LIMÃO GALEGO

Effect of Massage Therapy with Lime (Citrus Aurantifolia) Essential Oil on the Recovery of Delayed Onset of Muscle Soreness in Athletes.

Autores: Novita Sari Harahap, Nimrot Manalu, Nurhamidah Sari Siregar, Yetty Machrina | Ano: 2023 | Database: pubmed

Resumo: Physical exercise is a systematic exercise to increase muscle strength achieve goals such as improving the athlete's physical and preventing injury. Athletes must do is massage therapy to prevent muscle fatigue and pain due to physical exercise or during competition. The increase of lactic acid levels affects the maximum working ability of muscle fibers, decreases physical performance, and is fatigue in which the onset of pain. Lime essential oil as a topical oil contains high ester substances that have pharmacological effects such as natural analgesic effects to relieve pain.

Resultados: The results showed that there was a significant difference in the average levels of lactic acid (p = 0.000) and the athlete's pain intensity (p = 0.000) in the three groups, namely, the exercise group (E), the exercise and massage group (EM), and the exercise and massage with lime essential oil (EMC).

Conclusão: This study finds that lime essential oil used as topical massage therapy oil is better for accelerating the DOMS.

Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman.

Autores: Maha S Al-Aamri, Nour M Al-Abousi, Sausan S Al-Jabri, Tanveer Alam, Shah A Khan | Ano: 2018 | Database: pubmed

Resumo: This study investigated the chemical composition and the antioxidant and antimicrobial activities of the essential oil isolated from the aerial parts of Citrus aurantifolia L.

Resultados: Thirty-three chemical compounds were identified, with d-limonene (63.35%) forming the major constituent. Other prominent constituents include 3,7-dimethyl-2,6-octadien-1-ol (7.07%), geraniol (6.23%), E-citral (4.35%), Z-citral (3.29%), and β-ocimene (2.25%). The essential oil of Citrus aurantifolia L. leaves showed excellent antibacterial activity against Staphylococcus aureus and moderate activity against pathogenic Escherichia coli strains. The oil exhibited promising in-vitro antioxidant activity (IC50 value = 21.57 μg/mL) but showed moderate antibacterial activities.

Conclusão: N/A

Hexosomal Dispersion: A Nano-Based Approach to Boost the Antifungal Potential of Citrus Essential Oils against Plant Fungal Pathogens.

Autores: Mohamed S Sedeek, Abdulaziz M Al-Mahallawi, Rania A A Hussien, Ahmed M Abdelhaleem Ali, Ibrahim A Naguib, Mai K Mansour | Ano: 2021 | Database: pubmed

Resumo: The demand for natural fungicides to replace synthetic ones has surged since toxic residues persist in soils, causing environmental contamination and posing a serious threat to worldwide public health. In the context of crop protection and enhancing the efficiency and safety of fungicides, nanotechnology is an eco-friendly strategy in managing fungal pathogens. In the present study, essential oils were isolated from the peels of four citrus fruits (Citrus lemon, Citrus aurantifolia, Citrus maxima, and Citrus sinensis) and were investigated using gas chromatography-mass spectrometric analysis. Monoterpene hydrocarbon was the most predominant group and limonene was the most abundant in the four oils. The antifungal potential of the oils was investigated, and the most active oil (Citrus lemon) was loaded into hexosomal dispersion, and its antifungal potential was retested against the same fungi. The structurally unique nano-based formulation showed great potency for fungal control. To the best of our knowledge, it is the first time the oil of Citrus lemon in nano-hexosomes has been formulated and its fungicidal activity examined. The data collected suggest that citrus essential oils (CEOs), especially when nano-formulated, could be successfully used in integrated fungus management programs.

Resultados: N/A

Conclusão: N/A

Eradication of C. albicans and T. rubrum with photoactivated indocyanine green, Citrus aurantifolia essential oil and fluconazole.

Autores: Reza Fekrazad, Arash Poorsattar Bejeh Mir, Vadood Ghasemi Barghi, Masoomeh Shams-Ghahfarokhi | Ano: 2015 | Database: pubmed

Resumo: We aimed to evaluate the efficacy of alternative therapies rather than the current antifungal conventional therapy and with assessing the hypothesis of photoactivation of citrus essential oil, fluconazole and Indocyanine green to treat two common mucocutaneous fungal infections.

Resultados: Cell reduction rates (%) in C. albicans groups were 99.99 (CE), 91.67 (IRLE), 86.67 (CC), 72.37 (E) and 67.27 (RL). Whereas, a 99.99 (CE), 89.99 (CC), 74.5 (IRLE), 64.5 (E), 38.5 (IRLF), 37.5 (RL), and 31 (FL) percent eradication was achieved in T. rubrum groups.

Conclusão: Photoactivation of Citrus EO increased the killing capability by 10-13%. A modest 7.5% augmented effect was observed with IR activation of Fluconazole. Both Citrus EO and photothermal-photodynamic therapy with ICG and IR diode laser exhibited remarkable lethal effect on fungal cells. Candida viable cells are more susceptible to laser only and ICG only treatments than Tricophyton cells.

Efeito inibitório de óleos essenciais do gênero Citrus sobre o crescimento de micro-organismos/ Inhibitory effect of essential oils from the genus Citrus on the microorganisms growth

Autores: Pires, Tamara Cubiaki; Piccoli, Roberta Hilsdorf | Ano: 2012 | Database: LILACS

Resumo: Os óleos essenciais encontrados em plantas medicinais, aromáticas e condimentares têm interessado às indústrias alimentícias, pelas suas propriedades antimicrobianas contra bactérias deteriorantes e/ou patogênicas. No intuito de avaliar o efeito inibitório dos óleos essenciais da casca e da folha de Citruslimonia Osbeck (limão-cravo), Citrus aurantifolia (Chrst.) Swingle (limão-galego) e Citrus latifolia Tanaka(limão-tahiti), foram realizados experimentos, utilizando-se a técnica de cultura de difusão em placa de ágar com as bactérias Staphylococcus aureus, Escherichia coli e Pseudomonas aeruginosa e levedura Candida utilis. Para o fungo Penicillium expansum, foram realizadas culturas em placas-padrão com e sem a presença dos óleos. Nos ensaios in vitro, os óleos essenciais testados promoveram inibição de todos os micro-organismos testados, e os maiores halos ocorreram sobre a levedura C. utilis. Nas bactérias, os óleos induziram melhor inibição sobre S. aureus do que em E. coli e P. aeruginosa. O efeito inibitório dos óleos de citros foi eficaz sobre o fungo P. expansum, exceto o óleo de limão-cravo, que mostrou efeito estimulante de crescimento. Os óleos essenciais analisados demonstraram efeito inibitório sobre os microorganismos avaliados.

Resultados: N/A

Conclusão: N/A

Lime (Citrus aurantifolia (Christm.) Swingle) Essential Oils: Volatile Compounds, Antioxidant Capacity, and Hypolipidemic Effect.

Autores: Li-Yun Lin, Cheng-Hung Chuang, Hsin-Chun Chen, Kai-Min Yang | Ano: 2019 | Database: pubmed

Resumo: Lime peels are mainly obtained from the byproducts of the juice manufacturing industry, which we obtained and used to extract essential oil (2.3%) in order to examine the antioxidant and hypolipidaemic effects. We identified 60 volatile compounds of lime essential oil (LEO) with GC/MS, of which the predominant constituents were limonene, γ-terpinene, and β-pinene. Lime essential oil was measured according to the DPPH assay and ABTS assay, with IC50 values of 2.36 mg/mL and 0.26 mg/mL, respectively. This study also explored the protective effects of LEO against lipid-induced hyperlipidemia in a rat model. Two groups of rats received oral LEO in doses of 0.74 g/100 g and 2.23 g/100 g with their diets. Eight weeks later, we found that the administration of LEO improved the serum total cholesterol, triglyceride, low-density lipoprotein cholesterol, alanine aminotransferase, and aspartate transaminase levels in the hyperlipidemic rats (p < 0.05). Simultaneously, the LEO improved the health of the rats in terms of obesity, atherogenic index, and fatty liver.

Resultados: N/A

Conclusão: N/A

Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus.

Autores: Luis Eduardo Ferreira, Bruno Iglesias Benincasa, Ana Lúcia Fachin, Silvia Helena Taleb Contini, Suzelei Castro França, Ana Carolina Souza Chagas, Rene Oliveira Beleboni | Ano: 2018 | Database: pubmed

Resumo: Infections of sheep with gastrointestinal parasites, especially Haemonchus contortus, have caused serious losses in livestock production, particularly after the emergence of resistance to conventional anthelmintics. The search for new anthelmintic agents, especially those of botanical origin, has grown substantially due to the perspective of less contamination of meat and milk, as well as other advantages related to their cost and accessibility in less developed countries. The aim of this study was to evaluate the in vitro anthelmintic activity of essential oils of the plant species Citrus aurantifolia, Anthemis nobile and Lavandula officinalis against the main developmental stages of the parasite H. contortus.

Resultados: Plant species were selected based on substantial ethnopharmacological information. Analysis of the composition of each oil by gas chromatography coupled to mass spectrometry (GC-MS) demonstrated the presence of limonene (56.37%), isobutyl angelate (29.26%) and linalool acetate (35.97%) as the major constituents in C. aurantifolia, A. nobile and L. officinalis, respectively. Different concentrations of each oil were tested in vitro for their capacity to inhibit egg hatching (EHT), larval development (LDT) and adult worm motility (AWMT) using a multidrug-resistant strain of H. contortus (Embrapa 2010). The IC50 values obtained for the oils of C. aurantifolia, A. nobile and L. officinalis were 0.694, 0.842 and 0.316 mg/ml in the EHT and 0.044, 0.117 and 0.280 mg/ml in the LDT, respectively. The three oils were able to inhibit adult worm motility completely within the first 8-12 h of observation in the AWMT.

Conclusão: N/A

Microencapsulation of lime (Citrus aurantifolia) oil for antibacterial finishing of cotton fabric.

Autores: Euis Julaeha, Sandra Puspita, Diana Rakhmawaty Eddy, Tatang Wahyudi, Mohamad Nurzaman, Jakariya Nugraha, Tati Herlina, Jamaludin Al Anshori | Ano: 2021 | Database: pubmed

Resumo: Functional cotton fabric incorporated with antibacterial microcapsules of lime (C. aurantifolia) essential oil (LO) was prepared. The coacervation method, employing two biopolymers of alginate and gelatin as the shells, was preferentially selected to produce the LO microcapsules, whereas immobilization of the LO microcapsules onto the fabric was done using the pad-dry-cure method using various concentrations of citric acid binder. The antibacterial inhibition zone of the functional fabric was subsequently analysed using the Kirby Bauer method. The LO microcapsules were produced with a yield, encapsulation efficiency (EE), and oil content (OC) of 47 ± 4%, 84 ± 11%, and 58 ± 4%, respectively. The homogenous spherical and soft microcapsules (1.554 μm) bonded effectively by 4% citric acid onto the surface of the fabric and detached back by only 3% after 15 cycles of washing. Overall, the optimized functional fabric exhibited the highest antibacterial activities among others against typical skin bacteria, such as S. aureus, E. coli, K. pneumoniae, and S. epidermidis, and thus it can be potentially applied to obtain antibacterial functional textile.

Resultados: N/A

Conclusão: N/A

Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman.

Autores: Maha S Al-Aamri, Nour M Al-Abousi, Sausan S Al-Jabri, Tanveer Alam, Shah A Khan | Ano: 2018 | Database: pubmed

Resumo: This study investigated the chemical composition and the antioxidant and antimicrobial activities of the essential oil isolated from the aerial parts of Citrus aurantifolia L.

Resultados: Thirty-three chemical compounds were identified, with d-limonene (63.35%) forming the major constituent. Other prominent constituents include 3,7-dimethyl-2,6-octadien-1-ol (7.07%), geraniol (6.23%), E-citral (4.35%), Z-citral (3.29%), and β-ocimene (2.25%). The essential oil of Citrus aurantifolia L. leaves showed excellent antibacterial activity against Staphylococcus aureus and moderate activity against pathogenic Escherichia coli strains. The oil exhibited promising in-vitro antioxidant activity (IC50 value = 21.57 μg/mL) but showed moderate antibacterial activities.

Conclusão: N/A

Chemical composition and antibacterial activity of essential oils from Citrus aurantifolia leaves and fruit peel against oral pathogenic bacteria.

Autores: Raiane S Lemes, Cassia C F Alves, Elisângela B B Estevam, Mariana B Santiago, Carlos H G Martins, Tainá C L Dos Santos, Antônio E M Crotti, Mayker L D Miranda | Ano: 2018 | Database: pubmed

Resumo: Tooth decay is a major public health problem which affects a large number of people in several countries. Even though more than 700 bacterial species have been detected in the oral cavity, Streptococcus and Lactobacillus stand out as the genera that cause tooth decay and other periodontal diseases. In this study, essential oils from Citrus aurantifolia leaves (CL-EO) and fruit peel (CP-EO) were obtained by hydrodistillation by a Clevenger-type apparatus whereas their chemical composition was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Limonene (77.5 %), linalool (20.1 %), citronellal (14.5 %) and citronellol (14.2 %) were the main constituents found in the essential oils from C. aurantifolia leaves and fruit peel. Antibacterial activity of essential oils was evaluated in terms of its minimum inhibitory concentration (MIC) values by the broth microdilution method in 96-well microplates. Both CL-EO and CP-EO displayed some activity against all oral pathogens under investigation; MIC values ranged from 20 to 200 µg/mL. CL-EO and CP-EO not only had promising activity against Streptococcus mutans (MIC = 20 µg/mL) and Lactobacillus casei (31.25 µg/mL), but also displayed antibacterial activity against all studied cariogenic bacteria. Efficacy of essential oils against S. mutans and L. casei is noteworthy and should be further investigated.

Resultados: N/A

Conclusão: N/A

Microencapsulation of lime (Citrus aurantifolia) oil for antibacterial finishing of cotton fabric.

Autores: Euis Julaeha, Sandra Puspita, Diana Rakhmawaty Eddy, Tatang Wahyudi, Mohamad Nurzaman, Jakariya Nugraha, Tati Herlina, Jamaludin Al Anshori | Ano: 2021 | Database: pubmed

Resumo: Functional cotton fabric incorporated with antibacterial microcapsules of lime (C. aurantifolia) essential oil (LO) was prepared. The coacervation method, employing two biopolymers of alginate and gelatin as the shells, was preferentially selected to produce the LO microcapsules, whereas immobilization of the LO microcapsules onto the fabric was done using the pad-dry-cure method using various concentrations of citric acid binder. The antibacterial inhibition zone of the functional fabric was subsequently analysed using the Kirby Bauer method. The LO microcapsules were produced with a yield, encapsulation efficiency (EE), and oil content (OC) of 47 ± 4%, 84 ± 11%, and 58 ± 4%, respectively. The homogenous spherical and soft microcapsules (1.554 μm) bonded effectively by 4% citric acid onto the surface of the fabric and detached back by only 3% after 15 cycles of washing. Overall, the optimized functional fabric exhibited the highest antibacterial activities among others against typical skin bacteria, such as S. aureus, E. coli, K. pneumoniae, and S. epidermidis, and thus it can be potentially applied to obtain antibacterial functional textile.

Resultados: N/A

Conclusão: N/A

Inhibition of cholinesterase by essential oil from food plant.

Autores: Wantida Chaiyana, Siriporn Okonogi | Ano: 2012 | Database: pubmed

Resumo: Inhibition of cholinesterase has attracted much attention recently because of its potential for the treatment of Alzheimer's disease. In this work, the anticholinesterase activities of plant oils were investigated using Ellman's colorimetric method. The results indicate that essential oils obtained from Melissa officinalis leaf and Citrus aurantifolia leaf showed high acetylcholinesterase and butyrylcholinesterase co-inhibitory activities. C. aurantifolia leaf oil revealed in this study has an IC(50) value on acetylcholinesterase and butyrylcholinesterase of 139 ± 35 and 42 ± 5 μg/ml, respectively. GC/MS analysis revealed that the major constituents of C. aurantifolia leaf oil are monoterpenoids including limonene, l-camphor, citronellol, o-cymene and 1,8-cineole.

Resultados: N/A

Conclusão: N/A

Inhibition of cholinesterase by essential oil from food plant.

Autores: Wantida Chaiyana, Siriporn Okonogi | Ano: 2012 | Database: pubmed

Resumo: Inhibition of cholinesterase has attracted much attention recently because of its potential for the treatment of Alzheimer's disease. In this work, the anticholinesterase activities of plant oils were investigated using Ellman's colorimetric method. The results indicate that essential oils obtained from Melissa officinalis leaf and Citrus aurantifolia leaf showed high acetylcholinesterase and butyrylcholinesterase co-inhibitory activities. C. aurantifolia leaf oil revealed in this study has an IC(50) value on acetylcholinesterase and butyrylcholinesterase of 139 ± 35 and 42 ± 5 μg/ml, respectively. GC/MS analysis revealed that the major constituents of C. aurantifolia leaf oil are monoterpenoids including limonene, l-camphor, citronellol, o-cymene and 1,8-cineole.

Resultados: N/A

Conclusão: N/A

Screening da Atividade Antifúngica de Óleos Essenciais sobre Cepas de Candida/ Screening of Essential Oils Antifungal Activity on Candida Strains

Autores: Castro, Ricardo Dias de; Lima, Edeltrudes de Oliveira | Ano: 2011 | Database: LILACS

Resumo: Objetivo: O propósito desse estudo foi identificar a atividade antifúngica de óleos essenciais sobre cepas de Candida envolvidas com infecções da cavidade bucal. Método: Foram avaliados óleos essenciais obtidos a partir das seguintes espécies vegetais: Citrus reticulata (Tangerina Cravo); Citrus aurantifolia (Limão Tahiti); Cinnamomum zeylanicum (Canela); Matricaria chamomilla (Camomila Azul); Mentha piperita (Menta); Eugenia uniflora (Pitanga) e Zingiber officinale (Gengibre). A determinação da atividade antifúngica foi realizada utilizando a técnica de difusão em meio de cultura sólido, onde discos de papel de filtro foram embebidos nos óleos e colocados em placas de Petri contendo agar Sabouraud Dextrose inoculado com cepas de Candida albicans e C. tropicalis. Também foi observada a concentração inibitória mínima a partir do método da microdiluição. Os ensaios foram realizados em duplicata. Resultados: Foi observada expressiva atividade antifúngica dos óleos essenciais de C. zeylanicum, C. aurantifolia e M. piperita, que apresentaram diâmetros de halos de inibição de crescimento microbiano de até, respectivamente, 48 mm, 30 mm e 19 mm. Ainda foi possível identificar que 66,7% das cepas ensaiadas mostraram-se resistentes aos óleos essenciais de C. reticulata, M. chamomilla, E. uniflora e Z. officinale. O C. zeylanicum e nistatina apresentaram, respectivamente, CIMs de 312 µg mL-1 e 32 µg mL-1. Conclusão: Os óleos essenciais avaliados apresentam atividade antifúngica, sendo os melhores resultados encontrados para C. zeylanicum. Sugere-se a realização de outros ensaios para avaliação de atividade anti-Candida desse óleo essencial, que pode representar possível agente terapêutico no tratamento de infecções fúngicas da cavidade bucal

Resultados: N/A

Conclusão: N/A

Atividade antifúngica de óleo essencial de limão (Citrus aurantifolia) frente a fungos filamentosos isolados de pães/ Antifungal activity of essential oil of lemon (Citrus aurantifolia) against filamentous fungi isolated from bread

Autores: Andrade, Francisca Joyce Elmiro Timbó; Torres, Nayannya Magalhães Monte Melo; Moraes, Georgia Maciel Dias de; Frutuoso, Antonia Elaine | Ano: 2015 | Database: LILACS

Resumo: N/A

Resultados: N/A

Conclusão: N/A

Using Citrus aurantifolia essential oil for the potential biocontrol of Colocasia esculenta (taro) leaf blight caused by Phytophthora colocasiae.

Autores: Séverin Nguemezi Tchameni, Staelle Njamou Mbiakeu, Modeste Lambert Sameza, Pierre Michel Dongmo Jazet, François Tchoumbougnang | Ano: 2018 | Database: pubmed

Resumo: The aim of this work was to evaluate the antimicrobial activities of leaves and epicarp of Citrus aurantifolia essential oil against Phytophthora colocasiae, the causative agent of taro leaf blight. Oils were extracted by hydrodistillation, and their chemical composition was determined by gas chromatography and gas chromatography coupled with mass spectrometry. Antimicrobial activities of oils were tested in vitro against mycelium growth and sporangium production. In situ tests were done on healthy taro leaves, and the necrosis symptoms were evaluated. Results showed that the essential oil extraction yields from leaves and epicarp were 0.61 and 0.36%, respectively. Limonene (48.96%), bornyl acetate (14.18%), geraniol (10.53%), geranial (3.93%), and myrcene (3.14%) were the main components in leaf oil, while limonene (59.09%), cis-hydrate sabinene (7.53%), geranial (5.61%), myrtenol (5.02%), and terpinen-4-ol (3.48%) were the main components in epicarp oil. Both oils exhibited antimicrobial activities with total inhibition of the mycelium growth at 500 and 900 ppm for leaf and epicarp, respectively. The highest inhibitory concentration of sporangium production was 400 (72.84%) and 800 ppm (80.65%) for leaf and epicarp oil, respectively. For the standard fungicide (metalaxyl), the total inhibition value of mycelial growth and sporangium production was 750 ppm. In situ tests showed that, at 5000 ppm, total inhibition (100%) was obtained for a preventive test, while 50% of the inhibition was observed for a curative test when leaf oil was applied. When epicarp essential oil was applied at 5000 ppm, 47.5 and 16.66% of the reduction of leaf necrosis were observed for the preventive and curative test, respectively. There were positive correlations between both the oil concentration and the reduction of necrosis caused by P. colocasiae. These findings suggest that the C. aurantifolia essential oil could serve as an eco-friendly biocontrol for the management of taro leaf blight.

Resultados: N/A

Conclusão: N/A

Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman.

Autores: Maha S Al-Aamri, Nour M Al-Abousi, Sausan S Al-Jabri, Tanveer Alam, Shah A Khan | Ano: 2018 | Database: pubmed

Resumo: This study investigated the chemical composition and the antioxidant and antimicrobial activities of the essential oil isolated from the aerial parts of Citrus aurantifolia L.

Resultados: Thirty-three chemical compounds were identified, with d-limonene (63.35%) forming the major constituent. Other prominent constituents include 3,7-dimethyl-2,6-octadien-1-ol (7.07%), geraniol (6.23%), E-citral (4.35%), Z-citral (3.29%), and β-ocimene (2.25%). The essential oil of Citrus aurantifolia L. leaves showed excellent antibacterial activity against Staphylococcus aureus and moderate activity against pathogenic Escherichia coli strains. The oil exhibited promising in-vitro antioxidant activity (IC50 value = 21.57 μg/mL) but showed moderate antibacterial activities.

Conclusão: N/A

Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus.

Autores: Luis Eduardo Ferreira, Bruno Iglesias Benincasa, Ana Lúcia Fachin, Silvia Helena Taleb Contini, Suzelei Castro França, Ana Carolina Souza Chagas, Rene Oliveira Beleboni | Ano: 2018 | Database: pubmed

Resumo: Infections of sheep with gastrointestinal parasites, especially Haemonchus contortus, have caused serious losses in livestock production, particularly after the emergence of resistance to conventional anthelmintics. The search for new anthelmintic agents, especially those of botanical origin, has grown substantially due to the perspective of less contamination of meat and milk, as well as other advantages related to their cost and accessibility in less developed countries. The aim of this study was to evaluate the in vitro anthelmintic activity of essential oils of the plant species Citrus aurantifolia, Anthemis nobile and Lavandula officinalis against the main developmental stages of the parasite H. contortus.

Resultados: Plant species were selected based on substantial ethnopharmacological information. Analysis of the composition of each oil by gas chromatography coupled to mass spectrometry (GC-MS) demonstrated the presence of limonene (56.37%), isobutyl angelate (29.26%) and linalool acetate (35.97%) as the major constituents in C. aurantifolia, A. nobile and L. officinalis, respectively. Different concentrations of each oil were tested in vitro for their capacity to inhibit egg hatching (EHT), larval development (LDT) and adult worm motility (AWMT) using a multidrug-resistant strain of H. contortus (Embrapa 2010). The IC50 values obtained for the oils of C. aurantifolia, A. nobile and L. officinalis were 0.694, 0.842 and 0.316 mg/ml in the EHT and 0.044, 0.117 and 0.280 mg/ml in the LDT, respectively. The three oils were able to inhibit adult worm motility completely within the first 8-12 h of observation in the AWMT.

Conclusão: N/A

Microencapsulation of lime (Citrus aurantifolia) oil for antibacterial finishing of cotton fabric.

Autores: Euis Julaeha, Sandra Puspita, Diana Rakhmawaty Eddy, Tatang Wahyudi, Mohamad Nurzaman, Jakariya Nugraha, Tati Herlina, Jamaludin Al Anshori | Ano: 2021 | Database: pubmed

Resumo: Functional cotton fabric incorporated with antibacterial microcapsules of lime (C. aurantifolia) essential oil (LO) was prepared. The coacervation method, employing two biopolymers of alginate and gelatin as the shells, was preferentially selected to produce the LO microcapsules, whereas immobilization of the LO microcapsules onto the fabric was done using the pad-dry-cure method using various concentrations of citric acid binder. The antibacterial inhibition zone of the functional fabric was subsequently analysed using the Kirby Bauer method. The LO microcapsules were produced with a yield, encapsulation efficiency (EE), and oil content (OC) of 47 ± 4%, 84 ± 11%, and 58 ± 4%, respectively. The homogenous spherical and soft microcapsules (1.554 μm) bonded effectively by 4% citric acid onto the surface of the fabric and detached back by only 3% after 15 cycles of washing. Overall, the optimized functional fabric exhibited the highest antibacterial activities among others against typical skin bacteria, such as S. aureus, E. coli, K. pneumoniae, and S. epidermidis, and thus it can be potentially applied to obtain antibacterial functional textile.

Resultados: N/A

Conclusão: N/A

Effects of essential oils on native and recombinant acetylcholinesterases of Rhipicephalus microplus.

Autores: Everton Gomes Guimarães Dos Santos, Wallyson André Dos Santos Bezerra, Kevin B Temeyer, Adalberto A Pérez de León, Livio Martins Costa-Junior, Alexandra Martins Dos Santos Soares | Ano: 2021 | Database: pubmed

Resumo: This study reports the action of essential oils (EO) from five plants on the activity of native and recombinant acetylcholinesterases (AChE) from Rhipicephalus microplus. Enzyme activity of native susceptible AChE extract (S.AChE), native resistant AChE extract (R.AChE), and recombinant enzyme (rBmAChE1) was determined. An acetylcholinesterase inhibition test was used to verify the effect of the EO on enzyme activity. EO from Eucalyptus globulus, Citrus aurantifolia, Citrus aurantium var.dulcis inhibited the activity of S.AChE and R.AChE. Oils from the two Citrus species inhibited S.AChE and R.AChE in a similar way while showing greater inhibition on R.AChE. The oil from E. globulus inhibited native AChE, but no difference was observed between the S.AChE and R.AChE; however, 71% inhibition for the rBmAChE1 was recorded. Mentha piperita oil also inhibited S.AChE and R.AChE, but there was significant inhibition at the highest concentration tested. Cymbopogon winterianus oil did not inhibit AChE. Further studies are warranted with the oils from the two Citrus species that inhibited R.AChE because of the problem with R. microplus resistant to organophosphates, which target AChE. C. winterianus oil can be used against R. microplus populations that are resistant to organophosphates because its acaricidal properties act by mechanism(s) other than AChE inhibition.

Resultados: N/A

Conclusão: N/A

Comparative study on the antioxidant capacity and cholinesterase inhibitory activity of Citrus aurantifolia Swingle, C. aurantium L., and C. bergamia Risso and Poit. peel essential oils.

Autores: Rosa Tundis, Monica Rosa Loizzo, Marco Bonesi, Federica Menichini, Vincenzo Mastellone, Carmela Colica, Francesco Menichini | Ano: 2012 | Database: pubmed

Resumo: The interest in medicinal plant research and in the aroma-therapeutic effects of essential oils in humans has increased in recent years, especially for the treatment of pathologies of relevant social impact such as Alzheimer's disease. The present study was taken up to evaluate the antioxidant capacity and the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the peel essential oils from three Citrus species, C. aurantifolia Swingle, C. aurantium L., and C. bergamia Risso & Poit. Essential oils were analyzed by GC and GC-MS and they contain mainly limonene, α-pinene, β-pinene, γ-terpinene, and linalyl acetate. C. aurantifolia oil showed the highest radical scavenging activity on ABTS assay (IC₅₀ value of 19.6 μg/mL), while C. bergamia exhibited a good antioxidant activity evaluated by the β-carotene bleaching test (IC₅₀ = 42.6 μg/mL after 60 min of incubation). C. aurantifolia inhibited more selectively AChE. Obtained data suggest a potential use of Citrus oils as a valuable new flavor with functional properties for food or nutraceutical products with particular relevance to supplements for the elderly.

Resultados: N/A

Conclusão: N/A

Comparative study on the antioxidant capacity and cholinesterase inhibitory activity of Citrus aurantifolia Swingle, C. aurantium L., and C. bergamia Risso and Poit. peel essential oils.

Autores: Rosa Tundis, Monica Rosa Loizzo, Marco Bonesi, Federica Menichini, Vincenzo Mastellone, Carmela Colica, Francesco Menichini | Ano: 2012 | Database: pubmed

Resumo: The interest in medicinal plant research and in the aroma-therapeutic effects of essential oils in humans has increased in recent years, especially for the treatment of pathologies of relevant social impact such as Alzheimer's disease. The present study was taken up to evaluate the antioxidant capacity and the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the peel essential oils from three Citrus species, C. aurantifolia Swingle, C. aurantium L., and C. bergamia Risso & Poit. Essential oils were analyzed by GC and GC-MS and they contain mainly limonene, α-pinene, β-pinene, γ-terpinene, and linalyl acetate. C. aurantifolia oil showed the highest radical scavenging activity on ABTS assay (IC₅₀ value of 19.6 μg/mL), while C. bergamia exhibited a good antioxidant activity evaluated by the β-carotene bleaching test (IC₅₀ = 42.6 μg/mL after 60 min of incubation). C. aurantifolia inhibited more selectively AChE. Obtained data suggest a potential use of Citrus oils as a valuable new flavor with functional properties for food or nutraceutical products with particular relevance to supplements for the elderly.

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Conclusão: N/A

Lime (Citrus aurantifolia (Christm.) Swingle) Essential Oils: Volatile Compounds, Antioxidant Capacity, and Hypolipidemic Effect.

Autores: Li-Yun Lin, Cheng-Hung Chuang, Hsin-Chun Chen, Kai-Min Yang | Ano: 2019 | Database: pubmed

Resumo: Lime peels are mainly obtained from the byproducts of the juice manufacturing industry, which we obtained and used to extract essential oil (2.3%) in order to examine the antioxidant and hypolipidaemic effects. We identified 60 volatile compounds of lime essential oil (LEO) with GC/MS, of which the predominant constituents were limonene, γ-terpinene, and β-pinene. Lime essential oil was measured according to the DPPH assay and ABTS assay, with IC50 values of 2.36 mg/mL and 0.26 mg/mL, respectively. This study also explored the protective effects of LEO against lipid-induced hyperlipidemia in a rat model. Two groups of rats received oral LEO in doses of 0.74 g/100 g and 2.23 g/100 g with their diets. Eight weeks later, we found that the administration of LEO improved the serum total cholesterol, triglyceride, low-density lipoprotein cholesterol, alanine aminotransferase, and aspartate transaminase levels in the hyperlipidemic rats (p < 0.05). Simultaneously, the LEO improved the health of the rats in terms of obesity, atherogenic index, and fatty liver.

Resultados: N/A

Conclusão: N/A

Lime (Citrus aurantifolia (Christm.) Swingle) Essential Oils: Volatile Compounds, Antioxidant Capacity, and Hypolipidemic Effect.

Autores: Li-Yun Lin, Cheng-Hung Chuang, Hsin-Chun Chen, Kai-Min Yang | Ano: 2019 | Database: pubmed

Resumo: Lime peels are mainly obtained from the byproducts of the juice manufacturing industry, which we obtained and used to extract essential oil (2.3%) in order to examine the antioxidant and hypolipidaemic effects. We identified 60 volatile compounds of lime essential oil (LEO) with GC/MS, of which the predominant constituents were limonene, γ-terpinene, and β-pinene. Lime essential oil was measured according to the DPPH assay and ABTS assay, with IC50 values of 2.36 mg/mL and 0.26 mg/mL, respectively. This study also explored the protective effects of LEO against lipid-induced hyperlipidemia in a rat model. Two groups of rats received oral LEO in doses of 0.74 g/100 g and 2.23 g/100 g with their diets. Eight weeks later, we found that the administration of LEO improved the serum total cholesterol, triglyceride, low-density lipoprotein cholesterol, alanine aminotransferase, and aspartate transaminase levels in the hyperlipidemic rats (p < 0.05). Simultaneously, the LEO improved the health of the rats in terms of obesity, atherogenic index, and fatty liver.

Resultados: N/A

Conclusão: N/A

Using Citrus aurantifolia essential oil for the potential biocontrol of Colocasia esculenta (taro) leaf blight caused by Phytophthora colocasiae.

Autores: Séverin Nguemezi Tchameni, Staelle Njamou Mbiakeu, Modeste Lambert Sameza, Pierre Michel Dongmo Jazet, François Tchoumbougnang | Ano: 2018 | Database: pubmed

Resumo: The aim of this work was to evaluate the antimicrobial activities of leaves and epicarp of Citrus aurantifolia essential oil against Phytophthora colocasiae, the causative agent of taro leaf blight. Oils were extracted by hydrodistillation, and their chemical composition was determined by gas chromatography and gas chromatography coupled with mass spectrometry. Antimicrobial activities of oils were tested in vitro against mycelium growth and sporangium production. In situ tests were done on healthy taro leaves, and the necrosis symptoms were evaluated. Results showed that the essential oil extraction yields from leaves and epicarp were 0.61 and 0.36%, respectively. Limonene (48.96%), bornyl acetate (14.18%), geraniol (10.53%), geranial (3.93%), and myrcene (3.14%) were the main components in leaf oil, while limonene (59.09%), cis-hydrate sabinene (7.53%), geranial (5.61%), myrtenol (5.02%), and terpinen-4-ol (3.48%) were the main components in epicarp oil. Both oils exhibited antimicrobial activities with total inhibition of the mycelium growth at 500 and 900 ppm for leaf and epicarp, respectively. The highest inhibitory concentration of sporangium production was 400 (72.84%) and 800 ppm (80.65%) for leaf and epicarp oil, respectively. For the standard fungicide (metalaxyl), the total inhibition value of mycelial growth and sporangium production was 750 ppm. In situ tests showed that, at 5000 ppm, total inhibition (100%) was obtained for a preventive test, while 50% of the inhibition was observed for a curative test when leaf oil was applied. When epicarp essential oil was applied at 5000 ppm, 47.5 and 16.66% of the reduction of leaf necrosis were observed for the preventive and curative test, respectively. There were positive correlations between both the oil concentration and the reduction of necrosis caused by P. colocasiae. These findings suggest that the C. aurantifolia essential oil could serve as an eco-friendly biocontrol for the management of taro leaf blight.

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Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species.

Autores: Jorge Luis Amorim, Daniel Luiz Reis Simas, Mariana Martins Gomes Pinheiro, Daniela Sales Alviano Moreno, Celuta Sales Alviano, Antonio Jorge Ribeiro da Silva, Patricia Dias Fernandes | Ano: 2016 | Database: pubmed

Resumo: Citrus fruits have potential health-promoting properties and their essential oils have long been used in several applications. Due to biological effects described to some citrus species in this study our objectives were to analyze and compare the phytochemical composition and evaluate the anti-inflammatory effect of essential oils (EO) obtained from four different Citrus species. Mice were treated with EO obtained from C. limon, C. latifolia, C. aurantifolia or C. limonia (10 to 100 mg/kg, p.o.) and their anti-inflammatory effects were evaluated in chemical induced inflammation (formalin-induced licking response) and carrageenan-induced inflammation in the subcutaneous air pouch model. A possible antinociceptive effect was evaluated in the hot plate model. Phytochemical analyses indicated the presence of geranial, limonene, γ-terpinene and others. EOs from C. limon, C. aurantifolia and C. limonia exhibited anti-inflammatory effects by reducing cell migration, cytokine production and protein extravasation induced by carrageenan. These effects were also obtained with similar amounts of pure limonene. It was also observed that C. aurantifolia induced myelotoxicity in mice. Anti-inflammatory effect of C. limon and C. limonia is probably due to their large quantities of limonene, while the myelotoxicity observed with C. aurantifolia is most likely due to the high concentration of citral. Our results indicate that these EOs from C. limon, C. aurantifolia and C. limonia have a significant anti-inflammatory effect; however, care should be taken with C. aurantifolia.

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Microencapsulation of Citrus aurantifolia essential oil with the optimized CaCl2 crosslinker and its antibacterial study for cosmetic textiles.

Autores: Luthfia Pratiwi, Diana Rakhmawaty Eddy, Jamaludin Al Anshori, Asep Harja, Tatang Wahyudi, Agus Surya Mulyawan, Euis Julaeha | Ano: 2022 | Database: pubmed

Resumo: A functional fabric immobilized by the microcapsules of C. aurantifolia lime essential oil (LO) was prepared and characterized. A varied amount of CaCl2 crosslinker was optimized to coacervate LO using alginate-gelatin biopolymers and Tween 80 emulsifier. A further evaluation of the immobilized LO microcapsules for the antibacterial effect against both Gram-positive and Gram-negative bacteria was conducted. The optimized alginate/gelatin-based microcapsules were effectively crosslinked by 15% CaCl2 with an yield, oil content (OC), and encapsulation efficiency (EE) of 39.91 ± 3.10%, 78.33 ± 7.53%, and 90.27 ± 5.84%, respectively. A spherical shape of LO microcapsules was homogeneously found with an average particle size of 1.394 μm. A first-order kinetics mechanism for the release of LO out of the microcapsules was modeled by Avrami's kinetic equation (k = 1.60 ± 3.68 10-5 s-1). The LO microcapsules demonstrated good thermal stability up to 100 °C and maintained 51.07% OC and 43.56% EE at ambient temperature for three weeks. Using a pad dry method and citric acid binder, LO microcapsules were successfully immobilized on a cloth with a % add on 30.60 ± 1.80%. The LO microcapsules and the immobilized one exhibited a moderate ZoI of bacterial growth for Gram-positive S. aureus and S. epidermidis as well as Gram-negative E. coli and K. pneumonia. Further washing test toward the functional fabric showed that the LO microcapsules incorporated into the fabric were resistant to five cycles of normal washing with a mass reduction of 22.01 ± 1.69%.

Resultados: N/A

Conclusão: N/A

Microencapsulation of Citrus aurantifolia essential oil with the optimized CaCl2 crosslinker and its antibacterial study for cosmetic textiles.

Autores: Luthfia Pratiwi, Diana Rakhmawaty Eddy, Jamaludin Al Anshori, Asep Harja, Tatang Wahyudi, Agus Surya Mulyawan, Euis Julaeha | Ano: 2022 | Database: pubmed

Resumo: A functional fabric immobilized by the microcapsules of C. aurantifolia lime essential oil (LO) was prepared and characterized. A varied amount of CaCl2 crosslinker was optimized to coacervate LO using alginate-gelatin biopolymers and Tween 80 emulsifier. A further evaluation of the immobilized LO microcapsules for the antibacterial effect against both Gram-positive and Gram-negative bacteria was conducted. The optimized alginate/gelatin-based microcapsules were effectively crosslinked by 15% CaCl2 with an yield, oil content (OC), and encapsulation efficiency (EE) of 39.91 ± 3.10%, 78.33 ± 7.53%, and 90.27 ± 5.84%, respectively. A spherical shape of LO microcapsules was homogeneously found with an average particle size of 1.394 μm. A first-order kinetics mechanism for the release of LO out of the microcapsules was modeled by Avrami's kinetic equation (k = 1.60 ± 3.68 10-5 s-1). The LO microcapsules demonstrated good thermal stability up to 100 °C and maintained 51.07% OC and 43.56% EE at ambient temperature for three weeks. Using a pad dry method and citric acid binder, LO microcapsules were successfully immobilized on a cloth with a % add on 30.60 ± 1.80%. The LO microcapsules and the immobilized one exhibited a moderate ZoI of bacterial growth for Gram-positive S. aureus and S. epidermidis as well as Gram-negative E. coli and K. pneumonia. Further washing test toward the functional fabric showed that the LO microcapsules incorporated into the fabric were resistant to five cycles of normal washing with a mass reduction of 22.01 ± 1.69%.

Resultados: N/A

Conclusão: N/A

Volatile composition and biological activity of key lime Citrus aurantifolia essential oil.

Autores: Federica Spadaro, Rosaria Costa, Clara Circosta, Francesco Occhiuto | Ano: 2012 | Database: pubmed

Resumo: The essential oil of Citrus aurantifolia (Christm) Swingle fruits (limes) was studied for its potential spasmolytic effects in relation to its chemical composition. The essential oil, extracted by hydrodistillation (HD), was analyzed by GC-FID and GC-MS. The antispasmodic activity was evaluated on isolated rabbit jejunum, aorta and uterus. The results indicated that the essential oil of C. aurantifolia possesses important spasmolytic properties, which are likely to be due to its major constituents, limonene (58.4%), beta-pinene (15.4%), gamma-terpinene (8.5%), and citral (4.4%).

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Conclusão: N/A

Fumigant activity of essential oils from Cinnamomum and Citrus spp. and pure compounds against Dermanyssus gallinae (De Geer) (Acari: Dermanyssidae) and toxicity toward the nontarget organism Beauveria bassiana (Vuill.).

Autores: Cristina Bordin, Dejane Santos Alves, Luis Francisco Angeli Alves, Murilo Silva de Oliveira, Jociani Ascari, Dilamara Riva Scharf | Ano: 2021 | Database: pubmed

Resumo: Dermanyssus gallinae(De Geer) (Acari: Dermanyssidae) is the main ectoparasite associated with laying poultry. This mite is commonly controlled by the application of synthetic chemical insecticides, wich lead to the selection of resistant populations and formation of residues in eggs. Thus, new molecules must be developed to control D. gallinae. This work evaluated the toxicity of essential oils (EOs) from Cinnamomum cassia, Cinnamomum camphora, Cinnamomum camphora var. linalooliferum, Citrus aurantium, Citrus aurantium var. bergamia, Citrus aurantifolia and Citrus reticulata var. tangerine against D. gallinae. Additionally, the chemical profiles of the most bioactive EOs were analyzed by gas chromatography coupled with mass spectrometry (GC-MS) and the major compounds were subjected to new tests using D. gallinae. The most toxic EOs against D. gallinae were evaluated for the nontarget entomopathogenic fungus Beauveria bassiana (Unioeste 88). The EOs from C. cassia (LC50 = 25.43 ± 1.0423 μg/cm3) and C. camphora var. linalooliferum (LC50 = 39.84 ± 1.9635 μg/cm3) were the most active in the fumigant bioassay and caused mortality rates of 96 and 61%, respectively. The GC-MS analysis revealed that the major constituents of EOs from C. cassia and C. camphora var. linalooliferum were trans-cinnamaldehyde and linalool, respectively. The pure compounds, trans-cinnamaldehyde (LC50 = 68.89 ± 3.1391 μg/cm3) and linalool (LC50 = 51.45 ± 1.1967 μg/cm3), were tested on D. gallinae and showed lower toxicity than the EOs. Thus, the compounds were not the only active substances produced by C. cassia and C. camphora var. linalooliferum; moreover synergism may have occurred between the substances. The EOs from C. cassia and C. camphora var. linalooliferum were also toxic to B. bassiana (Unioeste 88). Thus, EOs from C. cassia and C. camphora var. linalooliferum are promising candidates for use in D. gallinae control, but cannot be used in conjunction with the fungus B. bassiana.

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Hexosomal Dispersion: A Nano-Based Approach to Boost the Antifungal Potential of Citrus Essential Oils against Plant Fungal Pathogens.

Autores: Mohamed S Sedeek, Abdulaziz M Al-Mahallawi, Rania A A Hussien, Ahmed M Abdelhaleem Ali, Ibrahim A Naguib, Mai K Mansour | Ano: 2021 | Database: pubmed

Resumo: The demand for natural fungicides to replace synthetic ones has surged since toxic residues persist in soils, causing environmental contamination and posing a serious threat to worldwide public health. In the context of crop protection and enhancing the efficiency and safety of fungicides, nanotechnology is an eco-friendly strategy in managing fungal pathogens. In the present study, essential oils were isolated from the peels of four citrus fruits (Citrus lemon, Citrus aurantifolia, Citrus maxima, and Citrus sinensis) and were investigated using gas chromatography-mass spectrometric analysis. Monoterpene hydrocarbon was the most predominant group and limonene was the most abundant in the four oils. The antifungal potential of the oils was investigated, and the most active oil (Citrus lemon) was loaded into hexosomal dispersion, and its antifungal potential was retested against the same fungi. The structurally unique nano-based formulation showed great potency for fungal control. To the best of our knowledge, it is the first time the oil of Citrus lemon in nano-hexosomes has been formulated and its fungicidal activity examined. The data collected suggest that citrus essential oils (CEOs), especially when nano-formulated, could be successfully used in integrated fungus management programs.

Resultados: N/A

Conclusão: N/A

Volatile composition and biological activity of key lime Citrus aurantifolia essential oil.

Autores: Federica Spadaro, Rosaria Costa, Clara Circosta, Francesco Occhiuto | Ano: 2012 | Database: pubmed

Resumo: The essential oil of Citrus aurantifolia (Christm) Swingle fruits (limes) was studied for its potential spasmolytic effects in relation to its chemical composition. The essential oil, extracted by hydrodistillation (HD), was analyzed by GC-FID and GC-MS. The antispasmodic activity was evaluated on isolated rabbit jejunum, aorta and uterus. The results indicated that the essential oil of C. aurantifolia possesses important spasmolytic properties, which are likely to be due to its major constituents, limonene (58.4%), beta-pinene (15.4%), gamma-terpinene (8.5%), and citral (4.4%).

Resultados: N/A

Conclusão: N/A

Cold-pressed oil from Citrus aurantifolia inhibits the proliferation of vascular smooth muscle cells via regulation of PI3K/MAPK signaling pathways.

Autores: Byeong-Wook Song, Chang Youn Lee, Jun-Hee Park, Bomi Kim, Seahyoung Lee, Soyeon Lim, Sang Woo Kim, Jung-Won Choi, Misun Kang, Jung Hwa Kang, Sung-Suk Lee, Mi-Jin Park, Hanbyeol Moon, Ki-Chul Hwang, Il-Kwon Kim | Ano: 2022 | Database: pubmed

Resumo: Vascular occlusive disease is a chronic disease with significant morbidity and mortality. Although a variety of therapies and medications have been developed, the likelihood of disease re-emergence is high and this can be life-threatening. Based on a previous screening experiment related to vascular obstructive diseases using 34 types of essential oils, cold-pressed oil (CpO) from Citrus aurantifolia (lime) has been demonstrated to have the best effect for the inhibition of vascular smooth muscle cells (VSMCs) proliferation. The aim of the present study was to evaluate the effect of lime CpO on the pathological changes of VSMCs. To determine this, the effect of lime CpO on VSMC proliferation, a major cause of vascular disease, was investigated. To determine the safe concentration interval for toxicity of CpO during VSMC culture, a dilution of 1x10-5 was determined using Cell Counting Kit-8 assay, which was confirmed to be non-toxic using a lactate dehydrogenase assay. To examine the effect of lime CpO in cellular signaling pathways, changes in phosphorylation of both the PI3K/AKT/mTOR and extracellular signal-regulated MEK/ERK signaling pathways with serum were investigated. Furthermore, lime CpO with FBS also significantly decreased the expression levels of the cell cycle regulators cyclin D1 and proliferating cell nuclear antigen. Additionally, lime CpO with FBS significantly inhibited the sprouting of VSMCs in an ex vivo culture system. These results suggested that lime CpO inhibited the abnormal proliferation of VSMCs and can be developed as a nature-based therapeutic agent for obstructive vascular disease.

Resultados: N/A

Conclusão: N/A

Cold-pressed oil from Citrus aurantifolia inhibits the proliferation of vascular smooth muscle cells via regulation of PI3K/MAPK signaling pathways.

Autores: Byeong-Wook Song, Chang Youn Lee, Jun-Hee Park, Bomi Kim, Seahyoung Lee, Soyeon Lim, Sang Woo Kim, Jung-Won Choi, Misun Kang, Jung Hwa Kang, Sung-Suk Lee, Mi-Jin Park, Hanbyeol Moon, Ki-Chul Hwang, Il-Kwon Kim | Ano: 2022 | Database: pubmed

Resumo: Vascular occlusive disease is a chronic disease with significant morbidity and mortality. Although a variety of therapies and medications have been developed, the likelihood of disease re-emergence is high and this can be life-threatening. Based on a previous screening experiment related to vascular obstructive diseases using 34 types of essential oils, cold-pressed oil (CpO) from Citrus aurantifolia (lime) has been demonstrated to have the best effect for the inhibition of vascular smooth muscle cells (VSMCs) proliferation. The aim of the present study was to evaluate the effect of lime CpO on the pathological changes of VSMCs. To determine this, the effect of lime CpO on VSMC proliferation, a major cause of vascular disease, was investigated. To determine the safe concentration interval for toxicity of CpO during VSMC culture, a dilution of 1x10-5 was determined using Cell Counting Kit-8 assay, which was confirmed to be non-toxic using a lactate dehydrogenase assay. To examine the effect of lime CpO in cellular signaling pathways, changes in phosphorylation of both the PI3K/AKT/mTOR and extracellular signal-regulated MEK/ERK signaling pathways with serum were investigated. Furthermore, lime CpO with FBS also significantly decreased the expression levels of the cell cycle regulators cyclin D1 and proliferating cell nuclear antigen. Additionally, lime CpO with FBS significantly inhibited the sprouting of VSMCs in an ex vivo culture system. These results suggested that lime CpO inhibited the abnormal proliferation of VSMCs and can be developed as a nature-based therapeutic agent for obstructive vascular disease.

Resultados: N/A

Conclusão: N/A

Growth Inhibition of Sulfate-Reducing Bacteria in Produced Water from the Petroleum Industry Using Essential Oils.

Autores: Pamella Macedo de Souza, Fátima Regina de Vasconcelos Goulart, Joana Montezano Marques, Humberto Ribeiro Bizzo, Arie Fitzgerald Blank, Claudia Groposo, Maíra Paula de Sousa, Vanessa Vólaro, Celuta Sales Alviano, Daniela Sales Alviano Moreno, Lucy Seldin | Ano: 2017 | Database: pubmed

Resumo: Strategies for the control of sulfate-reducing bacteria (SRB) in the oil industry involve the use of high concentrations of biocides, but these may induce bacterial resistance and/or be harmful to public health and the environment. Essential oils (EO) produced by plants inhibit the growth of different microorganisms and are a possible alternative for controlling SRB. We aimed to characterize the bacterial community of produced water obtained from a Brazilian petroleum facility using molecular methods, as well as to evaluate the antimicrobial activity of EO from different plants and their major components against Desulfovibrio alaskensis NCIMB 13491 and against SRB growth directly in the produced water. Denaturing gradient gel electrophoresis revealed the presence of the genera Pelobacter and Marinobacterium, Geotoga petraea, and the SRB Desulfoplanes formicivorans in our produced water samples. Sequencing of dsrA insert-containing clones confirmed the presence of sequences related to D. formicivorans. EO obtained from Citrus aurantifolia, Lippia alba LA44 and Cymbopogon citratus, as well as citral, linalool, eugenol and geraniol, greatly inhibited (minimum inhibitory concentration (MIC) = 78 µg/mL) the growth of D. alaskensis in a liquid medium. The same MIC was obtained directly in the produced water with EO from L. alba LA44 (containing 82% citral) and with pure citral. These findings may help to control detrimental bacteria in the oil industry.

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Conclusão: N/A

Insecticidal activities of Citrus aurantifolia essential oil against Aedes aegypti (Diptera: Culicidae).

Autores: Riju Sarma, Kamal Adhikari, Sudarshana Mahanta, Bulbuli Khanikor | Ano: 2019 | Database: pubmed

Resumo: In the recent time, global attention for the control of vectors has shifted from chemical insecticides to botanicals. In the present investigation, authors attempted to evaluate the efficacy of peel and leaf essential oil (EO) of Citrus aurantifolia against Aedes aegypti.

Resultados: The results revealed that both the oils possess more ovicidal activity (LC50 value of 5.26 ppm and 17.71 ppm for leaf and peel oil respectively at 72 h) than larvicidal activity. As larvicide, the essential oil from the peel of Citrus aurantifolia showed rapid effect with LC50 value of 128.81 ppm at 24 h which reduced to 106.77 ppm at 72 h while the leaf oil showed slow effect with LC50 value of 188.59 ppm, 107.37 ppm and 104.59 ppm at 24 h, 48 h and 72 h respectively. Again, the two essential oils did not show significant adulticidal activity. GC-MS analysis of both the oils recorded presence of different compounds. As a major constituent compound of the leaf EO of Citrus aurantifolia, citral was tested for their ovicidal, larvicidal and adulticidal activities against Aedes aegypti. The result showed highest ovicidal activities (LC50 value of 4.84 ppm at 72 h) of citral followed by larvicidal (LC50 value of 87.02 ppm at 24 h) and adulticidal (LC50 value of 103.88 ppm at 24 h) activities.

Conclusão: From this study, it can be concluded that the essential oil extracted from the leaf and peel of Citrus aurantifolia and one of its major constituent compound citral can be included in the mosquito control programme of Aedes aegypti.

Insecticidal properties of volatile extracts of orange peels.

Autores: F C Ezeonu, G I Chidume, S C Udedi | Ano: 2001 | Database: pubmed

Resumo: Statistical studies using the randomised complete block design with four replicates showed that volatile extracts of two species of orange peel--Citrus sinensis (sweet orange) and Citrus aurantifolia (lime) had insecticidal activity against mosquito, cockroach and housefly. Insecticidal activity was better after 60 min than at 30 min spraying of rooms. Volatile extracts of C. sinensis showed greater insecticidal potency, while the cockroach was the most susceptible to the orange peels among the three insects studied.

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Conclusão: N/A

Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.

Autores: Fatima Alaba Ibrahim, Lamidi Ajao Usman, Jubril Olayinka Akolade, Oluwafemi Ayodeji Idowu, Azeemat Titilola Abdulazeez, Aliyu Olalekan Amuzat | Ano: 2019 | Database: pubmed

Resumo: Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison. Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil. Other notable compounds identified were neral (7.81%), linalool (4.75%), sulcatone (3.48%) and isogeraniol (3.48%). Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.) to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased. Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks. Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug. Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype. The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.

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Conclusão: N/A

Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.

Autores: Fatima Alaba Ibrahim, Lamidi Ajao Usman, Jubril Olayinka Akolade, Oluwafemi Ayodeji Idowu, Azeemat Titilola Abdulazeez, Aliyu Olalekan Amuzat | Ano: 2019 | Database: pubmed

Resumo: Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison. Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil. Other notable compounds identified were neral (7.81%), linalool (4.75%), sulcatone (3.48%) and isogeraniol (3.48%). Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.) to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased. Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks. Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug. Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype. The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.

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Essential oil from Citrus aurantifolia prevents ketotifen-induced weight-gain in mice.

Autores: Solmaz Asnaashari, Abbas Delazar, Bohlol Habibi, Roghayeh Vasfi, Lutfun Nahar, Sanaz Hamedeyazdan, Satyajit D Sarker | Ano: 2010 | Database: pubmed

Resumo: Obesity is a major health problem world-wide. Medical intervention is often needed to tackle this problem, and accordingly the need for developing more effective, safer and cheaper weight reducing drugs has become paramount in recent years. In the present study, the effects of lime (Citrus aurantifolia) essential oils in reducing body weight, individually and in co-administration with ketotifen, an antihistaminic drug that causes weight gain, has been investigated using a mouse model. During the 45 days experimental period, the mice that received ketotifen demonstrated an enhancement both in the amount of food intake and body weight compared with the control group. Groups treated with lime essential oil displayed a reduction in body weight and food consumption in mice, possibly through promoting anorexia which might have played a role in weight loss. Interestingly, co-administration of the lime essential oil and ketotifen caused significant suppression in gaining weight, as well as decreased body weights of mice. The data obtained in this study suggested that lime essential oil plays an important role in weight loss and could be useful in the treatment of drug-induced obesity and related diseases. The GC-MS analysis of the essential oils of C. aurantifolia was also performed and approximately 22 main components, with limonene (28.27%) being the principal one, were identified and quantified.

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Conclusão: N/A

Evaluation of botanicals as repellents against mosquitoes.

Autores: N G Das, I Baruah, P K Talukdar, S C Das | Ano: 2003 | Database: pubmed

Resumo: Repellent properties of three plant extracts--essential oil (steam distillate) of Zanthoxylum limonella (fruits), Citrus aurantifolia (leaf) and petroleum ether extract of Z. limonella (fruits) were evaluated as repellent against Aedes (S.) albopictus mosquitoes in mustard (Dhara) and coconut (Parachute) oil base under laboratory conditions. Three concentrations--10, 20 and 30% of the repellents were evaluated. Repellents in mustard oil afforded longer protection time against the bites of Aedes (S.) albopictus mosquitoes than those in coconut oil. At 30% concentration, 296-304 min protection time was achieved by the test repellents in mustard oil base while repellents in coconut oil exhibited 223.5-245 min protection time at the same concentration. Oil of Z. limonella gave the highest protection time against the bites of Aedes (S.) albopictus mosquitoes at all the concentrations than other herbal repellents tested both in mustard and coconut oil.

Resultados: N/A

Conclusão: N/A

New Candidates for Plant-Based Repellents Against Aedes aegypti.

Autores: Norashiqin Misni, Zurainee Mohamed Nor, Rohani Ahmad | Ano: 2016 | Database: pubmed

Resumo: Based on an ethnobotanical study on use for plant species against mosquito bites in the Kota Tinggi District, Johor State, Malaysia, 3 plants selected for study, Citrus aurantifolia (leaves), Citrus grandis (fruit peel), and Alpinia galanga (rhizome), were extracted using hydrodistillation to produce essential oils. These essential oils were then formulated as a lotion using a microencapsulation process and then tested for their repellent effect against Aedes aegypti. N,N-diethyl-m-toluamide (deet) was also prepared in the same formulation and tested for repellency as controls. Four commercial plant-based repellent (KAPS(®), MozAway(®), BioZ Natural(®), and Mosiquard(®)) also were incorporated in the bioassay for comparison purposes. Bioassays revealed that at 20% concentration all repellent formulations demonstrated complete protection for 2 h and >90% for 4 h post-application. The A. galanga-based formulation provided the greatest level of protection (98.91%), which extended for 4 h post-application and was not significantly different from deet at similar concentration. When compared with commercial plant-based repellents (KAPS(®), MozAway(®), and BioZ Natural(®)), the 3 lotion formulations showed significantly better protection against Ae. aegypti bites, providing >90% protection for 4 h. In conclusion, our 3 plant-based lotion formulations provided acceptable levels of protection against host-seeking Ae. aegypti and should be developed.

Resultados: N/A

Conclusão: N/A

Repellent activity of some essential oils against Simulium species in India.

Autores: S Hazarika, Sunil Dhiman, Bipul Rabha, R K Bhola, Lokendera Singh | Ano: 2012 | Database: pubmed

Resumo: Use of repellents seems to be most reliable method of personal protection against annoyance and infections associated with haematophagous insects. We have investigated the biting activity of Simulium and tested the repellency of five essential oils extracted from Homalomena aromatica Schott (Alismatales: Araceae), Pogostemon heyneanus Bentham (Lamiales: Lamiaceae), Citrus aurantifolia Swingle (Sapindales: Rutaceae), Vitex negundo L. (Lamiales: Lamiaceae), and Ageratum conzoides L. (Asterales: Asteraceae) on the human volunteers against Simulium (blackflies) in three locations of Arunachal Pradesh, India. Blackflies preferred biting legs (> 79%) as compared to hand and face with profound biting activity during 1000-1100 h (> 23%) and 1500 - 1600 h (> 28%). The essential oil extracted from Homalomena aromatica, Vitex negundo and Ageratum conizoides provided > 2 h protection at 5% concentration and > 5 h protection at 10% concentration in all the three testing locations. The repellency of Homalomena aromatica, Vitex negundo and Ageratum conizoides essential oils after 6 h application was > 50% at 5% concentration and > 90% at 10% concentration. The study provides evidence for the potential of these essential oils in developing new repellents against blackflies.

Resultados: N/A

Conclusão: N/A

Evaluation of botanicals as repellents against mosquitoes.

Autores: N G Das, I Baruah, P K Talukdar, S C Das | Ano: 2003 | Database: pubmed

Resumo: Repellent properties of three plant extracts--essential oil (steam distillate) of Zanthoxylum limonella (fruits), Citrus aurantifolia (leaf) and petroleum ether extract of Z. limonella (fruits) were evaluated as repellent against Aedes (S.) albopictus mosquitoes in mustard (Dhara) and coconut (Parachute) oil base under laboratory conditions. Three concentrations--10, 20 and 30% of the repellents were evaluated. Repellents in mustard oil afforded longer protection time against the bites of Aedes (S.) albopictus mosquitoes than those in coconut oil. At 30% concentration, 296-304 min protection time was achieved by the test repellents in mustard oil base while repellents in coconut oil exhibited 223.5-245 min protection time at the same concentration. Oil of Z. limonella gave the highest protection time against the bites of Aedes (S.) albopictus mosquitoes at all the concentrations than other herbal repellents tested both in mustard and coconut oil.

Resultados: N/A

Conclusão: N/A

Chemical profile and in vivo toxicity evaluation of unripe Citrus aurantifolia essential oil.

Autores: Christian K Adokoh, Du-Bois Asante, Desmond O Acheampong, Yohei Kotsuchibashi, Francis A Armah, Ignatius H Sirikyi, Keisuke Kimura, Edward Gmakame, Sey Abdul-Rauf | Ano: 2019 | Database: pubmed

Resumo: Citrus aurantifolia (Christm.) Swingle (syn. C. MEDICA var. ACIDA Brandis) (family: Rutaceae) essential oil is one of the cheapest oils found in local markets. Although, it is generally accepted as non-toxic to vital organs and cells, majority of people are cynical about it usage. Herein, the present study reports the chemical composition and in vivo oral toxicity study of unripe C. aurantifolia essential oil found in Ghana. The toxicity of C. aurantifolia essential oil extract was investigated via oral administration using two methods: The acute toxicity single dose study (SDS) and the repeated dose method. The oil exhibited no acute toxicity but in the sub-chronic studies, the effects was dose and time-dependent. Chemical profile investigation of the oil showed 9 constituent of phytochemicals (Germacrene isomers (61.2%), Pineen (14%), Linalool dimmer (2.9%), Bornane (11%), Citral (2.9%), Anethole (1.5%), Anisole (1.1%), Safrole (0.3%) and Demitol (0.6%)). Histopathological studies revealed conditions such as necrosis, edema and inflammatory reaction in the liver, spleen and kidneys. Marginal upsurge of biochemical parameters above normal and elevated levels of lymphocytes (35.20-46.40 g/dL) demonstrated mild toxicity among the 100 mg/kg and 500 mg/kg dose groups at the sub-chronic stage. Low levels of hemoglobin (13.60 to 12.70 g/dL), MCV (34.20-24.0 fL), MCH (40.20-36.40 g/dL) along with high levels of liver enzymes confirmed the mild toxicity of the oil at sub-chronic stage. These results demonstrate that, despite consideration of lime essential oil as safe, it can have mild hematotoxic, nephrotoxic and hepatotoxic effects.

Resultados: N/A

Conclusão: N/A

Chemical profile and in vivo toxicity evaluation of unripe Citrus aurantifolia essential oil.

Autores: Christian K Adokoh, Du-Bois Asante, Desmond O Acheampong, Yohei Kotsuchibashi, Francis A Armah, Ignatius H Sirikyi, Keisuke Kimura, Edward Gmakame, Sey Abdul-Rauf | Ano: 2019 | Database: pubmed

Resumo: Citrus aurantifolia (Christm.) Swingle (syn. C. MEDICA var. ACIDA Brandis) (family: Rutaceae) essential oil is one of the cheapest oils found in local markets. Although, it is generally accepted as non-toxic to vital organs and cells, majority of people are cynical about it usage. Herein, the present study reports the chemical composition and in vivo oral toxicity study of unripe C. aurantifolia essential oil found in Ghana. The toxicity of C. aurantifolia essential oil extract was investigated via oral administration using two methods: The acute toxicity single dose study (SDS) and the repeated dose method. The oil exhibited no acute toxicity but in the sub-chronic studies, the effects was dose and time-dependent. Chemical profile investigation of the oil showed 9 constituent of phytochemicals (Germacrene isomers (61.2%), Pineen (14%), Linalool dimmer (2.9%), Bornane (11%), Citral (2.9%), Anethole (1.5%), Anisole (1.1%), Safrole (0.3%) and Demitol (0.6%)). Histopathological studies revealed conditions such as necrosis, edema and inflammatory reaction in the liver, spleen and kidneys. Marginal upsurge of biochemical parameters above normal and elevated levels of lymphocytes (35.20-46.40 g/dL) demonstrated mild toxicity among the 100 mg/kg and 500 mg/kg dose groups at the sub-chronic stage. Low levels of hemoglobin (13.60 to 12.70 g/dL), MCV (34.20-24.0 fL), MCH (40.20-36.40 g/dL) along with high levels of liver enzymes confirmed the mild toxicity of the oil at sub-chronic stage. These results demonstrate that, despite consideration of lime essential oil as safe, it can have mild hematotoxic, nephrotoxic and hepatotoxic effects.

Resultados: N/A

Conclusão: N/A