GUALTÉRIA

Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies.

Autores: Somtirtha Banerjee, Biswajoy Bagchi, Kunal Pal, Suman Bhandary, Arpan Kool, Nur Amin Hoque, Prosenjit Biswas, Pradip Thakur, Kaustuv Das, Parimal Karmakar, Sukhen Das | Ano: 2020 | Database: pubmed

Resumo: In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet by vacuum filtration technique using Buckner funnel. Phase crystallization, fluorescence property and microstructure of eHAp were confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Photoluminiscence spectroscopy (PL) and Field emission scanning electron microscopy (FESEM). Strong antimicrobial activity was observed for EU, TTO and WO on both E. coli and S. aureus mediated by cell membrane damage and leakage of cytoplasmic components. The oil absorbed eHAp nanocomposites were found to be moderately biocompatible with normal WI-38 cells up to MIC concentration various time scale. The nanocomposites showed significant cytotoxic activity on breast cancer cell line MDA-MB 468 and the fluorescent property of the eHAp was utilized to visualize internalization of particles in the cells. The release profile of the oils from the eHAp matrix showed pH dependent release indicated that the porous matrix can be used as a suitable carrier for modulated and sustained release of bioactive components. Thus, given the multifunctional attributes these natural essential oil-based nanocomposites show great promise as an alternative to conventional therapeutic treatments.

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Conclusão: N/A

Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies.

Autores: Somtirtha Banerjee, Biswajoy Bagchi, Kunal Pal, Suman Bhandary, Arpan Kool, Nur Amin Hoque, Prosenjit Biswas, Pradip Thakur, Kaustuv Das, Parimal Karmakar, Sukhen Das | Ano: 2020 | Database: pubmed

Resumo: In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet by vacuum filtration technique using Buckner funnel. Phase crystallization, fluorescence property and microstructure of eHAp were confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Photoluminiscence spectroscopy (PL) and Field emission scanning electron microscopy (FESEM). Strong antimicrobial activity was observed for EU, TTO and WO on both E. coli and S. aureus mediated by cell membrane damage and leakage of cytoplasmic components. The oil absorbed eHAp nanocomposites were found to be moderately biocompatible with normal WI-38 cells up to MIC concentration various time scale. The nanocomposites showed significant cytotoxic activity on breast cancer cell line MDA-MB 468 and the fluorescent property of the eHAp was utilized to visualize internalization of particles in the cells. The release profile of the oils from the eHAp matrix showed pH dependent release indicated that the porous matrix can be used as a suitable carrier for modulated and sustained release of bioactive components. Thus, given the multifunctional attributes these natural essential oil-based nanocomposites show great promise as an alternative to conventional therapeutic treatments.

Resultados: N/A

Conclusão: N/A

Phytochemical characterization, genotoxicity, cytotoxicity, and antimicrobial activity of Gautheria procumbens essential oil.

Autores: Camila Marina Verdi, Vanessa Schopf Machado, Alencar Kolinsk Machado, Bruna Klein, Pauline Cordenonsi Bonez, Eduardo Nascimento Correa de Andrade, Grazielle Rossi, Marli Matiko Campos, Roger Wagner, Michele Rorato Sagrillo, Roberto Christ Vianna Santos | Ano: 2022 | Database: pubmed

Resumo: This study investigated the chemical constituents of Gaultheria procumbens essential oil and is the first to relate cytogenotoxicity with oxidative metabolism and antimicrobial activity. Chromatographic analysis of the essential oil showed methyl salicylate (99.96%) and linalool (0.04%) as the major compounds. The essential oil showed no signs of cytogenotoxicity at different concentrations (1.82 to 58.34 mg mL-1). Furthermore, G. procumbens essential oil and methyl salicylate were used to evaluate the minimal inhibitory concentrations (MIC) and minimal microbicidal concentrations (MMC). The results showed efficacy against several microorganisms, including Aeromonas caviae, Candida albicans, and Mycobacterium fortuitum with MIC values ranging from 1.82 to 3.64 mg mL-1 and MMC values ranging from 3.64 to 12.67 mg mL-1, which were confirmed by time-kill kinetics. Based on our results, the essential oil is a promising alternative to developing future formulations to treat infections caused by microorganisms.

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Conclusão: N/A

Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies.

Autores: Somtirtha Banerjee, Biswajoy Bagchi, Kunal Pal, Suman Bhandary, Arpan Kool, Nur Amin Hoque, Prosenjit Biswas, Pradip Thakur, Kaustuv Das, Parimal Karmakar, Sukhen Das | Ano: 2020 | Database: pubmed

Resumo: In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet by vacuum filtration technique using Buckner funnel. Phase crystallization, fluorescence property and microstructure of eHAp were confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Photoluminiscence spectroscopy (PL) and Field emission scanning electron microscopy (FESEM). Strong antimicrobial activity was observed for EU, TTO and WO on both E. coli and S. aureus mediated by cell membrane damage and leakage of cytoplasmic components. The oil absorbed eHAp nanocomposites were found to be moderately biocompatible with normal WI-38 cells up to MIC concentration various time scale. The nanocomposites showed significant cytotoxic activity on breast cancer cell line MDA-MB 468 and the fluorescent property of the eHAp was utilized to visualize internalization of particles in the cells. The release profile of the oils from the eHAp matrix showed pH dependent release indicated that the porous matrix can be used as a suitable carrier for modulated and sustained release of bioactive components. Thus, given the multifunctional attributes these natural essential oil-based nanocomposites show great promise as an alternative to conventional therapeutic treatments.

Resultados: N/A

Conclusão: N/A

Toxicity of twenty-two plant essential oils against pathogenic bacteria of vegetables and mushrooms.

Autores: Biljana Todorović, Ivana Potočnik, Emil Rekanović, Miloš Stepanović, Miroslav Kostić, Mihajlo Ristić, Svetlana Milijašević-Marčić | Ano: 2016 | Database: pubmed

Resumo: ASBTRACT Toxicity of twenty-two essential oils to three bacterial pathogens in different horticultural systems: Xanthomonas campestris pv. phaseoli (causing blight of bean), Clavibacter michiganensis subsp. michiganensis (bacterial wilt and canker of tomato), and Pseudomonas tolaasii (causal agent of bacterial brown blotch on cultivated mushrooms) was tested. Control of bacterial diseases is very difficult due to antibiotic resistance and ineffectiveness of chemical products, to that essential oils offer a promising alternative. Minimal inhibitory and bactericidal concentrations are determined by applying a single drop of oil onto the inner side of each plate cover in macrodilution assays. Among all tested substances, the strongest and broadest activity was shown by the oils of wintergreen (Gaultheria procumbens), oregano (Origanum vulgare), and lemongrass (Cymbopogon flexuosus. Carvacrol (64.0-75.8%) was the dominant component of oregano oils, while geranial (40.7%) and neral (26.7%) were the major constituents of lemongrass oil. Xanthomonas campestris pv. phaseoli was the most sensitive to plant essential oils, being susceptible to 19 oils, while 11 oils were bactericidal to the pathogen. Sixteen oils inhibited the growth of Clavibacter michiganensis subsp. michiganensis and seven oils showed bactericidal effects to the pathogen. The least sensitive species was Pseudomonas tolaasii as five oils inhibited bacterial growth and two oils were bactericidal. Wintergreen, oregano, and lemongrass oils should be formulated as potential biochemical bactericides against different horticultural pathogens.

Resultados: N/A

Conclusão: N/A

Toxicity of twenty-two plant essential oils against pathogenic bacteria of vegetables and mushrooms.

Autores: Biljana Todorović, Ivana Potočnik, Emil Rekanović, Miloš Stepanović, Miroslav Kostić, Mihajlo Ristić, Svetlana Milijašević-Marčić | Ano: 2016 | Database: pubmed

Resumo: ASBTRACT Toxicity of twenty-two essential oils to three bacterial pathogens in different horticultural systems: Xanthomonas campestris pv. phaseoli (causing blight of bean), Clavibacter michiganensis subsp. michiganensis (bacterial wilt and canker of tomato), and Pseudomonas tolaasii (causal agent of bacterial brown blotch on cultivated mushrooms) was tested. Control of bacterial diseases is very difficult due to antibiotic resistance and ineffectiveness of chemical products, to that essential oils offer a promising alternative. Minimal inhibitory and bactericidal concentrations are determined by applying a single drop of oil onto the inner side of each plate cover in macrodilution assays. Among all tested substances, the strongest and broadest activity was shown by the oils of wintergreen (Gaultheria procumbens), oregano (Origanum vulgare), and lemongrass (Cymbopogon flexuosus. Carvacrol (64.0-75.8%) was the dominant component of oregano oils, while geranial (40.7%) and neral (26.7%) were the major constituents of lemongrass oil. Xanthomonas campestris pv. phaseoli was the most sensitive to plant essential oils, being susceptible to 19 oils, while 11 oils were bactericidal to the pathogen. Sixteen oils inhibited the growth of Clavibacter michiganensis subsp. michiganensis and seven oils showed bactericidal effects to the pathogen. The least sensitive species was Pseudomonas tolaasii as five oils inhibited bacterial growth and two oils were bactericidal. Wintergreen, oregano, and lemongrass oils should be formulated as potential biochemical bactericides against different horticultural pathogens.

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Conclusão: N/A

An in vitro ULV olfactory bioassay method for testing the repellent activity of essential oils against moths.

Autores: Petros T Damos | Ano: 2018 | Database: pubmed

Resumo: A prototype olfactory device was developed and used for first time to study the bioactivity of Ultra Low Volumes (ULV) of three essential oilsagainst the moth pest Anarsia lineatella (Lepidoptera: Gelechiidae). Particle sizes calibration and standard ULV time-doses range tests were performed prior the olfactory bioassays. Three essential oils were tested Cymbopogon citratus (Lemon Grass), Gaultheria procumbens (Winter Grass) and Rosmarinus officinalis (Rosmarin) according to the proposed method. The most active oil was that of R. officinalis and moths expressed approximately 3-5 fold faster moving behavior (50% repellence response times to ULV, RT50: 20-30 min) compared to G. procumbens (RT50:74-79 min) and C. citratus (RT50:82-96 min). Apart from direct observed repellence, moths sprayed with ULV show clearly signs of knock down symptoms and high fatality in a period 15-60 min after their treatment especial in the case of R. officinalis. Longevity of female moths was significantly affected by the initial ULV application. Furthermore, choice test showed that essential oils significantly deterred oviposition in most cases. Considering the urgent need for alternative to conventional pesticides the current work may provide a framework of testing the bioactivity of bio rational compounds in the form of ULV and under Lab conditions.

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