu Tao, Tianqi Ming, Haibo Xu | Ano: 2022 | Database: pubmed

Resumo: Mentha (also known as peppermint), a genus of plants in the taxonomic family Lamiaceae (mint family), is widely distributed throughout temperate regions of the world. Mentha contains various constituents that are classified as peppermint essential oil (PEO) and non-essential components. PEO, consisting mainly of menthol, menthone, neomenthol and iso-menthone, is a mixture of volatile metabolites with anti-inflammatory, antibacterial, antiviral, scolicidal, immunomodulatory, antitumor, neuroprotective, antifatigue and antioxidant activities. Mounting evidence indicates that PEO may pharmacologically protect gastrointestinal, liver, kidney, skin, respiratory, brain and nervous systems, and exert hypoglycemic and hypolipidemic effects. Clinically, PEO is used for gastrointestinal and dermatological diseases, postoperative adjuvant therapy and other fields. This review aims to address the advances in the extraction and isolation of PEO, its biological activities, pharmacological effects, toxicity and applications, with an emphasis on the efficacy of PEO on burn wounds and psoriasis, providing a comprehensive foundation for research, development and application of PEO in future.

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Biocomposites of Silk-Elastin and Essential Oil from Mentha piperita Display Antibacterial Activity.

Autores: Diana S Gomes, André da Costa, Ana Margarida Pereira, Margarida Casal, Raul Machado | Ano: 2022 | Database: pubmed

Resumo: In this study, novel antimicrobial biocomposite films comprising a genetically engineered silk-elastin protein polymer (SELP) and essential oil from Mentha piperita (MPEO) have been fabricated and tested for the antibacterial performance. SELP/MPEO biocomposite films were prepared by solvent casting using water as the solvent and aqueous emulsions of MPEO at different concentrations. Emulsions of MPEO were investigated, showing that the mixing method, relative amount of surfactant, and the presence of SELP influence particle size and homogeneity. The aqueous emulsions of SELP/MPEO were characterized by a population of particles between 100 and 300 nm, depending on the MPEO concentration. The emulsified oil droplets at the highest concentration showed to be homogeneously distributed into the SELP matrix and demonstrated antibacterial activity against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. Moreover, the antibacterial activity of the biocomposite films was retained after a period of storage for 7 days at 4 °C. The formulation of composites comprising natural active fillers and recombinant protein polymers opens opportunities to develop new green, functional biocomposite materials, paving the way for a new generation of multifunctional materials.

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Antiemetic activity of volatile oil from Mentha spicata and Mentha piperita in chemotherapy-induced nausea and vomiting.

Autores: Z Tayarani-Najaran, E Talasaz-Firoozi, R Nasiri, N Jalali, Mk Hassanzadeh | Ano: 2013 | Database: pubmed

Resumo: This study is aimed at determining the efficacy of Mentha spicata (M. spicata) and Mentha piperita (M. piperita) in preventing chemotherapy-induced nausea and vomiting (CINV).

Resultados: There was a significant reduction in the intensity and number of emetic events in the first 24 h with M. spicata and M. piperita in both treatment groups (p < 0.05) when compared with the control and no adverse effects were reported. The cost of treatment was also reduced when essential oils were used.

Conclusão: M. spicata or M. piperita essential oils are safe and effective for antiemetic treatment in patients, as well as being cost effective.

The effect of aromatherapy with peppermint essential oil on nausea and vomiting after cardiac surgery: A randomized clinical trial.

Autores: Mahla Maghami, Mohammad Reza Afazel, Ismail Azizi-Fini, Mahboobeh Maghami | Ano: 2020 | Database: pubmed

Resumo: Background Postoperative nausea and vomiting are common in patients who underwent cardiac surgery. This study aimed to examine the effect of peppermint essential oil inhalation on the postoperative nausea and vomiting after cardiac surgery. Methods In this clinical trial study, 60 cardiac surgery patients were divided into control and intervention groups. The intervention group underwent nebulizer aromatherapy with peppermint essential oil before the endotracheal tube was removed after surgery. Patients' nausea and vomiting were then assessed through a checklist. The independent-samples t-test, chi-square, and Generalized estimating equation were used for data analysis. Results Totally 85.7% of the patients undergone coronary artery bypass graft surgery. The two groups did not significantly differ in terms of their baseline demographic and clinical variables (P > 0.05). Significant differences were found between the intervention and control groups in terms of the frequency of nausea (0.63 ± 0.81 vs. 1.46 ± 1.21), its duration (3.78 ± 5.09 vs. 7.97 ± 5.55 min), and severity (2.43 ± 2.84 vs. 4.61 ± 2.85), and in the frequency of vomiting episodes (0.17 ±.46 vs. 0.73 ±.60) in the first four hours after extubation (P < 0.05). Conclusion: Peppermint essential oil inhalation has beneficial effects on reducing nausea and vomiting after open-heart surgery. Using peppermint essential oil inhalation for managing postoperative nausea and vomiting is recommended.

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Antispasmodic effect of Mentha piperita essential oil on tracheal smooth muscle of rats.

Autores: Albertina Antonielly Sydney de Sousa, Pedro Marcos Gomes Soares, Arisa Nara Saldanha de Almeida, Alana Rufino Maia, Emmanuel Prata de Souza, Ana Maria Sampaio Assreuy | Ano: 2010 | Database: pubmed

Resumo: Mentha piperita is a plant popularly known in Brazil as "hortelã-pimenta" whose essential oil is used in folk medicine for its anti-inflammatory, antispasmodic, expectorant actions and anti-congestive. Here, it was investigated the effect of Mentha piperita essential oil (peppermint oil) in rat tracheal rings along with its mechanism of action.

Resultados: Peppermint oil [100 and 300 microg/ml] inhibited the contractions induced by carbachol, which was reversed by indomethacin, L-N-metyl-nitro-arginine and hexamethonium, but not by tetraethylammonium. These data suggest the participation of prostaglandin E(2), nitric oxide and autonomic ganglions in the peppermint oil relaxant effect and may be correlated with its popular use in respiratory diseases.

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Antifibrogenic Influence of Mentha piperita L. Essential Oil against CCl4-Induced Liver Fibrosis in Rats.

Autores: Hanan A Ogaly, Nadia A Eltablawy, Reham M Abd-Elsalam | Ano: 2018 | Database: pubmed

Resumo: Essential oils of some aromatic plants provide an effective nonmedicinal option to control liver fibrosis. Mentha piperita L. essential oil (MPEO) have been reported to possess protective effects against hepatotoxicity. However, its effect against liver fibrosis remains unknown. The present study investigated the antifibrogenic potential of MPEO and its underlying mechanisms. Forty male rats divided into 4 groups were used: group 1 served as normal control, group 2 (liver fibrosis) received CCl4 (2.5 mL/kg, IP, twice weekly) for 8 weeks, group 3 concurrently received CCl4 plus MPEO (50 mg/kg, IP, daily, from the 3rd week), and group 4 received MPEO only. MPOE significantly improved the liver injury markers, lipid peroxidation (LPO), antioxidant capacity, CYP2E1 gene expressionand liver histology. Furthermore, MPOE ameliorated liver fibrosis as evidenced by the reduced expression of desmin, α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), and SMAD3 proteins. In addition, MPOE counteracted the p53 upregulation induced by CCl4 at both mRNA and protein levels. In conclusion, MPOE could effectively attenuate hepatic fibrosis mainly through improving the redox status, suppressing p53 and subsequently modulating TGF-β1 and SMAD3 protein expression. These data promote the use of MPOE as a promising approach in antifibrotic therapy.

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Antifungal Activity of Some Essential Oils Emulsions Against Fungi Contaminating Ras Cheese.

Autores: A M Hassanin, S A Soliman, S A S Abdella, Hamdy A Shaaban | Ano: 2021 | Database: pubmed

Resumo: <b>Background and Objective:</b> Ras cheese is one of the important dairy products that are consumed in great quantities. But this cheese is vulnerable to the growth of fungi during ripening and selling until consumption. Therefore, research aimed to detect fungi contaminating Ras cheese and try to resist them. <b>Materials and Methods:</b> The effect of various concentrations (0.25, 0.5, 1, 2 and 3%) of essential oils emulsions of clove (<i>Syzygium aromaticum</i>), thyme (<i>Thymus vulgaris</i>) and peppermint (<i>Mentha piperita</i>) severally on the mycelial growth of the isolated fungi as compared to the control sample was tested <i>in vitro.</i> <b>Results:</b> The results indicated that many fungal species belonging to the genera <i>Aspergillus</i>, <i>Mucor</i>, <i>Eurotium</i> and <i>Mortierella</i> were isolated from the infected Ras cheese. Ochratoxin A was found in two samples whereas recorded the highest level in sample number 1 (2.1 μg kg¹). Aflatoxin M1 was found in few levels ranged between 0.012 and 0.360 μg kg¹in cheese samples, while aflatoxin B1 and B2 weren't detected in all samples. Clove essential oil emulsion completely inhibited the growth of all tested fungi at the concentration of 0.5%, followed by thyme essential oil emulsion which inhibited the fungal growth of all fungi at the concentration of 1%, while peppermint essential oil emulsion was less effective. <b>Conclusion:</b> The research recommends that clove and thyme essential oils emulsions can be used to resist the fungi of Ras cheese. Also, suggests that more research could be done on these essential oils emulsions to produce safe foods free of fungi.

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The Effects of Mentha piperita Essential Oil on C. albicans Growth, Transition, Biofilm Formation, and the Expression of Secreted Aspartyl Proteinases Genes.

Autores: Chahrazed Benzaid, Amine Belmadani, Ryad Djeribi, Mahmoud Rouabhia | Ano: 2019 | Database: pubmed

Resumo: The rise in resistance and changes in the spectrum of Candida infections have generated enormous interest in developing new antifungal drugs using natural molecules such as plant essential oils (EOs). Antimicrobial activity against foodborne pathogenic and spoilage microorganisms has been reported for EOs. The goal of this study was to assess the effect of Mentha piperita essential oil (EO) on C. albicans growth, transition (change from blastospore to hyphae forms), and biofilm formation as well as on the expression of certain virulent genes. We show that whole EO and its vapor attenuated the yeast's growth, compared to that in the control. The effect of the EO was comparable to that of amphotericin-B (AmB). The EO and its vapor significantly decreased the morphological changes of C. albicans, reduced biofilm formation, and disrupted mature C. albicans biofilms. The effect produced by whole EO on biofilm formation/disruption was notably comparable to that observed with AmB. Exposure of C. albicans to EO and its vapor downregulated the expression of various genes, such as secreted aspartyl proteinases (SAP 1, 2, 3, 9, 10) and hyphal wall protein 1 (HWP1). Altogether, these results provide new insight into the efficacy of Mentha piperita EO against C. albicans and suggest the potential of Mentha piperita EO for use as an antifungal therapy in multiple applications.

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Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities.

Autores: Marina D Soković, Jelena Vukojević, Petar D Marin, Dejan D Brkić, Vlatka Vajs, Leo J L D van Griensven | Ano: 2009 | Database: pubmed

Resumo: The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass spectral data with those of the Wiley/NBS Library of Mass Spectra. MIC's and MFC's of the oils and their components were determined by dilution assays. Thymol (48.9%) and p-cymene (19.0%) were the main components of T. vulgaris, while carvacrol (12.8%), a-terpinyl acetate (12.3%), cis-myrtanol (11.2%) and thymol (10.4%) were dominant in T. tosevii. Both Thymus species showed very strong antifungal activities. In M. piperita oil menthol (37.4%), menthyl acetate (17.4%) and menthone (12.7%) were the main components, whereas those of M. spicata oil were carvone (69.5%) and menthone (21.9%). Mentha sp. showed strong antifungal activities, however lower than Thymus sp. The commercial fungicide, bifonazole, used as a control, had much lower antifungal activity than the oils and components investigated. It is concluded that essential oils of Thymus and Mentha species possess great antifungal potential and could be used as natural preservatives and fungicides.

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Conclusão: N/A

Screening of some essential oils against Trichosporon species.

Autores: Veena Uniyal, Seema Saxena, R P Bhatt | Ano: 2013 | Database: pubmed

Resumo: White Piedra is a superficial mycoses characterized by nodules on the hair shaft, caused by the basidiomycetous yeast Trichosporon species. In this study 25 essential oils were extracted and screened against two Trichosporon species i.e. Trichosporon asahii and Trichosporon cutaneum. Both these fungi procured from MTCC Chandigarh were maintained on yeast malt agar plates and tubes at 25 degrees C. Two screening methods viz., agar well diffusion assay and minimum inhibitory concentration were adopted for the study. The results showed that the maximum anti-yeast activity against T. asahii and T. cutaneum was demonstrated by oil of Mentha piperita showing full inhibition of both the fungi, Melaleuca alternifolia with an inhibition zone of 45 and 40 mm, Cymbopogon winterians with inhibition zone of 45 and 45 mm and Cymbopogon flexuosus with 35 and 30 mm inhibition zones. The oil of Trachyspermum ammi exhibited 10 and 20 mm, Abelmoschus moschatus exhibited 30 and 20 mm, Salvia sclarea showed 20 and 18 mm and Jasminum officinale exhibited 25 and 15 mm inhibition zones showing moderate activity. The oil of Cyperus scariosus, Pogostemon patchouli and Rosa damascene showed no inhibition zone against both the fungi while Vetiveria zizanoides exhibited no inhibition in case of T. asahii and inhibition zone of 10 mm in case of T. cutaneum demonstrating comparatively low activity against both the fungi. These results support that the essential oils can be used to cure superficial mycoses and these oils may have significant role as pharmaceuticals and preservatives.

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Conclusão: N/A

Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities.

Autores: Marina D Soković, Jelena Vukojević, Petar D Marin, Dejan D Brkić, Vlatka Vajs, Leo J L D van Griensven | Ano: 2009 | Database: pubmed

Resumo: The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass spectral data with those of the Wiley/NBS Library of Mass Spectra. MIC's and MFC's of the oils and their components were determined by dilution assays. Thymol (48.9%) and p-cymene (19.0%) were the main components of T. vulgaris, while carvacrol (12.8%), a-terpinyl acetate (12.3%), cis-myrtanol (11.2%) and thymol (10.4%) were dominant in T. tosevii. Both Thymus species showed very strong antifungal activities. In M. piperita oil menthol (37.4%), menthyl acetate (17.4%) and menthone (12.7%) were the main components, whereas those of M. spicata oil were carvone (69.5%) and menthone (21.9%). Mentha sp. showed strong antifungal activities, however lower than Thymus sp. The commercial fungicide, bifonazole, used as a control, had much lower antifungal activity than the oils and components investigated. It is concluded that essential oils of Thymus and Mentha species possess great antifungal potential and could be used as natural preservatives and fungicides.

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Conclusão: N/A

Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom.

Autores: F Diánez, M Santos, C Parra, M J Navarro, R Blanco, F J Gea | Ano: 2018 | Database: pubmed

Resumo: The antifungal properties of 12 Eos, that is, Syzygium aromaticum, Pelargonium graveolens, Lavandula angustifolia, Cupresus sempervirens, Mentha piperita, Santolina chamaecyparissus, Citrus sinensis, Pogostemon patchouli, Thymus mastichina, Thymus vulgaris, Eucalyptus globulus and Rosmarinus officinalis, were screened. The influence of five doses of each EOs was tested against Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora parasitica, Pythium aphanidermatum, Alternaria brassicae, Cladobotryum mycophilum and Trichoderma aggressivum f.sp. europaeum using disc-diffusion method. The mycelial growth inhibition and ED50 were calculated. The chemical analysis of the EOs was analysed using gas chromatography-mass spectroscopy. A total of 58 compounds were identified in the 12 EOs. All essential oils (EOs) analysed showed antifungal activity against the test pathogens in the range of 5·32-100%. The inhibitory effect of oils showed dose-dependent activity on the tested fungus. Based on the ED50 values, clove, rose geranium, peppermint and patchouli were the most effective. This study warrants further research into the practical use of EOs for the control of important myco- and phytopathogens in intensive horticulture.

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Coatings comprising chitosan and Mentha piperita L. or Mentha villosa Huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit.

Autores: Ingrid Conceição Dantas Guerra, Priscila Dinah Lima de Oliveira, Alline Lima de Souza Pontes, Ana Sílvia Suassuna Carneiro Lúcio, Josean Fechine Tavares, José Maria Barbosa-Filho, Marta Suely Madruga, Evandro Leite de Souza | Ano: 2015 | Database: pubmed

Resumo: In this study, we evaluated the efficacy of coatings comprising shrimp chitosan (CHI) and Mentha piperita L. (MPEO) or Mentha villosa Huds (MVEO) essential oils to control mold infections caused by Aspergillus niger, Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer in cherry tomato fruits (Solanum lycopersicum L.) during storage at room temperature (25°C for 12 days) and low temperature (12°C for 24 days). The effects of the coatings on the physicochemical and sensory characteristics of cherry tomato fruits during storage were also assessed. The minimum inhibitory concentration (MIC) of CHI against all test fungi was 8 mg/mL, whereas the MIC for both MPEO and MVEO was 5 μL/mL. Combinations of CHI at 4 mg/mL and MPEO or MVEO at 2.5 or 1.25 μL/mL strongly inhibited the mycelial growth and spore germination of target fungi. The coatings comprising CHI and MPEO or CHI and MVEO at the different tested concentrations delayed the growth of decay-causing fungi in artificially contaminated tomato fruit during storage at either room temperature or low temperature. The assayed coatings preserved the quality of cherry tomato fruit during storage, in terms of physicochemical and sensory attributes. These results indicate that coatings comprising CHI and MPEO or CHI and MVEO represent promising postharvest treatments to prevent common postharvest mold infections in cherry tomato fruit during storage without affecting the quality of the fruit.

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Biological control of Citrus brown spot pathogen, "Alternaria alternata" by different essential oils.

Autores: Taycir Grati Affes, Synda Chenenaoui, Hassen Zemni, Majdi Hammami, Sarra Bachkouel, Wissem Aidi Wannes, Bouzid Nasraoui, Moufida Saidani Tounsi, Salma Lasram | Ano: 2022 | Database: pubmed

Resumo: The antifungal effects of laurel, myrtle and peppermint essential oils and their combinations were investigated in vitro on two strains of Alternaria alternata mycelial growth and in vivo on detached Citrus leaf disease incidence. Myrtle essential oil was rich in α-pinene and 1,8-cineole while peppermint essential oil in menthol and menthone. 1,8-Cineole was the main component of the essential oils from laurel, laurel + myrtle and peppermint + laurel. The combined peppermint and myrtle essential oil was characterized by the predominance of menthol and 1,8-cineole. All tested essential oils, incorporated in potato-dextrose agar, inhibited A. alternata mycelial growth and had a fungistatic effect at concentration 3 mg/mL of medium. A great synergism was detected between peppermint and laurel essential oils against the two strains of A. alternata. The combined laurel and peppermint essential oil reduced mycelial growth rates of inoculated detached leaves at concentration above 1.5 mg/mL.

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African peppermint (Mentha piperita) from Morocco: Chemical composition and antimicrobial properties of essential oil.

Autores: Chraibi Marwa, Kawtar Fikri-Benbrahim, Douae Ou-Yahia, Abdellah Farah | Ano: 2017 | Database: pubmed

Resumo: To replace and avoid synthetic chemicals toxicity, there is a growing interest in the investigation of natural products from plant origin for the discovery of active compounds with antimicrobial properties. This work was devoted to determine chemical composition and antimicrobial properties of the EO of M. piperita harvested in the garden of the National Institute of Medicinal and Aromatic Plants of Morocco. Experiments have been conducted at the Microbial Biotechnology Laboratory at the Sciences and Technology Faculty, Sidi Mohamed Ben Abdellah University, Fez, Morocco. M. piperita oil was screened for its antimicrobial activity against seven bacteria and two fungi using broth microdilution method. Chemical EO analysis was performed using CPG/MS. The EO revealed menthol (46.32%), menthofuran (13.18%), menthyl acetate (12.10%), menthone (7.42%), and 1,8-cineole (6.06%) as the main constituents. The tested EO exhibited strong inhibitory effect against all tested microorganisms with minimum inhibitory concentrations ranging from 0.062% to 0.5% (v/v), except for Pseudomonas aeruginosa that was the least sensitive and was only inhibited by concentrations as high as 0.5% (v/v). The studied EO showed an antimicrobial potential. This reinforces its use as an alternative to chemical additives that can be applied to the food and drug industry.

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A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.).

Autores: Diane L McKay, Jeffrey B Blumberg | Ano: 2006 | Database: pubmed

Resumo: Peppermint (Mentha piperita L.) is one of the most widely consumed single ingredient herbal teas, or tisanes. Peppermint tea, brewed from the plant leaves, and the essential oil of peppermint are used in traditional medicines. Evidence-based research regarding the bioactivity of this herb is reviewed. The phenolic constituents of the leaves include rosmarinic acid and several flavonoids, primarily eriocitrin, luteolin and hesperidin. The main volatile components of the essential oil are menthol and menthone. In vitro, peppermint has significant antimicrobial and antiviral activities, strong antioxidant and antitumor actions, and some antiallergenic potential. Animal model studies demonstrate a relaxation effect on gastrointestinal (GI) tissue, analgesic and anesthetic effects in the central and peripheral nervous system, immunomodulating actions and chemopreventive potential. Human studies on the GI, respiratory tract and analgesic effects of peppermint oil and its constituents have been reported. Several clinical trials examining the effects of peppermint oil on irritable bowel syndrome (IBS) symptoms have been conducted. However, human studies of peppermint leaf are limited and clinical trials of peppermint tea are absent. Adverse reactions to peppermint tea have not been reported, although caution has been urged for peppermint oil therapy in patients with GI reflux, hiatal hernia or kidney stones.

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Investigations on the shelf life of rainbow trout fillets covered by quinoa biofilms enriched with different essential oils (Nigella sativa and Mentha piperita).

Autores: Kübra Güler, Telat Yanık, Gonca Alak | Ano: 2022 | Database: pubmed

Resumo: Having no adverse effects on the consumer's health, causing zero or minimal damage to the environment, and maintaining the nutritional quality of the product are too important criteria for food packaging materials. Edible biofilm packaging techniques are successful to meet many of these features. To strengthen this claim, rainbow trout (Oncorhynchus mykiss) fillets were coated with an edible film solution (obtained from quinoa starch), which attracted a lot of attention in terms of nutritional value. The prepared biofilm solutions were applied in four different groups (control, quinoa, quinoa + black seed oil, and quinoa + mint oil) and stored in refrigerator conditions (4 ± 1 °C) for 15 days. Microbiological (total aerobic mesophilic bacteria, psychrophilic bacteria, Pseudomonas, lactic acid bacteria, and Enterobacteriaceae) and chemical analyses (TBARS, TVB-N, pH) were performed on certain days of storage. At the end of the study, it was stated that coating fish fillets with edible quinoa, which was enriched by black cumin and mint essential oils, had positive chemical and microbiological results. The highest value for pH was 7.03 ± 0.09 obtained in the control group. It was found that black seed oil has antimicrobial specifications via slowing the microorganism development and prolongs the storage time. The TVB-N value was below the consumable limit value (25 mg/100g) in the treatment groups and the TBARS value was lowest (1.62 ± 0.21 μmolMA/kg) in the black seed oil group. Consequently, it is suggested that black seed oil may be used on trout fillets to prolong storage time.

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In-vitro antimicrobial activity and synergistic/antagonistic effect of interactions between antibiotics and some spice essential oils.

Autores: Sevil Toroglu | Ano: 2011 | Database: pubmed

Resumo: Spices and herbs have been used for many years by different cultures. The aim of the present study is (1) to investigate in-vitro antimicrobial effects of different spices and herbs (5 species: Rosmarinus officinalis (Rosemary), Coriandrum sativum (coriander), Micromeria fruticosa (L.) Druce subsp. Brachycalyx P.H. Davis (White micromeria), Cumium cyminum (cumin), Mentha piperita (Peppermint) against different bacteria and fungi species, and (2) to discuss the in-vitro possible effects between the plants and antibiotics. The microorganisms used were Micrococcus luteus LA 2971, Bacillus megaterium NRS, Bacillus brevis FMC 3, Enterococcus faecalis ATCC 15753, Pseudomonas pyocyaneus DC 127, Mycobacterium smegmatis CCM 2067, Escherichia coil DM, Aeromonas hydrophila ATCC 7966, Yersinia enterocolitica AU 19, Staphylococcus aureus Cowan 1, Streptococcus faecalis DC 74 bacteria, and Saccharomyces cerevisiae WET 136, Kluvyeromyces fragilis DC 98 fungi in this study. The results indicated that essential oils of Rosmarinus officinalis, Coriandrum sativum L., Micromeria fruticosa (L.) Druce subsp. brachycalyx P.H. Davis, Cumium cyminum L., Mentha piperita L. were shown antimicrobial activity in the range of 7-60 mm 2 microl(-1) inhibition zone to the microorganisms tested, using disc diffusion method. Standard antibiotic such as Gentamicin (10 microg), Cephalothin (30 microg), Ceftriaxone (10 microg), Nystatin (10 U) discs were used for comparison with the antimicrobial activities of essential oils of these plants. In addition, antibacterial activity of essential oils of these plants was researched by effects when it was used together with these standard antibiotics in vitro. However, antibacterial activity changed also by in vitro interactions between these standard antibiotics and essential oils of these plants. Synergic, additive or antagonist effects were observed in antibacterial activity.

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Optimizing Antioxidant Activity and Phytochemical Properties of Peppermint (Mentha piperita L.) by Integrative Application of Biofertilizer and Stress-Modulating Nanoparticles under Drought Stress Conditions.

Autores: Ali Ostadi, Abdollah Javanmard, Mostafa Amani Machiani, Karim Kakaei | Ano: 2022 | Database: pubmed

Resumo: Drought stress (DS) negatively affects plant growth, productivity, and quality in semi-arid and arid regions. Nowadays, application of biofertilizers and stress-modulating nanoparticles (NPs) improves plant performance under stressful conditions. The study evaluated the impacts of arbuscular mycorrhizal fungi (Myco-Root) and TiO2 NPs on the nutrient uptake, dry yield, essential oil (EO) productivity, and EO quality of peppermint (Mentha piperita L.) under different irrigation regimes. The treatments included three irrigation regimes containing irrigation after 20% (I20, well-watered), 40% (I40, mild DS), and 60% (I60, severe DS) maximum allowable depletion (MAD) percentage of the soil's available water as well as four fertilizer sources contain no fertilization (control), Myco-Root biofertilizer, TiO2 NPs, and an integrative application of Myco-Root + TiO2 NPs. The results demonstrated that the highest (195.72 g m-2) and the lowest dry yield (78.76 g m-2) of peppermint was obtained in well-watered conditions with integrative application of Myco-Root + TiO2 NPs and severe drought stress (I60) without fertilization, respectively. The dry yield of peppermint was reduced by 27.7 and 53.4% in mild (I40) and severe drought stress (I60), respectively. The maximum EO content (1.49%) and EO yield (2.30 g m-2) was recorded in mild drought stress (I40) treated with Myco-Root + TiO2 NPs. Based on the GC-MS and GC-FID analysis, 29 constituents were identified in peppermint EO, with the major constituents being menthol (38.99-52%), menthone (12.72-20.13%), 1,8-cineole (6.55-7.84%), and neo-menthol (3.14-4.52%), respectively. The maximum content of menthol, 1,8-cineole, and neo-menthol was obtained under mild drought stress (I40) fertilized with Myco-Root + TiO2 NPs. The results indicate that the integrative application of Myco-Root + TiO2 NPs could be used as an alternative method of using chemical fertilizers in sustainable agricultural systems for improving the EO quantity and quality of peppermint grown under drought stress conditions.

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Chemical Composition and Antioxidant Properties of Essential Oils from Peppermint, Native Spearmint and Scotch Spearmint.

Autores: Zhaohai Wu, Bie Tan, Yanhong Liu, James Dunn, Patricia Martorell Guerola, Marta Tortajada, Zhijun Cao, Peng Ji | Ano: 2019 | Database: pubmed

Resumo: Natural antioxidants have drawn growing interest for use in animal feed and the food industry. In the current study, essential oils (EOs) obtained from hydrodistillation of three mentha species, including Mentha piperita (peppermint), Mentha spicata (native spearmint) and Mentha gracilis (Scotch spearmint), harvested in the Midwest region in the United States, were analyzed for their chemical composition using gas chromatography-mass spectrometry, and their antioxidant properties were assessed through chemical assays, in vitro cell culture modeling and in Caenorhabditis elegans (C. elegans). The activity of ferric iron reduction and free-radical scavenging capacity were assessed through chemical-based assays, including the reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and Trolox equivalent antioxidant capacity assay (TEAC). Subsequently, the capacity of EOs to mitigate lipid peroxidation was analyzed at various doses using fresh liver homogenates from pigs. A porcine jejunum epithelial cell line (IPEC-J2) was employed as in vitro model to study the cellular antioxidant activity of the mint EOs. Finally, the effectiveness of mint EOs to alleviate acute systemic oxidative damage were evaluated in vivo using C. elegans. Data were analyzed by the MIXED procedure of SAS. Contrast statement was performed to assess linear or quadratic effects of mint EOs given at various doses. All three EOs are mostly composed of monoterpenes and their derivatives (76-90%), but differed in the major compounds, which are menthol and menthone (50%) in peppermint EO and carvone (70%) in spearmint EOs. Three mint EOs demonstrated prominent radical scavenging and Fe3+ reducing activity in chemical-based assays. In comparison with native and Scotch spearmint EOs, peppermint EO had the lowest (p < 0.05) half maximal effective concentration (EC50) in DPPH and TEAC assays and higher efficacy in the reducing power assay. All three EOs exhibited equivalent activity in mitigation of chemical-induced lipid peroxidation in liver tissues in a dose-dependent manner (linear, p < 0.001). The maximal cellular antioxidant activity (CAA) was observed at 5 µg/mL for peppermint, and 100 µg/mL for native and Scotch spearmint EOs. The addition of 25 µg/mL of both spearmint EOs increased (p < 0.05) cellular concentrations of glutathione in H2O2-treated IPEC-J2 cells, suggesting enhanced endogenous antioxidant defense. Supplementation of 100 µg/mL of peppermint or Scotch spearmint EO significantly increased (p < 0.05) the survival rate of C. elegans in response to H2O2-induced oxidative stress. The protective effect is comparable to that of supplementation of 10 µg/mL of ascorbic acid. However native spearmint EO failed to reduce the death rate within the same supplementation dose (10-200 μg/mL).

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Peppermint essential oil: its phytochemistry, biological activity, pharmacological effect and application.

Autores: Hui Zhao, Shan Ren, Han Yang, Shun Tang, Chenyang Guo, Maolun Liu, Qiu Tao, Tianqi Ming, Haibo Xu | Ano: 2022 | Database: pubmed

Resumo: Mentha (also known as peppermint), a genus of plants in the taxonomic family Lamiaceae (mint family), is widely distributed throughout temperate regions of the world. Mentha contains various constituents that are classified as peppermint essential oil (PEO) and non-essential components. PEO, consisting mainly of menthol, menthone, neomenthol and iso-menthone, is a mixture of volatile metabolites with anti-inflammatory, antibacterial, antiviral, scolicidal, immunomodulatory, antitumor, neuroprotective, antifatigue and antioxidant activities. Mounting evidence indicates that PEO may pharmacologically protect gastrointestinal, liver, kidney, skin, respiratory, brain and nervous systems, and exert hypoglycemic and hypolipidemic effects. Clinically, PEO is used for gastrointestinal and dermatological diseases, postoperative adjuvant therapy and other fields. This review aims to address the advances in the extraction and isolation of PEO, its biological activities, pharmacological effects, toxicity and applications, with an emphasis on the efficacy of PEO on burn wounds and psoriasis, providing a comprehensive foundation for research, development and application of PEO in future.

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Optimizing Antioxidant Activity and Phytochemical Properties of Peppermint (Mentha piperita L.) by Integrative Application of Biofertilizer and Stress-Modulating Nanoparticles under Drought Stress Conditions.

Autores: Ali Ostadi, Abdollah Javanmard, Mostafa Amani Machiani, Karim Kakaei | Ano: 2022 | Database: pubmed

Resumo: Drought stress (DS) negatively affects plant growth, productivity, and quality in semi-arid and arid regions. Nowadays, application of biofertilizers and stress-modulating nanoparticles (NPs) improves plant performance under stressful conditions. The study evaluated the impacts of arbuscular mycorrhizal fungi (Myco-Root) and TiO2 NPs on the nutrient uptake, dry yield, essential oil (EO) productivity, and EO quality of peppermint (Mentha piperita L.) under different irrigation regimes. The treatments included three irrigation regimes containing irrigation after 20% (I20, well-watered), 40% (I40, mild DS), and 60% (I60, severe DS) maximum allowable depletion (MAD) percentage of the soil's available water as well as four fertilizer sources contain no fertilization (control), Myco-Root biofertilizer, TiO2 NPs, and an integrative application of Myco-Root + TiO2 NPs. The results demonstrated that the highest (195.72 g m-2) and the lowest dry yield (78.76 g m-2) of peppermint was obtained in well-watered conditions with integrative application of Myco-Root + TiO2 NPs and severe drought stress (I60) without fertilization, respectively. The dry yield of peppermint was reduced by 27.7 and 53.4% in mild (I40) and severe drought stress (I60), respectively. The maximum EO content (1.49%) and EO yield (2.30 g m-2) was recorded in mild drought stress (I40) treated with Myco-Root + TiO2 NPs. Based on the GC-MS and GC-FID analysis, 29 constituents were identified in peppermint EO, with the major constituents being menthol (38.99-52%), menthone (12.72-20.13%), 1,8-cineole (6.55-7.84%), and neo-menthol (3.14-4.52%), respectively. The maximum content of menthol, 1,8-cineole, and neo-menthol was obtained under mild drought stress (I40) fertilized with Myco-Root + TiO2 NPs. The results indicate that the integrative application of Myco-Root + TiO2 NPs could be used as an alternative method of using chemical fertilizers in sustainable agricultural systems for improving the EO quantity and quality of peppermint grown under drought stress conditions.

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Comparative study of the chemical composition and antioxidant activity of six essential oils and their components.

Autores: Seun-Ah Yang, Sang-Kyung Jeon, Eun-Jung Lee, Chang-Hyun Shim, In-Seon Lee | Ano: 2010 | Database: pubmed

Resumo: The antioxidant activities and the determined major components of six popular and commercially available herb essential oils, including lavender (Lavendular angustifolia), peppermint (Mentha piperita), rosemary (Rosmarius officinalis), lemon (Citrus limon), grapefruit (Citrus paradise), and frankincense (Boswellia carteri), were compared. The essential oils were analysed by GC-MS and their antioxidant activities were determined by testing free radical-scavenging capacity and lipid peroxidation in the linoleic acid system. The major components of the essential oils of lavender, peppermint, rosemary, lemon, grapefruit, and frankincense were linalyl acetate (28.2%), menthol (33.4%), 1,8-cineole (46.1%), limonene (64.5 and 94.2%), and p-menth-2-en-ol (34.5%), respectively. The highest DPPH radical-scavenging activity was obtained by the lavender essential oil and limonene, with RC50 values of 2.1 +/- 0.23% and 2.1 +/- 0.04%, respectively. Radical-scavenging activity against the ABTS radical was highest in peppermint essential oil (1.6 +/- 0.09). Lavender oil was most effective for inhibiting linoleic acid peroxidation after 10 days.

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Antioxidant and enzyme-inhibitory activity of peppermint extracts and essential oils obtained by conventional and emerging extraction techniques.

Autores: Branimir Pavlić, Nemanja Teslić, Gokhan Zengin, Saša Đurović, Dušan Rakić, Aleksandra Cvetanović, A K Gunes, Zoran Zeković | Ano: 2021 | Database: pubmed

Resumo: Peppermint is widely used medicinal plant with distinguished bioactive potential, therefore, the aim of present work was to develop novel peppermint extracts with high activity by application of traditional and emerging separation techniques. Conventional hydrodistillation and microwave-assisted hydrodistillation (MWHD) were applied for recovery of essential oil (EO), while organic solvent extraction using Soxhlet apparatus, microwave-assisted and ultrasound-assisted process and supercritical fluid extraction (SFE) were applied for non-selective recovery of peppermint lipophilic extracts. Extracts were characterized in terms of terpenoids profile with special emphasis on content of major compounds (mentol, menthone, isomenthol and eucalyptol). Antioxidant activity (DPPH, ABTS, CUPRAC, FRAP, chelating and phosphomolybdenum assay) and enzyme-inhibitory assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase and glucosidase inhibition) were used for screening of peppermint bioactivity. MWHD was recognized as alternative for traditional process in EO recovery, while SFE extracts were useful for green production of solvent-free peppermint extracts rich in terpenoids and other lipophilic bioactives.

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Characterization of Essential Oils Obtained from Abruzzo Autochthonous Plants: Antioxidant and Antimicrobial Activities Assessment for Food Application.

Autores: Marika Pellegrini, Antonella Ricci, Annalisa Serio, Clemencia Chaves-López, Giovanni Mazzarrino, Serena D'Amato, Claudio Lo Sterzo, Antonello Paparella | Ano: 2018 | Database: pubmed

Resumo: In the present study, the essential oils (EOs) of some officinal plants from Abruzzo territory (Italy) were evaluated for their antimicrobial and antioxidant activities and their volatile fraction chemical characterization. The EOs were extracted from Rosmarinus officinalis, Origanum vulgare, Salvia officinalis, Mentha piperita, Allium sativum, Foeniculum vulgare, Satureja montana, Thymus vulgaris and Coriandrum sativum seeds. The antimicrobial activity was screened against thirteen Gram-positive and Gram-negative strains to determine the Minimal Inhibitory Concentration (MIC). The total phenolic content (TPC) and the antioxidant capacity (AOC) were assessed by means of Folin-Ciocâlteu method, and Trolox Equivalent Antioxidant Capacity with 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (TEAC/ABTS), Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays respectively. Among the nine EOs tested, T. vulgaris, S. montana, O. vulgare and C. sativum EOs showed MIC values ranging from 0.625 to 5 μL/mL. The AOC and TPC results for these species were also interesting. The major components for these EOs were thymol for T. vulgaris (44%) and O. vulgare (40%), linalool (77%) for C. sativum, and carvacrol for S. montana (54%). The results allowed the study to establish that these EOs are good candidates for potential application as biopreservatives in foods and/or food manufacture environments.

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Risk assessment of in vitro cytotoxicity, antioxidant and antimicrobial activities of Mentha piperita L. essential oil.

Autores: W M F Silva, N P Bona, N S Pedra, K F Da Cunha, A M Fiorentini, F M Stefanello, E R Zavareze, A R G Dias | Ano: 2022 | Database: pubmed

Resumo: The objective of this study was to determine the chemical composition as well as antioxidant, antibacterial, and cytotoxic properties of the essential oil of Mentha piperita L. (peppermint). Fifteen chemical constituents were identified in the essential oil, for a total of 99.99% of the compounds. The essential oil exhibited antimicrobial activity against two Gram-positive bacteria Staphylococcus aureus and Listeria monocytogenes. The minimum inhibitory concentration (MIC) of essential oil of Mentha piperita L. for Staphylococcus aureus and Listeria monocytogenes was 1.84 μg/ml, whereas the minimum bactericidal concentration (MBC) values were 3.7 and 7.43 μg/ml, respectively. The oil displayed potent antioxidant activity inhibiting up to approximately73% of 2,2'-azinothiobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. In the cytotoxicity assay, the highest essential oil concentration (100 μg/ml) resulted in viability of approximately 90% human epidermal keratinocyte (HaCaT) cells. With respect to antitumor activity in C6 rat glioma cells, there was significant reduction in cell viability: 56-74% in 24 hr, and 71-77% in 48 hr. Data suggest that in presence of the essential oil of Mentha piperita L. antioxidant, antibacterial, antitumor and non-cytotoxic properties were noted.

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Ethnomedicinal, phytochemical and pharmacological updates on Peppermint (Mentha piperita L.)-A review.

Autores: Ganesan Mahendran, Laiq-Ur Rahman | Ano: 2020 | Database: pubmed

Resumo: Peppermint (Mentha piperita L) is a perennial, glabrous and strongly scented herb belongs to the family Lamiaceae. It is cultivated in a temperate region of Europe, Asia, United States, India and Mediterranean countries due to their commercial value and distinct aroma. In addition to traditional food flavouring uses, M. piperita is well recognized for their traditional use to treat fever, cold, digestive, anti-viral, anti-fungal and oral mucosa and throat inflammation. The scientific studies provide awareness on the use of M. piperita for biological effects such as anti-oxidant, anti-microbial, anti-viral, anti-inflammatory, biopesticidal, larvicidal, anticancer, radioprotective effect, genotoxicity and anti-diabetic activity have been ascribed. A wide spectrum of bioactive phytochemicals such as flavonoids, phenolics lignans and stilbenes and essential oils are expected to be responsible for the aroma effects. In this sense, this present review provides an extensive overview of the traditional medicinal, phytochemical and multiple biological activities of this "Peppermint."

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Direct enantiomeric analysis of Mentha essential oils.

Autores: Carmen Barba, Guillermo Santa-María, Marta Herraiz, Rosa M Martínez | Ano: 2013 | Database: pubmed

Resumo: A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds.

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Direct enantiomeric analysis of Mentha essential oils.

Autores: Carmen Barba, Guillermo Santa-María, Marta Herraiz, Rosa M Martínez | Ano: 2013 | Database: pubmed

Resumo: A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds.

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Antimicrobial synergism and antibiofilm activities of Pelargonium graveolens, Rosemary officinalis, and Mentha piperita essential oils against extreme drug-resistant Acinetobacter baumannii clinical isolates.

Autores: Ayse Humeyra Taskin Kafa, Rukiye Aslan, Cem Celik, Mursit Hasbek | Ano: 2022 | Database: pubmed

Resumo: Rosemary officinalis L., Pelargonium graveolens L., and Mentha piperita L., essential oils are used by complementary medicine specialists simultaneously with traditional antibiotics for treatment purposes. The chemical composition of essential oils was analyzed by the gas chromatography-mass spectrometry method. In vitro antibacterial and antibiofilm activities of the essential oils were tested against extreme drug-resistant (XDR) colistin-resistant and colistin susceptible Acinetobacter baumannii clinical strains. The synergistic activities between essential oils and colistin antibiotics were investigated by the checkerboard method. The highest antibacterial effect was detected in mint essential oil (2.5-5 μl/ml), followed by pelargonium essential oil (5-20 μl/ml) and rosemary essential oil (5-20 μl/ml). The combination of rosemary essential oil or pelargonium essential oil with colistin showed strong synergistic activity in most of the bacterial strains tested (fractional inhibitory concentration index ≤ 0.5; synergy). As a result of the combination of mint essential oil and colistin, an indifferent effect was observed in only two bacterial strains, and other strains could not be evaluated. No antagonistic effects were observed in any of the tested essential oils. As a result of the effectiveness of the combination, the minimum inhibitory concentration (MIC) values of colistin in XDR-A. baumannii clinical isolates decreased 2-32 fold. Additionally, the sub-MIC concentration of essential oils exhibited an inhibitory effect (48-90%) against the biofilm layer of tested A. baumannii strains.

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Conclusão: N/A

Antifungal Activity of Essential Oils Against Candida Species Isolated from Clinical Samples.

Autores: S Córdoba, W Vivot, W Szusz, G Albo | Ano: 2019 | Database: pubmed

Resumo: We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l-1 for essential oils and antifungal drugs, respectively. MIC50 and MIC90, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l-1), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l-1) and intermediate for C. glabrata (MIC≤ 32 mg l-1). The essential oils showed antifungal activity on Candida spp. tested with MIC90 values ranging from 0.8 to 800 mg l-1. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC90 0.8-0.16 mg l-1), and the least active was C. officinalis (MIC90 400-800 mg l-1). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.

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Conclusão: N/A

Chemical Composition and Antimicrobial Properties of Mentha piperita cv. 'Kristinka' Essential Oil.

Autores: Ippolito Camele, Daniela Gruľová, Hazem S Elshafie | Ano: 2021 | Database: pubmed

Resumo: Several economically important crops, fruits and vegetables are susceptible to infection by pathogenic fungi and/or bacteria postharvest or in field. Recently, plant essential oils (EOs) extracted from different medicinal and officinal plants have had promising antimicrobial effects against phytopathogens. In the present study, the potential microbicide activity of Mentha piperita cv. 'Kristinka' (peppermint) EO and its main constituents have been evaluated against some common phytopathogens. In addition, the cell membrane permeability of the tested fungi and the minimum fungicidal concentrations were measured. The antifungal activity was tested against the following postharvest fungi: Botrytis cinerea, Monilinia fructicola, Penicillium expansum and Aspergillus niger, whereas antibacterial activity was evaluated against Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas savastanoi and P. syringae pv. phaseolicola. The chemical analysis has been carried out using GC-MS and the main components were identified as menthol (70.08%) and menthone (14.49%) followed by limonene (4.32%), menthyl acetate (3.76%) and β-caryophyllene (2.96%). The results show that the tested EO has promising antifungal activity against all tested fungi, whereas they demonstrated only a moderate antibacterial effect against some of the tested bacteria.

Resultados: N/A

Conclusão: N/A

Chemical Composition and Antimicrobial Properties of Mentha piperita cv. 'Kristinka' Essential Oil.

Autores: Ippolito Camele, Daniela Gruľová, Hazem S Elshafie | Ano: 2021 | Database: pubmed

Resumo: Several economically important crops, fruits and vegetables are susceptible to infection by pathogenic fungi and/or bacteria postharvest or in field. Recently, plant essential oils (EOs) extracted from different medicinal and officinal plants have had promising antimicrobial effects against phytopathogens. In the present study, the potential microbicide activity of Mentha piperita cv. 'Kristinka' (peppermint) EO and its main constituents have been evaluated against some common phytopathogens. In addition, the cell membrane permeability of the tested fungi and the minimum fungicidal concentrations were measured. The antifungal activity was tested against the following postharvest fungi: Botrytis cinerea, Monilinia fructicola, Penicillium expansum and Aspergillus niger, whereas antibacterial activity was evaluated against Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas savastanoi and P. syringae pv. phaseolicola. The chemical analysis has been carried out using GC-MS and the main components were identified as menthol (70.08%) and menthone (14.49%) followed by limonene (4.32%), menthyl acetate (3.76%) and β-caryophyllene (2.96%). The results show that the tested EO has promising antifungal activity against all tested fungi, whereas they demonstrated only a moderate antibacterial effect against some of the tested bacteria.

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Conclusão: N/A

Anti-Candida activity of Brazilian medicinal plants.

Autores: Marta Cristina Teixeira Duarte, Glyn Mara Figueira, Adilson Sartoratto, Vera Lúcia Garcia Rehder, Camila Delarmelina | Ano: 2005 | Database: pubmed

Resumo: Essential oils and ethanolic extracts from the leaves and/or roots of 35 medicinal plants commonly used in Brazil were screened for anti-Candida albicans activity. The oils were obtained by water-distillation using a Clevenger-type system. Essential oils from 13 plants showed anti-Candida activity, including Aloysia triphylla, Anthemis nobilis, Cymbopogon martini, Cymbopogon winterianus, Cyperus articulatus, Cyperus rotundus, Lippia alba, Mentha arvensis, Mikania glomerata, Mentha piperita, Mentha sp., Stachys byzantina, and Solidago chilensis. The ethanol extract was not effective at any of the concentrations tested. Chemical analyses showed the presence of compounds with known antimicrobial activity, including 1,8-cineole, geranial, germacrene-D, limonene, linalool, and menthol.

Resultados: N/A

Conclusão: N/A

Anti-Candida activity of Brazilian medicinal plants.

Autores: Marta Cristina Teixeira Duarte, Glyn Mara Figueira, Adilson Sartoratto, Vera Lúcia Garcia Rehder, Camila Delarmelina | Ano: 2005 | Database: pubmed

Resumo: Essential oils and ethanolic extracts from the leaves and/or roots of 35 medicinal plants commonly used in Brazil were screened for anti-Candida albicans activity. The oils were obtained by water-distillation using a Clevenger-type system. Essential oils from 13 plants showed anti-Candida activity, including Aloysia triphylla, Anthemis nobilis, Cymbopogon martini, Cymbopogon winterianus, Cyperus articulatus, Cyperus rotundus, Lippia alba, Mentha arvensis, Mikania glomerata, Mentha piperita, Mentha sp., Stachys byzantina, and Solidago chilensis. The ethanol extract was not effective at any of the concentrations tested. Chemical analyses showed the presence of compounds with known antimicrobial activity, including 1,8-cineole, geranial, germacrene-D, limonene, linalool, and menthol.

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Conclusão: N/A

Comparison of the antibacterial activity of essential oils and extracts of medicinal and culinary herbs to investigate potential new treatments for irritable bowel syndrome.

Autores: Aiysha Thompson, Dilruba Meah, Nadia Ahmed, Rebecca Conniff-Jenkins, Emma Chileshe, Chris O Phillips, Tim C Claypole, Dan W Forman, Paula E Row | Ano: 2013 | Database: pubmed

Resumo: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder, which may result from alteration of the gastrointestinal microbiota following gastrointestinal infection, or with intestinal dysbiosis or small intestinal bacterial overgrowth. This may be treated with antibiotics, but there is concern that widespread antibiotic use might lead to antibiotic resistance. Some herbal medicines have been shown to be beneficial, but their mechanism(s) of action remain incompletely understood. To try to understand whether antibacterial properties might be involved in the efficacy of these herbal medicines, and to investigate potential new treatments for IBS, we have conducted a preliminary study in vitro to compare the antibacterial activity of the essential oils of culinary and medicinal herbs against the bacterium, Esherichia coli.

Resultados: Most of the oils exhibited antibacterial activity in all three assays, however peppermint, lemon balm and coriander seed oils were most potent, with peppermint and coriander seed oils being more potent than the antibiotic rifaximin in the disc diffusion assay. The compounds present in these oils were identified by gas chromatography mass spectrometry. Finally, extracts were made of spearmint, lemon balm and coriander leaves with various solvents and these were tested for their antibacterial activity against E. coli in the disc diffusion assay. In each case, extracts made with ethanol and methanol exhibited potent antibacterial activity.

Conclusão: N/A

Biocomposites of Silk-Elastin and Essential Oil from Mentha piperita Display Antibacterial Activity.

Autores: Diana S Gomes, André da Costa, Ana Margarida Pereira, Margarida Casal, Raul Machado | Ano: 2022 | Database: pubmed

Resumo: In this study, novel antimicrobial biocomposite films comprising a genetically engineered silk-elastin protein polymer (SELP) and essential oil from Mentha piperita (MPEO) have been fabricated and tested for the antibacterial performance. SELP/MPEO biocomposite films were prepared by solvent casting using water as the solvent and aqueous emulsions of MPEO at different concentrations. Emulsions of MPEO were investigated, showing that the mixing method, relative amount of surfactant, and the presence of SELP influence particle size and homogeneity. The aqueous emulsions of SELP/MPEO were characterized by a population of particles between 100 and 300 nm, depending on the MPEO concentration. The emulsified oil droplets at the highest concentration showed to be homogeneously distributed into the SELP matrix and demonstrated antibacterial activity against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. Moreover, the antibacterial activity of the biocomposite films was retained after a period of storage for 7 days at 4 °C. The formulation of composites comprising natural active fillers and recombinant protein polymers opens opportunities to develop new green, functional biocomposite materials, paving the way for a new generation of multifunctional materials.

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Conclusão: N/A

Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model.

Autores: Marina Soković, Jasmina Glamočlija, Petar D Marin, Dejan Brkić, Leo J L D van Griensven | Ano: 2010 | Database: pubmed

Resumo: The chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: Citrus aurantium, C. limon, Lavandula angustifolia, Matricaria chamomilla, Mentha piperita, M. spicata, Ocimum basilicum, Origanum vulgare, Thymus vulgaris and Salvia officinalis have been determined. The antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed against the human pathogenic bacteria Bacillus subtilis, Enterobacter cloacae, Escherichia coli O157:H7, Micrococcus flavus, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, S. epidermidis, S. typhimurium, and Staphylococcus aureus. The highest and broadest activity was shown by O. vulgare oil. Carvacrol had the highest antibacterial activity among the tested components.

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Conclusão: N/A

Comparison of the antibacterial activity of essential oils and extracts of medicinal and culinary herbs to investigate potential new treatments for irritable bowel syndrome.

Autores: Aiysha Thompson, Dilruba Meah, Nadia Ahmed, Rebecca Conniff-Jenkins, Emma Chileshe, Chris O Phillips, Tim C Claypole, Dan W Forman, Paula E Row | Ano: 2013 | Database: pubmed

Resumo: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder, which may result from alteration of the gastrointestinal microbiota following gastrointestinal infection, or with intestinal dysbiosis or small intestinal bacterial overgrowth. This may be treated with antibiotics, but there is concern that widespread antibiotic use might lead to antibiotic resistance. Some herbal medicines have been shown to be beneficial, but their mechanism(s) of action remain incompletely understood. To try to understand whether antibacterial properties might be involved in the efficacy of these herbal medicines, and to investigate potential new treatments for IBS, we have conducted a preliminary study in vitro to compare the antibacterial activity of the essential oils of culinary and medicinal herbs against the bacterium, Esherichia coli.

Resultados: Most of the oils exhibited antibacterial activity in all three assays, however peppermint, lemon balm and coriander seed oils were most potent, with peppermint and coriander seed oils being more potent than the antibiotic rifaximin in the disc diffusion assay. The compounds present in these oils were identified by gas chromatography mass spectrometry. Finally, extracts were made of spearmint, lemon balm and coriander leaves with various solvents and these were tested for their antibacterial activity against E. coli in the disc diffusion assay. In each case, extracts made with ethanol and methanol exhibited potent antibacterial activity.

Conclusão: N/A

Inhibition by the essential oils of peppermint and spearmint of the growth of pathogenic bacteria.

Autores: H Imai, K Osawa, H Yasuda, H Hamashima, T Arai, M Sasatsu | Ano: 2001 | Database: pubmed

Resumo: The effects of the, essential oils of peppermint (Mentha piperita L.), spearmint Mentha spicata L.) and Japanese mint (Mentha, arvensis L.), of four major constituents of the esssential oil of peppermint, and of three major constituents of the essential oil of spearmint, on the proliferation of Helicobacter pylori, Salmonella enteritidis, Escherichia coli O157:H7, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococccus aureus (MSSA) were examined. The essential oils and the various constituents inhibited the proliferation of each strain in liquid culture in a dose-dependent manner. In addition, they exhibited bactericidal activity in phosphate-buffered saline. The antibacterial activities varied among the bacterial species tested but were almost the same against antibiotic-resistant and antibiotic-sensitive strains of Helicobacter pylori and S. aureus. Thus, the essential oils and their constituents may be useful as potential antibacterial agents for inhibition of the growth of pathogens.

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Conclusão: N/A

Antimicrobial action of essential oil vapours and negative air ions against Pseudomonas fluorescens.

Autores: A K Tyagi, A Malik | Ano: 2010 | Database: pubmed

Resumo: The aim of this study was to investigate the antibacterial activity of essential oil (in liquid as well as in vapour phase) and negative air ions (NAI) against Pseudomonas fluorescens. The combined effect of NAI with essential oil vapour was also investigated to determine kill time and morphological changes in bacterial cells. The MIC of Cymbopogon citratus (0.567 mg/ml), Mentha arvensis (0.567 mg/ml), Mentha piperita (1.125 mg/ml) and Eucalyptus globulus (2.25 mg/ml) was studied via the agar dilution method. To estimate the antibacterial activity of essential oils in the vapour phase, agar plates inoculated with P. fluorescens were incubated with various concentrations of each essential oil vapour and zone of inhibition was recorded. Further, in order to assess the kill time, P. fluorescens inoculated agar plates were exposed to selected bactericidal essential oil vapour and NAI, separately, in an air-tight chamber. A continuous decrease in bacterial count was observed over time. A significant enhancement in the bactericidal action was observed by exposure to the combination of essential oil vapour and NAI as compared to their individual action. Scanning electron microscopy was used to study the alteration in morphology of P. fluorescens cells after exposure to C. citratus oil vapour, NAI, and combination of C. citratus oil vapour and NAI. Maximum morphological deformation was found due to the combined effect of C. citratus oil vapour and NAI. This study demonstrates that the use of essential oils in the vapour phase is more advantageous than the liquid phase. Further the antibacterial effect of the essential oil vapours can be significantly enhanced by the addition of NAI. The work described here offers a novel and efficient approach for control of bacterial contamination that could be applied for food stabilization practices.

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Efeito Antibacteriano de Óleos Essenciais sobre Bactérias Formadoras do Biofilme Dentário/ Antibacterial Effect of Essential Oils on Biofilm-forming Bacterial

Autores: Alves, Lívia Araújo; Freires, Irlan de Almeida; Castro, Ricardo Dias de | Ano: 2010 | Database: LILACS

Resumo: Avaliar a atividade antibacteriana in vitro dos óleosessenciais de Eucalyptus globulus - Eucalipto (OE1), Eugeniauniflora L. - Pitangueira (OE2) e Mentha piperita - Hortelã-Pimenta (OE3) sobre bactérias cariogênicas. Material eMétodos: Determinou-se a Concentração Inibitória Mínima(CIM) dos óleos essenciais pela técnica da microdiluiçãosobre Streptococcus mutans (ATCC-25175), S. salivarius(ATCC-7073) e S. mitis (ATCC 903). Utilizou-se placa com 96orifícios, onde foram inseridos em cada um 10 ìL do inóculo(108 UFC/mL), 100 ìL de caldo Brain Heart Infusion (HIMEDIA,São Paulo, Brasil) duplamente concentrado e 100 ìL dos óleosessenciais em concentrações que variaram de 5 a 0,039mg.mL-1. Como controle positivo foi utilizada Clorexidina a 60ìg.mL-1. Ainda foram realizados os controles de crescimentobacteriano e de esterilidade do meio de cultura utilizado. Aleitura foi feita após 24 horas pelo método visual, sendoobservada a formação ou não de aglomerados de células(“botão”) no fundo da cavidade da placa. Resultados: O OE1inibiu crescimento bacteriano visível na concentração de 5mg.mL-1 sobre as três bactérias; OE2 mostrou CIM de 0,625mg.mL-1 e 3,75 mg.mL-1 sobre S. mitis e S. salivarius,respectivamente. OE3 exibiu CIM de 0,625 mg.mL-1, 0,312 mgmL-1 e 1,25 mg mL-1 sobre Streptococcus.mutans, S. mitis eS. salivarius, respectivamente. Conclusão: Os óleosessenciais avaliados apresentaram atividade antibacterianasobre as espécies cariogênicas ensaiadas...

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Effects of Olfactory Stimulation with Aroma Oils on Psychophysiological Responses of Female Adults.

Autores: Na-Yoon Choi, Yu-Tong Wu, Sin-Ae Park | Ano: 2022 | Database: pubmed

Resumo: This study investigated the effects of olfactory stimulation with aroma oils on the psychophysiological responses in women. Ten aromatic oils (lavender, rosemary, rose, eucalyptus, jasmine, geranium, chamomile, clary sage, thyme, and peppermint) were used on 23 women aged between 20 and 60 years. They inhaled the scent for 90 s through a glass funnel attached to their lab apron, 10 cm below their nose, while the pump was activated. Electroencephalography, blood pressure, and pulse rate were measured before and during inhalation of the aroma oils. The relative alpha (RA) power spectrums indicating relaxation and resting state of the brain significantly increased when lavender, rosemary, eucalyptus, jasmine, chamomile, clary sage, and thyme oils were inhaled compared to those of before olfactory stimulation. The ratio of alpha to high beta (RAHB), an indicator of brain stability and relaxation, significantly increased when rosemary, jasmine, clary sage, and peppermint oils were inhaled. The relative low beta (RLB) power spectrum, an indicator of brain activity in the absence of stress, significantly increased when stimulated with lavender, rosemary, rose, and geranium scents. Further, systolic blood pressure significantly decreased after introduction of all 10 types of aromatic oils, which indicates stress reduction. Thus, olfactory stimulation with aroma oil had a stabilizing effect on the prefrontal cortex and brain activity and decreased systolic blood pressure.

Resultados: N/A

Conclusão: N/A

Antiemetic activity of volatile oil from Mentha spicata and Mentha piperita in chemotherapy-induced nausea and vomiting.

Autores: Z Tayarani-Najaran, E Talasaz-Firoozi, R Nasiri, N Jalali, Mk Hassanzadeh | Ano: 2013 | Database: pubmed

Resumo: This study is aimed at determining the efficacy of Mentha spicata (M. spicata) and Mentha piperita (M. piperita) in preventing chemotherapy-induced nausea and vomiting (CINV).

Resultados: There was a significant reduction in the intensity and number of emetic events in the first 24 h with M. spicata and M. piperita in both treatment groups (p < 0.05) when compared with the control and no adverse effects were reported. The cost of treatment was also reduced when essential oils were used.

Conclusão: M. spicata or M. piperita essential oils are safe and effective for antiemetic treatment in patients, as well as being cost effective.

Mentha piperita L. essential oil inactivates spoilage yeasts in fruit juices through the perturbation of different physiological functions in yeast cells.

Autores: Erika Tayse da Cruz Almeida, Geany Targino de Souza, Jossana Pereira de Sousa Guedes, Isabella Medeiros Barbosa, Cristina Paiva de Sousa, Lúcio Roberto Cançado Castellano, Marciane Magnani, Evandro Leite de Souza | Ano: 2019 | Database: pubmed

Resumo: This study evaluated the efficacy of the essential oil from Mentha piperita L. (MPEO) to inactivate cells of the potentially spoilage yeasts Candida albicans, Candida tropicalis, Pichia anomala and Saccharomyces cerevisiae in cashew, guava, mango and pineapple juices during 72 h of refrigerated storage. Damage in different physiological functions caused by MPEO in S. cerevisiae in cashew and guava juices were investigated using flow cytometry (FC). The effects of the incorporation of an effective anti-yeast MPEO dose on sensory characteristics of juices were also evaluated. MPEO displayed minimum inhibitory concentration of 1.875 μL/mL against all tested yeasts. A >5 log reduction in counts of C. albicans, P. anomala and S. cerevisiae was observed in cashew and guava juices with 7.5 and 3.75 μL/mL MPEO. Tested MPEO concentrations (1.875, 3.75 and 7.5 μL/mL) were not effective to cause >5 log reduction in counts of target yeasts in mango and pineapple juices during 72 h of exposure. Incorporation of 1.875 μL/mL MPEO in cashew and guava juices strongly compromised membrane permeability, membrane potential, enzymatic activity and efflux pump activity in S. cerevisiae cells. This same MPEO concentration did not affect appearance, odor and viscosity in fruit juices, but negatively affected their taste and aftertaste. These results show the efficacy of MPEO to inactivate potentially spoilage yeasts in fruit juices through disturbance of different physiological functions in yeast cells. However, the combined use of MPEO with other technologies should be necessary to decrease its effective anti-yeast dose in fruit juices and, consequently, the possible negative impacts on specific sensory properties of these products.

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Conclusão: N/A

Screening of some essential oils against Trichosporon species.

Autores: Veena Uniyal, Seema Saxena, R P Bhatt | Ano: 2013 | Database: pubmed

Resumo: White Piedra is a superficial mycoses characterized by nodules on the hair shaft, caused by the basidiomycetous yeast Trichosporon species. In this study 25 essential oils were extracted and screened against two Trichosporon species i.e. Trichosporon asahii and Trichosporon cutaneum. Both these fungi procured from MTCC Chandigarh were maintained on yeast malt agar plates and tubes at 25 degrees C. Two screening methods viz., agar well diffusion assay and minimum inhibitory concentration were adopted for the study. The results showed that the maximum anti-yeast activity against T. asahii and T. cutaneum was demonstrated by oil of Mentha piperita showing full inhibition of both the fungi, Melaleuca alternifolia with an inhibition zone of 45 and 40 mm, Cymbopogon winterians with inhibition zone of 45 and 45 mm and Cymbopogon flexuosus with 35 and 30 mm inhibition zones. The oil of Trachyspermum ammi exhibited 10 and 20 mm, Abelmoschus moschatus exhibited 30 and 20 mm, Salvia sclarea showed 20 and 18 mm and Jasminum officinale exhibited 25 and 15 mm inhibition zones showing moderate activity. The oil of Cyperus scariosus, Pogostemon patchouli and Rosa damascene showed no inhibition zone against both the fungi while Vetiveria zizanoides exhibited no inhibition in case of T. asahii and inhibition zone of 10 mm in case of T. cutaneum demonstrating comparatively low activity against both the fungi. These results support that the essential oils can be used to cure superficial mycoses and these oils may have significant role as pharmaceuticals and preservatives.

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Conclusão: N/A

Screening da Atividade Antifúngica de Óleos Essenciais sobre Cepas de Candida/ Screening of Essential Oils Antifungal Activity on Candida Strains

Autores: Castro, Ricardo Dias de; Lima, Edeltrudes de Oliveira | Ano: 2011 | Database: LILACS

Resumo: Objetivo: O propósito desse estudo foi identificar a atividade antifúngica de óleos essenciais sobre cepas de Candida envolvidas com infecções da cavidade bucal. Método: Foram avaliados óleos essenciais obtidos a partir das seguintes espécies vegetais: Citrus reticulata (Tangerina Cravo); Citrus aurantifolia (Limão Tahiti); Cinnamomum zeylanicum (Canela); Matricaria chamomilla (Camomila Azul); Mentha piperita (Menta); Eugenia uniflora (Pitanga) e Zingiber officinale (Gengibre). A determinação da atividade antifúngica foi realizada utilizando a técnica de difusão em meio de cultura sólido, onde discos de papel de filtro foram embebidos nos óleos e colocados em placas de Petri contendo agar Sabouraud Dextrose inoculado com cepas de Candida albicans e C. tropicalis. Também foi observada a concentração inibitória mínima a partir do método da microdiluição. Os ensaios foram realizados em duplicata. Resultados: Foi observada expressiva atividade antifúngica dos óleos essenciais de C. zeylanicum, C. aurantifolia e M. piperita, que apresentaram diâmetros de halos de inibição de crescimento microbiano de até, respectivamente, 48 mm, 30 mm e 19 mm. Ainda foi possível identificar que 66,7% das cepas ensaiadas mostraram-se resistentes aos óleos essenciais de C. reticulata, M. chamomilla, E. uniflora e Z. officinale. O C. zeylanicum e nistatina apresentaram, respectivamente, CIMs de 312 µg mL-1 e 32 µg mL-1. Conclusão: Os óleos essenciais avaliados apresentam atividade antifúngica, sendo os melhores resultados encontrados para C. zeylanicum. Sugere-se a realização de outros ensaios para avaliação de atividade anti-Candida desse óleo essencial, que pode representar possível agente terapêutico no tratamento de infecções fúngicas da cavidade bucal

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Interactions between Clotrimazole and selected essential oils against Malassezia pachydermatis clinical isolates.

Autores: E Bohmova, E Conkova, M Harcarova, Z Sihelska | Ano: 2019 | Database: pubmed

Resumo: The aim of this study was to investigate interactions between conventional antifungal drug and essential oils against isolates of Malassezia pachydermatis. Antifungal activity of Cinnamomum cassia, Melaleuca alternifolia, Mentha piperita, Origanum vulgare and Syzygium aromaticum essential oils were tested against 19 strains of M. pachydermatis isolated from healthy dogs and reference strain M. pachydermatis CBS 1879. The checkerboard assay was used to search for in- teractions. Synergism was observed for the combination of clotrimazole with Melaleuca alternifolia essential oil, Mentha piperita and Origanum vulgare. The combinations of Cinnamomum cassia and Syzygium aromaticum essential oils with clotrimazole showed indifferent effect. Additive antimicrobial activity was observed for the combination of clotrimazole with Syzygium aromaticum and Melaleuca alternifolia essential oils against reference strain. The obtained results showed synergistic interactions between essential oils and clotrimazole which could improve effectiveness of this antifungal drug.

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Conclusão: N/A

Antifungal Activity of Essential Oils Against Candida Species Isolated from Clinical Samples.

Autores: S Córdoba, W Vivot, W Szusz, G Albo | Ano: 2019 | Database: pubmed

Resumo: We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l-1 for essential oils and antifungal drugs, respectively. MIC50 and MIC90, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l-1), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l-1) and intermediate for C. glabrata (MIC≤ 32 mg l-1). The essential oils showed antifungal activity on Candida spp. tested with MIC90 values ranging from 0.8 to 800 mg l-1. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC90 0.8-0.16 mg l-1), and the least active was C. officinalis (MIC90 400-800 mg l-1). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.

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Antifungal Activity of Essential Oils Against Candida Species Isolated from Clinical Samples.

Autores: S Córdoba, W Vivot, W Szusz, G Albo | Ano: 2019 | Database: pubmed

Resumo: We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l-1 for essential oils and antifungal drugs, respectively. MIC50 and MIC90, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l-1), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l-1) and intermediate for C. glabrata (MIC≤ 32 mg l-1). The essential oils showed antifungal activity on Candida spp. tested with MIC90 values ranging from 0.8 to 800 mg l-1. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC90 0.8-0.16 mg l-1), and the least active was C. officinalis (MIC90 400-800 mg l-1). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.

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Antifungal Activity of Some Essential Oils Emulsions Against Fungi Contaminating Ras Cheese.

Autores: A M Hassanin, S A Soliman, S A S Abdella, Hamdy A Shaaban | Ano: 2021 | Database: pubmed

Resumo: <b>Background and Objective:</b> Ras cheese is one of the important dairy products that are consumed in great quantities. But this cheese is vulnerable to the growth of fungi during ripening and selling until consumption. Therefore, research aimed to detect fungi contaminating Ras cheese and try to resist them. <b>Materials and Methods:</b> The effect of various concentrations (0.25, 0.5, 1, 2 and 3%) of essential oils emulsions of clove (<i>Syzygium aromaticum</i>), thyme (<i>Thymus vulgaris</i>) and peppermint (<i>Mentha piperita</i>) severally on the mycelial growth of the isolated fungi as compared to the control sample was tested <i>in vitro.</i> <b>Results:</b> The results indicated that many fungal species belonging to the genera <i>Aspergillus</i>, <i>Mucor</i>, <i>Eurotium</i> and <i>Mortierella</i> were isolated from the infected Ras cheese. Ochratoxin A was found in two samples whereas recorded the highest level in sample number 1 (2.1 μg kg¹). Aflatoxin M1 was found in few levels ranged between 0.012 and 0.360 μg kg¹in cheese samples, while aflatoxin B1 and B2 weren't detected in all samples. Clove essential oil emulsion completely inhibited the growth of all tested fungi at the concentration of 0.5%, followed by thyme essential oil emulsion which inhibited the fungal growth of all fungi at the concentration of 1%, while peppermint essential oil emulsion was less effective. <b>Conclusion:</b> The research recommends that clove and thyme essential oils emulsions can be used to resist the fungi of Ras cheese. Also, suggests that more research could be done on these essential oils emulsions to produce safe foods free of fungi.

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Interactions between Clotrimazole and selected essential oils against Malassezia pachydermatis clinical isolates.

Autores: E Bohmova, E Conkova, M Harcarova, Z Sihelska | Ano: 2019 | Database: pubmed

Resumo: The aim of this study was to investigate interactions between conventional antifungal drug and essential oils against isolates of Malassezia pachydermatis. Antifungal activity of Cinnamomum cassia, Melaleuca alternifolia, Mentha piperita, Origanum vulgare and Syzygium aromaticum essential oils were tested against 19 strains of M. pachydermatis isolated from healthy dogs and reference strain M. pachydermatis CBS 1879. The checkerboard assay was used to search for in- teractions. Synergism was observed for the combination of clotrimazole with Melaleuca alternifolia essential oil, Mentha piperita and Origanum vulgare. The combinations of Cinnamomum cassia and Syzygium aromaticum essential oils with clotrimazole showed indifferent effect. Additive antimicrobial activity was observed for the combination of clotrimazole with Syzygium aromaticum and Melaleuca alternifolia essential oils against reference strain. The obtained results showed synergistic interactions between essential oils and clotrimazole which could improve effectiveness of this antifungal drug.

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Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom.

Autores: F Diánez, M Santos, C Parra, M J Navarro, R Blanco, F J Gea | Ano: 2018 | Database: pubmed

Resumo: The antifungal properties of 12 Eos, that is, Syzygium aromaticum, Pelargonium graveolens, Lavandula angustifolia, Cupresus sempervirens, Mentha piperita, Santolina chamaecyparissus, Citrus sinensis, Pogostemon patchouli, Thymus mastichina, Thymus vulgaris, Eucalyptus globulus and Rosmarinus officinalis, were screened. The influence of five doses of each EOs was tested against Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora parasitica, Pythium aphanidermatum, Alternaria brassicae, Cladobotryum mycophilum and Trichoderma aggressivum f.sp. europaeum using disc-diffusion method. The mycelial growth inhibition and ED50 were calculated. The chemical analysis of the EOs was analysed using gas chromatography-mass spectroscopy. A total of 58 compounds were identified in the 12 EOs. All essential oils (EOs) analysed showed antifungal activity against the test pathogens in the range of 5·32-100%. The inhibitory effect of oils showed dose-dependent activity on the tested fungus. Based on the ED50 values, clove, rose geranium, peppermint and patchouli were the most effective. This study warrants further research into the practical use of EOs for the control of important myco- and phytopathogens in intensive horticulture.

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The in vitro effect of selected essential oils on the growth and mycotoxin production of Aspergillus species.

Autores: Miroslava Císarová, Dana Tančinová, Juraj Medo, Miroslava Kačániová | Ano: 2016 | Database: pubmed

Resumo: The aim of the present study was to assess the antifungal and anti-toxinogenic activity of 15 essential oils (EOs) against three fungi of the genus Aspergillus (A. parasiticus KMi-227-LR, A. parasiticus KMi-220-LR and A. flavus KMi-202-LR). The minimum inhibitory doses (MIDs) of the tested essential oils and their antifungal activity were determined using the micro-atmosphere method. The original commercial essential oil samples of Jasminum officinale L., Thymus vulgaris L., Syzygium aromaticum (L.) Merrill & Perry, Rosmarinus officinalis L., Ocimum basilicum L., Eucalyptus globulus Labill., Salvia officinalis L., Citrus limon (L.) Burm, Origanum vulgare L., Lavandula angustifolia Mill., Carum carvi L., Citrus sinensis (L.) Osbeck., Zingiber officinalis Rosc., Mentha piperita L. and Cinnamomum zeylanicum Nees. (C. verum J.S.Presl.) were produced in Slovakia (Calendula a.s., Nová Ľubovňa, Slovakia). All essential oils exhibited activity against all tested strains of fungi. After 14 days of incubation, A. flavus (KMi-202-LR) showed the highest susceptibility with a growth inhibition percentage (GIP) of 18.70% to C. limon and 5.92% to C. sinensis, while A. parasiticus (KMi-220-LR) exhibited a GIP of 20.56% to J. officinale. The minimum inhibitory doses (MIDs) of EOs with the most significant activity were recorded. The best antifungal activity, using the micro-atmosphere method was found in S. aromaticum with an MID of 62.5 μL L-1 air, T. vulgaris (MID of 62.5 μL L-1 air) and O. vulgare (MID of 31.5 μL L-1 air) against all tested strains. Mycotoxin production of the tested strains was evaluated by the thin layer chromatography (TLC) method. Mycotoxin production of AFB1 and AFG1 was inhibited following all treatments with C. carvi, R. officinale and S. officinale, Eucalyptus globulus L. and O. basilicum L. Essential oils exhibited a potential inhibition activity against toxic fungi, although, these affected only the production of AFB1.

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Antimicrobial activity of propolis and essential oils and synergism between these natural products

Autores: Probst, I. S; Sforcin, J. M; Rall, V. L. M; Fernandes, A. A. H; Fernandes Júnior, A | Ano: 2011 | Database: LILACS

Resumo: In the present study, Apis mellifera propolis and essential oils (EOs) obtained from aromatic plants were evaluated as alternative antimicrobials. We aimed to establish the antimicrobial activity of ethanolic extracts of propolis (EEP) from Apis mellifera and of EOs from Caryophyllus aromaticus, Zingiber officinale, Cinnamomum zeylanicum and Mentha piperita against 32 Staphylococcus aureus and Escherichia coli strains from human clinical specimens. The antimicrobials were diluted in agar and the minimal inhibitory concentrations (MIC) were found whereas MIC90 percent values were calculated. Time-kill curve assays using mixtures containing one quarter of MIC90 percent for EEP with all EOs as well cinnamon EO were performed. The cinnamon EO was found to be the most efficient, with MIC90 percent values of 1.22 and 0.96 mg x mL-1 respectively against S. aureus and E. coli, whereas MIC90 percent of EEP were 1.86 and 20.12 mg x mL-1 respectively against S. aureus and E. coli. The combinations of EEP with ginger and mint EOs, and cinnamon with ginger and clove EOs, showed synergistic effects. Consequently, further studies are necessary to confirm these activities in vivo and to evaluate the phytochemical characteristics of natural products.

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Chemical Composition and Antimicrobial Properties of Mentha piperita cv. 'Kristinka' Essential Oil.

Autores: Ippolito Camele, Daniela Gruľová, Hazem S Elshafie | Ano: 2021 | Database: pubmed

Resumo: Several economically important crops, fruits and vegetables are susceptible to infection by pathogenic fungi and/or bacteria postharvest or in field. Recently, plant essential oils (EOs) extracted from different medicinal and officinal plants have had promising antimicrobial effects against phytopathogens. In the present study, the potential microbicide activity of Mentha piperita cv. 'Kristinka' (peppermint) EO and its main constituents have been evaluated against some common phytopathogens. In addition, the cell membrane permeability of the tested fungi and the minimum fungicidal concentrations were measured. The antifungal activity was tested against the following postharvest fungi: Botrytis cinerea, Monilinia fructicola, Penicillium expansum and Aspergillus niger, whereas antibacterial activity was evaluated against Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas savastanoi and P. syringae pv. phaseolicola. The chemical analysis has been carried out using GC-MS and the main components were identified as menthol (70.08%) and menthone (14.49%) followed by limonene (4.32%), menthyl acetate (3.76%) and β-caryophyllene (2.96%). The results show that the tested EO has promising antifungal activity against all tested fungi, whereas they demonstrated only a moderate antibacterial effect against some of the tested bacteria.

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Peppermint Essential Oil-Doped Hydroxyapatite Nanoparticles with Antimicrobial Properties.

Autores: Monica Luminita Badea, Simona Liliana Iconaru, Andreea Groza, Mariana Carmen Chifiriuc, Mircea Beuran, Daniela Predoi | Ano: 2019 | Database: pubmed

Resumo: This study aimed at developing an antimicrobial material based on hydroxyapatite (HAp) and peppermint essential oil (P-EO) in order to stimulate the antimicrobial activity of hydroxyapatite. The molecular spectral features and morphology of the P-EO, HAp and hydroxyapatite coated with peppermint essential oil (HAp-P) were analyzed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The coating of the HAp with the P-EO did not affect the ellipsoidal shape of the nanoparticles. The overlapping of IR bands of P-EO and HAp in the HAp-P spectrum determined the formation of the broad molecular bands that were observed in the spectral regions of 400-1000 cm-1 and 1000-1200 cm-1. The antibacterial activity of the P-EO, HAp and HAp-P were also tested against different Gram-positive bacteria (methicillin-resistant Staphylococcus aureus (MRSA) 388, S. aureus ATCC 25923, S. aureus ATCC 6538, E. faecium DSM 13590), Gram-negative bacteria (Escherichia coli ATCC 25922, E. coli C5, P. aeruginosa ATCC 27853, P. aeruginosa ATCC 9027) and a fungal strain of Candida parapsilosis. The results of the present study revealed that the antimicrobial activity of HAp-P increased significantly over that of HAp.

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African peppermint (Mentha piperita) from Morocco: Chemical composition and antimicrobial properties of essential oil.

Autores: Chraibi Marwa, Kawtar Fikri-Benbrahim, Douae Ou-Yahia, Abdellah Farah | Ano: 2017 | Database: pubmed

Resumo: To replace and avoid synthetic chemicals toxicity, there is a growing interest in the investigation of natural products from plant origin for the discovery of active compounds with antimicrobial properties. This work was devoted to determine chemical composition and antimicrobial properties of the EO of M. piperita harvested in the garden of the National Institute of Medicinal and Aromatic Plants of Morocco. Experiments have been conducted at the Microbial Biotechnology Laboratory at the Sciences and Technology Faculty, Sidi Mohamed Ben Abdellah University, Fez, Morocco. M. piperita oil was screened for its antimicrobial activity against seven bacteria and two fungi using broth microdilution method. Chemical EO analysis was performed using CPG/MS. The EO revealed menthol (46.32%), menthofuran (13.18%), menthyl acetate (12.10%), menthone (7.42%), and 1,8-cineole (6.06%) as the main constituents. The tested EO exhibited strong inhibitory effect against all tested microorganisms with minimum inhibitory concentrations ranging from 0.062% to 0.5% (v/v), except for Pseudomonas aeruginosa that was the least sensitive and was only inhibited by concentrations as high as 0.5% (v/v). The studied EO showed an antimicrobial potential. This reinforces its use as an alternative to chemical additives that can be applied to the food and drug industry.

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Atividade antimccrobiana de óleos essenciais de plantas medicinais/ Actividad antmiccrobiana de aceites esenciales de plantas medicinaees/ Antimicrbial acttivity of medicinal plant essential oils

Autores: Geromini, Kassia Vanessa Navarro; Roratto, Fernando Brito; Ferreira, Fabio Gomes; Polido, Polyana Barros; Souza, Silvia Graciele Hülse de; Valle, Juliana Silveira do; Colauto, Nelson Barros; Linde, Giani Andrea | Ano: 2012 | Database: LILACS

Resumo: O objetivo deste trabalho foi avaliar a atividade antimicrobiana dos óleos essenciais de Lippia alba (erva-cidreira-brasileira), Mentha piperita (hortelã-pimenta), Ocimum gratissimum (alfavaca-cravo) e Rosmarinus officinalis (alecrim) para Candida albicans, Escherichia coli, Staphylococcus aureus e Pseudomonas aeruginosa. Os óleos essenciais foram obtidos de folhas por hidrodestilação. O rendimento de extração do óleo em mL kg-1 foi de 1,5; 1,9; 2,0 e 2,3 para L. alba; M. piperita; O. gratissimum e R. officinalis, respectivamente. Os micro-organismos foram padronizados a 0,5 na escala McFarland e crescidos em ágar Mueller-Hinton. Discos de papel embebidos nos óleos essenciais foram posicionados na superfície do meio de cultivo e os halos de inibição do crescimento foram medidos. Os óleos essenciais, principalmente de L. alba e O. gratissimum, demonstraram alto potencial inibitório sobre o crescimento dos micro-organismos avaliados, com exceção de P. aeruginosa que apresentou alta resistência à presença dos óleos essenciais.

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Antimicrobial activity of Mentha piperita L. against Candida spp/ Atividade antimicrobiana de Mentha piperita L. sobre Candida spp

Autores: Carretto, Claunencil de Fátima Pires; Almeida, Rosilene Batista de Aguiar; Furlan, Marcos Roberto; Jorge, Antonio Olavo Cardoso; Junqueira, Juliana Campos | Ano: 2010 | Database: LILACS

Resumo: The aim of this study was to investigate the in vitro effects of a hydroalcoholic extract, an infusion and the essential oil from Mentha piperita L. on the growth of Candida spp. The hydroalcoholic extract and essential oil were evaluated for antimicrobial activity against 50 strains of Candida albicans, 10 strains of Candida glabrata, 10 strains of Candida tropicalis, 8 strains of Candida parapsilosis and 2 strains of Candida krusei. The minimum inhibitory concentration (MIC) of the hydroalcoholic extract was determined by the broth dilution method, and the antimicrobial activity of the essential oil was determined by the agar diffusion test. The effect of an infusion of Mentha piperita L. on the adherence of standard Candida strains to acrylic resin was also investigated. The results demonstrated that the hydroalcoholic extract of Mentha piperita L. showed fungistatic activity against the strains C. albicans, C. tropicalis and C. glabrata. The essential oil showed the strongest inhibitory activity against the strains C. albicans, C. tropicalis and C. parapsilosis. Despite this inhibitory activity, an infusion of Mentha piperita L. did not inhibit the adherence of Candida to acrylic resin. In conclusion, Mentha piperita L. presented significant antimicrobial activity against the strains C. albicans and C. tropicalis but showed no effect on the adherence of these microorganisms to acrylic resin.

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Comparative study of antimicrobial activity between some medicine plants and recombinant Lactoferrin peptide against some pathogens of cultivated button mushroom.

Autores: Abbas Tanhaeian, Narges Nazifi, Farajollah Shahriari Ahmadi, Mahdi Akhlaghi | Ano: 2020 | Database: pubmed

Resumo: The adverse effects of chemical pesticides on human health and environment cannot be ignored, hence it seems that novel alternative compounds should be applied to control plant pathogens. Among various alternative sources, natural compounds such as plant essential oils, plant extracts and recombinant antimicrobial peptides are of significance. The aim of the present study was to investigate antimicrobial activity of plants essential oils and plant extracts of six medicinal plants (Lippia citriodora, Ferula gummosa, Bunium persicum, Mentha piperita, Plantago major and Salvadora persica) along with a chimera peptide of camel lactoferrin, which is the most important antimicrobial component of camel milk, against Pseudomonas tolaasii and Trichoderma harzianum as pathogens of white button mushroom. The antibacterial activity test was conducted under in vitro conditions through disc diffusion method. The results showed that chimera camel lactoferrin peptide, with the highest amount of inhibitory zone (14.63 mm in 20 μg/mL concentration), has a significant difference in antibacterial activity compared to other treatments. Ferula gummosa conferred no antibacterial activity. Also, the results of antifungal effects indicated that plant essential oils and extracts have more antifungal activity than recombinant peptide. Generally, L. citriodora, B. persicum, M. piperita treatments could completely prevent growth of fungal in in vitro conditions. Therefore, using the mentioned plants can be a good replacement for reducing the chemical pesticides against pathogenic agents of button mushroom, without any adverse effects on environment and human health.

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Atividade antimicrobiana de plantas medicinais indicadas para uso no Sistema Único de Saúde/ Actividad antimicrobiana de las plantas medicinales para su uso en el Sistema Único de Salud/ Antimicrobial activity of medicinal plants for their potential use in the Brazilian Unified Health System

Autores: Feitosa Alves, Vanessa; Dantas Figueiredo, Rebeca; Wanderley Cavalcanti, Yuri; Nascimento Padilha, Wilton Wilney | Ano: 2019 | Database: LILACS

Resumo: RESUMO Introdução: No Brasil, uma lista contendo 71 ervas medicinais foi divulgada pelo Ministério da Saúde com intuito de conduzir pesquisas e desenvolvimento de fitoterápicos para uso no Sistema Único de Saúde. Objetivo: Avaliar in vitro a atividade antimicrobiana de extratos de ervas medicinais sugeridos pelo Ministério da Saúde para utilização no Sistema Único de Saúde frente a bactérias orais. Métodos: Os extratos vegetais selecionados estavam na forma de óleo essencial (Eucalyptus globulus, Mentha piperita e Schinus terebinthifolius) ou tintura (Erythrina mulungu, Casearia sylvestris e Maytenus ilicifolia) e foram avaliados sobre Streptococcus mutans (ATCC 25175), S. oralis (ATCC 10557) e S. salivarius (ATCC 7073). Realizou-se o teste de difusão em ágar com confecção de poços e adição de 50 µL dos extratos. Após 48 h em estufa bacteriológica, os halos de inibição foram medidos através de paquímetro. Para determinação da concentração inibitória mínima e da concentração bactericida mínima, realizou-se a técnica de microdiluição em microplacas e de esgotamento, respectivamente. A concentração inibitória mínima correspondeu a menor diluição na qual se verificou ausência de crescimento bacteriano visível. O controle positivo utilizado foi Clorexidina 0,12 porcento. Todos os testes foram realizados em triplicata e analisados descritivamente. Resultados: O extrato de Maytenus ilicifolia apresentou halos de inibição discretamente maiores aos demais produtos. Erythrina mulungu apresentou a menor da concentração inibitória mínima frente S. mutans (2,81 mg/mL) entre as tinturas e Mentha piperita (9,00), entre os óleos essenciais. Erythrina mulungu e Mentha piperita foram os únicos extratos que apresentaram concentração bactericida mínima frente às cepas utilizadas. Conclusão: Os extratos vegetais apresentaram atividade antimicrobiana frente a microrganismos orais destacando Mentha piperita e Erythrina mulungu(AU)

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Antimicrobial activities of six essential oils commonly used as condiments in Brazil against Clostridium perfringens

Autores: Radaelli, Marcela; Silva, Bárbara Parraga da; Weidlich, Luciana; Hoehne, Lucélia; Flach, Adriana; Costa, Luiz Antonio Mendonça Alves da; Ethur, Eduardo Miranda | Ano: 2016 | Database: LILACS

Resumo: Abstract Despite recent advances in food production technology, food-borne diseases (FBD) remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil), Rosmarinus officinalis L. (rosemary), Origanum majorana L. (marjoram), Mentha × piperita L. var. Piperita (peppermint), Thymus vulgaris L. (thyme) and Pimpinella anisum L. (anise) against C. perfringens strain A. Chemical compositions of the oils were determined by GC–MS (gas chromatography–mass spectrometry). The identities of the isolated compounds were established from the respective Kováts indices, and a comparison of mass spectral data was made with those reported earlier. The antibacterial activity was assessed from minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. Minimum inhibitory concentration values were 1.25 mg mL-1 for thyme, 5.0 mg mL-1 for basil and marjoram, and 10 mg mL-1 for rosemary, peppermint and anise. All oils showed bactericidal activity at their minimum inhibitory concentration, except anise oil, which was only bacteriostatic. The use of essential oils from these common spices might serve as an alternative to the use of chemical preservatives in the control and inactivation of pathogens in commercially produced food systems.

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Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil

Autores: Sartoratto, Adilson; Machado, Ana Lúcia M; Delarmelina, Camila; Figueira, Glyn Mara; Duarte, Marta Cristina T; Rehder, Vera Lúcia G | Ano: 2004 | Database: LILACS

Resumo: Oleos essenciais foram obtidos a partir das partes aéreas de Mentha piperita, M. spicata, Thymus vulgaris, Origanum vulgare, O. applii, Aloysia triphylla, Ocimum gratissimum e O. basilicum através de arraste de vapor em sistema tipo Clevenger. Os óleos foram avaliados quanto à atividade antimicrobiana contra bactérias e contra a levedura Candida albicans pelo método de bioautografia. A concentração mínima inibitória dos óleos com atividade positiva foi em seguida determinada pelo método da microdiluição. De acordo com os resultados, a maioria dos óleos essenciais estudados foram efetivos contra Enterococcus faecium e Salmonella cholerasuis. A.triphylla e O. basilicum apresentaram inibição moderada contra Staphylococcus aureus enquanto apenas A. tryphila e M. piperita foram capazes de inibir a levedura Candida albicans. Os óleos foram analisados quimicamente por técnicas de CG e CG-EM de modo a determinar os compostos majoritários presentes.

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Atividade antimicrobiana de óleos essenciais em bactérias patogênicas de origem alimentar/ Antimicrobial activity of essential oils against sessile and planktonic pathogens of food source

Autores: Valeriano, C.; Piccoli, R.H.; Cardoso, M.G.; Alves, E. | Ano: 2012 | Database: LILACS

Resumo: Objetivou-se identificar e quantificar os constituintes e avaliar a atividade antimicrobiana dos óleos essenciais de Mentha piperita, Cymbopogon citratus, Ocimum basilicum e Origanum majorana contra cepas de Escherichia coli enteropatogênica, Salmonella enterica Enteritidis, Listeria monocytogenes e Enterobacter sakazaki. A obtenção dos óleos essenciais foi realizada a partir de folhas secas, empregando-se a técnica de hidrodestilação e utilizando-se a aparelho de Clevenger modificado. A atividade antibacteriana dos óleos essenciais foi determinada pelo método de difusão em ágar. Observou-se que os óleos essenciais inibiram o crescimento bacteriano, mas a efetividade foi variada. Entre os óleos essenciais testados, M. piperita apresentou maior atividade antibacteriana para E. coli, (8.106 UA mL-1) quando comparada as demais bactérias, atividade moderada para Salmonella enterica Enteritidis e Enterobacter sakazakii (1.706 e 3.200 UA mL-1 respectivamente) e baixa atividade para Listeria monocytogenes (106,67 UA mL-1). Já óleo essencial de Cymbopogon citratus apresentou maior atividade antimicrobiana frente a E. coli (9.386 UA mL-1) e atividade moderada frente a Enterobacter sakazakii, Salmonella enterica Enteritidis e Listeria monocytogenes (2.773 UA mL-1 para ambas). Ocimum basilicum apresentou maior atividade antibacteriana frente E. coli e Enterobacter sakazakii (6.826 e 8.106 UA mL-1 respectivamente), moderada atividade frente a Salmonella enterica Enteritidis (1.600 UA mL-1) e não apresentou atividade frente a Listeria monocytogenes.Origanum majorana também foi testado neste estudo e apresentou maior atividade antimicrobiana frente E. coli (5.973 UA mL-1), atividade moderada para Salmonella enterica Enteritidis e Enterobacter sakazakii (1.706 e 2.346 UA mL-1 , respectivamente) e não apresentou atividade para Listeria monocytogenes.

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Conclusão: N/A

Antimicrobial synergism and antibiofilm activities of Pelargonium graveolens, Rosemary officinalis, and Mentha piperita essential oils against extreme drug-resistant Acinetobacter baumannii clinical isolates.

Autores: Ayse Humeyra Taskin Kafa, Rukiye Aslan, Cem Celik, Mursit Hasbek | Ano: 2022 | Database: pubmed

Resumo: Rosemary officinalis L., Pelargonium graveolens L., and Mentha piperita L., essential oils are used by complementary medicine specialists simultaneously with traditional antibiotics for treatment purposes. The chemical composition of essential oils was analyzed by the gas chromatography-mass spectrometry method. In vitro antibacterial and antibiofilm activities of the essential oils were tested against extreme drug-resistant (XDR) colistin-resistant and colistin susceptible Acinetobacter baumannii clinical strains. The synergistic activities between essential oils and colistin antibiotics were investigated by the checkerboard method. The highest antibacterial effect was detected in mint essential oil (2.5-5 μl/ml), followed by pelargonium essential oil (5-20 μl/ml) and rosemary essential oil (5-20 μl/ml). The combination of rosemary essential oil or pelargonium essential oil with colistin showed strong synergistic activity in most of the bacterial strains tested (fractional inhibitory concentration index ≤ 0.5; synergy). As a result of the combination of mint essential oil and colistin, an indifferent effect was observed in only two bacterial strains, and other strains could not be evaluated. No antagonistic effects were observed in any of the tested essential oils. As a result of the effectiveness of the combination, the minimum inhibitory concentration (MIC) values of colistin in XDR-A. baumannii clinical isolates decreased 2-32 fold. Additionally, the sub-MIC concentration of essential oils exhibited an inhibitory effect (48-90%) against the biofilm layer of tested A. baumannii strains.

Resultados: N/A

Conclusão: N/A

Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils.

Autores: Davod Yadegarinia, Latif Gachkar, Mohammad Bagher Rezaei, Massoud Taghizadeh, Shakiba Alipoor Astaneh, Iraj Rasooli | Ano: 2006 | Database: pubmed

Resumo: GC-MS analysis of essential oils of Iranian Mentha piperita and Myrtus communis extracted by hydrodistillation lead to identification of 26 and 32 compounds, respectively. The oils had good to excellent antimicrobial activities against Escherichia coli, Staphylococcus aureus and Candida albicans with the oil of M. piperita being more active. The findings suggest feasibility of application of M. piperita oil in treatment of the infections caused by C. albicans and E. coli. D-values on exposure to M. piperita and Myrtus communis oils were (2.14 and 2.8min), (1.4 and 12.8min) and (4.3 and 8.6min) for E. coli, S. aureus and C. albicans , respectively. The oils were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical scavenging and beta-carotene/linoleic acid systems. M. piperirta oil exerted greater antioxidant activity than that of M. communis. Phytochemical and phytobiological characteristics of these oils may lead to extraction and production of active compounds in single or combined forms with useful applications.

Resultados: N/A

Conclusão: N/A

Plant elicitation and TiO2 nanoparticles application as an effective strategy for improving the growth, biochemical properties, and essential oil of peppermint.

Autores: Akram Shenavaie Zare, Ali Ganjeali, Mohammad Reza Vaezi Kakhki, Monireh Cheniany, Mansour Mashreghi | Ano: 2022 | Database: pubmed

Resumo: Mentha piperita L., which is an abundant source of essential oils (EO) and phenolic acids, is well known for its medicinal significance. The present research aimed to evaluate the impact of various concentrations of methyl jasmonate (MeJA; 0, 0.1, and 0.5 mM), titanium dioxide nanoparticles (TiO2 NPs; 0 and 150 mg L-1), and salicylic acid (SA; 0, 0.1, and 1 mM) on growth, EOs, and phenolic compounds of M. piperita L. The results demonstrated that the simultaneous application of SA (0.1 mM) and TiO2 NPs (150 mg L-1) enhanced shoot dry weight, the shoot length, and membrane stability index of peppermint by 56.17, 19.52, and 36%, respectively, compared to control. Moreover, phenolic content (76%), caffeic acid content (78%), rosmarinic acid content (87%), 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability (78%), and catalase (155%), ascorbate peroxidase activities (95%) were further improved by simultaneously applying MeJA (0.1 mM) and TiO2 NPs (150 mg L-1) compared to control. The highest menthol production (44.51%) was obtained with exogenous application of MeJA (0.1 mM) with 150 mg L-1 TiO2 NPs. The findings of the current study presented an ideal combination of TiO2 NPs with plant growth regulators for promoting antioxidant activities and increasing major components of EO in peppermint plants.

Resultados: N/A

Conclusão: N/A

Identification of essential oils with activity against stationary phase Staphylococcus aureus.

Autores: Shuzhen Xiao, Peng Cui, Wanliang Shi, Ying Zhang | Ano: 2020 | Database: pubmed

Resumo: Staphylococcus aureus is the most dominant human pathogen, responsible for a variety of chronic and severe infections. There is mounting evidence that persisters are associated with treatment failure and relapse of persistent infections. While some essential oils were reported to have antimicrobial activity against growing S. aureus, activity of essential oils against the stationary phase S. aureus enriched in persisters has not been investigated.

Resultados: We identified 39 essential oils (Oregano, Cinnamon bark, Thyme white, Bandit "Thieves", Lemongrass (Cymbopogon flexuosus), Sandalwood oil, Health shield, Allspice, Amyris, Palmarosa, Cinnamon leaf, Clove bud, Citronella, Geranium bourbon, Marjoram, Peppermint, Lemongrass, Cornmint, Elemi, Ho wood, Head ease, Lemon eucalyptus, Litsea cubeba, Myrrh, Parsley seed, Coriander oil, Dillweed, Hyssop, Neroli, Rosewood oil, Tea tree, Cajeput, Clove bud, Lavender, Sleep tight, Vetiver, Palo santo, Sage oil, Yarrow) at 0.5% (v/v) concentration, 10 essential oils (Cinnamon bark, Oregano, Thyme white, Bandit "Thieves", Lemongrass, Sandalwood oil, Health shield, Allspice, Amyris, Palmarosa at 0.25% (v/v) concentration, and 7 essential oils (Oregano, Cinnamon bark, Thyme white, Lemongrass, Allspice, Amyris, Palmarosa at 0.125% (v/v) concentration to have high activity against stationary phase S. aureus with no visible growth on agar plates after five-day exposure. Among the 10 essential oils which showed high activity at 0.25% (v/v) concentration, 9 (Oregano, Cinnamon bark, Thyme white, Bandit "Thieves", Lemongrass, Health shield, Allspice, Palmarosa, Amyris showed higher activity than the known persister drug tosufloxacin, while Sandalwood oil had activity at a higher concentration. In Oregano essential oil combination studies with antibiotics, Oregano plus tosufloxacin (or levofloxacin, ciprofloxacin) and rifampin completely eradicated stationary phase S. aureus cells, but had no apparent enhancement for linezolid, vancomycin, sulfamethoxazole, trimethoprim, azithromycin or gentamicin.

Conclusão: N/A

Nematicidal activity of mint aqueous extracts against the root-knot nematode Meloidogyne incognita.

Autores: Pierluigi Caboni, Marco Saba, Graziella Tocco, Laura Casu, Antonio Murgia, Andrea Maxia, Urania Menkissoglu-Spiroudi, Nikoletta Ntalli | Ano: 2013 | Database: pubmed

Resumo: The nematicidal activity and chemical characterization of aqueous extracts and essential oils of three mint species, namely, Mentha piperita , Mentha spicata , and Mentha pulegium , were investigated. The phytochemical analysis of the essential oils was performed by means of GC-MS, whereas the aqueous extracts were analyzed by LC-MS. The most abundant terpenes were isomenthone, menthone, menthol, pulegone, and carvone, and the water extracts yielded mainly chlorogenic acid, salvianolic acid B, luteolin-7-O-rutinoside, and rosmarinic acid. The water extracts exhibited significant nematicidal activity against Meloidogyne incognita , and the EC50/72h values were calculated at 1005, 745, and 300 mg/L for M. piperita, M. pulegium, and M. spicata, respectively. Only the essential oil from M. spicata showed a nematicidal activity with an EC50/72h of 358 mg/L. Interestingly, menthofuran and carvone showed EC50/48h values of 127 and 730 mg/L, respectively. On the other hand, salicylic acid, isolated in the aqueous extracts, exhibited EC50 values at 24 and 48 h of 298 ± 92 and 288 ± 79 mg/L, respectively.

Resultados: N/A

Conclusão: N/A

Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils.

Autores: Davod Yadegarinia, Latif Gachkar, Mohammad Bagher Rezaei, Massoud Taghizadeh, Shakiba Alipoor Astaneh, Iraj Rasooli | Ano: 2006 | Database: pubmed

Resumo: GC-MS analysis of essential oils of Iranian Mentha piperita and Myrtus communis extracted by hydrodistillation lead to identification of 26 and 32 compounds, respectively. The oils had good to excellent antimicrobial activities against Escherichia coli, Staphylococcus aureus and Candida albicans with the oil of M. piperita being more active. The findings suggest feasibility of application of M. piperita oil in treatment of the infections caused by C. albicans and E. coli. D-values on exposure to M. piperita and Myrtus communis oils were (2.14 and 2.8min), (1.4 and 12.8min) and (4.3 and 8.6min) for E. coli, S. aureus and C. albicans , respectively. The oils were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical scavenging and beta-carotene/linoleic acid systems. M. piperirta oil exerted greater antioxidant activity than that of M. communis. Phytochemical and phytobiological characteristics of these oils may lead to extraction and production of active compounds in single or combined forms with useful applications.

Resultados: N/A

Conclusão: N/A

Enhancing the Antimicrobial Effect of Ozone with Mentha piperita Essential Oil.

Autores: Alin-Daniel Floare, Ramona Dumitrescu, Vlad Tiberiu Alexa, Octavia Balean, Camelia Szuhanek, Diana Obistioiu, Ileana Cocan, Alina-Georgeta Neacsu, Iuliana Popescu, Aurora Doris Fratila, Atena Galuscan | Ano: 2023 | Database: pubmed

Resumo: This study aimed to obtain and analyse Mentha piperita essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time-dose relationships and time-effect correlations. Mentha piperita (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time-dose relationship and specific t-test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: S. aureus > P. aeruginosa > E. coli > C. albicans > S. mutans. For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: C. albicans > E. coli > P. aeruginosa > S. aureus > S. mutans. The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.

Resultados: N/A

Conclusão: N/A

Chromosome doubling influences the morphological, physiological, biochemical and genetic traits related to essential oil biosynthesis of peppermint (Mentha piperita) under salinity stress.

Autores: Zhongjuan Zhao, Yanli Wei, Ling Li, Baojun Liu, Kai Yang, Hetong Yang, Jishun Li | Ano: 2022 | Database: pubmed

Resumo: Peppermint (Mentha piperita L.) is an important medicinal aromatic plant. In this study, the morphology, physiology, biochemistry and gene expression of chromosomes doubling peppermint (D1 lines) were analyzed. The analysis showed that D1 lines had larger, thicker and darker leaves, and stronger roots when planted in the pots, but delayed growth in the field condition. Under NaCl stress, the D1 lines increased cell oxidative defense through more active antioxidant enzymes and decreased the oxidative damages of cell membrane, leading to a significantly greater survival rate and photosynthesis intensity than WT lines. The size and density of glandular trichomes of D1 lines was larger, which contributed to its higher essential oil yield. In addition, chromosome doubling reduced the inhibition of NaCl stress on essential oil yield and quality, through changing the expression of genes in the oil biosynthesis pathway. The traits of chromosome doubling peppermint provide new technical and theoretical evidence for peppermint germplasm improvement.

Resultados: N/A

Conclusão: N/A

Effect of the ultrasound-assisted preliminary maceration on the efficiency of the essential oil distillation from selected herbal raw materials.

Autores: Radosław Kowalski, Grażyna Kowalska, Jerzy Jamroz, Agnieszka Nawrocka, Damian Metyk | Ano: 2015 | Database: pubmed

Resumo: The objective of the study was the estimation of the ultrasound-assisted preliminary maceration effect on the efficiency of essential oil distillation and on its qualitative and quantitative composition. The experiment included analyses on three herbal materials, i.e. peppermint leaves (Mentha piperita L.), marjoram herb (Origanum majorana L.), and chamomile flowers (Chamomilla recutita (L.) Rauschert). The study showed that the application of preliminary water maceration of raw material, augmented with ultrasounds, had a statistically significant increasing effect on the amount of oil distilled, in the case of mint leaf from 1.32% to 1.46% v/w, and in the case of marjoram herb from 1.13% to 1.27% v/w. In the case of chamomile flowers no significant effect of ultrasound on the amount of oil obtained was observed. Generally, comparing the composition of essential oils obtained in the experiments with the requirements of the relevant standards no unfavourable effect of the distillation augmenting techniques applied was noted. Therefore, it should be expected that the studied distillates of volatile fractions will have the desired biological activity.

Resultados: N/A

Conclusão: N/A

Plant elicitation and TiO2 nanoparticles application as an effective strategy for improving the growth, biochemical properties, and essential oil of peppermint.

Autores: Akram Shenavaie Zare, Ali Ganjeali, Mohammad Reza Vaezi Kakhki, Monireh Cheniany, Mansour Mashreghi | Ano: 2022 | Database: pubmed

Resumo: Mentha piperita L., which is an abundant source of essential oils (EO) and phenolic acids, is well known for its medicinal significance. The present research aimed to evaluate the impact of various concentrations of methyl jasmonate (MeJA; 0, 0.1, and 0.5 mM), titanium dioxide nanoparticles (TiO2 NPs; 0 and 150 mg L-1), and salicylic acid (SA; 0, 0.1, and 1 mM) on growth, EOs, and phenolic compounds of M. piperita L. The results demonstrated that the simultaneous application of SA (0.1 mM) and TiO2 NPs (150 mg L-1) enhanced shoot dry weight, the shoot length, and membrane stability index of peppermint by 56.17, 19.52, and 36%, respectively, compared to control. Moreover, phenolic content (76%), caffeic acid content (78%), rosmarinic acid content (87%), 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability (78%), and catalase (155%), ascorbate peroxidase activities (95%) were further improved by simultaneously applying MeJA (0.1 mM) and TiO2 NPs (150 mg L-1) compared to control. The highest menthol production (44.51%) was obtained with exogenous application of MeJA (0.1 mM) with 150 mg L-1 TiO2 NPs. The findings of the current study presented an ideal combination of TiO2 NPs with plant growth regulators for promoting antioxidant activities and increasing major components of EO in peppermint plants.

Resultados: N/A

Conclusão: N/A

Enhancing the Antimicrobial Effect of Ozone with Mentha piperita Essential Oil.

Autores: Alin-Daniel Floare, Ramona Dumitrescu, Vlad Tiberiu Alexa, Octavia Balean, Camelia Szuhanek, Diana Obistioiu, Ileana Cocan, Alina-Georgeta Neacsu, Iuliana Popescu, Aurora Doris Fratila, Atena Galuscan | Ano: 2023 | Database: pubmed

Resumo: This study aimed to obtain and analyse Mentha piperita essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time-dose relationships and time-effect correlations. Mentha piperita (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time-dose relationship and specific t-test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: S. aureus > P. aeruginosa > E. coli > C. albicans > S. mutans. For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: C. albicans > E. coli > P. aeruginosa > S. aureus > S. mutans. The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.

Resultados: N/A

Conclusão: N/A

Improving peppermint essential oil yield and composition by metabolic engineering.

Autores: Bernd Markus Lange, Soheil Seyed Mahmoud, Mark R Wildung, Glenn W Turner, Edward M Davis, Iris Lange, Raymond C Baker, Rick A Boydston, Rodney B Croteau | Ano: 2011 | Database: pubmed

Resumo: Peppermint (Mentha piperita L.) was transformed with various gene constructs to evaluate the utility of metabolic engineering for improving essential oil yield and composition. Oil yield increases were achieved by overexpressing genes involved in the supply of precursors through the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway. Two-gene combinations to enhance both oil yield and composition in a single transgenic line were assessed as well. The most promising results were obtained by transforming plants expressing an antisense version of (+)-menthofuran synthase, which is critical for adjusting the levels of specific undesirable oil constituents, with a construct for the overexpression of the MEP pathway gene 1-deoxy-D-xylulose 5-phosphate reductoisomerase (up to 61% oil yield increase over wild-type controls with low levels of the undesirable side-product (+)-menthofuran and its intermediate (+)-pulegone). Elite transgenic lines were advanced to multiyear field trials, which demonstrated consistent oil yield increases of up to 78% over wild-type controls and desirable effects on oil composition under commercial growth conditions. The transgenic expression of a gene encoding (+)-limonene synthase was used to accumulate elevated levels of (+)-limonene, which allows oil derived from transgenic plants to be recognized during the processing of commercial formulations containing peppermint oil. Our study illustrates the utility of metabolic engineering for the sustainable agricultural production of high quality essential oils at a competitive cost.

Resultados: N/A

Conclusão: N/A

Improving peppermint essential oil yield and composition by metabolic engineering.

Autores: Bernd Markus Lange, Soheil Seyed Mahmoud, Mark R Wildung, Glenn W Turner, Edward M Davis, Iris Lange, Raymond C Baker, Rick A Boydston, Rodney B Croteau | Ano: 2011 | Database: pubmed

Resumo: Peppermint (Mentha piperita L.) was transformed with various gene constructs to evaluate the utility of metabolic engineering for improving essential oil yield and composition. Oil yield increases were achieved by overexpressing genes involved in the supply of precursors through the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway. Two-gene combinations to enhance both oil yield and composition in a single transgenic line were assessed as well. The most promising results were obtained by transforming plants expressing an antisense version of (+)-menthofuran synthase, which is critical for adjusting the levels of specific undesirable oil constituents, with a construct for the overexpression of the MEP pathway gene 1-deoxy-D-xylulose 5-phosphate reductoisomerase (up to 61% oil yield increase over wild-type controls with low levels of the undesirable side-product (+)-menthofuran and its intermediate (+)-pulegone). Elite transgenic lines were advanced to multiyear field trials, which demonstrated consistent oil yield increases of up to 78% over wild-type controls and desirable effects on oil composition under commercial growth conditions. The transgenic expression of a gene encoding (+)-limonene synthase was used to accumulate elevated levels of (+)-limonene, which allows oil derived from transgenic plants to be recognized during the processing of commercial formulations containing peppermint oil. Our study illustrates the utility of metabolic engineering for the sustainable agricultural production of high quality essential oils at a competitive cost.

Resultados: N/A

Conclusão: N/A

Effect of the ultrasound-assisted preliminary maceration on the efficiency of the essential oil distillation from selected herbal raw materials.

Autores: Radosław Kowalski, Grażyna Kowalska, Jerzy Jamroz, Agnieszka Nawrocka, Damian Metyk | Ano: 2015 | Database: pubmed

Resumo: The objective of the study was the estimation of the ultrasound-assisted preliminary maceration effect on the efficiency of essential oil distillation and on its qualitative and quantitative composition. The experiment included analyses on three herbal materials, i.e. peppermint leaves (Mentha piperita L.), marjoram herb (Origanum majorana L.), and chamomile flowers (Chamomilla recutita (L.) Rauschert). The study showed that the application of preliminary water maceration of raw material, augmented with ultrasounds, had a statistically significant increasing effect on the amount of oil distilled, in the case of mint leaf from 1.32% to 1.46% v/w, and in the case of marjoram herb from 1.13% to 1.27% v/w. In the case of chamomile flowers no significant effect of ultrasound on the amount of oil obtained was observed. Generally, comparing the composition of essential oils obtained in the experiments with the requirements of the relevant standards no unfavourable effect of the distillation augmenting techniques applied was noted. Therefore, it should be expected that the studied distillates of volatile fractions will have the desired biological activity.

Resultados: N/A

Conclusão: N/A

Antimicrobial action of essential oil vapours and negative air ions against Pseudomonas fluorescens.

Autores: A K Tyagi, A Malik | Ano: 2010 | Database: pubmed

Resumo: The aim of this study was to investigate the antibacterial activity of essential oil (in liquid as well as in vapour phase) and negative air ions (NAI) against Pseudomonas fluorescens. The combined effect of NAI with essential oil vapour was also investigated to determine kill time and morphological changes in bacterial cells. The MIC of Cymbopogon citratus (0.567 mg/ml), Mentha arvensis (0.567 mg/ml), Mentha piperita (1.125 mg/ml) and Eucalyptus globulus (2.25 mg/ml) was studied via the agar dilution method. To estimate the antibacterial activity of essential oils in the vapour phase, agar plates inoculated with P. fluorescens were incubated with various concentrations of each essential oil vapour and zone of inhibition was recorded. Further, in order to assess the kill time, P. fluorescens inoculated agar plates were exposed to selected bactericidal essential oil vapour and NAI, separately, in an air-tight chamber. A continuous decrease in bacterial count was observed over time. A significant enhancement in the bactericidal action was observed by exposure to the combination of essential oil vapour and NAI as compared to their individual action. Scanning electron microscopy was used to study the alteration in morphology of P. fluorescens cells after exposure to C. citratus oil vapour, NAI, and combination of C. citratus oil vapour and NAI. Maximum morphological deformation was found due to the combined effect of C. citratus oil vapour and NAI. This study demonstrates that the use of essential oils in the vapour phase is more advantageous than the liquid phase. Further the antibacterial effect of the essential oil vapours can be significantly enhanced by the addition of NAI. The work described here offers a novel and efficient approach for control of bacterial contamination that could be applied for food stabilization practices.

Resultados: N/A

Conclusão: N/A

Mentha piperita as a promising feed additive used to protect liver, bone, and meat of Japanese quail against aflatoxin B1.

Autores: Ladan Masouri, Farzad Bagherzadeh-Kasmani, Mehran Mehri, Mohammad Rokouei, Babak Masouri | Ano: 2022 | Database: pubmed

Resumo: The present study was conducted to evaluate aflatoxin B1 (AFB1) detoxification effects of some medicinal plants under both in vitro and in vivo. The in vitro experiment was performed with 25 treatments in 5 replications. The samples of medicinal plants were incubated with AFB1 for 72 h, and the toxin residual in the supernatant was determined. The highest aflatoxin elimination was found to be related to peppermint (81%). Thereafter, in vivo experiment was conducted to investigate the effects of peppermint dried leaves, essential oil, and menthol on liver, bone, and meat, as well as the performance of growing Japanese quail fed diet contaminated with AFB1. A total of 640 7-day-old Japanese quails were assigned using a completely randomized design as 2  4 factorial arrangement with two levels of AFB1 (including 0 and 2.5 mg/kg diet) and four treatments (including no additive; peppermint powder, 20 g/kg; peppermint essential oil, 800 mg/kg; and menthol powder, 400 mg/kg). Those birds fed AFB1-contaminated diet with no additives showed the worst liver health status by considering super oxide dismutase (P = 0.0399), glutathione peroxidase (P = 0.0139), alanine aminotransferase (P < 0.0001), and aspartate aminotransferase levels (P = 0.0512). However, the supplementation of AFB1 contaminated diet with additives improved their liver health status. Menthol receiving birds showed the highest tibia strength, while the birds fed with AFB1-contaminated diet with no additives had the weakest bone strength (P < 0.0001). A significant increase was also observed in malondialdehyde level of meat by dietary inclusion of AFB1, which was well-repressed by the dietary supplementation of peppermint essential oil and menthol (P = 0.0075). Body weight gain dramatically decreased by adding AFB1 to the diet, which was recovered with the dietary supplementation of additives (P = 0.0585). According to the results of the current study, peppermint and its derivatives can be used to suppress aflatoxin effects on the liver, bone, and meat quality and to improve the performance of Japanese quails.

Resultados: N/A

Conclusão: N/A

Effects of essential oils on native and recombinant acetylcholinesterases of Rhipicephalus microplus.

Autores: Everton Gomes Guimarães Dos Santos, Wallyson André Dos Santos Bezerra, Kevin B Temeyer, Adalberto A Pérez de León, Livio Martins Costa-Junior, Alexandra Martins Dos Santos Soares | Ano: 2021 | Database: pubmed

Resumo: This study reports the action of essential oils (EO) from five plants on the activity of native and recombinant acetylcholinesterases (AChE) from Rhipicephalus microplus. Enzyme activity of native susceptible AChE extract (S.AChE), native resistant AChE extract (R.AChE), and recombinant enzyme (rBmAChE1) was determined. An acetylcholinesterase inhibition test was used to verify the effect of the EO on enzyme activity. EO from Eucalyptus globulus, Citrus aurantifolia, Citrus aurantium var.dulcis inhibited the activity of S.AChE and R.AChE. Oils from the two Citrus species inhibited S.AChE and R.AChE in a similar way while showing greater inhibition on R.AChE. The oil from E. globulus inhibited native AChE, but no difference was observed between the S.AChE and R.AChE; however, 71% inhibition for the rBmAChE1 was recorded. Mentha piperita oil also inhibited S.AChE and R.AChE, but there was significant inhibition at the highest concentration tested. Cymbopogon winterianus oil did not inhibit AChE. Further studies are warranted with the oils from the two Citrus species that inhibited R.AChE because of the problem with R. microplus resistant to organophosphates, which target AChE. C. winterianus oil can be used against R. microplus populations that are resistant to organophosphates because its acaricidal properties act by mechanism(s) other than AChE inhibition.

Resultados: N/A

Conclusão: N/A

Agrobiological Interactions of Essential Oils of Two Menthol Mints: Mentha piperita and Mentha arvensis.

Autores: Danuta Kalemba, Agnieszka Synowiec | Ano: 2019 | Database: pubmed

Resumo: This review article discusses the active constituents and potential of two menthol mint oils, Mentha piperita (MPEO) and Mentha arvensis (MAEO), as natural sources for botanical pesticides. The biological activities of these menthol mint oils, which can be useful in agriculture, have been broadly researched, especially toward phytotoxic microorganisms. To a lesser extent, the insecticidal and herbicidal activities of mint EOs have also been studied. It is apparent that the prospect of using menthol mint oils in agriculture is increasing in popularity. A number of investigations showed that the in vitro efficacy of MPEO and MAEO, as well as that of their main constituent, menthol, is pronounced. The results of in vitro research are useful for choosing EOs for further investigations. However, it is clear that in situ experiments are crucial and should be more extensively developed. At the same time, known techniques are to be applied to this area and new methods should be worked out, aiming at the improvement of EOs' pesticidal efficacy and cost-effectiveness, for future implementation in agricultural pest control.

Resultados: N/A

Conclusão: N/A

Comparative glandular trichome transcriptome-based gene characterization reveals reasons for differential (-)-menthol biosynthesis in Mentha species.

Autores: Md Qussen Akhtar, Nida Qamar, Pallavi Yadav, Pallavi Kulkarni, Ajay Kumar, Ajit Kumar Shasany | Ano: 2017 | Database: pubmed

Resumo: The genes involved in menthol biosynthesis are reported earlier in Mentha  piperita. But the information on these genes is not available in Mentha arvensis. To bridge the gap in knowledge on differential biosynthesis of monoterpenes leading to compositional variation in the essential oil of these species, a comparative transcriptome analysis of the glandular trichome (GT) was carried out. In addition to the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathway genes, about 210 and 196 different terpene synthases (TPSs) transcripts were identified from annotation in M. arvensis and M.  piperita, respectively, and correlated to several monoterpenes present in the essential oil. Six isoforms of (-)-menthol dehydrogenases (MD), the last enzyme of the menthol biosynthetic pathway, were identified, cloned and characterized from the transcriptome data (three from each species). Varied expression levels and differential enzyme kinetics of these isoforms indicated the nature and composition of the product, as these isoforms generate both (-)-menthol and (+)-neomenthol from (-)-menthone and converts (-)-menthol to (-)-menthone in the reverse reaction, and hence together determine the quantity of (-)-menthol in the essential oil in these two species. Several genes for high value minor monoterpenes could also be identified from the transcriptome data.

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Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model.

Autores: Mohammad Modarresi, Mohammad-Reza Farahpour, Behzad Baradaran | Ano: 2019 | Database: pubmed

Resumo: This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25  107 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.

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Physiological indexese macro- and micronutrients in plant tissue and essential oil of Mentha piperita L. grown in nutrient solution with variation in N, P, K and Mg levels/ Índices fisiológicos, macro e micronutrientes no tecido vegetal e óleo essencial de Mentha piperita L. cultivada em solução nutritiva com variação de N, P, K e Mg.

Autores: David, E.F.S.; Mischan, M.M.; Marques, M.O.M.; Boaro, C.S.F. | Ano: 2014 | Database: LILACS

Resumo: Mentha piperita L. is an aromatic and medicinal species of the family Lamiaceae, known as mint or peppermint, and its leaves and branches produce essential oil rich in menthol. This study aimed to evaluate physiological indexes, macro- and micronutrients inthe shootsand essential oil of Mentha piperita L. grown in nutrient solution number 2 of Hoagland and Arnon (1950) with different N, P, K and Mg levels. Shoot length, dry mass of the different organs, total dry mass, leaf area, essential oil yield and composition, and macronutrient (N, P, K, Mg, Ca, S) and micronutrient (Mn, Cu, Fe, Zn) contents in the shoot were evaluated. Plants treated with 65%N/50%P/25%K/100%Mg had a tendency towards longer shoot, greaterroot and leaf blade dry masses, higher essential oil yield, higher menthol levels and lower menthone levels. The results showed that Mentha can be grown in nutrient solution by reducing 65% N, 50% P, 25% K and 100% Mg. This solution had better development compared to the other tested treatments. Therefore,we recommendMentha piperita L. to be grown with such nutrient levels.

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Salt Stress Affects Plastid Ultrastructure and Photosynthetic Activity but Not the Essential Oil Composition in Spearmint (Mentha spicata L. var. crispa "Moroccan").

Autores: Roumaissa Ounoki, Ferenc Ágh, Richard Hembrom, Renáta Ünnep, Bernadett Szögi-Tatár, Andrea Böszörményi, Katalin Solymosi | Ano: 2021 | Database: pubmed

Resumo: High levels of soil salinity affect plant growth, reproduction, water and ion uptake, and plant metabolism in a complex manner. In this work, the effect of salt stress on vegetative growth, photosynthetic activity, and chloroplast ultrastructure of spearmint (Mentha spicata L. var. crispa "Moroccan") was investigated. After 2 weeks of low concentration treatments (5, 25, and 50 mM NaCl) of freshly cut shoots, we observed that the stem-derived adventitious root formation, which is a major mean for vegetative reproduction among mints, was completely inhibited at 50 mM NaCl concentration. One-week-long, high concentration (150 mM NaCl) salt stress, and isosmotic polyethylene glycol (PEG) 6000 treatments were compared in intact (rooted) plants and freshly cut, i.e., rootless shoots. Our data showed that roots have an important role in mitigating the deleterious effects of both the osmotic (PEG treatment) and specific ionic components of high salinity stress. At 50 mM NaCl or above, the ionic component of salt stress caused strong and irreversible physiological alterations. The effects include a decrease in relative water content, the maximal and actual quantum efficiency of photosystem II, relative chlorophyll content, as well as disorganization of the native chlorophyll-protein complexes as revealed by 77 K fluorescence spectroscopy. In addition, important ultrastructural damage was observed by transmission electron microscopy such as the swelling of the thylakoid lumen at 50 mM NaCl treatment. Interestingly, in almost fully dry leaf regions and leaves, granum structure was relatively well retained, however, their disorganization occurred in leaf chloroplasts of rooted spearmint treated with 150 mM NaCl. This loss of granum regularity was also confirmed in the leaves of these plants using small-angle neutron scattering measurements of intact leaves of 150 mM NaCl-stressed rooted plants. At the same time, solid-phase microextraction of spearmint leaves followed by gas chromatography and mass spectrometry (GC/MS) analyses revealed that the essential oil composition of spearmint was unaffected by the treatments applied in this work. Taken together, the used spearmint cultivar tolerates low salinity levels. However, at 50 mM NaCl concentration and above, the ionic components of the stress strongly inhibit adventitious root formation and thus their clonal propagation, and severely damage the photosynthetic apparatus.

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Yield, content, and composition of peppermint and spearmints as a function of harvesting time and drying.

Autores: Valtcho D Zheljazkov, Charles L Cantrell, Tess Astatkie, Alex Hristov | Ano: 2010 | Database: pubmed

Resumo: Peppermint ( Mentha piperita L.) and spearmints ('Scotch' spearmint, M. gracilis Sole, and 'Native' spearmint, Mentha spicata L.) are widely grown essential oil crops in more northern latitudes; however, there is limited information on how harvest time and drying influence peppermint and spearmint yield, oil composition, and bioactivity, when grown south of the 41st parallel. In this 2-year study, the effects of harvest time and drying on the yield, oil composition, and bioactivity of peppermint ('Black Mitcham' and 'B90-9'), 'Scotch' spearmint, and 'Native' spearmint were evaluated. Peppermint oil from the dried material had higher menthol and eucalyptol concentrations. Menthone in both peppermint cultivars decreased from harvest 1 (late June) to harvest 5 (late August) or 6 (early September), whereas menthol increased. (-)-Carvone in spearmints accumulated early, before flowering, allowing for early harvest. Oil yields from the dried spearmint biomass reached the maximum at harvest 3 (mid-July). The essential oil compositions of the four mint genotypes were similar to that of 11 commercially available oils, suggesting that these genotypes can be grown in the hot, humid environment of the southeastern United States. The antioxidant activities (ORAC(oil) values) of the essential oils were 4372, 1713, 1107, and 471 μmol of TE L(-1) for 'Scotch' spearmint, 'Native' spearmint, peppermint, and Japanese cornmint ( Mentha canadensis ), respectively. The oils of the four mint genotypes did not affect ruminal fermentation in vivo, and did not exhibit antimicrobial, antileishmanial, or antimalarial activity at levels that would warrant bioassay-directed fractionation in a drug-discovery screening program. Specifically, the oils did not show greater than 50% growth inhibition against Leishmania donovani , Plasmodium falciparum clones D6 and W2, Candida albicans , Escherichia coli , Pseudomonas aeruginosa , Cryptococcus neoformans , Mycobacterium intracellulare , or Aspergillus fumigates at 50 μg mL(-1).

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Comparative Chemical Analysis of Mentha piperita and M. spicata and a Fast Assessment of Commercial Peppermint Teas.

Autores: Mihaela Buleandra, Eliza Oprea, Dana Elena Popa, Iulia Gabriela David, Zenovia Moldovan, Iuliana Mihai, Irinel Adriana Badea | Ano: 2016 | Database: pubmed

Resumo: The hydrodistilled essential oils and volatile compounds (by static headspaces technique) of Mentha piperita L. and M spicata L. were characterized by GC-MS. Headspace analysis of Mentha piperita revealed the existence of menthone (25.4%), 1,8-cineole (17.7%) and menthol (12.1%) as the main components, while the essential oil contained high amounts of menthol (46.8%) and menthone (25.6%). By contrast, headspace analysis of M. spicata showed a high content of limonene (37.0%) together with carvone (13.0%), β-pinene (10.4%) and α-pinene (9.8%), while the essential oil was reach in carvone (51.7%), dihydrocarveol (11.5%) and cis-dihydrocarvone (9.1%). Eleven samples of peppermint tea available on the Romanian market were analysed by headspace GC-MS. The volatile profile of the tea samples was compared with that of Mentha piperita L. and certain differences were emphasized and discussed.

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Essential Oils, Part V: Peppermint Oil, Lavender Oil, and Lemongrass Oil.

Autores: Anton de Groot, Erich Schmidt | Ano: 2016 | Database: pubmed

Resumo: Some aspects of peppermint oil, lavender oil, and lemongrass oil are discussed including their botanical origin, uses of the plants and the oils, chemical composition, contact allergy to and allergic contact dermatitis from these essential oils, and causative allergenic ingredients.

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Rapid Screening of Mentha spicata Essential Oil and L-Menthol in Mentha piperita Essential Oil by ATR-FTIR Spectroscopy Coupled with Multivariate Analyses.

Autores: Osman Taylan, Nur Cebi, Osman Sagdic | Ano: 2021 | Database: pubmed

Resumo: Mentha piperita essential oil (EO) has high economic importance because of its wide usage area and health-beneficial properties. Besides health-beneficial properties, Mentha piperita EO has great importance in the flavor and food industries because of its unique sensory and quality properties. High-valued essential oils are prone to being adulterated with economic motivations. This kind of adulteration deteriorates the quality of authentic essential oil, injures the consumers, and causes negative effects on the whole supply chain from producer to the consumer. The current research used fast, economic, robust, reliable, and effective ATR-FTIR spectroscopy coupled chemometrics of hierarchical cluster analysis(HCA), principal component analysis (PCA), partial least squares regression (PLSR) and principal component regression (PCR) for monitoring of Mentha spicata EO and L-menthol adulteration in Mentha piperita EOs. Adulterant contents (Mentha spicata and L-menthol) were successfully calculated using PLSR and PCR models. Standard error of the cross-validation SECV values changed between 0.06 and 2.14. Additionally, bias and press values showed alteration between 0.06 and1.43 and 0.03 and 41.15, respectively. Authentic Mentha piperita was successfully distinguished from adulterated samples, Mentha spicata and L-menthol, by HCA and PCA analysis. The results showed that attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, coupled with chemometrics could be effectively used for monitoring various adulterants in essential oils.

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Rapid Screening of Mentha spicata Essential Oil and L-Menthol in Mentha piperita Essential Oil by ATR-FTIR Spectroscopy Coupled with Multivariate Analyses.

Autores: Osman Taylan, Nur Cebi, Osman Sagdic | Ano: 2021 | Database: pubmed

Resumo: Mentha piperita essential oil (EO) has high economic importance because of its wide usage area and health-beneficial properties. Besides health-beneficial properties, Mentha piperita EO has great importance in the flavor and food industries because of its unique sensory and quality properties. High-valued essential oils are prone to being adulterated with economic motivations. This kind of adulteration deteriorates the quality of authentic essential oil, injures the consumers, and causes negative effects on the whole supply chain from producer to the consumer. The current research used fast, economic, robust, reliable, and effective ATR-FTIR spectroscopy coupled chemometrics of hierarchical cluster analysis(HCA), principal component analysis (PCA), partial least squares regression (PLSR) and principal component regression (PCR) for monitoring of Mentha spicata EO and L-menthol adulteration in Mentha piperita EOs. Adulterant contents (Mentha spicata and L-menthol) were successfully calculated using PLSR and PCR models. Standard error of the cross-validation SECV values changed between 0.06 and 2.14. Additionally, bias and press values showed alteration between 0.06 and1.43 and 0.03 and 41.15, respectively. Authentic Mentha piperita was successfully distinguished from adulterated samples, Mentha spicata and L-menthol, by HCA and PCA analysis. The results showed that attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, coupled with chemometrics could be effectively used for monitoring various adulterants in essential oils.

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A Comparative Analysis of the Chemical Composition, Anti-Inflammatory, and Antinociceptive Effects of the Essential Oils from Three Species of Mentha Cultivated in Romania.

Autores: Cristina Mogosan, Oliviu Vostinaru, Radu Oprean, Codruta Heghes, Lorena Filip, Georgeta Balica, Radu Ioan Moldovan | Ano: 2017 | Database: pubmed

Resumo: This work was aimed at correlating the chemotype of three Mentha species cultivated in Romania with an in vivo study of the anti-inflammatory and antinociceptive effects of essential oils. The selected species were Mentha piperita L. var. pallescens (white peppermint), Mentha spicata L. subsp. crispata (spearmint), and Mentha suaveolens Ehrh. (pineapple mint). Qualitative and quantitative analysis of the essential oils isolated from the selected Mentha species was performed by gas chromatography coupled with mass spectrometry (GC-MS). The anti-inflammatory activity of the essential oils was determined by the rat paw edema test induced by λ-carrageenan. The antinociceptive effect of the essential oils was evaluated by the writhing test in mice, using 1% (v/v) acetic acid solution administered intraperitonealy and by the hot plate test in mice. The results showed a menthol chemotype for M. piperita pallescens, a carvone chemotype for M. spicata, and a piperitenone oxide chemotype for M. suaveolens. The essential oil from M. spicata L. (EOMSP) produced statistically significant and dose-dependent anti-inflammatory and antinociceptive effects.

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A Comparative Analysis of the Chemical Composition, Anti-Inflammatory, and Antinociceptive Effects of the Essential Oils from Three Species of Mentha Cultivated in Romania.

Autores: Cristina Mogosan, Oliviu Vostinaru, Radu Oprean, Codruta Heghes, Lorena Filip, Georgeta Balica, Radu Ioan Moldovan | Ano: 2017 | Database: pubmed

Resumo: This work was aimed at correlating the chemotype of three Mentha species cultivated in Romania with an in vivo study of the anti-inflammatory and antinociceptive effects of essential oils. The selected species were Mentha piperita L. var. pallescens (white peppermint), Mentha spicata L. subsp. crispata (spearmint), and Mentha suaveolens Ehrh. (pineapple mint). Qualitative and quantitative analysis of the essential oils isolated from the selected Mentha species was performed by gas chromatography coupled with mass spectrometry (GC-MS). The anti-inflammatory activity of the essential oils was determined by the rat paw edema test induced by λ-carrageenan. The antinociceptive effect of the essential oils was evaluated by the writhing test in mice, using 1% (v/v) acetic acid solution administered intraperitonealy and by the hot plate test in mice. The results showed a menthol chemotype for M. piperita pallescens, a carvone chemotype for M. spicata, and a piperitenone oxide chemotype for M. suaveolens. The essential oil from M. spicata L. (EOMSP) produced statistically significant and dose-dependent anti-inflammatory and antinociceptive effects.

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Increased seizure latency and decreased severity of pentylenetetrazol-induced seizures in mice after essential oil administration.

Autores: Eleni Koutroumanidou, Athanasios Kimbaris, Alexandros Kortsaris, Eugenia Bezirtzoglou, Moschos Polissiou, Konstantinos Charalabopoulos, Olga Pagonopoulou | Ano: 2013 | Database: pubmed

Resumo: The effect of pretreatment with essential oils (EOs) from eight aromatic plants on the seizure latency and severity of pentylenetetrazol- (PTZ-) induced seizures in mice was evaluated. Weight-dependent doses of Rosmarinus officinalis, Ocimum basilicum, Mentha spicata, Mentha pulegium, Lavandula angustifolia, Mentha piperita, Origanum dictamnus, and Origanum vulgare, isolated from the respective aromatic plants from NE Greece, were administered 60 minutes prior to intraperitoneal (i.p.) injection of a lethal dose of PTZ to eight respective groups of Balb-c mice. Control group received only one i.p. PTZ injection. Motor and behavioral activity of the animals after EOs administration, development of tonic-clonic seizures, seizure latency and severity, and percentage of survival after PTZ administration were determined for each group. All groups of mice treated with the EOs showed reduced activity and stability after the administration of the oil, except for those treated with O. vulgare (100% mortality after the administration of the oil). After PTZ administration, mice from the different groups showed increased latency and reduced severity of seizures (ranging from simple twitches to complete seizures). Mice who had received M. piperita demonstrated no seizures and 100% survival. The different drastic component and its concentration could account for the diversity of anticonvulsant effects.

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Mentha piperita Oil Exerts an Antiepileptic Effect in Pilocarpine and Pentylenetetrazol-Induced Seizures in Mice.

Autores: Waleed K Abdulsahib, Sarmed H Kathem, Mohanad Y Al-Radeef, Layth S Jasim | Ano: 2022 | Database: pubmed

Resumo: Epilepsy is a progressive, chronic neurological disorder characterized by recurrent seizures. Peppermint (Mentha piperita L.) (MP) is one of the most commonly ingested herbal teas or tisanes with a single component.

Resultados: MPO significantly (P < 0.05) increases the first convulsion's latency time and the death's latency time. Moreover, the essential oil significantly decreases the number of convulsions and reduces the mortality rate compared to the negative control group.

Conclusão: MPO at 3.2 and 6.4 ml/kg doses can reduce the percentage and the number of convulsions and increase the latency time of both the first convulsion and death so that it can be used as a supplement in the treatment of epilepsy.

Antispasmodic effect of Mentha piperita essential oil on tracheal smooth muscle of rats.

Autores: Albertina Antonielly Sydney de Sousa, Pedro Marcos Gomes Soares, Arisa Nara Saldanha de Almeida, Alana Rufino Maia, Emmanuel Prata de Souza, Ana Maria Sampaio Assreuy | Ano: 2010 | Database: pubmed

Resumo: Mentha piperita is a plant popularly known in Brazil as "hortelã-pimenta" whose essential oil is used in folk medicine for its anti-inflammatory, antispasmodic, expectorant actions and anti-congestive. Here, it was investigated the effect of Mentha piperita essential oil (peppermint oil) in rat tracheal rings along with its mechanism of action.

Resultados: Peppermint oil [100 and 300 microg/ml] inhibited the contractions induced by carbachol, which was reversed by indomethacin, L-N-metyl-nitro-arginine and hexamethonium, but not by tetraethylammonium. These data suggest the participation of prostaglandin E(2), nitric oxide and autonomic ganglions in the peppermint oil relaxant effect and may be correlated with its popular use in respiratory diseases.

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Synergistic mixtures of chitosan and Mentha piperita L. essential oil to inhibit Colletotrichum species and anthracnose development in mango cultivar Tommy Atkins.

Autores: Kataryne Árabe Rimá de Oliveira, Lúcia Raquel Ramos Berger, Samara Amorim de Araújo, Marcos Paz Saraiva Câmara, Evandro Leite de Souza | Ano: 2017 | Database: pubmed

Resumo: This study assessed the efficacy of chitosan (CHI) and Mentha piperita L. essential oil (MPEO) alone or in combination to control the mycelial growth of five different Colletotrichum species, C. asianum, C. dianesei, C. fructicola, C. tropicale and C. karstii, identified as potential anthracnose-causing agents in mango (Mangifera indica L.). The efficacy of coatings of CHI and MPEO mixtures in controlling the development of anthracnose in mango cultivar Tommy Atkins was evaluated. CHI (2.5, 5, 7.5 and 10 mg/mL) and MPEO (0.3, 0.6, 1.25, 2.5 and 5 μL/mL) alone effectively inhibited mycelial growth of all tested Colletotrichum strains in synthetic media. Mixtures of CHI (5 or 7.5 mg/mL) and MPEO (0.3, 0.6 or 1.25 μL/mL) strongly inhibited mycelial growth and showed additive or synergistic inhibitory effects on the tested Colletotrichum strains based on the Abbott index. The application of coatings of CHI (5 or 7.5 mg/mL) and MPEO (0.6 or 1.25 μL/mL) mixtures that presented synergistic interactions decreased anthracnose lesion severity in mango artificially contaminated with either of the tested Colletotrichum strains over 15 days of storage at 25 °C. The anthracnose lesion severity in mango coated with the mixtures of CHI and MPEO was similar or lower than those observed in mango treated with the synthetic fungicides thiophanate-methyl (10 μg a.i./mL) and difenoconazole (0.5 μg a.i./mL). The application of coatings containing low doses of CHI and MPEO may be an effective alternative for controlling the postharvest development of anthracnose in mango cultivar Tommy Atkins.

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Biological control of Citrus brown spot pathogen, "Alternaria alternata" by different essential oils.

Autores: Taycir Grati Affes, Synda Chenenaoui, Hassen Zemni, Majdi Hammami, Sarra Bachkouel, Wissem Aidi Wannes, Bouzid Nasraoui, Moufida Saidani Tounsi, Salma Lasram | Ano: 2022 | Database: pubmed

Resumo: The antifungal effects of laurel, myrtle and peppermint essential oils and their combinations were investigated in vitro on two strains of Alternaria alternata mycelial growth and in vivo on detached Citrus leaf disease incidence. Myrtle essential oil was rich in α-pinene and 1,8-cineole while peppermint essential oil in menthol and menthone. 1,8-Cineole was the main component of the essential oils from laurel, laurel + myrtle and peppermint + laurel. The combined peppermint and myrtle essential oil was characterized by the predominance of menthol and 1,8-cineole. All tested essential oils, incorporated in potato-dextrose agar, inhibited A. alternata mycelial growth and had a fungistatic effect at concentration 3 mg/mL of medium. A great synergism was detected between peppermint and laurel essential oils against the two strains of A. alternata. The combined laurel and peppermint essential oil reduced mycelial growth rates of inoculated detached leaves at concentration above 1.5 mg/mL.

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Coatings comprising chitosan and Mentha piperita L. or Mentha villosa Huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit.

Autores: Ingrid Conceição Dantas Guerra, Priscila Dinah Lima de Oliveira, Alline Lima de Souza Pontes, Ana Sílvia Suassuna Carneiro Lúcio, Josean Fechine Tavares, José Maria Barbosa-Filho, Marta Suely Madruga, Evandro Leite de Souza | Ano: 2015 | Database: pubmed

Resumo: In this study, we evaluated the efficacy of coatings comprising shrimp chitosan (CHI) and Mentha piperita L. (MPEO) or Mentha villosa Huds (MVEO) essential oils to control mold infections caused by Aspergillus niger, Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer in cherry tomato fruits (Solanum lycopersicum L.) during storage at room temperature (25°C for 12 days) and low temperature (12°C for 24 days). The effects of the coatings on the physicochemical and sensory characteristics of cherry tomato fruits during storage were also assessed. The minimum inhibitory concentration (MIC) of CHI against all test fungi was 8 mg/mL, whereas the MIC for both MPEO and MVEO was 5 μL/mL. Combinations of CHI at 4 mg/mL and MPEO or MVEO at 2.5 or 1.25 μL/mL strongly inhibited the mycelial growth and spore germination of target fungi. The coatings comprising CHI and MPEO or CHI and MVEO at the different tested concentrations delayed the growth of decay-causing fungi in artificially contaminated tomato fruit during storage at either room temperature or low temperature. The assayed coatings preserved the quality of cherry tomato fruit during storage, in terms of physicochemical and sensory attributes. These results indicate that coatings comprising CHI and MPEO or CHI and MVEO represent promising postharvest treatments to prevent common postharvest mold infections in cherry tomato fruit during storage without affecting the quality of the fruit.

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The Effects of Mentha piperita Essential Oil on C. albicans Growth, Transition, Biofilm Formation, and the Expression of Secreted Aspartyl Proteinases Genes.

Autores: Chahrazed Benzaid, Amine Belmadani, Ryad Djeribi, Mahmoud Rouabhia | Ano: 2019 | Database: pubmed

Resumo: The rise in resistance and changes in the spectrum of Candida infections have generated enormous interest in developing new antifungal drugs using natural molecules such as plant essential oils (EOs). Antimicrobial activity against foodborne pathogenic and spoilage microorganisms has been reported for EOs. The goal of this study was to assess the effect of Mentha piperita essential oil (EO) on C. albicans growth, transition (change from blastospore to hyphae forms), and biofilm formation as well as on the expression of certain virulent genes. We show that whole EO and its vapor attenuated the yeast's growth, compared to that in the control. The effect of the EO was comparable to that of amphotericin-B (AmB). The EO and its vapor significantly decreased the morphological changes of C. albicans, reduced biofilm formation, and disrupted mature C. albicans biofilms. The effect produced by whole EO on biofilm formation/disruption was notably comparable to that observed with AmB. Exposure of C. albicans to EO and its vapor downregulated the expression of various genes, such as secreted aspartyl proteinases (SAP 1, 2, 3, 9, 10) and hyphal wall protein 1 (HWP1). Altogether, these results provide new insight into the efficacy of Mentha piperita EO against C. albicans and suggest the potential of Mentha piperita EO for use as an antifungal therapy in multiple applications.

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Volatile Terpenes and Brain Function: Investigation of the Cognitive and Mood Effects of Mentha Piperita L. Essential Oil with In Vitro Properties Relevant to Central Nervous System Function.

Autores: David Kennedy, Edward Okello, Paul Chazot, Melanie-Jayne Howes, Samuel Ohiomokhare, Philippa Jackson, Crystal Haskell-Ramsay, Julie Khan, Joanne Forster, Emma Wightman | Ano: 2018 | Database: pubmed

Resumo: Extracts of several members of the monoterpene-rich Lamiaceae sub-family Nepetoideae, including those from the Salvia (sage), Melissa (Lemon balm) and Rosmarinus (rosemary) genera, evince cognitive and mood effects in humans that are potentially related to their effects on cholinergic and GABAergic neurotransmission. To date, despite promising in vitro properties, the cognitive and mood effects of the closely related Mentha spicata (spearmint) and Mentha piperita (peppermint) remain unexplored. This study therefore assessed the human cognitive/mood effects of the M. spicata/piperita essential oil with the most promising, brain-relevant in vitro properties according to pre-trial in vitro screening. Design: Organic spearmint and peppermint (Mentha spicata/piperita) essential oils were pre-screened for neurotransmitter receptor binding and acetylcholinesterase (AChE) inhibition. In a double-blind, placebo-controlled, balanced cross-over study, 24 participants (mean age 25.2 years) consumed single doses of encapsulated placebo and 50 µl and 100 µl of the most promising essential oil (peppermint with nicotinic/GABAA receptor binding and AChE inhibitory properties, that increased calcium influx in a CAD cell neuronal model). Psychological functioning was assessed with mood scales and a range of standardised, cognitively demanding tasks pre-dose and at 1, 3 and 6 h post-dose. Results: The highest (100 µL) dose of essential oil improved performance on the cognitively demanding Rapid Visual Information Processing task (RVIP) at 1 h and 3 h post-dose and both doses attenuated fatigue and improved performance of the Serial 3 s subtraction task at 3 h post-dose. Conclusion: Peppermint (Mentha piperita) essential oil with high levels of menthol/menthone and characteristic in vitro cholinergic inhibitory, calcium regulatory and GABAA/nicotinic receptor binding properties, beneficially modulated performance on demanding cognitive tasks and attenuated the increase in mental fatigue associated with extended cognitive task performance in healthy adults. Future investigations should consider investigating higher doses.

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Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria.

Autores: Gulsun Akdemir Evrendilek | Ano: 2015 | Database: pubmed

Resumo: In this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (Pimpinella anisum), bay leaves (Laurus nobilis), cinnamon bark (Cinnamomum verum), clove (Eugenia caryophyllata), fennel (Foeniculum vulgare), hop (Humulus lupulus), Istanbul oregano (Origanum vulgare subsp. hirtum), Izmir oregano (Origanum onites), mint (Mentha piperita), myrtus (Myrtus communis), orange peel (Citrus sinensis), sage (Salvia officinalis), thyme (Thymbra spicata), and Turkish oregano (Origanum minutiflorum)--were related to inhibition of 10 bacteria through multiple linear or non-linear (M(N)LR) models-four Gram-positive bacteria of Listeria innocua, coagulase-negative staphylococci, Staphylococcus aureus, and Bacillus subtilis, and six Gram-negative bacteria of Yersinia enterocolitica, Salmonella Enteritidis, Salmonella Typhimurium, Proteus mirabilis, Escherichia coli O157:H7, and Klebsiella oxytoca. A total of 65 compounds with different antioxidant capacity, phenolic substance content and antibacterial properties were detected with 14 plant essential oils. The best-fit M(N)LR models indicated that relative to anise essential oil, the essential oils of oreganos, cinnamon, and thyme had consistently high inhibitory effects, while orange peel essential oil had consistently a low inhibitory effect. Regression analysis indicated that beta-bisabolene (Turkish and Istanbul oreganos), and terpinolene (thyme) were found to be the most inhibitory compounds regardless of the bacteria type tested.

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Inhalation of Essential Oil from Mentha piperita Ameliorates PM10-Exposed Asthma by Targeting IL-6/JAK2/STAT3 Pathway Based on a Network Pharmacological Analysis.

Autores: Mi Hye Kim, Sang Jun Park, Woong Mo Yang | Ano: 2020 | Database: pubmed

Resumo: Fine particulate matter (PM) exposure exhibits a crucial risk factor to exacerbate airway epithelial remodeling, fibrosis, and pulmonary destruction in asthma. Based on the use of essential oils from aromatic plants on pain relief and anti-inflammatory properties, we investigated the inhibitory effects of essential oil derived from the Mentha species (MEO) against asthma exposed to PM10. The MEO (0.1 v/v %) was aerosolized by a nebulizer to ovalbumin and PM10-induced asthmatic mice. Histological changes were confirmed in the lung tissues. To define the mode of action of the MEO on asthma, a protein-protein interaction network was constructed using menthol and menthone as the major components of the MEO. Cytokine expression and the JAK2/STAT3 signaling pathway were analyzed in lung epithelial A549 cells co-treated with MEO and PM10. Inhalation of MEO by nebulization inhibited respiratory epithelium hyperplasia, collagen deposition, and goblet cell activation in asthmatic mice. Through a network pharmacological analysis, cytokine-cytokine receptor interaction and JAK/STAT was expected to be underlying mechanisms of MEO on asthma. Treatment with MEO significantly reduced the IL-6 levels with a decrease in pro-inflammatory and T helper 2-specific cytokines. PM10-induced phosphorylation of JAK2 and STAT3 was significantly decreased by MEO. Collectively, MEO may have an inhibitory effect on asthma under the condition of PM10 exposure through the IL-6/JAK2/STAT3 signaling pathway.

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Conclusão: N/A

Inhalation of Essential Oil from Mentha piperita Ameliorates PM10-Exposed Asthma by Targeting IL-6/JAK2/STAT3 Pathway Based on a Network Pharmacological Analysis.

Autores: Mi Hye Kim, Sang Jun Park, Woong Mo Yang | Ano: 2020 | Database: pubmed

Resumo: Fine particulate matter (PM) exposure exhibits a crucial risk factor to exacerbate airway epithelial remodeling, fibrosis, and pulmonary destruction in asthma. Based on the use of essential oils from aromatic plants on pain relief and anti-inflammatory properties, we investigated the inhibitory effects of essential oil derived from the Mentha species (MEO) against asthma exposed to PM10. The MEO (0.1 v/v %) was aerosolized by a nebulizer to ovalbumin and PM10-induced asthmatic mice. Histological changes were confirmed in the lung tissues. To define the mode of action of the MEO on asthma, a protein-protein interaction network was constructed using menthol and menthone as the major components of the MEO. Cytokine expression and the JAK2/STAT3 signaling pathway were analyzed in lung epithelial A549 cells co-treated with MEO and PM10. Inhalation of MEO by nebulization inhibited respiratory epithelium hyperplasia, collagen deposition, and goblet cell activation in asthmatic mice. Through a network pharmacological analysis, cytokine-cytokine receptor interaction and JAK/STAT was expected to be underlying mechanisms of MEO on asthma. Treatment with MEO significantly reduced the IL-6 levels with a decrease in pro-inflammatory and T helper 2-specific cytokines. PM10-induced phosphorylation of JAK2 and STAT3 was significantly decreased by MEO. Collectively, MEO may have an inhibitory effect on asthma under the condition of PM10 exposure through the IL-6/JAK2/STAT3 signaling pathway.

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Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats.

Autores: Khaled Bellassoued, Anis Ben Hsouna, Khaled Athmouni, Jos van Pelt, Fatma Makni Ayadi, Tarek Rebai, Abdelfattah Elfeki | Ano: 2018 | Database: pubmed

Resumo: Mentha piperita L. is a flowering plant belonging to the Lamiaceae family. Mentha plants constitute one of the main valuable sources of essential oil used in foods and for medicinal purposes.

Resultados: The in vitro antioxidant activity of MpEO was lower than that of silymarin. Pretreatment of animals with MpEO at a dose of 5 mg/kg did not have a significant effect on ALT, AST, ALP, LDH, γGT, urea or creatinine levels in CCl4-induced stress. Whereas pretreatment with MpEO at doses of 15 and 40 mg/kg prior to CCl4, significantly reduced stress parameters (ALT, AST, ALP, LDH, γGT, urea and creatinine) compared to the CCl4-only group. Moreover, a significant reduction in hepatic and kidney lipid peroxidation (TBARS) and an increase in antioxidant enzymes SOD, CAT and GPx was also observed after treatment with MpEO (40 mg/kg) compared to CCl4-treated rats. Furthermore, pretreatment with MpEO at 40 mg/kg can also markedly ameliorate the histopathological hepatic and kidney lesions induced by administration of CCl4.

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Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats.

Autores: Khaled Bellassoued, Anis Ben Hsouna, Khaled Athmouni, Jos van Pelt, Fatma Makni Ayadi, Tarek Rebai, Abdelfattah Elfeki | Ano: 2018 | Database: pubmed

Resumo: Mentha piperita L. is a flowering plant belonging to the Lamiaceae family. Mentha plants constitute one of the main valuable sources of essential oil used in foods and for medicinal purposes.

Resultados: The in vitro antioxidant activity of MpEO was lower than that of silymarin. Pretreatment of animals with MpEO at a dose of 5 mg/kg did not have a significant effect on ALT, AST, ALP, LDH, γGT, urea or creatinine levels in CCl4-induced stress. Whereas pretreatment with MpEO at doses of 15 and 40 mg/kg prior to CCl4, significantly reduced stress parameters (ALT, AST, ALP, LDH, γGT, urea and creatinine) compared to the CCl4-only group. Moreover, a significant reduction in hepatic and kidney lipid peroxidation (TBARS) and an increase in antioxidant enzymes SOD, CAT and GPx was also observed after treatment with MpEO (40 mg/kg) compared to CCl4-treated rats. Furthermore, pretreatment with MpEO at 40 mg/kg can also markedly ameliorate the histopathological hepatic and kidney lesions induced by administration of CCl4.

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Effects of Olfactory Stimulation with Aroma Oils on Psychophysiological Responses of Female Adults.

Autores: Na-Yoon Choi, Yu-Tong Wu, Sin-Ae Park | Ano: 2022 | Database: pubmed

Resumo: This study investigated the effects of olfactory stimulation with aroma oils on the psychophysiological responses in women. Ten aromatic oils (lavender, rosemary, rose, eucalyptus, jasmine, geranium, chamomile, clary sage, thyme, and peppermint) were used on 23 women aged between 20 and 60 years. They inhaled the scent for 90 s through a glass funnel attached to their lab apron, 10 cm below their nose, while the pump was activated. Electroencephalography, blood pressure, and pulse rate were measured before and during inhalation of the aroma oils. The relative alpha (RA) power spectrums indicating relaxation and resting state of the brain significantly increased when lavender, rosemary, eucalyptus, jasmine, chamomile, clary sage, and thyme oils were inhaled compared to those of before olfactory stimulation. The ratio of alpha to high beta (RAHB), an indicator of brain stability and relaxation, significantly increased when rosemary, jasmine, clary sage, and peppermint oils were inhaled. The relative low beta (RLB) power spectrum, an indicator of brain activity in the absence of stress, significantly increased when stimulated with lavender, rosemary, rose, and geranium scents. Further, systolic blood pressure significantly decreased after introduction of all 10 types of aromatic oils, which indicates stress reduction. Thus, olfactory stimulation with aroma oil had a stabilizing effect on the prefrontal cortex and brain activity and decreased systolic blood pressure.

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Nanogels Containing Foeniculum vulgare Mill. and Mentha piperita L. Essential Oils: Mosquitoes' Repellent Activity and Antibacterial Effect.

Autores: Alireza Sanei-Dehkordi, Abbas Abdollahi, Mojdeh Safari, Farnaz Karami, Ghazal Ghaznavi, Mahmoud Osanloo | Ano: 2022 | Database: pubmed

Resumo: Foeniculum vulgare Mill. and Mentha piperita L. are two common medicinally important plants with a wide range of biological activities such as insecticide and antibacterial effects. In this study, the chemical composition of their essential oils was investigated using GC-MS analysis. After that, their nanoemulsions were prepared; optimum samples with droplet sizes of 74 ± 7 and 136 ± 5 nm were gelified. The viscosity of the prepared nanogels and the successful loading of the essential oil in them were investigated. The efficacy of the nanogel containing M. piperita essential oil as a repellent and antibacterial agent was more potent than the nanogel containing F. vulgare essential oil. Its completely protected time against Anopheles stephensi, the main malaria mosquito vector, was 120 ± 8 min. Moreover, the growth of Escherichia coli and Staphylococcus aureus after treatment with 5000 µg/mL of nanogel containing M. piperita essential oil was reduced by 100 and 65%, respectively. Considering natural constituents, a straightforward preparation method, and high efficacy, the nanogel containing M. piperita essential oil could be introduced for further investigation against other mosquitoes and bacterial species.

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Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials.

Autores: Antonio Rosato, Alessia Carocci, Alessia Catalano, Maria Lisa Clodoveo, Carlo Franchini, Filomena Corbo, Giuseppe Gerardo Carbonara, Antonio Carrieri, Giuseppe Fracchiolla | Ano: 2018 | Database: pubmed

Resumo: Mentha piperita L. essential oil (EO) is employed for external use as antipruritic, astringent, rubefacient and antiseptic. Several studies demonstrated its significant antiviral, antifungal and antibacterial properties. The aim of this work is the study of the synergistic effects of M. piperita EO with antibacterials and antifungals that are widely available and currently prescribed in therapies against infections. The observed strong synergy may constitute a potential new approach to counter the increasing phenomenon of multidrug resistant bacteria and fungi. In vitro efficacy of the association M. piperita EO/drugs was evaluated against a large panel of Gram-positive and Gram-negative bacteria and yeast strains. The antimicrobial effects were studied by checkerboard microdilution method. The synergistic effect of M. piperita EO with gentamicin resulted in a strong growth inhibition for all the bacterial species under study. The synergistic effect observed for M. piperita EO and antifungals was less pronounced.

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Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials.

Autores: Antonio Rosato, Alessia Carocci, Alessia Catalano, Maria Lisa Clodoveo, Carlo Franchini, Filomena Corbo, Giuseppe Gerardo Carbonara, Antonio Carrieri, Giuseppe Fracchiolla | Ano: 2018 | Database: pubmed

Resumo: Mentha piperita L. essential oil (EO) is employed for external use as antipruritic, astringent, rubefacient and antiseptic. Several studies demonstrated its significant antiviral, antifungal and antibacterial properties. The aim of this work is the study of the synergistic effects of M. piperita EO with antibacterials and antifungals that are widely available and currently prescribed in therapies against infections. The observed strong synergy may constitute a potential new approach to counter the increasing phenomenon of multidrug resistant bacteria and fungi. In vitro efficacy of the association M. piperita EO/drugs was evaluated against a large panel of Gram-positive and Gram-negative bacteria and yeast strains. The antimicrobial effects were studied by checkerboard microdilution method. The synergistic effect of M. piperita EO with gentamicin resulted in a strong growth inhibition for all the bacterial species under study. The synergistic effect observed for M. piperita EO and antifungals was less pronounced.

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Lousicidal, ovicidal and repellent efficacy of some essential oils against lice and flies infesting water buffaloes in Egypt.

Autores: Hanem F Khater, Mohamed Y Ramadan, Reham S El-Madawy | Ano: 2009 | Database: pubmed

Resumo: The lousicidal and repellent effects of five essential oils were investigated for the first time against the buffalo louse, Haematopinus tuberculatus, and flies infesting water buffaloes in Qalyubia Governorate, Egypt. For the in vitro studies, filter paper contact bioassays were used to test the oils and their lethal activities were compared with that of d-phenothrin. Four minutes post-treatment, the median lethal concentration, LC50, values were 2.74, 7.28, 12.35, 18.67 and 22.79% for camphor (Cinnamomum camphora), onion (Allium cepa), peppermint (Mentha piperita), chamomile (Matricaria chamomilla) and rosemary oils (Rosmarinus officinalis), respectively, whereas for d-phenothrin, it was 1.17%. The lethal time (50) (LT50) values were 0.89, 2.75, 15.39, 21.32, 11.60 and 1.94 min after treatment with 7.5% camphor, onion, peppermint, chamomile, rosemary and d-phenothrin, respectively. All the materials used except rosemary, which was not applied, were ovicidal to the eggs of H. tuberculatus. Despite the results of the in vitro assays, the in vivo treatments revealed that the pediculicidal activity was more pronounced with oils. All treated lice were killed after 0.5-2 min, whereas with d-phenothrin, 100% mortality was reached only after 120 min. The number of lice infesting buffaloes was significantly reduced 3, 6, 4, 6 and 9 days after treatment with camphor, peppermint, chamomile, onion, and d-phenothrin, respectively. Moreover, the oils and d-phenothrin significantly repelled flies, Musca domestica, Stomoxys calcitrans, Haematobia irritans and Hippobosca equina, for 6 and 3 days post-treatment, respectively. No adverse effects were noted on either animals or pour-on operators after exposure to the applied materials. Consequently, some Egyptian essential oils show potential for the development of new, speedy and safe lousicides and insect repellents for controlling lice and flies which infest water buffaloes.

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Efficacy of 11 Brazilian essential oils on lethality of the cattle tick Rhipicephalus (Boophilus) microplus.

Autores: Ana Carolina de Souza Chagas, Márcia Cristina de Sena Oliveira, Rodrigo Giglioti, Raul Costa Mascarenhas Santana, Humberto Ribeiro Bizzo, Paola Ervatti Gama, Francisco Celio Maia Chaves | Ano: 2016 | Database: pubmed

Resumo: Herbal extracts have been investigated as an alternative for parasite control, aiming to slow the development of resistance and to obtain low-cost biodegradable parasiticides. The goal of this study was to evaluate the efficacy, in vitro, of 11 essential oils from Brazil on reproductive efficiency and lethality of the cattle tick Rhipicephalus (Boophilus) microplus. The effects of oils extracted from Curcuma longa, Zingiber officinale, Lippia alba, Lippia gracilis, Lippia origanoides, Lippia sidoides, Mentha arvensis, Mentha piperita, Croton cajucara (white and red), and Croton sacaquinha on ticks were investigated by the Immersion Test with Engorged Females (ITEF) and the modified Larval Packet Test (LPT). Distilled water and 2% Tween 80 were used as control treatments. Chemical analysis of the oils was done with gas chromatography coupled with mass spectrometry. Analysis of the in vitro tests using Probit (SAS program) allowed the calculation of lethal concentrations (LCs). Lower reproductive efficiency indexes and higher efficacy percentages in the ITEF were obtained with the oils extracted from C. longa (24 and 71%, respectively) and M. arvensis oils (27 and 73%, respectively). Lower LC50 was reached with C. longa (10.24 mg/mL), L. alba (10.78 mg/mL), M. arvensis (22.31 mg/mL), L. sidoides (27.67 mg/mL), and C. sacaquinha (29.88 mg/mL) oils. In the LPT, species from Zingiberaceae and Verbenaceae families caused 100% lethality at 25 mg/mL, except for L. sidoides. The most effective oils were from C. longa, L. gracilis, L. origanoides, L. alba, and Z. officinale. The LC50 and LC90 were, respectively: 0.54 and 1.80 mg/mL, 3.21 and 7.03 mg/mL, 3.10 and 8.44 mg/mL, 5.85 and 11.14 mg/mL, and 7.75 and 13.62 mg/mL. The efficacy was directly related to the major components in each essential oil, and the oils derived from Croton genus presented the worst performance, suggesting the absence of synergistic effect among its compounds. Since C. longa, containing 62% turmerone, was the one most efficient against ticks, this compound may be potentially used for tick control, but further research is needed, especially to assess toxicity of these compounds to the host. These new studies, together with the results presented here, may provide a strong rationale for designing pre-clinical and clinical studies with these agents.

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Characterisation of peppermint (Mentha piperita L.) essential oil encapsulates.

Autores: Murat Yilmaztekin, Steva Lević, Ana Kalušević, Mustafa Cam, Branko Bugarski, Vesna Rakić, Vladimir Pavlović, Viktor Nedović | Ano: 2019 | Database: pubmed

Resumo: Aim: The aim was to choose the optimal encapsulation system and to incorporate encapsulates loaded with essential oil into the ice cream as a model food product. Methods: Ca-alginate beads were produced by electrostatic extrusion process. Gelatine/alginate coacervates were processed with coacervation. Carnauba wax microparticles were produced using melt dispersion process. Morphological properties, chemical, and thermal stabilities of encapsulates were tested by SEM, FTIR spectral, and thermogravimetric analysis. Results: Alginate provided sufficient emulsion stability over 1 h. Ca-alginate showed higher encapsulation efficiency (EE) (98.4 ± 4.3%) compared to carnauba wax (94.2 ± 7.8%) and gelatine/alginate coacervates (13.2 ± 1.2%). The presence of essential oil in all three types of encapsulates confirmed with FTIR. The encapsulation process ensured controlled release and thermal stability of the oil. Conclusions: Ca-alginate matrix as the most suitable for peppermint essential oil encapsulation. The sensory analysis showed that ice cream incorporating encapsulates is a promising system for the consumption of health beneficial peppermint essential oil.

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Characterisation of peppermint (Mentha piperita L.) essential oil encapsulates.

Autores: Murat Yilmaztekin, Steva Lević, Ana Kalušević, Mustafa Cam, Branko Bugarski, Vesna Rakić, Vladimir Pavlović, Viktor Nedović | Ano: 2019 | Database: pubmed

Resumo: Aim: The aim was to choose the optimal encapsulation system and to incorporate encapsulates loaded with essential oil into the ice cream as a model food product. Methods: Ca-alginate beads were produced by electrostatic extrusion process. Gelatine/alginate coacervates were processed with coacervation. Carnauba wax microparticles were produced using melt dispersion process. Morphological properties, chemical, and thermal stabilities of encapsulates were tested by SEM, FTIR spectral, and thermogravimetric analysis. Results: Alginate provided sufficient emulsion stability over 1 h. Ca-alginate showed higher encapsulation efficiency (EE) (98.4 ± 4.3%) compared to carnauba wax (94.2 ± 7.8%) and gelatine/alginate coacervates (13.2 ± 1.2%). The presence of essential oil in all three types of encapsulates confirmed with FTIR. The encapsulation process ensured controlled release and thermal stability of the oil. Conclusions: Ca-alginate matrix as the most suitable for peppermint essential oil encapsulation. The sensory analysis showed that ice cream incorporating encapsulates is a promising system for the consumption of health beneficial peppermint essential oil.

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Chitosan Nanoparticle Encapsulation of Antibacterial Essential Oils.

Autores: Arvind Negi, Kavindra Kumar Kesari | Ano: 2022 | Database: pubmed

Resumo: Chitosan is the most suitable encapsulation polymer because of its natural abundance, biodegradability, and surface functional groups in the form of free NH2 groups. The presence of NH2 groups allows for the facile grafting of functionalized molecules onto the chitosan surface, resulting in multifunctional materialistic applications. Quaternization of chitosan's free amino is one of the typical chemical modifications commonly achieved under acidic conditions. This quaternization improves its ionic character, making it ready for ionic-ionic surface modification. Although the cationic nature of chitosan alone exhibits antibacterial activity because of its interaction with negatively-charged bacterial membranes, the nanoscale size of chitosan further amplifies its antibiofilm activity. Additionally, the researcher used chitosan nanoparticles as polymeric materials to encapsulate antibiofilm agents (such as antibiotics and natural phytochemicals), serving as an excellent strategy to combat biofilm-based secondary infections. This paper provided a summary of available carbohydrate-based biopolymers as antibiofilm materials. Furthermore, the paper focuses on chitosan nanoparticle-based encapsulation of basil essential oil (Ocimum basilicum), mandarin essential oil (Citrus reticulata), Carum copticum essential oil ("Ajwain"), dill plant seed essential oil (Anethum graveolens), peppermint oil (Mentha piperita), green tea oil (Camellia sinensis), cardamom essential oil, clove essential oil (Eugenia caryophyllata), cumin seed essential oil (Cuminum cyminum), lemongrass essential oil (Cymbopogon commutatus), summer savory essential oil (Satureja hortensis), thyme essential oil, cinnamomum essential oil (Cinnamomum zeylanicum), and nettle essential oil (Urtica dioica). Additionally, chitosan nanoparticles are used for the encapsulation of the major essential components carvacrol and cinnamaldehyde, the encapsulation of an oil-in-water nanoemulsion of eucalyptus oil (Eucalyptus globulus), the encapsulation of a mandarin essential oil nanoemulsion, and the electrospinning nanofiber of collagen hydrolysate-chitosan with lemon balm (Melissa officinalis) and dill (Anethum graveolens) essential oil.

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Response of growth, essential oil composition, endogenous hormones and microbial activity of Mentha piperita to some organic and biofertilizers agents.

Autores: Salem M Al-Amri | Ano: 2021 | Database: pubmed

Resumo: The effect of organic (poultry and cattle manures) and biological (effective microorganisms, EM) fertilizers on growth, essential oil yield and its compositions, endogenous phytohormones content and antibacterial activity of peppermint plants grown in pot over 12 weeks was studied. Application of organo- and bio-fertilizers greatly affected on growth, essential oil production and other estimated parameters of peppermint plants. Slight stimulation effect was happened due to soil application of organic manures. Soil application of EM alone or in combination with organic fertilizers significantly increased growth, yield and components of essential oils, endogenous hormones of peppermint as compared to other treatments. Using disc diffusion method, the extracted oil of peppermint plants amended with organic and biofertilizers recorded the highest antibacterial activity against tested pathogenic bacteria like Klebsiella pneuumoniae and Staphylococcus aureus.

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Response of growth, essential oil composition, endogenous hormones and microbial activity of Mentha piperita to some organic and biofertilizers agents.

Autores: Salem M Al-Amri | Ano: 2021 | Database: pubmed

Resumo: The effect of organic (poultry and cattle manures) and biological (effective microorganisms, EM) fertilizers on growth, essential oil yield and its compositions, endogenous phytohormones content and antibacterial activity of peppermint plants grown in pot over 12 weeks was studied. Application of organo- and bio-fertilizers greatly affected on growth, essential oil production and other estimated parameters of peppermint plants. Slight stimulation effect was happened due to soil application of organic manures. Soil application of EM alone or in combination with organic fertilizers significantly increased growth, yield and components of essential oils, endogenous hormones of peppermint as compared to other treatments. Using disc diffusion method, the extracted oil of peppermint plants amended with organic and biofertilizers recorded the highest antibacterial activity against tested pathogenic bacteria like Klebsiella pneuumoniae and Staphylococcus aureus.

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Oligochitosan fortifies antioxidative and photosynthetic metabolism and enhances secondary metabolite accumulation in arsenic-stressed peppermint.

Autores: Bilal Ahmad, Tariq Ahmad Dar, M Masroor A Khan, Ajaz Ahmad, Jörg Rinklebe, Yinglong Chen, Parvaiz Ahmad | Ano: 2022 | Database: pubmed

Resumo: The current study was designed to investigate whether application of irradiated chitosan (ICn), a recently established plant growth promoter, can prove effective in alleviating arsenic (As) stress in peppermint, a medicinally important plant. This study investigated how foliar application of ICn alleviated As toxicity in peppermint (Mentha piperita L.). Peppermint plants were treated with ICn (80 mg L-1) alone or in combination with As (10, 20, or 40 mg kg-1 of soil, as Na2HAsO4·7H2O) 40 days after transplantation (DAT), and effects on the growth, photosynthesis, and antioxidants were assessed at 150 DAT as stress severely decreases plant growth, affects photosynthesis, and alters enzymatic (ascorbate peroxidase, superoxide dismutase) and non-enzymatic (glutathione) antioxidants. When applied at 40 mg kg-1, ICn significantly decreased the content of essential oil (EO) and total phenols in peppermint by 13.8 and 16.0%, respectively, and decreased phenylalanine ammonia lyase (PAL) and deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) activities by 12.8 and 14.6%, respectively. Application of ICn mitigated the disadvantageous effects caused by As toxicity in peppermint by enhancing activities of antioxidative enzymes and photosynthesis and increased accretion of secondary metabolism products (EOs and phenols). An enhancement of total phenols (increased by 17.3%) and EOs (36.4%) is endorsed to ICn-stimulated enhancement in the activities of PAL and DXR (65.9 and 28.9%, respectively) in comparison to the control. To conclude, this study demonstrated that foliar application of ICn (80 mgL-1) effectively promoted the growth and physiology of peppermint and eliminated As-induced toxicity to achieve high production of EO-containing crops grown in metal-contaminated soils.

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Hydrodistillation and in situ microwave-generated hydrodistillation of fresh and dried mint leaves: a comparison study.

Autores: Laura Orio, Giancarlo Cravotto, Arianna Binello, Giuseppe Pignata, Silvana Nicola, Farid Chemat | Ano: 2012 | Database: pubmed

Resumo: Hydrodistillation (HD) has been used since ancient times for the extraction of essential oils (EO). Despite the intrinsic limitations of this technique, it remains the most common method both in the laboratory and on an industrial scale. The main drawbacks are the long extraction time involved and the risk of thermal degradation. Over the last decade, microwave-assisted extraction (MAE) and in situ microwave-generated hydrodistillation (MGH) have been shown to be the most promising techniques in improving plant extraction and hydrodistillation.

Resultados: In this study we compare HD with MGH in the extraction of several mint species cultivated in Piedmont: Mentha spicata L. var. rubra, Mentha spicata L. var. viridis and Mentha piperita L. MGH requires either fresh plant or rehydrated material, it is extremely fast and allows a reduction in energy consumption and overall cost. All the EO have been analyzed by gas chromatography-mass spectrometry. A mechanism of microwave-generated essential oil extraction has been proposed to explain the differences in the composition of the oil obtained from this environmentally friendly technique.

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Extraction of Peppermint Essential Oils and Lipophilic Compounds: Assessment of Process Kinetics and Environmental Impacts with Multiple Techniques.

Autores: Aleksandar Radivojac, Oskar Bera, Zoran Zeković, Nemanja Teslić, Živan Mrkonjić, Danijela Bursać Kovačević, Predrag Putnik, Branimir Pavlić | Ano: 2021 | Database: pubmed

Resumo: Consumers are becoming more mindful of their well-being. Increasing awareness of the many beneficial properties of peppermint essential oil (EO) has significantly increased product sales in recent years. Hydrodistillation (HD), a proven conventional method, and a possible alternative in the form of microwave-assisted hydrodistillation (MWHD) have been used to isolate peppermint EO. Standard Soxhlet and alternatively supercritical fluid (SFE), microwave-assisted, and ultrasound-assisted extraction separated the lipid extracts. The distillations employed various power settings, and the EO yield varied from 0.15 to 0.80%. The estimated environmental impact in terms of electricity consumption and CO2 emissions suggested that MWHD is an energy efficient way to reduce CO2 emissions. Different extraction methods and solvent properties affected the lipid extract yield, which ranged from 2.55 to 5.36%. According to the corresponding values of statistical parameters, empiric mathematical models were successfully applied to model the kinetics of MWHD and SFE processes.

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Oligochitosan fortifies antioxidative and photosynthetic metabolism and enhances secondary metabolite accumulation in arsenic-stressed peppermint.

Autores: Bilal Ahmad, Tariq Ahmad Dar, M Masroor A Khan, Ajaz Ahmad, Jörg Rinklebe, Yinglong Chen, Parvaiz Ahmad | Ano: 2022 | Database: pubmed

Resumo: The current study was designed to investigate whether application of irradiated chitosan (ICn), a recently established plant growth promoter, can prove effective in alleviating arsenic (As) stress in peppermint, a medicinally important plant. This study investigated how foliar application of ICn alleviated As toxicity in peppermint (Mentha piperita L.). Peppermint plants were treated with ICn (80 mg L-1) alone or in combination with As (10, 20, or 40 mg kg-1 of soil, as Na2HAsO4·7H2O) 40 days after transplantation (DAT), and effects on the growth, photosynthesis, and antioxidants were assessed at 150 DAT as stress severely decreases plant growth, affects photosynthesis, and alters enzymatic (ascorbate peroxidase, superoxide dismutase) and non-enzymatic (glutathione) antioxidants. When applied at 40 mg kg-1, ICn significantly decreased the content of essential oil (EO) and total phenols in peppermint by 13.8 and 16.0%, respectively, and decreased phenylalanine ammonia lyase (PAL) and deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) activities by 12.8 and 14.6%, respectively. Application of ICn mitigated the disadvantageous effects caused by As toxicity in peppermint by enhancing activities of antioxidative enzymes and photosynthesis and increased accretion of secondary metabolism products (EOs and phenols). An enhancement of total phenols (increased by 17.3%) and EOs (36.4%) is endorsed to ICn-stimulated enhancement in the activities of PAL and DXR (65.9 and 28.9%, respectively) in comparison to the control. To conclude, this study demonstrated that foliar application of ICn (80 mgL-1) effectively promoted the growth and physiology of peppermint and eliminated As-induced toxicity to achieve high production of EO-containing crops grown in metal-contaminated soils.

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Mentha piperita L. essential oil inactivates spoilage yeasts in fruit juices through the perturbation of different physiological functions in yeast cells.

Autores: Erika Tayse da Cruz Almeida, Geany Targino de Souza, Jossana Pereira de Sousa Guedes, Isabella Medeiros Barbosa, Cristina Paiva de Sousa, Lúcio Roberto Cançado Castellano, Marciane Magnani, Evandro Leite de Souza | Ano: 2019 | Database: pubmed

Resumo: This study evaluated the efficacy of the essential oil from Mentha piperita L. (MPEO) to inactivate cells of the potentially spoilage yeasts Candida albicans, Candida tropicalis, Pichia anomala and Saccharomyces cerevisiae in cashew, guava, mango and pineapple juices during 72 h of refrigerated storage. Damage in different physiological functions caused by MPEO in S. cerevisiae in cashew and guava juices were investigated using flow cytometry (FC). The effects of the incorporation of an effective anti-yeast MPEO dose on sensory characteristics of juices were also evaluated. MPEO displayed minimum inhibitory concentration of 1.875 μL/mL against all tested yeasts. A >5 log reduction in counts of C. albicans, P. anomala and S. cerevisiae was observed in cashew and guava juices with 7.5 and 3.75 μL/mL MPEO. Tested MPEO concentrations (1.875, 3.75 and 7.5 μL/mL) were not effective to cause >5 log reduction in counts of target yeasts in mango and pineapple juices during 72 h of exposure. Incorporation of 1.875 μL/mL MPEO in cashew and guava juices strongly compromised membrane permeability, membrane potential, enzymatic activity and efflux pump activity in S. cerevisiae cells. This same MPEO concentration did not affect appearance, odor and viscosity in fruit juices, but negatively affected their taste and aftertaste. These results show the efficacy of MPEO to inactivate potentially spoilage yeasts in fruit juices through disturbance of different physiological functions in yeast cells. However, the combined use of MPEO with other technologies should be necessary to decrease its effective anti-yeast dose in fruit juices and, consequently, the possible negative impacts on specific sensory properties of these products.

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Gamma irradiation-induced variability in morpho-agronomic and oil quality traits of Mentha piperita L.

Autores: Priyanka Prasad, Vagmi Singh, Nashra Aftab, Akancha Gupta, Ram Kishor, Himanshu Kumar Kushwaha, Vivek Singh, Ram Swaroop Verma, Birendra Kumar | Ano: 2021 | Database: pubmed

Resumo: To develop elite mutant in Mentha piperita having morphotype and oil content of Mentha arvensis, and oil quality of M. piperita.

Resultados: The developed and selected superior mutants viz. MPK-5(1) and MPK-5(3) have a mean herb yield potential of 23.923 and 21.503 Kg/9 m2; mean oil yield of 92.953 and 80.047 ml/plot; mean menthol content of 69.012% and 69.160% with mean menthofuran content of 1.554% and 0.531%, respectively.

Conclusão: N/A

Mentha piperita essential oil induces apoptosis in yeast associated with both cytosolic and mitochondrial ROS-mediated damage.

Autores: Patrícia Ferreira, Teresa Cardoso, Filipa Ferreira, Manuel Fernandes-Ferreira, Peter Piper, Maria João Sousa | Ano: 2014 | Database: pubmed

Resumo: Mentha piperita (MP), also known as peppermint, is an aromatic and medicinal plant widely used in the food industry, perfumery and cosmetic, pharmacy and traditional medicine. Its essential oil (EO) displays antimicrobial activity against a range of bacteria and fungi. In this study, we found that MP EO lethal cytotoxicity is associated with increased levels of intracellular reactive oxygen species, mitochondrial fragmentation and chromatin condensation, without loss of the plasma membrane integrity, indicative of an apoptotic process. Overexpression of cytosolic catalase and superoxide dismutases reverted the lethal effects of the EO and of its major component menthol. Conversely, deficiency in Sod1p (cytosolic copper-zinc-superoxide dismutase) greatly increased sensitivity to both agents, but deficiency in Sod2p (mitochondrial manganese superoxide dismutase) only induced sensitivity under respiratory growth conditions. Mentha piperita EO increased the frequency of respiratory deficient mutants indicative of damage to the mitochondrial genome, although increase in mitochondrial thiol oxidation does not seem to be involved in the EO toxicity.

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Conclusão: N/A

Mentha piperita essential oil induces apoptosis in yeast associated with both cytosolic and mitochondrial ROS-mediated damage.

Autores: Patrícia Ferreira, Teresa Cardoso, Filipa Ferreira, Manuel Fernandes-Ferreira, Peter Piper, Maria João Sousa | Ano: 2014 | Database: pubmed

Resumo: Mentha piperita (MP), also known as peppermint, is an aromatic and medicinal plant widely used in the food industry, perfumery and cosmetic, pharmacy and traditional medicine. Its essential oil (EO) displays antimicrobial activity against a range of bacteria and fungi. In this study, we found that MP EO lethal cytotoxicity is associated with increased levels of intracellular reactive oxygen species, mitochondrial fragmentation and chromatin condensation, without loss of the plasma membrane integrity, indicative of an apoptotic process. Overexpression of cytosolic catalase and superoxide dismutases reverted the lethal effects of the EO and of its major component menthol. Conversely, deficiency in Sod1p (cytosolic copper-zinc-superoxide dismutase) greatly increased sensitivity to both agents, but deficiency in Sod2p (mitochondrial manganese superoxide dismutase) only induced sensitivity under respiratory growth conditions. Mentha piperita EO increased the frequency of respiratory deficient mutants indicative of damage to the mitochondrial genome, although increase in mitochondrial thiol oxidation does not seem to be involved in the EO toxicity.

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Conclusão: N/A

Inhibition by the essential oils of peppermint and spearmint of the growth of pathogenic bacteria.

Autores: H Imai, K Osawa, H Yasuda, H Hamashima, T Arai, M Sasatsu | Ano: 2001 | Database: pubmed

Resumo: The effects of the, essential oils of peppermint (Mentha piperita L.), spearmint Mentha spicata L.) and Japanese mint (Mentha, arvensis L.), of four major constituents of the esssential oil of peppermint, and of three major constituents of the essential oil of spearmint, on the proliferation of Helicobacter pylori, Salmonella enteritidis, Escherichia coli O157:H7, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococccus aureus (MSSA) were examined. The essential oils and the various constituents inhibited the proliferation of each strain in liquid culture in a dose-dependent manner. In addition, they exhibited bactericidal activity in phosphate-buffered saline. The antibacterial activities varied among the bacterial species tested but were almost the same against antibiotic-resistant and antibiotic-sensitive strains of Helicobacter pylori and S. aureus. Thus, the essential oils and their constituents may be useful as potential antibacterial agents for inhibition of the growth of pathogens.

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Conclusão: N/A

Synergistic mixtures of chitosan and Mentha piperita L. essential oil to inhibit Colletotrichum species and anthracnose development in mango cultivar Tommy Atkins.

Autores: Kataryne Árabe Rimá de Oliveira, Lúcia Raquel Ramos Berger, Samara Amorim de Araújo, Marcos Paz Saraiva Câmara, Evandro Leite de Souza | Ano: 2017 | Database: pubmed

Resumo: This study assessed the efficacy of chitosan (CHI) and Mentha piperita L. essential oil (MPEO) alone or in combination to control the mycelial growth of five different Colletotrichum species, C. asianum, C. dianesei, C. fructicola, C. tropicale and C. karstii, identified as potential anthracnose-causing agents in mango (Mangifera indica L.). The efficacy of coatings of CHI and MPEO mixtures in controlling the development of anthracnose in mango cultivar Tommy Atkins was evaluated. CHI (2.5, 5, 7.5 and 10 mg/mL) and MPEO (0.3, 0.6, 1.25, 2.5 and 5 μL/mL) alone effectively inhibited mycelial growth of all tested Colletotrichum strains in synthetic media. Mixtures of CHI (5 or 7.5 mg/mL) and MPEO (0.3, 0.6 or 1.25 μL/mL) strongly inhibited mycelial growth and showed additive or synergistic inhibitory effects on the tested Colletotrichum strains based on the Abbott index. The application of coatings of CHI (5 or 7.5 mg/mL) and MPEO (0.6 or 1.25 μL/mL) mixtures that presented synergistic interactions decreased anthracnose lesion severity in mango artificially contaminated with either of the tested Colletotrichum strains over 15 days of storage at 25 °C. The anthracnose lesion severity in mango coated with the mixtures of CHI and MPEO was similar or lower than those observed in mango treated with the synthetic fungicides thiophanate-methyl (10 μg a.i./mL) and difenoconazole (0.5 μg a.i./mL). The application of coatings containing low doses of CHI and MPEO may be an effective alternative for controlling the postharvest development of anthracnose in mango cultivar Tommy Atkins.

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Conclusão: N/A

Toxicity and repellent activity of essential oil from Mentha piperita Linn. leaves and its major monoterpenoids against three stored product insects.

Autores: Xue Pang, Yi-Xi Feng, Xiao-Jie Qi, Yang Wang, Borjigidai Almaz, Chao Xi, Shu-Shan Du | Ano: 2020 | Database: pubmed

Resumo: The essential oil (EO) from leaves of Mentha piperita was extracted by hydrodistillation. Twenty-one chemical components, accounting for 97.5% of the total oil, were determined by GC-MS and GC-FID. The major chemical components included menthol (41.6%), L-menthone (24.7%), isomenthol (6.3%), and limonene (5.0%). The bioactivity of the obtained EO and its two major components against Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults were evaluated by fumigation, contact, and repellent activity bioassay. The EO showed significant fumigation and contact toxicity against T. castaneum (LC50 = 18.1 mg/L air and LD50 = 2.9 μg/adult, respectively), L. serricorne (LC50 = 68.4 mg/L air and LD50 = 12.6 μg/adult, respectively), and L. bostrychophila (LC50 = 0.6 mg/L air and LD50 = 49.8 μg/adult, respectively) adults. Meanwhile, the repellent effect of the EO on T. castaneum and L. serricorne adults was comparable to that of the positive control at the highest tested concentration. Menthol and L-menthone were two major components in total oil. Among them, L-menthone exhibited significant insecticidal activity on target insects, and menthol showed notable repellent effects. The results indicated that the EO of M. piperita leaves and two tested components have potential to be developed as natural insecticides and repellents for the control of stored product insect pests. Graphical abstract.

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Conclusão: N/A

Toxicity, antifeedant and biochemical efficacy of Mentha piperita L. essential oil and their major constituents against stored grain pest.

Autores: Vallavan Rajkumar, Chinappan Gunasekaran, Inbaraj Kanitha Christy, Jayaraman Dharmaraj, Panneerselvam Chinnaraj, Cheruvathur Amita Paul | Ano: 2019 | Database: pubmed

Resumo: The efficiency of chemically characterized Mentha piperita L. essential oil (EO), a plant based insecticide was tested for acetylcholinesterase activity (AChE) and antioxidant enzyme system (SOD, CAT, GSH and GSSH) in Sitophilus oryzae (L.) and Tribolium castaneum (Herbst.). GC-MS results evidenced for menthone and menthol as the major compounds of EO. As a fumigant, EO and its major component showed 100% mortality at 75.0 μL/L air and 100.0 μL/L air against S. oryzae and T. castaneum at 24 h of exposure. Antifeedant activity exhibited 100% lethal activity against pests in comparison to sub lethal doses. In vivo percent inhibition of AChE activity ranged between S. oryzae (29.68%, 18.81% and 14.34%) and T. castaneum (20.67%, 13.73% and 9.19%). Significant changes in antioxidant defense system studied for SOD, CAT, GSH and GSSH were noticed. Results revealed the toxicity of EO associations with the inhibition of AChE activity accompanied by oxidative imbalance. Therefore, M. piperita EO has a noteworthy role in insecticidal properties and could be recommended as an eco-friendly alternative to synthetic insecticides.

Resultados: N/A

Conclusão: N/A

Toxicity and Physiological Effects of Nine Lamiaceae Essential Oils and Their Major Compounds on Reticulitermes dabieshanensis.

Autores: Xi Yang, Chunzhe Jin, Ziwei Wu, Hui Han, Zhilin Zhang, Yongjian Xie, Dayu Zhang | Ano: 2023 | Database: pubmed

Resumo: The volatile metabolites of Salvia sclarea, Rosmarinus officinalis, Thymus serpyllum, Mentha spicata, Melissa officinalis, Origanum majorana, Mentha piperita, Ocimum basilicum and Lavandula angustifolia were determined by gas chromatography-mass spectrometry. The vapor insecticidal properties of the analyzed essential oils and their compounds were screened using Reticulitermes dabieshanensis workers. The most effective oils were S. sclarea (major constituent linalyl acetate, 65.93%), R. officinalis (1,8-cineole, 45.56%), T. serpyllum (thymol, 33.59%), M. spicata (carvone, 58.68%), M. officinalis (citronellal, 36.99%), O. majorana (1,8-cineole, 62.29%), M. piperita (menthol, 46.04%), O. basilicum (eugenol, 71.08%) and L. angustifolia (linalool, 39.58%), which exhibited LC50 values ranging from 0.036 to 1.670 μL/L. The lowest LC50 values were recorded for eugenol (0.060 μL/L), followed by thymol (0.062 μL/L), carvone (0.074 μL/L), menthol (0.242 μL/L), linalool (0.250 μL/L), citronellal (0.330 μL/L), linalyl acetate (0.712 μL/L) and 1,8-cineole (1.478 μL/L). The increased activity of esterases (ESTs) and glutathione S-transferase (GST) were observed but only alongside the decreased activity of acetylcholinesterase (AChE) in eight main components. Our results indicate that S. sclarea, R. officinalis, T. serpyllum, M. spicata, M. officinalis, O. marjorana, M. piperita, O. basilicum and L. angustifolia essential oils (EOs) and their compounds, linalyl acetate, 1,8-cineole, thymol, carvone, citronellal, menthol, eugenol and linalool could be developed as control agents against termites.

Resultados: N/A

Conclusão: N/A

Toxicity and Physiological Effects of Nine Lamiaceae Essential Oils and Their Major Compounds on Reticulitermes dabieshanensis.

Autores: Xi Yang, Chunzhe Jin, Ziwei Wu, Hui Han, Zhilin Zhang, Yongjian Xie, Dayu Zhang | Ano: 2023 | Database: pubmed

Resumo: The volatile metabolites of Salvia sclarea, Rosmarinus officinalis, Thymus serpyllum, Mentha spicata, Melissa officinalis, Origanum majorana, Mentha piperita, Ocimum basilicum and Lavandula angustifolia were determined by gas chromatography-mass spectrometry. The vapor insecticidal properties of the analyzed essential oils and their compounds were screened using Reticulitermes dabieshanensis workers. The most effective oils were S. sclarea (major constituent linalyl acetate, 65.93%), R. officinalis (1,8-cineole, 45.56%), T. serpyllum (thymol, 33.59%), M. spicata (carvone, 58.68%), M. officinalis (citronellal, 36.99%), O. majorana (1,8-cineole, 62.29%), M. piperita (menthol, 46.04%), O. basilicum (eugenol, 71.08%) and L. angustifolia (linalool, 39.58%), which exhibited LC50 values ranging from 0.036 to 1.670 μL/L. The lowest LC50 values were recorded for eugenol (0.060 μL/L), followed by thymol (0.062 μL/L), carvone (0.074 μL/L), menthol (0.242 μL/L), linalool (0.250 μL/L), citronellal (0.330 μL/L), linalyl acetate (0.712 μL/L) and 1,8-cineole (1.478 μL/L). The increased activity of esterases (ESTs) and glutathione S-transferase (GST) were observed but only alongside the decreased activity of acetylcholinesterase (AChE) in eight main components. Our results indicate that S. sclarea, R. officinalis, T. serpyllum, M. spicata, M. officinalis, O. marjorana, M. piperita, O. basilicum and L. angustifolia essential oils (EOs) and their compounds, linalyl acetate, 1,8-cineole, thymol, carvone, citronellal, menthol, eugenol and linalool could be developed as control agents against termites.

Resultados: N/A

Conclusão: N/A

Toxicity, antifeedant and biochemical efficacy of Mentha piperita L. essential oil and their major constituents against stored grain pest.

Autores: Vallavan Rajkumar, Chinappan Gunasekaran, Inbaraj Kanitha Christy, Jayaraman Dharmaraj, Panneerselvam Chinnaraj, Cheruvathur Amita Paul | Ano: 2019 | Database: pubmed

Resumo: The efficiency of chemically characterized Mentha piperita L. essential oil (EO), a plant based insecticide was tested for acetylcholinesterase activity (AChE) and antioxidant enzyme system (SOD, CAT, GSH and GSSH) in Sitophilus oryzae (L.) and Tribolium castaneum (Herbst.). GC-MS results evidenced for menthone and menthol as the major compounds of EO. As a fumigant, EO and its major component showed 100% mortality at 75.0 μL/L air and 100.0 μL/L air against S. oryzae and T. castaneum at 24 h of exposure. Antifeedant activity exhibited 100% lethal activity against pests in comparison to sub lethal doses. In vivo percent inhibition of AChE activity ranged between S. oryzae (29.68%, 18.81% and 14.34%) and T. castaneum (20.67%, 13.73% and 9.19%). Significant changes in antioxidant defense system studied for SOD, CAT, GSH and GSSH were noticed. Results revealed the toxicity of EO associations with the inhibition of AChE activity accompanied by oxidative imbalance. Therefore, M. piperita EO has a noteworthy role in insecticidal properties and could be recommended as an eco-friendly alternative to synthetic insecticides.

Resultados: N/A

Conclusão: N/A

Toxicity and repellent activity of essential oil from Mentha piperita Linn. leaves and its major monoterpenoids against three stored product insects.

Autores: Xue Pang, Yi-Xi Feng, Xiao-Jie Qi, Yang Wang, Borjigidai Almaz, Chao Xi, Shu-Shan Du | Ano: 2020 | Database: pubmed

Resumo: The essential oil (EO) from leaves of Mentha piperita was extracted by hydrodistillation. Twenty-one chemical components, accounting for 97.5% of the total oil, were determined by GC-MS and GC-FID. The major chemical components included menthol (41.6%), L-menthone (24.7%), isomenthol (6.3%), and limonene (5.0%). The bioactivity of the obtained EO and its two major components against Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults were evaluated by fumigation, contact, and repellent activity bioassay. The EO showed significant fumigation and contact toxicity against T. castaneum (LC50 = 18.1 mg/L air and LD50 = 2.9 μg/adult, respectively), L. serricorne (LC50 = 68.4 mg/L air and LD50 = 12.6 μg/adult, respectively), and L. bostrychophila (LC50 = 0.6 mg/L air and LD50 = 49.8 μg/adult, respectively) adults. Meanwhile, the repellent effect of the EO on T. castaneum and L. serricorne adults was comparable to that of the positive control at the highest tested concentration. Menthol and L-menthone were two major components in total oil. Among them, L-menthone exhibited significant insecticidal activity on target insects, and menthol showed notable repellent effects. The results indicated that the EO of M. piperita leaves and two tested components have potential to be developed as natural insecticides and repellents for the control of stored product insect pests. Graphical abstract.

Resultados: N/A

Conclusão: N/A

Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi.

Autores: Nitin Chauhan, Anushree Malik, Satyawati Sharma, R C Dhiman | Ano: 2016 | Database: pubmed

Resumo: The larvicidal activity of Mentha piperita, Cymbopogan citratus (lemongrass), Eucalyptus globulus and Citrus sinensis (orange) essential oils and their combinations was evaluated against Musca domestica (housefly) and Anopheles stephensi (mosquitoes) through contact toxicity assay. Among all the tested essential oils/combinations, Me. piperita was found to be the most effective larvicidal agent against Mu. domestica and An. stephensi with LC50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, after 48 h. The results clearly highlighted that the addition of mentha oil to other oils (1:1 ratio) improved their larvicidal activity. The order of effectiveness of essential oils/combinations indicated that the pattern for An. stephensi follows the trend as mentha > mentha + lemongrass > lemongrass > mentha + eucalyptus > eucalyptus > mentha + orange > orange and for Mu. domestica as mentha > mentha + lemongrass > lemongrass > mentha + orange > orange > mentha + eucalyptus > eucalyptus. The images obtained from scanning electron microscopy (SEM) analysis indicated the toxic effect of Me. piperita as the treated larvae were observed to be dehydrated and deformed. This study demonstrates the effectiveness of tested essential oils/combinations against the larval stages of Mu. domestica and An. stephensi and has the potential for development of botanical formulations.

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Conclusão: N/A

Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi.

Autores: Nitin Chauhan, Anushree Malik, Satyawati Sharma, R C Dhiman | Ano: 2016 | Database: pubmed

Resumo: The larvicidal activity of Mentha piperita, Cymbopogan citratus (lemongrass), Eucalyptus globulus and Citrus sinensis (orange) essential oils and their combinations was evaluated against Musca domestica (housefly) and Anopheles stephensi (mosquitoes) through contact toxicity assay. Among all the tested essential oils/combinations, Me. piperita was found to be the most effective larvicidal agent against Mu. domestica and An. stephensi with LC50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, after 48 h. The results clearly highlighted that the addition of mentha oil to other oils (1:1 ratio) improved their larvicidal activity. The order of effectiveness of essential oils/combinations indicated that the pattern for An. stephensi follows the trend as mentha > mentha + lemongrass > lemongrass > mentha + eucalyptus > eucalyptus > mentha + orange > orange and for Mu. domestica as mentha > mentha + lemongrass > lemongrass > mentha + orange > orange > mentha + eucalyptus > eucalyptus. The images obtained from scanning electron microscopy (SEM) analysis indicated the toxic effect of Me. piperita as the treated larvae were observed to be dehydrated and deformed. This study demonstrates the effectiveness of tested essential oils/combinations against the larval stages of Mu. domestica and An. stephensi and has the potential for development of botanical formulations.

Resultados: N/A

Conclusão: N/A

Larvicidal and adulticidal activity of essential oils from plants of the Lamiaceae family against the West Nile virus vector, Culex pipiens (Diptera: Culicidae).

Autores: Hanan Abo El-Kasem Bosly | Ano: 2022 | Database: pubmed

Resumo: Culex pipiens mosquitoes are the most widely distributed primary vector of the West Nile virus worldwide. Many attempts for investigation of botanical pesticides to avoid the development of pesticide resistance to conventional synthetic pesticides that are recognized as a threat to the diversity of ecosystems. The study aimed to determine the components of three essential oils of Lamiaceae family, lavender (Lavandula angustifolia), peppermint (Mentha piperita L.), and rosemary (Rosmarinus officinalis L.) by gas chromatography-mass spectrometry (GC-MS) analysis. Furthermore, aimed to validate the insecticidal activities of these oils as larvicidal agents against the third instar larvae of Culex pipiens using five different concentrations (62.5, 125, 250, 500, and 1000 ppm) for each oil in five replicates and as an adulticidal agent against approximately three-day-old female adults of Cx. Pipiens using 0.5, 1, 2, 4, and 5% concentrations in three replicates. The results generally showed a dose-related response. At 1000 ppm, rosemary oil showed the highest larvicidal (100%) (LC50, 214.97 ppm), followed by peppermint oil (92.00% mortality and LC50 (269.35 ppm). Lavender oil showed the lowest efficacy with 87.20% mortality and LC50 (301.11 ppm). At 5% oil concentration, the highest knockdown rate at 1 h was recorded for lavender oil (95.55%), followed by peppermint oil (88.89%) and lastly rosemary oil (84.44%). After 24 h, rosemary oil showed the lowest adult mortality rate (88.89%; LC50, 1.44%), while lavender and peppermint oils both showed a 100% mortality rate, with (LC50, 0.81% and 0.91%, respectively). The chemical constituents of the oils consisted of monoterpenes and sesquiterpenes that determined their insecticidal activities against the target insect stage. The study proposed that rosemary essential oil may be useful for the control of Cx. pipiens larvae as part of an integrated water treatment strategy, and lavender and peppermint oils may be used in an integrated plan for adult's control.

Resultados: N/A

Conclusão: N/A

Larvicidal and adulticidal activity of essential oils from plants of the Lamiaceae family against the West Nile virus vector, Culex pipiens (Diptera: Culicidae).

Autores: Hanan Abo El-Kasem Bosly | Ano: 2022 | Database: pubmed

Resumo: Culex pipiens mosquitoes are the most widely distributed primary vector of the West Nile virus worldwide. Many attempts for investigation of botanical pesticides to avoid the development of pesticide resistance to conventional synthetic pesticides that are recognized as a threat to the diversity of ecosystems. The study aimed to determine the components of three essential oils of Lamiaceae family, lavender (Lavandula angustifolia), peppermint (Mentha piperita L.), and rosemary (Rosmarinus officinalis L.) by gas chromatography-mass spectrometry (GC-MS) analysis. Furthermore, aimed to validate the insecticidal activities of these oils as larvicidal agents against the third instar larvae of Culex pipiens using five different concentrations (62.5, 125, 250, 500, and 1000 ppm) for each oil in five replicates and as an adulticidal agent against approximately three-day-old female adults of Cx. Pipiens using 0.5, 1, 2, 4, and 5% concentrations in three replicates. The results generally showed a dose-related response. At 1000 ppm, rosemary oil showed the highest larvicidal (100%) (LC50, 214.97 ppm), followed by peppermint oil (92.00% mortality and LC50 (269.35 ppm). Lavender oil showed the lowest efficacy with 87.20% mortality and LC50 (301.11 ppm). At 5% oil concentration, the highest knockdown rate at 1 h was recorded for lavender oil (95.55%), followed by peppermint oil (88.89%) and lastly rosemary oil (84.44%). After 24 h, rosemary oil showed the lowest adult mortality rate (88.89%; LC50, 1.44%), while lavender and peppermint oils both showed a 100% mortality rate, with (LC50, 0.81% and 0.91%, respectively). The chemical constituents of the oils consisted of monoterpenes and sesquiterpenes that determined their insecticidal activities against the target insect stage. The study proposed that rosemary essential oil may be useful for the control of Cx. pipiens larvae as part of an integrated water treatment strategy, and lavender and peppermint oils may be used in an integrated plan for adult's control.

Resultados: N/A

Conclusão: N/A

Bioefficacy of Mentha piperita essential oil against dengue fever mosquito Aedes aegypti L.

Autores: Sarita Kumar, Naim Wahab, Radhika Warikoo | Ano: 2011 | Database: pubmed

Resumo: To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant, Mentha piperita (M. piperita) against the larval and adult stages of Aedes aegypti (Ae. Aegypti).

Resultados: The essential oil extracted from M. piperita possessed excellent larvicidal efficiency against dengue vector. The bioassays showed an LC50 and LC90 value of 111.9 and 295.18 ppm, respectively after 24 h of exposure. The toxicity of the oil increased 11.8% when the larvae were exposed to the oil for 48 h. The remarkable repellent properties of M. piperita essential oil were established against adults Ae. aegypti. The application of oil resulted in 100% protection till 150 min. After next 30 min, only 1-2 bites were recorded as compared with 8-9 bites on the control arm.

Conclusão: N/A

Retention of aroma compounds from Mentha piperita essential oil by cyclodextrins and crosslinked cyclodextrin polymers.

Autores: A Ciobanu, I Mallard, D Landy, G Brabie, D Nistor, S Fourmentin | Ano: 2013 | Database: pubmed

Resumo: In this paper, the controlled release of aroma compounds from cyclodextrins (CDs) and CD polymers was studied by multiple headspace extraction (MHE) experiments. Mentha piperita essential oil was obtained by Soxhlet extraction and identification of the major compounds was performed by GC-MS analysis. Menthol, menthone, pulegone and eucalyptol were identified as the major components. Retention of standard compounds in the presence of different CDs and CD polymers has been realised by static headspace gas chromatography (SH-GC) at 25 °C in the aqueous or gaseous phase. Stability constants for standard compounds and for compounds in essential oil have been also determined with monomeric CD derivatives. The obtained results indicated the formation of a 1:1 inclusion complex for all the studied compounds. Molecular modelling was used to investigate the complementarities between host and guest. This study showed that β-CDs were the most versatile CDs and that β-CD polymers could perform the controlled release of aroma compounds.

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Conclusão: N/A

Chromosome doubling influences the morphological, physiological, biochemical and genetic traits related to essential oil biosynthesis of peppermint (Mentha piperita) under salinity stress.

Autores: Zhongjuan Zhao, Yanli Wei, Ling Li, Baojun Liu, Kai Yang, Hetong Yang, Jishun Li | Ano: 2022 | Database: pubmed

Resumo: Peppermint (Mentha piperita L.) is an important medicinal aromatic plant. In this study, the morphology, physiology, biochemistry and gene expression of chromosomes doubling peppermint (D1 lines) were analyzed. The analysis showed that D1 lines had larger, thicker and darker leaves, and stronger roots when planted in the pots, but delayed growth in the field condition. Under NaCl stress, the D1 lines increased cell oxidative defense through more active antioxidant enzymes and decreased the oxidative damages of cell membrane, leading to a significantly greater survival rate and photosynthesis intensity than WT lines. The size and density of glandular trichomes of D1 lines was larger, which contributed to its higher essential oil yield. In addition, chromosome doubling reduced the inhibition of NaCl stress on essential oil yield and quality, through changing the expression of genes in the oil biosynthesis pathway. The traits of chromosome doubling peppermint provide new technical and theoretical evidence for peppermint germplasm improvement.

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Comparative Study on Microencapsulation of Lavender (Lavandula angustifolia Mill.) and Peppermint (Mentha piperita L.) Essential Oils via Spray-Drying Technique.

Autores: Bissera Pilicheva, Yordanka Uzunova, Plamen Katsarov | Ano: 2021 | Database: pubmed

Resumo: Essential oils have been studied for various applications, including for therapeutic purposes. There is extensive literature regarding their properties; however, their low stability limits their application. Generally, the microencapsulation of essential oils allows enhanced stability and enables the potential incorporation in solid dosage forms. Lavender and peppermint oils were encapsulated in microparticles using a spray-drying technique under optimized conditions: 170 °C temperature, 35 m3/h aspiration volume flow, and 7.5 mL/min feed flow. Arabic gum and maltodextrin were used as coating polymers individually in varying concentrations from 5 to 20% (w/v) and in combination. The microparticles were studied for morphology, particle size, oil content, and flowability. The formulated powder particles showed a high yield of 71 to 84%, mean diameter 2.41 to 5.99 µm, and total oil content of up to 10.80%. The results showed that both the wall material type and concentration, as well as the type of essential oil, significantly affected the encapsulation process and the final particle characteristics. Our study has demonstrated that the encapsulation of lavender and peppermint oils in Arabic gum/maltodextrin microparticles by spray-drying represents a feasible approach for the conversion of liquids into solids regarding their further use in powder technology.

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Comparative Study on Microencapsulation of Lavender (Lavandula angustifolia Mill.) and Peppermint (Mentha piperita L.) Essential Oils via Spray-Drying Technique.

Autores: Bissera Pilicheva, Yordanka Uzunova, Plamen Katsarov | Ano: 2021 | Database: pubmed

Resumo: Essential oils have been studied for various applications, including for therapeutic purposes. There is extensive literature regarding their properties; however, their low stability limits their application. Generally, the microencapsulation of essential oils allows enhanced stability and enables the potential incorporation in solid dosage forms. Lavender and peppermint oils were encapsulated in microparticles using a spray-drying technique under optimized conditions: 170 °C temperature, 35 m3/h aspiration volume flow, and 7.5 mL/min feed flow. Arabic gum and maltodextrin were used as coating polymers individually in varying concentrations from 5 to 20% (w/v) and in combination. The microparticles were studied for morphology, particle size, oil content, and flowability. The formulated powder particles showed a high yield of 71 to 84%, mean diameter 2.41 to 5.99 µm, and total oil content of up to 10.80%. The results showed that both the wall material type and concentration, as well as the type of essential oil, significantly affected the encapsulation process and the final particle characteristics. Our study has demonstrated that the encapsulation of lavender and peppermint oils in Arabic gum/maltodextrin microparticles by spray-drying represents a feasible approach for the conversion of liquids into solids regarding their further use in powder technology.

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Antifibrogenic Influence of Mentha piperita L. Essential Oil against CCl4-Induced Liver Fibrosis in Rats.

Autores: Hanan A Ogaly, Nadia A Eltablawy, Reham M Abd-Elsalam | Ano: 2018 | Database: pubmed

Resumo: Essential oils of some aromatic plants provide an effective nonmedicinal option to control liver fibrosis. Mentha piperita L. essential oil (MPEO) have been reported to possess protective effects against hepatotoxicity. However, its effect against liver fibrosis remains unknown. The present study investigated the antifibrogenic potential of MPEO and its underlying mechanisms. Forty male rats divided into 4 groups were used: group 1 served as normal control, group 2 (liver fibrosis) received CCl4 (2.5 mL/kg, IP, twice weekly) for 8 weeks, group 3 concurrently received CCl4 plus MPEO (50 mg/kg, IP, daily, from the 3rd week), and group 4 received MPEO only. MPOE significantly improved the liver injury markers, lipid peroxidation (LPO), antioxidant capacity, CYP2E1 gene expressionand liver histology. Furthermore, MPOE ameliorated liver fibrosis as evidenced by the reduced expression of desmin, α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), and SMAD3 proteins. In addition, MPOE counteracted the p53 upregulation induced by CCl4 at both mRNA and protein levels. In conclusion, MPOE could effectively attenuate hepatic fibrosis mainly through improving the redox status, suppressing p53 and subsequently modulating TGF-β1 and SMAD3 protein expression. These data promote the use of MPOE as a promising approach in antifibrotic therapy.

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Chemical and Antimicrobial Characterization of Mentha piperita L. and Rosmarinus officinalis L. Essential Oils and In Vitro Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells.

Autores: Alina Dolghi, Dorina Coricovac, Stefania Dinu, Iulia Pinzaru, Cristina Adriana Dehelean, Cristina Grosu, Doina Chioran, Petru Eugen Merghes, Cristian Andrei Sarau | Ano: 2022 | Database: pubmed

Resumo: Colorectal cancer is one of the most frequently diagnosed forms of cancer, and the therapeutic solutions are frequently aggressive requiring improvements. Essential oils (EOs) are secondary metabolites of aromatic plants with important pharmacological properties that proved to be beneficial in multiple pathologies including cancer. Mentha piperita L. (M_EO) and Rosmarinus officinalis L. (R_EO) essential oils are well-known for their biological effects (antimicrobial, antioxidant, anti-inflammatory and cytotoxic in different cancer cells), but their potential as complementary treatment in colorectal cancer is underexplored. The aim of the present study was to investigate the M_EO and R_EO in terms of chemical composition, antioxidant, antimicrobial, and cytotoxic effects in a colorectal cancer cell line-HCT 116. The gas-chromatographic analysis revealed menthone and menthol, and eucalyptol, α-pinene and L-camphor as major compounds in M_EO and R_EO respectively. M_EO exhibited potent antimicrobial activity, moderate antioxidant activity and a low cytotoxic effect in HCT 116 cells. R_EO presented a significant cytotoxicity in colorectal cancer cells and a low antimicrobial effect. The cytotoxic effect on non-cancerous cell line HaCaT was not significant for both essential oils. These results may provide an experimental basis for further research concerning the potential use of M_EO and R_EO for anticancer treatment.

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Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi.

Autores: Nitin Chauhan, Anushree Malik, Satyawati Sharma, R C Dhiman | Ano: 2016 | Database: pubmed

Resumo: The larvicidal activity of Mentha piperita, Cymbopogan citratus (lemongrass), Eucalyptus globulus and Citrus sinensis (orange) essential oils and their combinations was evaluated against Musca domestica (housefly) and Anopheles stephensi (mosquitoes) through contact toxicity assay. Among all the tested essential oils/combinations, Me. piperita was found to be the most effective larvicidal agent against Mu. domestica and An. stephensi with LC50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, after 48 h. The results clearly highlighted that the addition of mentha oil to other oils (1:1 ratio) improved their larvicidal activity. The order of effectiveness of essential oils/combinations indicated that the pattern for An. stephensi follows the trend as mentha > mentha + lemongrass > lemongrass > mentha + eucalyptus > eucalyptus > mentha + orange > orange and for Mu. domestica as mentha > mentha + lemongrass > lemongrass > mentha + orange > orange > mentha + eucalyptus > eucalyptus. The images obtained from scanning electron microscopy (SEM) analysis indicated the toxic effect of Me. piperita as the treated larvae were observed to be dehydrated and deformed. This study demonstrates the effectiveness of tested essential oils/combinations against the larval stages of Mu. domestica and An. stephensi and has the potential for development of botanical formulations.

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Enhancing the Antimicrobial Effect of Ozone with Mentha piperita Essential Oil.

Autores: Alin-Daniel Floare, Ramona Dumitrescu, Vlad Tiberiu Alexa, Octavia Balean, Camelia Szuhanek, Diana Obistioiu, Ileana Cocan, Alina-Georgeta Neacsu, Iuliana Popescu, Aurora Doris Fratila, Atena Galuscan | Ano: 2023 | Database: pubmed

Resumo: This study aimed to obtain and analyse Mentha piperita essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time-dose relationships and time-effect correlations. Mentha piperita (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time-dose relationship and specific t-test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: S. aureus > P. aeruginosa > E. coli > C. albicans > S. mutans. For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: C. albicans > E. coli > P. aeruginosa > S. aureus > S. mutans. The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.

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Prophylactic and curative potential of peppermint oil against calcium oxalate kidney stones.

Autores: Ayesha Jamshed, Qaiser Jabeen | Ano: 2021 | Database: pubmed

Resumo: Mentha piperita L., a well-known traditional herb, constitutes essential oil as one of its important constituent, used for its flavor, aroma and therapeutic applications. Based on the antioxidant, antispasmodic and nephroprotective potential, the essential oil of Mentha piperita was evaluated for its preventive and curative effects against ethylene glycol induced urolithiasis. Peppermint oil (Mp.Eo) was evaluated for its antioxidant potential by DPPH method. Urolithiasis was developed in male rats by the administration of ammonium chloride and ethylene glycol in drinking water. Different doses of Mp.Eo (10, 30 and 50 mg/kg) and cystone, the standard antiurolithic drug (500 mg/kg), were given along with stone-inducing regimen in prophylactic model and after intoxication for the next fourteen days in curative model. Urine and serum were analyzed for various biochemical parameters. One representative kidney from each group was studied for changes in histological parameters. Mp.Eo was found to be effective against urolithiasis-associated changes including crystalluria, polyuria and acidic urine. Mp.Eo also neutralized the altered levels of urinary uric acid, magnesium, total protein, serum creatinine and serum BUN. The data obtained from the present study demonstrated the therapeutic importance of peppermint oil against urolithiasis.

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Comparative glandular trichome transcriptome-based gene characterization reveals reasons for differential (-)-menthol biosynthesis in Mentha species.

Autores: Md Qussen Akhtar, Nida Qamar, Pallavi Yadav, Pallavi Kulkarni, Ajay Kumar, Ajit Kumar Shasany | Ano: 2017 | Database: pubmed

Resumo: The genes involved in menthol biosynthesis are reported earlier in Mentha  piperita. But the information on these genes is not available in Mentha arvensis. To bridge the gap in knowledge on differential biosynthesis of monoterpenes leading to compositional variation in the essential oil of these species, a comparative transcriptome analysis of the glandular trichome (GT) was carried out. In addition to the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathway genes, about 210 and 196 different terpene synthases (TPSs) transcripts were identified from annotation in M. arvensis and M.  piperita, respectively, and correlated to several monoterpenes present in the essential oil. Six isoforms of (-)-menthol dehydrogenases (MD), the last enzyme of the menthol biosynthetic pathway, were identified, cloned and characterized from the transcriptome data (three from each species). Varied expression levels and differential enzyme kinetics of these isoforms indicated the nature and composition of the product, as these isoforms generate both (-)-menthol and (+)-neomenthol from (-)-menthone and converts (-)-menthol to (-)-menthone in the reverse reaction, and hence together determine the quantity of (-)-menthol in the essential oil in these two species. Several genes for high value minor monoterpenes could also be identified from the transcriptome data.

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Dual Utilization of Medicinal and Aromatic Crops as Bioenergy Feedstocks.

Autores: Valtcho D Zheljazkov, Charles Neal Stewart, Blake Joyce, Holly Baxter, Charles L Cantrell, Tess Astatkie, Ekaterina A Jeliazkova, Charleson R Poovaiah | Ano: 2018 | Database: pubmed

Resumo: Dual production of biofuels and chemicals can increase the economic value of lignocellulosic bioenergy feedstocks. We compared the bioenergy potential of several essential oil (EO) crops with switchgrass ( Panicum virgatum L.), a crop chosen to benchmark biomass and lignocellulosic biofuel production. The EO crops of interest were peppermint ( Mentha piperita L.), "Scotch" spearmint ( Mentha gracilis Sole), Japanese cornmint ( Mentha canadensis L.), and sweet sagewort ( Artemisia annua L.). We also assessed each crop for EO production in a marginal production environment in Wyoming, USA, with irrigation and nitrogen (N) rates using a split-plot experimental design. Oil content ranged from 0.31 to 0.4% for Japanese cornmint, 0.23 to 0.26% for peppermint, 0.38 to 0.5% for spearmint, and the overall mean of sweet sagewort was 0.34%. Oil yields ranged from (in kg ha-1) 34 to 165 in Japanese cornmint, 25 to 108 in peppermint, 29.3 to 126 in spearmint, and 39.7 in sweet sagewort. EO production, but not composition, was sensitive to N fertilization. The alternative bioenergy crops and switchgrass produced similar amounts of ethanol from bench-scale simultaneous saccharification and fermentation assays. Value-added incomes from the EO proceeds were estimated to be between $1055 and $5132 ha-1 from peppermint, $1309 and $5580 ha-1 from spearmint, $510 and $2460 ha-1 from Japanese cornmint, and $3613 ha-1 from sweet sagewort under Wyoming growth conditions. The advantage of the proposed crops over traditional lignocellulosic species is the production of high-value natural products in addition to lignocellulosic biofuel production.

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Gamma irradiation-induced variability in morpho-agronomic and oil quality traits of Mentha piperita L.

Autores: Priyanka Prasad, Vagmi Singh, Nashra Aftab, Akancha Gupta, Ram Kishor, Himanshu Kumar Kushwaha, Vivek Singh, Ram Swaroop Verma, Birendra Kumar | Ano: 2021 | Database: pubmed

Resumo: To develop elite mutant in Mentha piperita having morphotype and oil content of Mentha arvensis, and oil quality of M. piperita.

Resultados: The developed and selected superior mutants viz. MPK-5(1) and MPK-5(3) have a mean herb yield potential of 23.923 and 21.503 Kg/9 m2; mean oil yield of 92.953 and 80.047 ml/plot; mean menthol content of 69.012% and 69.160% with mean menthofuran content of 1.554% and 0.531%, respectively.

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Mycorrhizal fungi inoculation and phosphorus fertilizer on growth, essential oil production and nutrient uptake in peppermint (Mentha piperita L. )/ Inoculação com fungos micorrízicos e adubação fosfatada no crescimento, produção de óleo essencial e absorção de nutrientes em hortelã-pimenta (Mentha piperita L. )

Autores: Arango, M. C; Ruscitti, M. F; Ronco, M. G; Beltrano, J | Ano: 2012 | Database: LILACS

Resumo: This study evaluated the effects of inoculation with the arbuscular mycorrhizal fungi Glomus mosseae, Glomus intraradices A4 and Glomus intraradices B1 and two phosphorus levels (10 and 40 mg kg-1) on root colonization, plant growth, nutrient uptake and essential oil content in Mentha piperita L. The experiment was carried out in a greenhouse, in 4x2 factorial arrangement, in completely randomized design. At sixty days after transplanting, the mycorrhizal plants had significantly higher fresh matter, dry matter and leaf area compared to non-mycorrhizal plants. The inoculation increased P, K and Ca levels in the shoot which were higher under 40 mg P kg-1 of soil. Plants grown with 40 mg P kg-1 soil increased the essential oil yield per plant by about 40-50% compared to those cultivated with 10 mg P kg-1, regardless of the mycorrhizal treatment. Among the studied fungal species, inoculation with G. intraradices A4 and a high level of P significantly increased plant growth and essential oil yield, compared to the other studied mycorrhizal fungal species. In conclusion, inoculation of arbuscular mycorrhizal fungi into peppermint plants is a feasible alternative to increase the essential oil production and reduce the use of fertilizers required to obtain economic production of peppermint under phosphorus-deficient soil condition.

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Toxicity and Physiological Effects of Nine Lamiaceae Essential Oils and Their Major Compounds on Reticulitermes dabieshanensis.

Autores: Xi Yang, Chunzhe Jin, Ziwei Wu, Hui Han, Zhilin Zhang, Yongjian Xie, Dayu Zhang | Ano: 2023 | Database: pubmed

Resumo: The volatile metabolites of Salvia sclarea, Rosmarinus officinalis, Thymus serpyllum, Mentha spicata, Melissa officinalis, Origanum majorana, Mentha piperita, Ocimum basilicum and Lavandula angustifolia were determined by gas chromatography-mass spectrometry. The vapor insecticidal properties of the analyzed essential oils and their compounds were screened using Reticulitermes dabieshanensis workers. The most effective oils were S. sclarea (major constituent linalyl acetate, 65.93%), R. officinalis (1,8-cineole, 45.56%), T. serpyllum (thymol, 33.59%), M. spicata (carvone, 58.68%), M. officinalis (citronellal, 36.99%), O. majorana (1,8-cineole, 62.29%), M. piperita (menthol, 46.04%), O. basilicum (eugenol, 71.08%) and L. angustifolia (linalool, 39.58%), which exhibited LC50 values ranging from 0.036 to 1.670 μL/L. The lowest LC50 values were recorded for eugenol (0.060 μL/L), followed by thymol (0.062 μL/L), carvone (0.074 μL/L), menthol (0.242 μL/L), linalool (0.250 μL/L), citronellal (0.330 μL/L), linalyl acetate (0.712 μL/L) and 1,8-cineole (1.478 μL/L). The increased activity of esterases (ESTs) and glutathione S-transferase (GST) were observed but only alongside the decreased activity of acetylcholinesterase (AChE) in eight main components. Our results indicate that S. sclarea, R. officinalis, T. serpyllum, M. spicata, M. officinalis, O. marjorana, M. piperita, O. basilicum and L. angustifolia essential oils (EOs) and their compounds, linalyl acetate, 1,8-cineole, thymol, carvone, citronellal, menthol, eugenol and linalool could be developed as control agents against termites.

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Nutritional and sensory quality during refrigerated storage of fresh-cut mints (Menthapiperita and M. spicata).

Autores: Ana Curutchet, Eduardo Dellacassa, Jorge A Ringuelet, Alicia R Chaves, Sonia Z Viña | Ano: 2014 | Database: pubmed

Resumo: The effect of storage time on quality attributes of refrigerated fresh-cut mints (Menthapiperita and M. spicata) was studied. Atmosphere composition, respiratory activity, weight loss, surface colour, total chlorophyll, carotenoids, browning potential, total phenols, flavonoids, radical-scavenging activity, ascorbic acid and essential oil yield and composition were analysed. Respiratory activity of peppermint and spearmint samples diminished moderately (42% and 28%, respectively) after 21days at 0°C. A slight modification of the internal atmosphere was achieved. Surface colour, chlorophyll, carotenoid and antioxidant compounds remained almost constant. The yield of essential oil did not change or it showed an apparent increase after 21days at 0°C, depending on plant growth stage. The characteristic flavour components of peppermint (menthone and menthol) increased, while the contents of the main constituents of spearmint essential oil showed minor variations after storage. The conditions assayed for packaging and storing fresh-cut mints were adequate to achieve a relatively long shelf life and they retained their antioxidant properties.

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Peppermint Essential Oil for Nausea and Vomiting in Hospitalized Patients: Incorporating Holistic Patient Decision Making Into the Research Design.

Autores: Carla Mohr, Cassandra Jensen, Nicole Padden, Jamie M Besel, Jeannine M Brant | Ano: 2021 | Database: pubmed

Resumo: This study examined nausea and vomiting (N/V) in hospitalized patients following the use of inhaled peppermint essential oil (aromatherapy) compared to combined aromatherapy/antiemetics or antiemetics alone.

Resultados: Only three subjects enrolled in the antiemetic arm; thus this arm was eliminated from analysis, resulting in 100 evaluable patients. Mean nausea score improved significantly for the entire sample following the aromatherapy or aromatherapy/antiemetic intervention (p < .0001). Patients in the aromatherapy arm had significant improvement in nausea compared to the combined aromatherapy/antiemetic arm (p < .0001). Patient perception that peppermint oil relieves N/V significantly improved for the entire sample. Notable is that 65% of patients used peppermint essential oil alone.

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Evaluation of Four Plant Extract Repellents for Management of the European Red Ant Myrmica rubra (Hymenoptera: Formicidae).

Autores: Kerry Bernard, Eleanor Groden, Francis A Drummond | Ano: 2020 | Database: pubmed

Resumo: Spearmint oil, peppermint oil, neem oil, and d-limonene were tested as nest site repellents against the colonization of the invasive European red ant, Myrmica rubra (L.) in both laboratory and field trials. In a laboratory assay, a 10% (v/v) solution of each extract repelled M. rubra colonies from nesting in plant pots filled with moist soil compared to water-treated controls, when applied as a dip to pots. Extracts also repelled colonies compared to a water control in a second laboratory experiment, where pots were dipped 15 d prior to the start of the experiment. In a 2008 field comparison, 20 plant pots, filled with moist potting soil, were dipped in either 10% (v/v) spearmint oil or peppermint oil, 70% (v/v) neem oil, or a water control and left in infested sites. Seven control pots were colonized over 3 mo, while none of the extract-treated pots were colonized. This field trial was repeated for a 15-wk duration in 2009 with the addition of a 10% (v/v) d-limonene solution as a treatment, and a change in neem oil concentration to 10% (v/v). Spearmint and peppermint oils repelled M. rubra colonies for the duration of the experiment. Neem oil and d-limonene repelled colonies for 3 and 4 wk, respectively. These extracts, especially mint oils, show potential as low-hazard repellents against M. rubra in greenhouse and nursery settings, and could reduce the number of new infestations incurred by the transport of plant stock.

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Repellency potential of essential oils against housefly, Musca domestica L.

Autores: Nitin Chauhan, Anushree Malik, Satyawati Sharma | Ano: 2018 | Database: pubmed

Resumo: In present study, the essential oils such as Mentha piperita (mentha oil, M.O), Cymbopogan citratus (lemongrass oil, LG.O), Citrus sinensis (orange oil, O.O), and Eucalyptus globulus (eucalyptus oil, E.O) were evaluated for repellency against housefly (Musca domestica) in a specially designed chamber. Further, to study any synergistic effect, essential oil combinations, i.e., M.O + LG.O, M.O + O.O, and M.O + E.O, were screened at 50:50 and 70:30 ratios. The results showed superior repellency of mentha and mentha + lemongrass (70:30) with RC95 value of 0.009 μl/cm3. The other oils and combinations showed higher values of RC95 (0.010-0.041 μl/cm3). The order of repellency was observed to be mentha = mentha + lemongrass (70:30) > mentha + lemongrass (50:50) = lemongrass = mentha + orange (50:50) = mentha + orange (70:30) > mentha + eucalyptus (70:30) > orange > mentha + eucalyptus (50:50) > eucalyptus. Chemical composition of selected essential oils indicated various monoterpenes as active components for efficient repellency. The essential oil of mentha marked the presence of menthol (38%) and menthone (27%) in major fractions, whereas citral (49%) was found dominating in lemongrass oil. Eucalyptus and orange oils showed the presence of 1,8-cineole (85%), and limonene (87%), respectively, as major components of oils. Further, monoterpenes (menthol and limonene) were also evaluated for repellency against housefly. The data showed 90 ± 5 and 60 ± 5% repellency from menthol and limonene, respectively, after 1 h, indicating the vital role of monoterpenes in overall efficacy of essential oil.

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Natural peppermint-flavored cheese.

Autores: Osama Abd El-Hamid Ibrahim, Ashraf Gaber Mohamed, Wafaa Kamel Bahgaat | Ano: 2019 | Database: pubmed

Resumo: The essential oils of edible and medicinal plants, herbs and spices are natural biologically ac- tive agents. Peppermint has a special position in Egyptian culture and is used as fresh or dried leaves. One of the main aims of the food industry is to manufacture products with good sensory acceptance. The present study aims to produce a novel attractive cheese with a low concentration of natural peppermint (Mentha piperita) essential oil (PEO). Moreover, PEO volatile composition, total phenolic content (TPC) and antioxidant activity extracted by the hydro-distillation of peppermint fresh leaves were investigated.

Resultados: Identification of PEO’s volatile compounds using GC-MS with a flame ionization detector analy- sis showed that the main components were menthol (37.62%), menthone (20.98%), carvone (11.76%), dihydro carveol acetate (11.23%), cineol (5.89%), β-caryophyllene (2.94), limonene (2.78%) and iso-menthone (2.39). Moreover, the antioxidant activity of PEO was 56.03 (%) with a TPC of 0.299 (mg/ml). Sensory evaluation of cheese showed the flavor and body and texture of PEO-flavored cheeses were higher than plain cheese during the storage period. There were no significant (p ≤ 0.05) differences in the cheese’s appearance nor in the chemical composition of plain and PEO-flavored cheeses. However, the TVFA and SN of plain cheese was significantly (p ≤ 0.05) lower than PEO cheeses at the level of 60, 80 and 100 ppm during the storage period.

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Final report on the safety assessment of Mentha Piperita (Peppermint) Oil, Mentha Piperita (Peppermint) Leaf Extract, Mentha Piperita (Peppermint) Leaf, and Mentha Piperita (Peppermint) Leaf Water.

Autores: B Nair | Ano: 2001 | Database: pubmed

Resumo: Mentha Piperita (Peppermint) Oil, Mentha Piperita (Peppermint) Leaf Extract, Mentha Piperita (Peppermint) Leaf, Mentha Piperita (Peppermint) Leaf Water are obtained from the Mentha piperita plant. The oil is currently used in cosmetic formulations as a fragrance component, but previously had been also described as a denaturant. The extract and leaves are described as biological additives, but only the extract is reported to be used. Peppermint Water is described as a flavoring agent or fragrance component, but is not currently in use. Peppermint Oil is used at a concentration of < or = 3% in rinse-off formulations and < or = 0.2% in leave-on formulations. Peppermint Oil is composed primarily of menthol and menthone. Other possible constituents include pulegone, menthofuran, and limone. Most of the safety test data concern Peppermint Oil. The oil is considered to present the "worst case scenario" because of its many constituents, so data on the oil were considered relevant to the entire group of ingredients. Peppermint Oil was minimally toxic in acute oral studies. Short-term and sub-chronic oral studies reported cystlike lesions in the cerebellum in rats that were given doses of Peppermint Oil containing pulegone, pulegone alone, or large amounts (>200 mg/kg/day) of menthone. Pulegone is also a recognized hepatotoxin. Repeated intradermal dosing with Peppermint Oil produced moderate and severe reactions in rabbits, although Peppermint Oil did not appear to be phototoxic. Peppermint Oil was negative in the Ames test and a mouse lymphoma mutagenesis assay but gave equivocal results in a Chinese hamster fibroblast cell chromosome aberration assay. In a carcinogenicity study of toothpaste and its components, no apparent differences were noted between mice treated with Peppermint Oil and those treated with the toothpaste base. Isolated clinical cases of irritation and/or sensitization to Peppermint Oil and/or its constituents have been reported, but Peppermint Oil (8%) was not a sensitizer when tested using a maximization protocol. It was expected that dermal absorption of Peppermint Oil would be rapid, following that of menthol, a major component, but in no case would be greater than absorption through the gastrointestinal tract. Because of the toxicity of pulegone, the safe concentration of this constituent was limited to < or = 1%. This concentration was achievable both by controlling the time of harvest and processing technique. There is evidence that menthol can enhance penetration of other agents. Formulators were cautioned that this enhanced penetration can affect the use of other ingredients whose safety assessment was based on their lack of absorption. With the limitation that the concentration of pulegone in these ingredients should not exceed 1%, it was concluded that Mentha Piperita (Peppermint) Oil, Mentha Piperita (Peppermint) Extract, Mentha Piperita (Peppermint) Leaves, Mentha Piperita (Peppermint) Water are safe as used in cosmetic formulations.

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Comparative glandular trichome transcriptome-based gene characterization reveals reasons for differential (-)-menthol biosynthesis in Mentha species.

Autores: Md Qussen Akhtar, Nida Qamar, Pallavi Yadav, Pallavi Kulkarni, Ajay Kumar, Ajit Kumar Shasany | Ano: 2017 | Database: pubmed

Resumo: The genes involved in menthol biosynthesis are reported earlier in Mentha  piperita. But the information on these genes is not available in Mentha arvensis. To bridge the gap in knowledge on differential biosynthesis of monoterpenes leading to compositional variation in the essential oil of these species, a comparative transcriptome analysis of the glandular trichome (GT) was carried out. In addition to the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathway genes, about 210 and 196 different terpene synthases (TPSs) transcripts were identified from annotation in M. arvensis and M.  piperita, respectively, and correlated to several monoterpenes present in the essential oil. Six isoforms of (-)-menthol dehydrogenases (MD), the last enzyme of the menthol biosynthetic pathway, were identified, cloned and characterized from the transcriptome data (three from each species). Varied expression levels and differential enzyme kinetics of these isoforms indicated the nature and composition of the product, as these isoforms generate both (-)-menthol and (+)-neomenthol from (-)-menthone and converts (-)-menthol to (-)-menthone in the reverse reaction, and hence together determine the quantity of (-)-menthol in the essential oil in these two species. Several genes for high value minor monoterpenes could also be identified from the transcriptome data.

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Peppermint Essential Oil Toxicity to the Pear Psylla (Hemiptera: Psyllidae) and Potential Applications in the Field.

Autores: Jianyi Li, Baoliang Tian | Ano: 2020 | Database: pubmed

Resumo: Chinese pear psylla (Cacopsylla chinensis Yang et Li) is a serious orchard pest that causes declines in fruit quality through feeding damage and the spread of pathogens. The rapid development of chemical pesticide resistance has become a severe problem in controlling pear psylla. Thus, the development of natural pesticides to replace conventional chemical pesticides is urgently needed. Here, we found that the essential oil of peppermint (Mentha haplocalyx Briq. [Lamiales: Labiatae]) is an ideal agent for controlling pear psylla based on experiments in the laboratory and the field. The major constituents of peppermint essential oil were found including menthol (49.73%), menthone (30.52%), α-pinene (3.60%), and α-terpineol (3.81%). This oil and chemicals in it performed serious contact toxicity against the winter-form adults and nymphs of pear psylla, yielding LD50 values of 2.54, 10.71, 2.77, 5.85, and 12.58 μg/adult and 1.91, 9.56, 2.18, 4.98, and 12.07 μg/nymph, respectively. Furthermore, the essential oil strongly repelled the adults of pear psylla with 78% repellence at the highest concentration tested in a Y-tube olfactometer in the laboratory. The combined effect of the two factors made peppermint essential oil a natural pesticide, which achieved a maximum reduction of round to 80.9% in winter-form adult population and round to 67.0% in nymph population at the concentration of 4.0 ml/L in the field. Additionally, it had no effect on the natural enemies of pear psylla in the field. Therefore, peppermint essential oil has potential as an alternative to chemical pesticides for pest control in integrated pest management programs in pear orchards.

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Lethal and Sublethal Effects of Eucalyptus camaldulensis and Mentha piperita Essential Oils on the Khapra Beetle (Coleoptera: Dermestidae) in Terms of Feeding Inhibition, Oviposition, and Seed Damage.

Autores: Ehsan Borzoui, Ramin Khaghani, Gadir Nouri-Ganbalani | Ano: 2021 | Database: pubmed

Resumo: Trogoderma granarium Everts, the Khapra beetle, is a major pest of stored products, especially grains. In this study, fumigant toxicity and sublethal effects of Eucalyptus camaldulensis Dehnh. (Myrtaceae) and Mentha piperita L. (Lamiaceae) essential oils (EOs) were investigated against different growth stages of T. granarium. To assess the sublethal effects, insects were exposed to an LC20 or LC50 concentration of each essential oil, and the ability of these oils to deter feeding, oviposition, and damage to wheat seeds and overall mass were surveyed. At LC50 concentrations, M. piperita EO showed higher fumigant toxicity than E. camaldulensis EO against eggs, 2nd instar larvae, 4th instar larvae, and adults of T. granarium. Furthermore, the adults were more sensitive to the tested EOs than immatures. In free-choice tests, both larvae and adults showed a preference for control-treated wheat seeds than for seeds treated with an LC20 or LC50 concentration of EOs from E. camaldulensis or M. piperita. In a no-choice test, adult females exposed to EOs showed lower fecundity and fertility in comparison to control females not exposed to EOs. Treatment of wheat seeds with E. camaldulensis or M. piperita EOs resulted in a dose-specific reduction in the number of damaged seeds and seed weight loss when compared to control. According to our results, both tested EOs, especially EOs extracted from M. piperita, showed good potential for use in integrated pest management strategies against T. granarium.

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Toxicity and repellent activity of essential oil from Mentha piperita Linn. leaves and its major monoterpenoids against three stored product insects.

Autores: Xue Pang, Yi-Xi Feng, Xiao-Jie Qi, Yang Wang, Borjigidai Almaz, Chao Xi, Shu-Shan Du | Ano: 2020 | Database: pubmed

Resumo: The essential oil (EO) from leaves of Mentha piperita was extracted by hydrodistillation. Twenty-one chemical components, accounting for 97.5% of the total oil, were determined by GC-MS and GC-FID. The major chemical components included menthol (41.6%), L-menthone (24.7%), isomenthol (6.3%), and limonene (5.0%). The bioactivity of the obtained EO and its two major components against Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults were evaluated by fumigation, contact, and repellent activity bioassay. The EO showed significant fumigation and contact toxicity against T. castaneum (LC50 = 18.1 mg/L air and LD50 = 2.9 μg/adult, respectively), L. serricorne (LC50 = 68.4 mg/L air and LD50 = 12.6 μg/adult, respectively), and L. bostrychophila (LC50 = 0.6 mg/L air and LD50 = 49.8 μg/adult, respectively) adults. Meanwhile, the repellent effect of the EO on T. castaneum and L. serricorne adults was comparable to that of the positive control at the highest tested concentration. Menthol and L-menthone were two major components in total oil. Among them, L-menthone exhibited significant insecticidal activity on target insects, and menthol showed notable repellent effects. The results indicated that the EO of M. piperita leaves and two tested components have potential to be developed as natural insecticides and repellents for the control of stored product insect pests. Graphical abstract.

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Chemical Composition and Insecticidal Activities of Essential Oils against the Pulse Beetle.

Autores: C S Jayaram, Nandita Chauhan, Shudh Kirti Dolma, S G Eswara Reddy | Ano: 2022 | Database: pubmed

Resumo: Pulse beetles, Callosobruchus chinensis and Callosobruchus maculatus, are essential pests of cowpea, gram, soybean and pulses. Application of synthetic insecticides against the pulse beetle has led to insect resistance; insecticide residues on grains affect human health and the environment. Essential oils (EOs) are the best alternatives to synthetics due to their safety to the environment and health. The main objective of the investigation was to study the chemical composition and insecticidal activities of EOs, their combinations and compounds against the pulse beetle under laboratory. Neo-isomenthol, carvone and β-ocimene are the significant components of tested oils using GC-MS. Mentha spicata showed promising fumigant toxicity against C. chinensis (LC50 = 0.94 µL/mL) and was followed by M. piperita (LC50 = 0.98 µL/mL), whereas M. piperita (LC50 = 0.92 µL/mL) against C. maculatus. A combination of Tagetes minuta + M. piperita showed more toxicity against C. chinensis after 48 h (LC50 = 0.87 µL/mL) than T. minuta + M. spicata (LC50 = 1.07 µL/mL). L-Carvone showed fumigant toxicity against C. chinensis after 48 h (LC50 = 1.19 µL/mL). Binary mixtures of T. minuta +M. piperita and M. spicata showed promising toxicity and synergistic activity. EOs also exhibited repellence and ovipositional inhibition. The application of M. piperita can be recommended for the control of the pulse beetle.

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In vitro study on the transfer of volatile oil components.

Autores: K Szentmihályi, E Forgács, M Hajdú, M Then | Ano: 2001 | Database: pubmed

Resumo: The following volatile oils were tested in vitro: chamomile (Matricaria recutica L.), peppermint (Mentha piperita L.) and sage (Salvia officinalis L.) to obtain information on which components of volatile oils or minerals are able to pass through the membranes under different conditions. The transfer of chamomile and peppermint oil from aqueous volatile oil to the stomach (pH=1.1) and then to the plasma (pH=7.5) was studied, and the transfer of sage oil through the skin (from pH=5.5 to pH=7.5) was examined. The transfer of some components was more favorable than that of others. The transfer of chamomile oil was faster to buffer pH=1.1 than from buffer pH=1.1 to buffer pH=7.5 and most of the components, except for chamazulene, passed through the membranes. In the case of peppermint the components went through the membranes in the first 15 min although the main components mostly remained in the initial solution. The sage oil transferred showed the same characteristics as the starting oil. A small amount of metal present in the volatile oils also passed through the membranes. The transfer of metals varied, depending on the time, type of the oil, metal quality and the conditions applied.

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Inhalation of Essential Oil from Mentha piperita Ameliorates PM10-Exposed Asthma by Targeting IL-6/JAK2/STAT3 Pathway Based on a Network Pharmacological Analysis.

Autores: Mi Hye Kim, Sang Jun Park, Woong Mo Yang | Ano: 2020 | Database: pubmed

Resumo: Fine particulate matter (PM) exposure exhibits a crucial risk factor to exacerbate airway epithelial remodeling, fibrosis, and pulmonary destruction in asthma. Based on the use of essential oils from aromatic plants on pain relief and anti-inflammatory properties, we investigated the inhibitory effects of essential oil derived from the Mentha species (MEO) against asthma exposed to PM10. The MEO (0.1 v/v %) was aerosolized by a nebulizer to ovalbumin and PM10-induced asthmatic mice. Histological changes were confirmed in the lung tissues. To define the mode of action of the MEO on asthma, a protein-protein interaction network was constructed using menthol and menthone as the major components of the MEO. Cytokine expression and the JAK2/STAT3 signaling pathway were analyzed in lung epithelial A549 cells co-treated with MEO and PM10. Inhalation of MEO by nebulization inhibited respiratory epithelium hyperplasia, collagen deposition, and goblet cell activation in asthmatic mice. Through a network pharmacological analysis, cytokine-cytokine receptor interaction and JAK/STAT was expected to be underlying mechanisms of MEO on asthma. Treatment with MEO significantly reduced the IL-6 levels with a decrease in pro-inflammatory and T helper 2-specific cytokines. PM10-induced phosphorylation of JAK2 and STAT3 was significantly decreased by MEO. Collectively, MEO may have an inhibitory effect on asthma under the condition of PM10 exposure through the IL-6/JAK2/STAT3 signaling pathway.

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Seasonal variability of the main components in essential oil of Mentha  piperita L.

Autores: Daniela Grulova, Laura De Martino, Emilia Mancini, Ivan Salamon, Vincenzo De Feo | Ano: 2015 | Database: pubmed

Resumo: Mentha piperita is an important and commonly used flavoring plant worldwide. Its constituents, primarily menthol and menthone, change in the essential oil depending on internal and external factors, of which environmental conditions appear very important. The experiment was established in 2010 for three vegetation season, in order to observe the quantitative changes of the main components of peppermint. The determination of menthol, menthone, limonene, menthyl acetate, menthofuran and β-caryophyllene was registered.

Resultados: In the experimental season 2011 and 2012 a higher mean temperature than in 2010 and extreme rainfall in July 2011 and 2012 were recorded. Different environmental conditions affected the development of M. piperita plants and the content and composition of the essential oil.

Conclusão: Seasonal and maturity variations are interlinked with each other, because the specific ontogenic growth stage differed as the season progressed. Fluctuations in monthly and seasonal temperature and precipitation patterns affected the quality of peppermint essential oil.