LOURO

In vitro acaricidal activity of essential oil and crude extracts of Laurus nobilis, (Lauraceae) grown in Tunisia, against arthropod ectoparasites of livestock and poultry: Hyalomma scupense and Dermanyssus gallinae.

Autores: Dhouha Alimi, Azhar Hajri, Selim Jallouli, Hichem Sebai | Ano: 2021 | Database: pubmed

Resumo: The current study assayed the toxicity of Laurus nobilis essential oil and crude extracts obtained using solvents of increasing polarity (cyclohexane, acetone and ethanol), on two ectoparasites of veterinary importance, i.e., Hyalomma scupense and Dermanyssus gallinae. The major components detected in bay laurel essential oil were dominated by 1.8-cineole (46.56 %), α-terpinenyl acetate (13.99 %), sabinene (7.69), α-pinene (5.75), linalool (5.50), methyleugenol (5.36 %) and β-pinene (3.97). The highest total phenolic and flavonoids contents were present in the ethalonic extract of L. nobilis leaves at an amount of 152.88 mg gallic acid equivalents per gram of dry weight (GAE/g DW) and 21.77 mg quercetin equivalent per gram of dry weight (QE/g DW), respectively. In vitro acaricidal effects of essensial oil and crude extract of L. nobilis against H. scupense were ascertained by adult immersion test of engorged females (AIT) and larval packet test (LPT) compared with a reference drug amitraz. The essential oil exhibited strong acaricidal activity against tick engorged female and inhibition of hatching eggs. After 24 h of exposure, at the highest tested concentration (100 mg/mL) essential oil induced 90.67 % mortality of H. scupense larvae (LC50 = 10.69 mg/mL). Otherwise, essential oil exhibited high acaricidal activity compared to extracts, and among the extract, the ethanolic extract revealed the highest acaricidal efficacy (81.27 % female mortality). Results from mite contact toxicity showed that essential oil and extracts from L. nobilis were toxic to D. gallinae. Bay essential oil was both more toxic to mites, and faster in exerting this toxicity than other tested crude extracts. L. nobilis essential oil concentration leaded to enhance mortality of D. gallinae reaching the highest (100 %) mortality at 12 h with a concentration of 320 mg/mL. While, ethanolic extract acheived this rate after 24 h of exposure at same concentration. Cyclohexanic extract showed weak acaricidal activity.

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Analgesic and anti-inflammatory activity of the leaf essential oil of Laurus nobilis Linn.

Autores: M Sayyah, G Saroukhani, A Peirovi, M Kamalinejad | Ano: 2003 | Database: pubmed

Resumo: The leaf essential oil of Laurus nobilis Linn. (Lauraceae) has been evaluated for antinociceptive and anti-inflammatory activities in mice and rats. The essential oil exhibited: (1) a significant analgesic effect in tail-flick and formalin tests; (2) a dose-dependent anti-inflammatory effect in the formalin-induced edema and (3) a moderate sedative effect at the anti-inflammatory doses. The analgesic and anti-inflammatory effect of the essential oil was comparable to reference analgesics and non-steroid anti-inflammatory drugs: morphine and piroxicam. Present results make the essential oil worthy of further investigations.

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Antibacterial and antibiofilm activities of polysaccharides, essential oil, and fatty oil extracted from Laurus nobilis growing in Lebanon.

Autores: Mohammad Chmit, Hussein Kanaan, Jean Habib, Mustafa Abbass, Ali Mcheik, Ali Chokr | Ano: 2014 | Database: pubmed

Resumo: To evaluate the antibacterial activity of the extracts of Laurus nobilis against three Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Staphylococcus epidermidis CIP 444) and two Gram-negative bacteria (Escherichia coli ATCC 35218 and Pseudomonas aeruginosa ATCC 27853). Also, the antibiofilm activity has been investigated against the biofilm produced by Staphylococcus epidermidis CIP 444.

Resultados: The results showed that alginate, fucoidan, fatty oils and essential oil have good antibacterial activities against the 5 bacterial strains, and a negligible biofilm eradication activity of fucoidan, laminaran, fatty oil, and essential oil was observed, but a promising biofilm eradication activity was obtained with alginate, which showed a reduced biofilm mass even at low concentration.

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Laurus nobilis L. Extracts against Paenibacillus larvae: Antimicrobial activity, antioxidant capacity, hygienic behavior and colony strength.

Autores: Natalia Jorgelina Fernández, Natalia Damiani, Enrique Arturo Podaza, Josefa Fabiana Martucci, Diana Fasce, Federico Quiroz, Pablo Ezequiel Meretta, Silvina Quintana, Martín Javier Eguaras, Liesel Brenda Gende | Ano: 2019 | Database: pubmed

Resumo: The aim of this work was to compare the antimicrobial activity against Paenibacillus larvae and the antioxidant capacity of two Laurus nobilis L. extracts obtained by different extraction methods. The hydroalcoholic extract was moreover added as supplementary diet to bees in field conditions to test behavioural effects and colony strength. Both laurel extracts were subjected to different phytochemical analysis to identify their bioactive compounds. Antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) determination by means the agar dilution method. The hydroalcoholic extract (HE) was able to inhibit the bacterial growth of all P. larvae strains, with 580 µg/mL mean value. This better antibacterial activity in relation to the essential oil (EO) could be explained by the presence of some phenolic compounds, such as flavonoids, evidenced by characteristic bands resulting from the Fourier Transform Infrared Spectroscopy (FTIR) analysis. Antioxidant activities of the extracts were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging ability and ferric reducing antioxidant power (FRAP) assays. The HE showed the highest antioxidant activity as measured by DPPH, with IC50 values of 257 ± 12 μg/mL. The FRAP assay method showed that the HE was 3-fold more effective reducing agent than the EO. When the bee colonies were supplied with laurel HE in sugar paste an improvement in their general condition was noticed, although neither the hygienic behavior nor the proportions of the breeding cells varied statistically due to the treatment. In conclusion, the inhibition power against P. larvae attributable to the phenolic compounds, the antioxidant capacity of the HE, and the non-lethal effects on adult honey bees on field trials suggest the HE of laurel as a promising substance for control American foulbrood disease.

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Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections.

Autores: A Merghni, H Marzouki, H Hentati, M Aouni, M Mastouri | Ano: 2015 | Database: pubmed

Resumo: Laurus nobilis L. is an aromatic herb with relevant medicinal properties due to its important chemical composition and its potential therapeutic effects. In this study, we investigate the chemical composition, the antibacterial and the antibiofilms activities of Tunisian L. nobilis L. essential oils against clinical Staphylococcus aureus strains.

Resultados: Our results revealed that GC-MS assay exhibited 1.8-Cineole, methyl eugenol and α-terpinyl acetate as the major compounds in the essential oils. Moreover, the essential oil from Sousse exhibited the best bactericidal activity (MICs values ranged from 3.91 to 15.62mgm-1). Furthermore, this oil showed a strong biofilm inhibition effect above 70%, from a low sub-inhibitory concentration (1/16MIC). MTT assay revealed that both essential oils displayed an excellent antibiofilm activity with eradication percentages ranging from 79.6±2.27 to 95.2±0.56.

Conclusão: Our finding demonstrated that L. nobilis L. essential oils are able to inhibit oral S. aureus strains with important antibiofilm efficacy. It could have a promising role in the prevention of oral diseases.

Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections.

Autores: A Merghni, H Marzouki, H Hentati, M Aouni, M Mastouri | Ano: 2015 | Database: pubmed

Resumo: Laurus nobilis L. is an aromatic herb with relevant medicinal properties due to its important chemical composition and its potential therapeutic effects. In this study, we investigate the chemical composition, the antibacterial and the antibiofilms activities of Tunisian L. nobilis L. essential oils against clinical Staphylococcus aureus strains.

Resultados: Our results revealed that GC-MS assay exhibited 1.8-Cineole, methyl eugenol and α-terpinyl acetate as the major compounds in the essential oils. Moreover, the essential oil from Sousse exhibited the best bactericidal activity (MICs values ranged from 3.91 to 15.62mgm-1). Furthermore, this oil showed a strong biofilm inhibition effect above 70%, from a low sub-inhibitory concentration (1/16MIC). MTT assay revealed that both essential oils displayed an excellent antibiofilm activity with eradication percentages ranging from 79.6±2.27 to 95.2±0.56.

Conclusão: Our finding demonstrated that L. nobilis L. essential oils are able to inhibit oral S. aureus strains with important antibiofilm efficacy. It could have a promising role in the prevention of oral diseases.

Antioxidant and antibacterial activity of essential oil and extracts of bay laurel Laurus nobilis Linnaeus (Lauraceae) from Portugal.

Autores: C Ramos, B Teixeira, I Batista, O Matos, C Serrano, N R Neng, J M F Nogueira, M L Nunes, A Marques | Ano: 2012 | Database: pubmed

Resumo: Laurus nobilis L. is an aromatic plant frequently used as a spice in Mediterranean cookery and as a traditional medicine for the treatment of several infectious diseases. The aim of this study was to characterise the antibacterial and antioxidant activities of bay laurel essential oil (EO), ethanolic extract (EE) and hot/cold aqueous extract (AE). The major components detected in bay laurel EO were eucalyptol (27.2%), α-terpinenyl acetate (10.2%), linalool (8.4%), methyleugenol (5.4%), sabinene (4.0%) and carvacrol (3.2%). The EO exhibited strong antibacterial activity against all tested foodborne spoilage and pathogenic bacteria, whereas this activity was less pronounced or even nonexistent in the EE and AE. In contrast, EO exhibited low antioxidant activity compared to extracts (EX), and among the EX, the hot AE revealed the highest antioxidant ability. The results show that bay laurel EO and its EX have potential as natural alternatives to synthetic food preservatives, in order to enhance food safety and increase food shelf life.

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Effective antibacterial and antioxidant properties of methanolic extract of Laurus nobilis seed oil.

Autores: Birgul Ozcan, Mari Esen, M Kemal Sangun, Arzu Coleri, Mahmut Caliskan | Ano: 2010 | Database: pubmed

Resumo: This study was carried out to determine the in vitro antimicrobial and antioxidant activities of the essential oil, seed oil, and methanolic extract of seed oil obtained from Laurus nobilis L. (Lauraceae). The methanolic extract of seed oil exhibited more effective antibacterial activity comparing to essential oil and seed oil, GC-MS analyses of the essential oil resulted in the identification of 25 compounds. 1.8-Cineol (44.72%), a-Terpinyl acetate (12.95%), Sabinene (12.82%) were the main components. The fatty acid composition was characterized with the high content of linoleic acid (40.79%) and lauric acid (38.08%). The 50% (IC50) inhibition activity of the essential oil on the free radical DPPH was determined as 94.655 mg ml(-1), whereas IC50 value of methanolic extract of seed oil was found unstable. In the case of the linoleic acid system, oxidation of linoleic acid was inhibited by essential oil and methanolic extract of seed oil, which showed 64.28 and 88.76% inhibition, respectively. The inhibition value of the methanolic extract of seed oil was quite close to the synthetic antioxidant BHT, 92.46% inhibition.

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Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens.

Autores: Itir Dadalioglu, Gulsun Akdemir Evrendilek | Ano: 2004 | Database: pubmed

Resumo: Chemical compositions and inhibitory effects of essential oils of Turkish oregano (Origanum minutiflorum O. Schwarz & P. H. Davis), bay laurel (Laurus nobilis L.), Spanish lavender (Lavandula stoechas subsp. stoechas L.), and fennel (Foeniculum vulgare Mill.) on Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus were determined. After the essential oils were applied on the foodborne pathogens at doses of 0 (control), 5, 10, 20, 30, 40, 50, and 80 microL/mL, the resultant numbers of cells surviving were counted. Results revealed that all essential oils exhibited a very strong antibacterial activity against the tested bacteria (P < 0.05). Gas chromatography-mass spectrophotometry analyses revealed that carvacrol (68.23%), 1,8-cineole (60.72%), fenchone (55.79%), and trans-anethole (85.63%) were the predominant constituents in Turkish oregano, bay laurel, Spanish lavender, and fennel essential oils, respectively.

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In Vitro Study of the Antifungal Activity of Essential Oils Obtained from Mentha spicata, Thymus vulgaris, and Laurus nobilis.

Autores: Abderrahmane Houicher, Hind Hechachna, Hanifa Teldji, Fatih Ozogul | Ano: 2016 | Database: pubmed

Resumo: The aim of this study was to determine the antifungal activity of the essential oils isolated from three aromatic plants against 13 filamentous fungal strains.

Resultados: In the present work, M. spicata essential oil exhibited the strongest activity against all tested fungi in which Fusarium graminearum, F.moniliforme, and Penicillium expansum were the most sensitive to mint oil with lower minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values of 2.5 μL mL-1 (v/v). Thymus vulgaris essential oil was less active compared to the oil of M. spicata. Aspergillus ochraceus was the most sensitive strain to thyme oil with MIC and MFC values of 2.5 and 5 μL mL-1, respectively. Thymus vulgaris essential oil also exhibited a moderate fungicidal effect against the tested fungi, except for A. niger (MFC >20 μL-1). L. nobilis essential oil showed a similar antifungal activity with thyme oil in which A. parasiticus was the most resistant strain to this oil (MFC >20 μL mL-1).

Conclusão: Our findings suggested the use of these essential oils as alternatives to synthetic fungicides in order to prevent pre-and post-harvest infections and ensure product safety.

Bomba Rice Conservation with a Natural Biofilm.

Autores: Josefa Roselló, Silvia Giménez, M Dolores Ibáñez, M Amparo Blázquez, M Pilar Santamarina | Ano: 2018 | Database: pubmed

Resumo: The chemical composition of commercial Syzygium aromaticum, Cinnamomum verum, and Laurus nobilis essential oils as well as their antifungal activity against four pathogenic fungi isolated from Mediterranean rice grains has been investigated. Eighty nine compounds accounting for between 98.5 and 99.4% of the total essential oil were identified. The phenylpropanoids eugenol (89.37 ± 0.29%) and eugenol (56.34 ± 0.41%), followed by eugenol acetate (19.48 ± 0.13%) were, respectively, the main compounds in clove and cinnamon essential oils, whereas large amounts of the oxygenated monoterpenes 1,8-cineole (58.07 ± 0.83%) and α-terpinyl acetate (13.05 ± 0.44%) were found in bay leaf essential oil. Clove and cinnamon oils showed the best antifungal activity results against all tested fungi. Against Alternaria alternata, clove essential oil displayed the best antifungal effect, whereas against Curvularia hawaiiensis, cinnamon essential oil was more active. Both essential oils showed a similar antifungal effect towards Fusarium proliferatum and Fusarium oxysporum. In vitro studies in inoculated rice grains showed that clove and cinnamon totally inhibited pathogenic fungal development after 30 days of incubation. In vivo studies showed that eugenol used with a polysaccharide such as agar-agar formed a fine coat which wraps the inoculated rice grains, creating a natural biofilm and reducing the development of all pathogenic fungi (80-95%) for 30 days.

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Prevention of Saprolegniasis in rainbow trout (Oncorhynchus mykiss) eggs using oregano (Origanum onites) and laurel (Laurus nobilis) essential oils.

Autores: Rahmi Can Özdemir, Yiğit Taştan, Kerim Güney | Ano: 2022 | Database: pubmed

Resumo: The present study investigated the antifungal effects of essential oils of oregano (Origanum onites) and laurel (Laurus nobilis) on Saprolegniasis, a disease that occurs in rainbow trout eggs during the incubation period. Oregano and laurel were ground after drying, and essential oils were obtained by water distillation method using a Clevenger apparatus. The essential oils were added to potato dextrose agar (PDA) at the rates of 1-1000 ppm, and the minimum inhibitory concentration (MIC) was determined as 250 ppm whereas the minimum lethal concentration (MLC) was determined to be 500 ppm for both plants. In the in vivo trials, fertilized eggs were treated with predetermined doses either by bathing during water hardening and incubation period or only during incubation period, and death rates were monitored during embryological development. The best larvae hatching rate was determined in 500 ppm oregano and 500 ppm laurel groups treated during water hardening plus daily as 82.11% and 79.87%, respectively. According to the results, it was determined that oregano and laurel essential oils exhibited better results in all doses compared with the negative control group, and 500 ppm dose had a better effect than the positive control group treated with formalin.

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Prevention of Saprolegniasis in rainbow trout (Oncorhynchus mykiss) eggs using oregano (Origanum onites) and laurel (Laurus nobilis) essential oils.

Autores: Rahmi Can Özdemir, Yiğit Taştan, Kerim Güney | Ano: 2022 | Database: pubmed

Resumo: The present study investigated the antifungal effects of essential oils of oregano (Origanum onites) and laurel (Laurus nobilis) on Saprolegniasis, a disease that occurs in rainbow trout eggs during the incubation period. Oregano and laurel were ground after drying, and essential oils were obtained by water distillation method using a Clevenger apparatus. The essential oils were added to potato dextrose agar (PDA) at the rates of 1-1000 ppm, and the minimum inhibitory concentration (MIC) was determined as 250 ppm whereas the minimum lethal concentration (MLC) was determined to be 500 ppm for both plants. In the in vivo trials, fertilized eggs were treated with predetermined doses either by bathing during water hardening and incubation period or only during incubation period, and death rates were monitored during embryological development. The best larvae hatching rate was determined in 500 ppm oregano and 500 ppm laurel groups treated during water hardening plus daily as 82.11% and 79.87%, respectively. According to the results, it was determined that oregano and laurel essential oils exhibited better results in all doses compared with the negative control group, and 500 ppm dose had a better effect than the positive control group treated with formalin.

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Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans.

Autores: E Mine Soylu, Soner Soylu, Sener Kurt | Ano: 2006 | Database: pubmed

Resumo: The aim of this study was to find an alternative to synthetic fungicides currently used in the control of devastating oomycete pathogen Phytophthora infestans, causal agent of late blight disease of tomato. Antifungal activities of essential oils obtained from aerial parts of aromatic plants such as oregano (Origanum syriacum var. bevanii), thyme (Thymbra spicata subsp. spicata), lavender (Lavandula stoechas subsp. stoechas), rosemary (Rosmarinus officinalis), fennel (Foeniculum vulgare), and laurel (Laurus nobilis), were investigated against P. infestans. Both contact and volatile phase effects of different concentrations of the essential oils used were determined by using two in vitro methods. Chemical compositions of the essential oils were also determined by GC-MS analysis. Major compounds found in essential oils of thyme, oregano, rosemary, lavender, fennel and laurel were carvacrol (37.9%), carvacrol (79.8), borneol (20.4%), camphor (20.2%), anethole (82.8%) and 1,8-cineole (35.5%), respectively. All essential oils were found to inhibit the growth of P. infestans in a dose-dependent manner. Volatile phase effect of oregano and thyme oils at 0.3 microg/ml air was found to completely inhibit the growth of P. infestans. Complete growth inhibition of pathogen by essential oil of fennel, rosemary, lavender and laurel was, however, observed at 0.4-2.0 microg/ml air concentrations. For the determination of the contact phase effects of the tested essential oils, oregano, thyme and fennel oils at 6.4 microg/ml were found to inhibit the growth of P. infestans completely. Essential oils of rosemary, lavender and laurel were inhibitory at relatively higher concentrations (12.8, 25.6, 51.2 microg/ml respectively). Volatile phase effects of essential oils were consistently found to be more effective on fungal growth than contact phase effect. Sporangial production was also inhibited by the essential oil tested. Light and scanning electron microscopic (SEM) observation on pathogen hyphae, exposed to both volatile and contact phase of oil, revealed considerable morphological alterations in hyphae such as cytoplasmic coagulation, vacuolations, hyphal shrivelling and protoplast leakage.

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Biological control of Citrus brown spot pathogen, "Alternaria alternata" by different essential oils.

Autores: Taycir Grati Affes, Synda Chenenaoui, Hassen Zemni, Majdi Hammami, Sarra Bachkouel, Wissem Aidi Wannes, Bouzid Nasraoui, Moufida Saidani Tounsi, Salma Lasram | Ano: 2022 | Database: pubmed

Resumo: The antifungal effects of laurel, myrtle and peppermint essential oils and their combinations were investigated in vitro on two strains of Alternaria alternata mycelial growth and in vivo on detached Citrus leaf disease incidence. Myrtle essential oil was rich in α-pinene and 1,8-cineole while peppermint essential oil in menthol and menthone. 1,8-Cineole was the main component of the essential oils from laurel, laurel + myrtle and peppermint + laurel. The combined peppermint and myrtle essential oil was characterized by the predominance of menthol and 1,8-cineole. All tested essential oils, incorporated in potato-dextrose agar, inhibited A. alternata mycelial growth and had a fungistatic effect at concentration 3 mg/mL of medium. A great synergism was detected between peppermint and laurel essential oils against the two strains of A. alternata. The combined laurel and peppermint essential oil reduced mycelial growth rates of inoculated detached leaves at concentration above 1.5 mg/mL.

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Antifungal, antitoxigenic, and antioxidant activities of the essential oil from laurel (Laurus nobilis L.): Potential use as wheat preservative.

Autores: Azem Belasli, Yamina Ben Miri, Malek Aboudaou, Lidia Aït Ouahioune, Luis Montañes, Agustín Ariño, Djamel Djenane | Ano: 2020 | Database: pubmed

Resumo: Essential oils (EOs) are widely used in the food industry as natural food preservatives to extend product shelf life and as flavoring agents. The aim of this work was to study the chemical profile of the EO from laurel (Laurus nobilis) and its antifungal, antitoxigenic, and antioxidant activities. The extractive yield of the EO from Algerian laurel was 1.13% being 1,8-cineole the most dominant compound (35.5%) by gas chromatography-mass spectrometry analysis. The values of minimum inhibitory concentration and minimum fungicidal concentration (MFC) against Aspergillus flavus were 1.75 and 2 mg/ml, respectively. The production of aflatoxin B1 was inhibited by EO concentrations between 0.25 mg/ml (15% decrease) and 1.50 mg/ml (86% decrease), and it was totally inhibited at the MFC value. The EO showed a wide antifungal spectrum against other species in a dose-dependent manner. In a food-model study, the L. nobilis EO showed remarkable efficacy in fumigated wheat grains, providing from 51.5% to 76.7% protection against A. flavus during 6-month storage. The L. nobilis EO showed good free radical scavenging activity by DPPH assay (IC50 value of 602 μg/ml) and moderate antioxidant activity in the β-carotene bleaching assay (46% inhibition of linoleic acid oxidation). The conclusions of this study justify future research for the application of EO from laurel as a natural preservative to improve food safety and extend shelf life by controlling spoilage and toxigenic molds as well as oxidative damage.

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Antimicrobial effect of laurel essential oil nanoemulsion on food-borne pathogens and fish spoilage bacteria.

Autores: Yesim Özogul, Nariman El Abed, Fatih Özogul | Ano: 2022 | Database: pubmed

Resumo: This research aimed to apply nanotechnology for nanoformulation of Laurus nobilis essential oil (EO) by ultrasonic emulsification method and characterization of nano-form: particle size, viscosity, polydispersity index, thermodynamic stability, and surface tension. The antimicrobial activity of laurel EO nanoemulsion (LEON) and laurel EO was also investigated against a panel of ten food-borne pathogens and fish spoilage bacteria. The GC-MS analysis of EO revealed that 1,8-Cineole was the main volatile compound. According to disc-diffusion results, LEON was more effective against Gram-positive pathogen bacteria of Staphylococcus aureus and Enterococcus faecalis than EO. Laurel oil demonstrated a higher inhibitory effect against fish spoilage bacteria (6.19 to 18.5 mm). The MICs values of LEON and laurel EO ranged from 6.25 to >25 mg/mL and from 1.56 to >25 mg/mL, respectively. Nanoemulsion and oil exhibited the best bactericidal activity against Pseudomonas luteola. Therefore, LEON can be developed as a natural antimicrobial agent in food industry.

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Use composite coating of chitosan-chia seed gum enriched with microliposomes of Bay laurel essential oil to increase the shelf life of quail fillets.

Autores: Motahareh Eslamian Amiri, Mohammad Ahmady, Peiman Ariaii, Leila Golestan, Azade Ghorbani-HasanSaraei | Ano: 2021 | Database: pubmed

Resumo: In this study, the effect of composite chitosan-chia seed coating (CH-CG) with Bay laurel (Laurus nobilis) essential oil (BE) in two forms including free and nanocapsulated on the shelf life of quail fillets during the 16-day refrigeration (4 ± 1℃) period was investigated. For this purpose, first, BE was extracted by Clevenger apparatus. Then, nanoliposomes BE were produced, and the properties of BE and nanoliposomes BE were investigated. In order to investigate the shelf life of quail, 6 treatments were produced including 1: control (C), 2: CH-CG, CH-CG+BE at 800 ppm, 3: CH-CG+BE at 1600 ppm, 4: CH-CG+nano BE at 800 ppm, 5: CH-CG+nano BE at 1600 ppm, and periodically chemical parameters (peroxide value, free fatty acid, total volatile basic nitrogen, texture firmness, and chewing ability) and microbial (total viable bacteria (TVC) and psychrotrophic bacteria), and the effect of different treatments on control in Escherichia coli and Staphylococcus aureus inoculated populations in quail was also investigated. The BE had high antioxidant and antimicrobial properties. The particle size and microencapsulation efficiency of BE nanoliposome were 98.3 nm and 75.95%, respectively. The results of chemical and microbial analysis showed that in general, the coating with essential oil slowed down the increasing trend of oxidation and microbial indices compared to the control treatment and nanocapsulation of essential oil has increased its antimicrobial and antioxidant properties (p < .05). At the end of storage period, in all tests, treatments of 3, 4, and 5 had the allowed microbial and chemical range and they also inhibited the growth of these bacteria (p < .05). Overall, considering the higher sensory score of treatment 4 and economic efficiency, it seems that this treatment can be used as a natural preservative in the meat industry.

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Phytochemical composition and antioxidant activity of Tunisian Laurus nobilis.

Autores: Wissal Dhifi, Sana Bellili, Sabrine Jazi, Soumaya Ben Nasr, Marc El-Beyrouthy, Wissem Mnif | Ano: 2018 | Database: pubmed

Resumo: We study the composition of Tunisian laurel leaves essential oil (EO), the fatty acid composition of laurel seed fixed oil and the total phenolics, flavonoids and tannins of laurel leaves methanolic extract. We also evaluated its free radical scavenging activity by the DPPH test. The predominant chemical class in Tunisian Laurus nobilis leaves EO was represented by oxygenated monoterpenes accounting for 64.29% of whole EO with the major compound was 1,8-cineole (46.8%). The predominant fatty acid was oleic acid (C18: 1) with an amount of 42.0%. Total polyphenols were present in the methanolic extract of Laurusnobilis leaves at an amount of 174.1 mg GAE.g-1dry matter. Total flavonoids and total tannins accounted respectively for 149.2mg CE.g-1 dry matter and 24.9mg CEg-1 dry matter. Furthermore, concerning free radical scavenging activity, Laurus nobilis leaves methanolic extract presented a significant IC50 (3mg/mL).

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Antioxidant and Anti-Atherogenic Activities of Essential Oils from Myrtus communis L. and Laurus nobilis L. in Rat.

Autores: Dyana Odeh, Nada Oršolić, Marija Berendika, Domagoj Đikić, Sandra Domjanić Drozdek, Sandra Balbino, Maja Repajić, Verica Dragović-Uzelac, Irena Landeka Jurčević | Ano: 2022 | Database: pubmed

Resumo: Essential oils (EOs) from aromatic and medicinal plants, such as myrtle (Myrtus communis L.) and Laurel (Laurus nobilis L.), are gaining popularity as a potential ingredient in functional foods and nutraceuticals. This study aims to investigate whether the essential oils (EOs) could be effective in weight control, antioxidative and antilipidemic status of rats by affecting microbiota and its enzymes activity and whether changes in intestinal enzyme activity affect the health of rats. The intragastric application of laurel and myrtle EOs to rats for two weeks affects weight loss, reduces glycolytic activity, lipid parameters (cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C)) and atherogenic indicators, leading to cardiovascular protection. Laurel EO can be an excellent candidate for the treatment of drug-induced obesity and related diseases, since it affects lipid metabolism in the liver and inhibits the enzymes responsible for the metabolism of carbohydrates into glucose in the digestive tract, leading to weight loss. In contrast, myrtle EO shows a better antioxidant capacity in most tissues, except kidneys, where it causes a pro-oxidative effect, compared to laurel EO. Myrtle EO increases the permeability and instability of the erythrocyte membrane, resulting in a loss of selectivity for the entry of toxic substances into the cell. On the other hand, myrtle EO leads to intestinal inflammation by reducing the number of probiotic bacteria and increasing Enterobacter.

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Use composite coating of chitosan-chia seed gum enriched with microliposomes of Bay laurel essential oil to increase the shelf life of quail fillets.

Autores: Motahareh Eslamian Amiri, Mohammad Ahmady, Peiman Ariaii, Leila Golestan, Azade Ghorbani-HasanSaraei | Ano: 2021 | Database: pubmed

Resumo: In this study, the effect of composite chitosan-chia seed coating (CH-CG) with Bay laurel (Laurus nobilis) essential oil (BE) in two forms including free and nanocapsulated on the shelf life of quail fillets during the 16-day refrigeration (4 ± 1℃) period was investigated. For this purpose, first, BE was extracted by Clevenger apparatus. Then, nanoliposomes BE were produced, and the properties of BE and nanoliposomes BE were investigated. In order to investigate the shelf life of quail, 6 treatments were produced including 1: control (C), 2: CH-CG, CH-CG+BE at 800 ppm, 3: CH-CG+BE at 1600 ppm, 4: CH-CG+nano BE at 800 ppm, 5: CH-CG+nano BE at 1600 ppm, and periodically chemical parameters (peroxide value, free fatty acid, total volatile basic nitrogen, texture firmness, and chewing ability) and microbial (total viable bacteria (TVC) and psychrotrophic bacteria), and the effect of different treatments on control in Escherichia coli and Staphylococcus aureus inoculated populations in quail was also investigated. The BE had high antioxidant and antimicrobial properties. The particle size and microencapsulation efficiency of BE nanoliposome were 98.3 nm and 75.95%, respectively. The results of chemical and microbial analysis showed that in general, the coating with essential oil slowed down the increasing trend of oxidation and microbial indices compared to the control treatment and nanocapsulation of essential oil has increased its antimicrobial and antioxidant properties (p < .05). At the end of storage period, in all tests, treatments of 3, 4, and 5 had the allowed microbial and chemical range and they also inhibited the growth of these bacteria (p < .05). Overall, considering the higher sensory score of treatment 4 and economic efficiency, it seems that this treatment can be used as a natural preservative in the meat industry.

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Antioxidant and antiproliferative activity of Laurus nobilis L. (Lauraceae) leaves and seeds essential oils against K562 human chronic myelogenous leukaemia cells.

Autores: Antoine M Saab, Rosa Tundis, Monica R Loizzo, Ilaria Lampronti, Monica Borgatti, Roberto Gambari, Federica Menichini, Fadi Esseily, Francesco Menichini | Ano: 2012 | Database: pubmed

Resumo: The antioxidant and antiproliferative activities of the essential oils from Laurus nobilis leaves and seeds in relation to their composition were analysed. The most abundant components of the leaf essential oil were 1,8-cineole, 1-p-menthen-8-ethyl acetate, linalool and sabinene, while the seed oil was characterised by β-ocimene, 1,8-cineole, α-pinene and β-pinene as main constituents. Both seed and leaf essential oils exhibited a scavenging effect on the DPPH radical, with IC₅₀ values of 66.1 and 53.5 µg mL⁻¹, respectively. The leaf essential oil showed the strongest antioxidant activity in the β-carotene/linoleic acid system, with an IC₅₀ value of 35.6 µg mL⁻¹ after 30 min of incubation. Both leaf and seed oils inhibited proliferation of the K562 tumour cell line with IC₅₀ values of 95 and 75 µg mL⁻¹, respectively. The L. nobilis leaf oil showed a percentage of erythroide differentiation of 15% at a concentration of 10 µg mL⁻¹. A value of 12% was found for the seed essential oil at a concentration of 50 µg mL⁻¹. When the oils were added to a suboptimal concentration of the commercial drug, cytosine arabinoside, a clear synergic effect was observed.

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Conclusão: N/A

Antioxidant and antiproliferative activity of Laurus nobilis L. (Lauraceae) leaves and seeds essential oils against K562 human chronic myelogenous leukaemia cells.

Autores: Antoine M Saab, Rosa Tundis, Monica R Loizzo, Ilaria Lampronti, Monica Borgatti, Roberto Gambari, Federica Menichini, Fadi Esseily, Francesco Menichini | Ano: 2012 | Database: pubmed

Resumo: The antioxidant and antiproliferative activities of the essential oils from Laurus nobilis leaves and seeds in relation to their composition were analysed. The most abundant components of the leaf essential oil were 1,8-cineole, 1-p-menthen-8-ethyl acetate, linalool and sabinene, while the seed oil was characterised by β-ocimene, 1,8-cineole, α-pinene and β-pinene as main constituents. Both seed and leaf essential oils exhibited a scavenging effect on the DPPH radical, with IC₅₀ values of 66.1 and 53.5 µg mL⁻¹, respectively. The leaf essential oil showed the strongest antioxidant activity in the β-carotene/linoleic acid system, with an IC₅₀ value of 35.6 µg mL⁻¹ after 30 min of incubation. Both leaf and seed oils inhibited proliferation of the K562 tumour cell line with IC₅₀ values of 95 and 75 µg mL⁻¹, respectively. The L. nobilis leaf oil showed a percentage of erythroide differentiation of 15% at a concentration of 10 µg mL⁻¹. A value of 12% was found for the seed essential oil at a concentration of 50 µg mL⁻¹. When the oils were added to a suboptimal concentration of the commercial drug, cytosine arabinoside, a clear synergic effect was observed.

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Conclusão: N/A

Effect of Laurus nobilis L. Essential Oil and its Main Components on α-glucosidase and Reactive Oxygen Species Scavenging Activity.

Autores: Serap Sahin Basak, Ferda Candan | Ano: 2013 | Database: pubmed

Resumo: The present study was designed to determine the effects of the essential oil of Laurus nobilis L. (Lauraceae) and its three main components on α-glucosidase and reactive oxygen species scavenging activity. The chemical composition of the essential oil from Laurus nobilis L. leaves was analyzed by GC/GC-MS and resulted in the identification of 29 compounds, representing 99.18% of the total oil. 1,8-cineole (68.82%), 1-(S)-α-pinene (6.94%), and R-(+)- limonene (3.04%) were determined to be the main components. The antioxidant features of the essential oil and its three main components were evaluated using inhibition of 2,2-diphenyl-1- picrylhydrazyl, hydroxyl, and superoxide radicals, inhibition of hydrogen peroxide and lipid peroxidation assays. The results show that the DPPH, hydroxyl, and superoxide radical as well as hydrogen peroxide scavenging activities of the essential oil are greater than the positive controls and the three main components of the oil when tested independently. The inhibition of lipid peroxidation by the oil occurred less frequently than with 1,8-cineole and R-(+)- limonene alone, but the effects were more pronounced than those seen with 1-(S)-α-pinene and the positive controls. An α-glucosidase inhibition assay was applied to evaluate the in-vitro antidiabetic activity of the essential oil. IC50-values were obtained for laurel essential oil, 1, 8-cineole, 1-(S)-α-pinene, and R-(+)-limonene: 1.748 μL/mL, 1.118 μL/mL, 1.420 μL/mL and 1.300 μL/mL, respectively. We also found that laurel essential oil and 1, 8-cineole inhibited the α-glucosidase competitively while 1-(S)-α-pinene and R-(+)-limonene were uncompetitive inhibitors.

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Conclusão: N/A

Chemical Composition, Antioxidant, Anti-Diabetic, Anti-Acetylcholinesterase, Anti-Inflammatory, and Antimicrobial Properties of Arbutus unedo L. and Laurus nobilis L. Essential Oils.

Autores: Samiah Hamad Al-Mijalli, Hanae Naceiri Mrabti, Hayat Ouassou, Rachid Flouchi, Emad M Abdallah, Ryan A Sheikh, Mohammed Merae Alshahrani, Ahmed Abdullah Al Awadh, Hicham Harhar, Nasreddine El Omari, Ahmed Qasem, Hamza Assaggaf, Naif Hesham Moursi, Abdelhakim Bouyahya, Monica Gallo, Moulay El Abbes Faouzi | Ano: 2022 | Database: pubmed

Resumo: The objectives of this work were to determine the phytochemical composition and antioxidant, anti-diabetic, antibacterial, anti-inflammatory, and anti-acetylcholinesterase properties of Arbutus unedo L. and Laurus nobilis L. EOs. The antioxidant effects were estimated using four complementary methods. In addition, the anti-diabetic activity was assessed by targeting three carbohydrate-hydrolyzing enzymes, namely α-amylase, α-glucosidase, and lipase. The anti-inflammatory and anti-acetylcholinesterase effects were evaluated by testing the inhibitory potential of both plants on lipo-oxygenase and acetylcholinesterase (AChE), respectively. The antimicrobial activity of these oils was evaluated using disc-diffusion, minimum inhibitory concentration (MIC), and minimum lethal concentration (MLC) tests. The chemical composition of L. nobilis essential oil (EO) was dominated by eucalyptol (36.40%), followed by α-terpineole (13.05%), α-terpinyl acetate (10.61%), linalool (10.34%), and northujane (5.74%). The main volatile compounds of A. unedo EOs were decenal (13.47%), α-terpineol (7.8%), and palmitic acid (6.00%). L. nobilis and A. unedo EOs inhibited α-amylase with IC50 values of 42.51 ± 0.012 and 102 ± 0.06 µg/mL, respectively. Moreover, both oils inhibited the activity of α-glucosidase (IC50 = 1.347 ± 0.021 µg/mL and IC50 = 76 ± 0.021 µg/mL) and lipase (IC50 = 21.23 ± 0.021 µg/mL and IC50 = 97.018 ± 0.012 µg/mL, respectively). In addition, L. nobilis EO showed an anti-AChE activity (IC50 = 89.44 ± 0.07 µg/mL) higher than that of A. unedo EO (IC50 = 378.57 ± 0.05 µg/mL). Regarding anti-inflammatory activity, in vitro assays showed that L. nobilis significantly inhibits (IC50 = 48.31 ± 0.07 μg/mL) 5-lipoxygenase compared to A. unedo (IC50 = 86.14 ± 0.05 μg/mL). This was confirmed in vivo via a notable inhibition of inflammation recorded after 6 h of treatment in both plants at a dose of 50 mg/kg. The microbiological results revealed that EOs from both plants inhibited the growth of all tested organisms except P. aeruginosa, with the highest antimicrobial effect for L. nobilis. The results of these tests showed that these two plants possess remarkable biological and pharmacological properties, explaining their medicinal effects and suggesting them as promising sources of natural drugs.

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Chemical Composition, Antioxidant, Anti-Diabetic, Anti-Acetylcholinesterase, Anti-Inflammatory, and Antimicrobial Properties of Arbutus unedo L. and Laurus nobilis L. Essential Oils.

Autores: Samiah Hamad Al-Mijalli, Hanae Naceiri Mrabti, Hayat Ouassou, Rachid Flouchi, Emad M Abdallah, Ryan A Sheikh, Mohammed Merae Alshahrani, Ahmed Abdullah Al Awadh, Hicham Harhar, Nasreddine El Omari, Ahmed Qasem, Hamza Assaggaf, Naif Hesham Moursi, Abdelhakim Bouyahya, Monica Gallo, Moulay El Abbes Faouzi | Ano: 2022 | Database: pubmed

Resumo: The objectives of this work were to determine the phytochemical composition and antioxidant, anti-diabetic, antibacterial, anti-inflammatory, and anti-acetylcholinesterase properties of Arbutus unedo L. and Laurus nobilis L. EOs. The antioxidant effects were estimated using four complementary methods. In addition, the anti-diabetic activity was assessed by targeting three carbohydrate-hydrolyzing enzymes, namely α-amylase, α-glucosidase, and lipase. The anti-inflammatory and anti-acetylcholinesterase effects were evaluated by testing the inhibitory potential of both plants on lipo-oxygenase and acetylcholinesterase (AChE), respectively. The antimicrobial activity of these oils was evaluated using disc-diffusion, minimum inhibitory concentration (MIC), and minimum lethal concentration (MLC) tests. The chemical composition of L. nobilis essential oil (EO) was dominated by eucalyptol (36.40%), followed by α-terpineole (13.05%), α-terpinyl acetate (10.61%), linalool (10.34%), and northujane (5.74%). The main volatile compounds of A. unedo EOs were decenal (13.47%), α-terpineol (7.8%), and palmitic acid (6.00%). L. nobilis and A. unedo EOs inhibited α-amylase with IC50 values of 42.51 ± 0.012 and 102 ± 0.06 µg/mL, respectively. Moreover, both oils inhibited the activity of α-glucosidase (IC50 = 1.347 ± 0.021 µg/mL and IC50 = 76 ± 0.021 µg/mL) and lipase (IC50 = 21.23 ± 0.021 µg/mL and IC50 = 97.018 ± 0.012 µg/mL, respectively). In addition, L. nobilis EO showed an anti-AChE activity (IC50 = 89.44 ± 0.07 µg/mL) higher than that of A. unedo EO (IC50 = 378.57 ± 0.05 µg/mL). Regarding anti-inflammatory activity, in vitro assays showed that L. nobilis significantly inhibits (IC50 = 48.31 ± 0.07 μg/mL) 5-lipoxygenase compared to A. unedo (IC50 = 86.14 ± 0.05 μg/mL). This was confirmed in vivo via a notable inhibition of inflammation recorded after 6 h of treatment in both plants at a dose of 50 mg/kg. The microbiological results revealed that EOs from both plants inhibited the growth of all tested organisms except P. aeruginosa, with the highest antimicrobial effect for L. nobilis. The results of these tests showed that these two plants possess remarkable biological and pharmacological properties, explaining their medicinal effects and suggesting them as promising sources of natural drugs.

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Conclusão: N/A

Antifungal Activity of Essential Oils Against Candida Species Isolated from Clinical Samples.

Autores: S Córdoba, W Vivot, W Szusz, G Albo | Ano: 2019 | Database: pubmed

Resumo: We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l-1 for essential oils and antifungal drugs, respectively. MIC50 and MIC90, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l-1), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l-1) and intermediate for C. glabrata (MIC≤ 32 mg l-1). The essential oils showed antifungal activity on Candida spp. tested with MIC90 values ranging from 0.8 to 800 mg l-1. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC90 0.8-0.16 mg l-1), and the least active was C. officinalis (MIC90 400-800 mg l-1). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.

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Effect of Laurus nobilis L. Essential Oil and its Main Components on α-glucosidase and Reactive Oxygen Species Scavenging Activity.

Autores: Serap Sahin Basak, Ferda Candan | Ano: 2013 | Database: pubmed

Resumo: The present study was designed to determine the effects of the essential oil of Laurus nobilis L. (Lauraceae) and its three main components on α-glucosidase and reactive oxygen species scavenging activity. The chemical composition of the essential oil from Laurus nobilis L. leaves was analyzed by GC/GC-MS and resulted in the identification of 29 compounds, representing 99.18% of the total oil. 1,8-cineole (68.82%), 1-(S)-α-pinene (6.94%), and R-(+)- limonene (3.04%) were determined to be the main components. The antioxidant features of the essential oil and its three main components were evaluated using inhibition of 2,2-diphenyl-1- picrylhydrazyl, hydroxyl, and superoxide radicals, inhibition of hydrogen peroxide and lipid peroxidation assays. The results show that the DPPH, hydroxyl, and superoxide radical as well as hydrogen peroxide scavenging activities of the essential oil are greater than the positive controls and the three main components of the oil when tested independently. The inhibition of lipid peroxidation by the oil occurred less frequently than with 1,8-cineole and R-(+)- limonene alone, but the effects were more pronounced than those seen with 1-(S)-α-pinene and the positive controls. An α-glucosidase inhibition assay was applied to evaluate the in-vitro antidiabetic activity of the essential oil. IC50-values were obtained for laurel essential oil, 1, 8-cineole, 1-(S)-α-pinene, and R-(+)-limonene: 1.748 μL/mL, 1.118 μL/mL, 1.420 μL/mL and 1.300 μL/mL, respectively. We also found that laurel essential oil and 1, 8-cineole inhibited the α-glucosidase competitively while 1-(S)-α-pinene and R-(+)-limonene were uncompetitive inhibitors.

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Conclusão: N/A

Phytochemical profile and anthelmintic effects of Laurus nobilis essential oil against the ovine nematode Haemonchus contortus and the murine helminth model Heligmosomoides polygyrus.

Autores: Essia Sebai, Amel Abidi, Hayet Benyedem, Mokhtar Dhibi, Ines Hammemi, Hafidh Akkari | Ano: 2022 | Database: pubmed

Resumo: Small ruminant production in tropical and temperate countries faced substantial anthelmintic resistance due to the intensive use of commercial anthelmintic drugs. Therefore, alternative treatments including natural bioactive compounds with anthelmintic potential have been investigated looking for its successfully use in the parasite control. In the present study, we describe the chemical profile of Laurus nobilis essential oil (EO), the in vitro anthelmintic activity of L. nobilis EO against Haemonchus contortus and its in vivo anthelmintic effect against the murine helminth parasite model Heligmosomoides polygyrus. Chromatographic profile of L. nobilis (EO) extracted from the leaves of L. nobilis have shown the presence of monterpens 1,8-cineol (Eucalyptol) (29.47%), D-Limonène (18.51%) and Linalool (10.84%) in high fractions. The in vitro anthelmintic potential was expressed by an ovicidal effect against H. contortus egg hatching with inhibition value of 1.72 mg/mL and 87.5% of immobility of adult worms after 8 h of exposure to 4 mg/mL of L. nobilis EO. Regarding, the in vivo anthelmintic potential, L. nobilis (EO) at 2400 mg/kg bw completely eliminated the egg output of H. polygyrus after 7 days of oral treatment, together with a 79.2% of reduction in total worm counts. Based on the obtained results, L. nobilis EO showed promising in vitro and in vivo anthelmintic capacities against gastrointestinal parasites.

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Conclusão: N/A

Phytochemical profile and anthelmintic effects of Laurus nobilis essential oil against the ovine nematode Haemonchus contortus and the murine helminth model Heligmosomoides polygyrus.

Autores: Essia Sebai, Amel Abidi, Hayet Benyedem, Mokhtar Dhibi, Ines Hammemi, Hafidh Akkari | Ano: 2022 | Database: pubmed

Resumo: Small ruminant production in tropical and temperate countries faced substantial anthelmintic resistance due to the intensive use of commercial anthelmintic drugs. Therefore, alternative treatments including natural bioactive compounds with anthelmintic potential have been investigated looking for its successfully use in the parasite control. In the present study, we describe the chemical profile of Laurus nobilis essential oil (EO), the in vitro anthelmintic activity of L. nobilis EO against Haemonchus contortus and its in vivo anthelmintic effect against the murine helminth parasite model Heligmosomoides polygyrus. Chromatographic profile of L. nobilis (EO) extracted from the leaves of L. nobilis have shown the presence of monterpens 1,8-cineol (Eucalyptol) (29.47%), D-Limonène (18.51%) and Linalool (10.84%) in high fractions. The in vitro anthelmintic potential was expressed by an ovicidal effect against H. contortus egg hatching with inhibition value of 1.72 mg/mL and 87.5% of immobility of adult worms after 8 h of exposure to 4 mg/mL of L. nobilis EO. Regarding, the in vivo anthelmintic potential, L. nobilis (EO) at 2400 mg/kg bw completely eliminated the egg output of H. polygyrus after 7 days of oral treatment, together with a 79.2% of reduction in total worm counts. Based on the obtained results, L. nobilis EO showed promising in vitro and in vivo anthelmintic capacities against gastrointestinal parasites.

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Antibacterial activity of essential oils extracted from the unique Chinese spices cassia bark, bay fruits and cloves.

Autores: Chunling Jiang, Jiaju Hong, Jing Meng, Jie Ou, Qingchao Xie, Yingjie Pan, Yong Zhao, Haiquan Liu | Ano: 2022 | Database: pubmed

Resumo: Spices are widely used in daily life such as diet and have certain activity. Especially in China, spices have been mainly used as condiments for thousands of years in order to improve the sensory quality of food; in addition, they and their derivatives can also be used as preservatives. In this study, three spices with unique Chinese characteristics widely used were selected: cassia bark (bark of Cinnamomum camphora Presl), bay fruits (Laurus nobilis), and cloves (Syzygiumaromaticum). The main components and antibacterial ability of these three spices were analyzed by simulated extraction method. Through headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) analysis, it was determined that the main active compounds in the essential oils of cassia bark, bay fruits and cloves were cinnamaldehyde (78.11%), cinnamaldehyde (61.78%) and eugenol (75.23%), respectively. The agar plate diffusion test and the simulated food culture medium experiment confirmed that the essential oils extracted from the three flavors have antibacterial effects on Listeria monocytogenes, Listeria innocua, Listeria welshimeri, Listeria ivanovii, Listeria grayi and Vibrio parahaemolyticus. The antibacterial activity of different strains has different optimal extraction conditions. Generally speaking, cinnamon essential oil has the strongest antibacterial activity, while laurel fruit has the lowest antibacterial activity. The study proved the antibacterial activity of these three Chinese-specific spices and provided some new ideas and methods for the subsequent research and preparation of natural food additives and food antibacterial agents.

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Conclusão: N/A

Antibacterial activity of essential oils extracted from the unique Chinese spices cassia bark, bay fruits and cloves.

Autores: Chunling Jiang, Jiaju Hong, Jing Meng, Jie Ou, Qingchao Xie, Yingjie Pan, Yong Zhao, Haiquan Liu | Ano: 2022 | Database: pubmed

Resumo: Spices are widely used in daily life such as diet and have certain activity. Especially in China, spices have been mainly used as condiments for thousands of years in order to improve the sensory quality of food; in addition, they and their derivatives can also be used as preservatives. In this study, three spices with unique Chinese characteristics widely used were selected: cassia bark (bark of Cinnamomum camphora Presl), bay fruits (Laurus nobilis), and cloves (Syzygiumaromaticum). The main components and antibacterial ability of these three spices were analyzed by simulated extraction method. Through headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) analysis, it was determined that the main active compounds in the essential oils of cassia bark, bay fruits and cloves were cinnamaldehyde (78.11%), cinnamaldehyde (61.78%) and eugenol (75.23%), respectively. The agar plate diffusion test and the simulated food culture medium experiment confirmed that the essential oils extracted from the three flavors have antibacterial effects on Listeria monocytogenes, Listeria innocua, Listeria welshimeri, Listeria ivanovii, Listeria grayi and Vibrio parahaemolyticus. The antibacterial activity of different strains has different optimal extraction conditions. Generally speaking, cinnamon essential oil has the strongest antibacterial activity, while laurel fruit has the lowest antibacterial activity. The study proved the antibacterial activity of these three Chinese-specific spices and provided some new ideas and methods for the subsequent research and preparation of natural food additives and food antibacterial agents.

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Conclusão: N/A

Chemical composition and antibacterial activity of the essential oil of Laurus nobilis leaves.

Autores: Bekhti Nabila, Alessandra Piras, Belabdelli Fouzia, Danilo Falconieri, Guenaoui Kheira, Firdaous-Faiza Fedoul, Sekkal-Rahal Majda | Ano: 2022 | Database: pubmed

Resumo: Chemical composition determination and in vitro antibacterial effect of Essential Oil (EO) of Laurus nobilis leaves grown North West Algerian conditions were investigated. Antibacterial activity was evaluated by the technique of the paper disc diffusion. Extractions were carried out with Clevenger apparatus and EO compositions were investigated by gas chromatography coupled to mass spectrometry (GC-MS) analysis. The oxygenated monoterpenes (MO) were the class with the highest contents, represent 59% of the EO. A total of twenty-seven of these compounds were identified, the major components were 1,8-cineole (30.1%), α-terpynil acetate (21.6%), methyl eugenol (16.9%) of the total oil. The highest antibacterial power of the EO tested was recorded in Streptococcus faecalis and Staphylococcus aureus with the zone diameter of inhibition 13.6 and 11.2 mm respectively and a minimal inhibitory concentration (MIC) of 0.25 mg/mL for both these two strains.

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Comparative study on the chemical composition of laurel (Laurus nobilis L.) leaves from Greece and Georgia and the antibacterial activity of their essential oil.

Autores: Galina Stefanova, Tanya Girova, Velizar Gochev, Magdalena Stoyanova, Zhana Petkova, Albena Stoyanova, Valtcho D Zheljazkov | Ano: 2020 | Database: pubmed

Resumo: Laurel (Laurus nobilis L.) is a plant species from Lauraceae family, and is native to the Mediterranean region. The goal of this study was to compare chemical composition of laurel leaves and antibacterial activity of its essential oil (EO) from wild-grown trees in Greece and Georgia. The laurel leaves from the two native habitats had dissimilar concentrations of phenolic acids. Of the conjugated flavonols and flavons, kaempferol (1981.3 μg/g) and apigenin (1433.6 μg/g) were the major representatives in the leaves from Greece, while luteolin (839.1 μg/g) and kaempferol (688.1 μg/g) were the major ones in the leaves from Georgia, respectively. The EO content was 1.42% and 4.54% in the leaves from Greece and Georgia, respectively. The main EO constituents of the Greek laurel plants were 1,8-cineole (30.8%), α-terpinyl acetate (14.9%), α-terpineol (8.0%), sabinene (7.9%), and terpinen-4-ol (6.0%). The main EO constituents of the Georgian laurel plants were 1,8-cineole (29.2%), α-terpinyl acetate (22.6%), sabinene (12.2%), and methyleugenol (8.1%). The EO antimicrobial activities against 20 microorganisms were determined. Among the Gram-positive bacteria, the Enterococcus faecalis strain was the most sensitive, followed by Staphylococcus aureus ATCC 6538. Among the Candida species, C. albicans ATCC 10231 was the most sensitive to the laurel leaf EOs.

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Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens.

Autores: Itir Dadalioglu, Gulsun Akdemir Evrendilek | Ano: 2004 | Database: pubmed

Resumo: Chemical compositions and inhibitory effects of essential oils of Turkish oregano (Origanum minutiflorum O. Schwarz & P. H. Davis), bay laurel (Laurus nobilis L.), Spanish lavender (Lavandula stoechas subsp. stoechas L.), and fennel (Foeniculum vulgare Mill.) on Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus were determined. After the essential oils were applied on the foodborne pathogens at doses of 0 (control), 5, 10, 20, 30, 40, 50, and 80 microL/mL, the resultant numbers of cells surviving were counted. Results revealed that all essential oils exhibited a very strong antibacterial activity against the tested bacteria (P < 0.05). Gas chromatography-mass spectrophotometry analyses revealed that carvacrol (68.23%), 1,8-cineole (60.72%), fenchone (55.79%), and trans-anethole (85.63%) were the predominant constituents in Turkish oregano, bay laurel, Spanish lavender, and fennel essential oils, respectively.

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Conclusão: N/A

Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens.

Autores: Itir Dadalioglu, Gulsun Akdemir Evrendilek | Ano: 2004 | Database: pubmed

Resumo: Chemical compositions and inhibitory effects of essential oils of Turkish oregano (Origanum minutiflorum O. Schwarz & P. H. Davis), bay laurel (Laurus nobilis L.), Spanish lavender (Lavandula stoechas subsp. stoechas L.), and fennel (Foeniculum vulgare Mill.) on Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus were determined. After the essential oils were applied on the foodborne pathogens at doses of 0 (control), 5, 10, 20, 30, 40, 50, and 80 microL/mL, the resultant numbers of cells surviving were counted. Results revealed that all essential oils exhibited a very strong antibacterial activity against the tested bacteria (P < 0.05). Gas chromatography-mass spectrophotometry analyses revealed that carvacrol (68.23%), 1,8-cineole (60.72%), fenchone (55.79%), and trans-anethole (85.63%) were the predominant constituents in Turkish oregano, bay laurel, Spanish lavender, and fennel essential oils, respectively.

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Biological activity evaluation of the oils from Laurus nobilis of Tunisia and Algeria extracted by supercritical carbon dioxide.

Autores: Hanen Marzouki, Abdelhamid Khaldi, Rachid Chamli, Sadok Bouzid, Alessandra Piras, Danilo Falconieri, Bruno Marongiu | Ano: 2009 | Database: pubmed

Resumo: Isolation of essential oil from dried and ground leaves of Laurus nobilis L. from Algeria and Tunisia were obtained with two different methods: by means of classical hydrodistillation (HD) and by using carbon dioxide in the supercritical state (SFE). The effect of extraction pressure on the yield and composition of the L. nobilis volatile oil is examined. The best extraction conditions were as follows: pressure, 9.0 MPa; temperature, 323.15 K; 4 h of extraction and carbon dioxide flow, Phi = 1.2 kg h(-1). Waxes were entrapped in the first separator set at 9.0 MPa and 263.15 K. The oil was recovered in the second separator working at 2.0 bar and 288.15 K. GC/MS analysis of the leaves' volatile oil revealed that it mainly consisted of: 1,8-cineole, linalool, alpha-terpinyl acetate, methyl eugenol and sabinene. The comparison with the hydrodistilled oil did not reveal any big difference. The collection of samples at different extraction times during supercritical extraction allowed us to monitor the change of the oil composition. On the matrice exhaust owing to a SFE at 9.0 MPa, one further extraction at 323.15 K and 24.0 MPa was performed to obtain a mixture of compounds with higher molecular weight. Finally, the antibacterial and antifungal activity of the various extracts has been assayed.

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Chemical Composition and Antimicrobial Activity of Laurus nobilis L. Essential Oils from Bulgaria.

Autores: Hafize Fidan, Galina Stefanova, Iliana Kostova, Stanko Stankov, Stanka Damyanova, Albena Stoyanova, Valtcho D Zheljazkov | Ano: 2019 | Database: pubmed

Resumo: Laurel, Laurus nobilis L. is an evergreen plant belonging to the Lauraceae family, native to Southern Europe and the Mediterranean area. This is the first report on the composition and bioactivity of laurel essential oil (EO) from Bulgaria. The oil yield was 0.78%, 0.80%, and 3.25% in the fruits, twigs, and leaves, respectively. The main constituents in the fruit EO were 1,8-cineole (33.3%), α-terpinyl acetate (10.3%), α-pinene (11.0%), β-elemene (7.5%), sabinene (6.3%), β-phellandrene (5.2%), bornyl acetate (4.4%), and camphene (4.3%); those in the twig EO were 1,8-cineole (48.5%), α-terpinyl acetate (13.1%), methyl eugenol (6.6%), β-linalool (3.8%), β-pinene (3.4%), sabinene (3.3%) and terpinene-4-ol (3.3%); and the ones in the leaf EO were 1,8-cineole (41.0%), α-terpinyl acetate (14.4%), sabinene (8.8%), methyl eugenole (6.0%), β-linalool (4.9%), and α-terpineol (3.1%). The antibacterial and antifungal properties of laurel EOs were examined according to the agar well diffusion method. The leaf EO showed antibacterial and antifungal activities against almost all strains of the microorganisms tested, whereas the twig EO was only able to inhibit Staphylococcus aureus. Pseudomonas aeruginosa ATCC 9027 and Escherichia coli ATCC 8739 were the bacterial strains that showed the highest resistance to the laurel EO. The results can benefit the EO industry and biopesticide development.

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Anticonvulsant activity of the leaf essential oil of Laurus nobilis against pentylenetetrazole- and maximal electroshock-induced seizures.

Autores: M Sayyah, J Valizadeh, M Kamalinejad | Ano: 2002 | Database: pubmed

Resumo: The leaf essential oil of Laurus nobilis Linn., Lauraceae, which has been used as an antiepileptic remedy in Iranian traditional medicine, was evaluated for anticonvulsant activity against experimental seizures. The essential oil protected mice against tonic convulsions induced by maximal electroshock and especially by pentylenetetrazole. Components responsible for this effect may be methyleugenol, eugenol and pinene present in the essential oil. At anticonvulsant doses, the essential oil produced sedation and motor impairment. This effect seems to be related in part to cineol, eugenol and methyleugenol. Although the essential oil had an acceptable acute toxicity, further studies are required before any absolute conclusions can be drawn.

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The chemical composition of some Lauraceae essential oils and their antifungal activities.

Autores: A Simić, M D Soković, M Ristić, S Grujić-Jovanović, J Vukojević, P D Marin | Ano: 2004 | Database: pubmed

Resumo: The antifungal activity of Aniba rosaeodora, Laurus nobilis, Sassafras albidum and Cinnamomum zeylanicum essential oils were investigated against 17 micromycetes. Among the tested fungal species were food poisoning, spoilage fungi, plant and animal pathogens. In order to determine fungistatic and fungicidal concentrations (MIC and MFC) macrodilution and microdilution tests were used. Linalool was the main component in the essential oil of A. rosaeodora, while 1.8-cineole was dominant in L. nobilis. In sassafras essential oil safrole was the major component and in the oil of C. zeylanicum the main component was trans-cinnamaldehyde. The essential oil of cinnamon showed the strongest antifungal activity.

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Antifungal activity, mode of action and anti-biofilm effects of Laurus nobilis Linnaeus essential oil against Candida spp.

Autores: Larissa Rangel Peixoto, Pedro Luiz Rosalen, Gabriela Lacet Silva Ferreira, Irlan Almeida Freires, Fabíola Galbiatti de Carvalho, Lúcio Roberto Castellano, Ricardo Dias de Castro | Ano: 2017 | Database: pubmed

Resumo: The present study demonstrated the antifungal potential of the chemically characterized essential oil (EO) of Laurus nobilis L. (bay laurel) against Candida spp. biofilm adhesion and formation, and further established its mode of action on C. albicans.

Resultados: The MIC and MFC values of the EO ranged from (250 to 500) μg/mL. The MIC values increased in the presence of sorbitol (osmotic protector) and ergosterol, which indicates that the EO may affect cell wall biosynthesis and membrane ionic permeability, respectively. At 2 MIC the EO disrupted initial adhesion of C. albicans biofilms (p<0.05) and affected biofilm formation with no difference compared to nystatin (p>0.05). When applied for 1min, every 8h, for 24h and 48h, the EO reduced the amount of C. albicans mature biofilm with no difference in relation to nystatin (p>0.05). The phytochemical analysis identified isoeugenol as the major compound (53.49%) in the sample.

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Antifungal Activity of Essential Oils Against Candida Species Isolated from Clinical Samples.

Autores: S Córdoba, W Vivot, W Szusz, G Albo | Ano: 2019 | Database: pubmed

Resumo: We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l-1 for essential oils and antifungal drugs, respectively. MIC50 and MIC90, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l-1), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l-1) and intermediate for C. glabrata (MIC≤ 32 mg l-1). The essential oils showed antifungal activity on Candida spp. tested with MIC90 values ranging from 0.8 to 800 mg l-1. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC90 0.8-0.16 mg l-1), and the least active was C. officinalis (MIC90 400-800 mg l-1). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.

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Antifungal Activity of Essential Oils Against Candida Species Isolated from Clinical Samples.

Autores: S Córdoba, W Vivot, W Szusz, G Albo | Ano: 2019 | Database: pubmed

Resumo: We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l-1 for essential oils and antifungal drugs, respectively. MIC50 and MIC90, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l-1), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l-1) and intermediate for C. glabrata (MIC≤ 32 mg l-1). The essential oils showed antifungal activity on Candida spp. tested with MIC90 values ranging from 0.8 to 800 mg l-1. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC90 0.8-0.16 mg l-1), and the least active was C. officinalis (MIC90 400-800 mg l-1). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.

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Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans.

Autores: E Mine Soylu, Soner Soylu, Sener Kurt | Ano: 2006 | Database: pubmed

Resumo: The aim of this study was to find an alternative to synthetic fungicides currently used in the control of devastating oomycete pathogen Phytophthora infestans, causal agent of late blight disease of tomato. Antifungal activities of essential oils obtained from aerial parts of aromatic plants such as oregano (Origanum syriacum var. bevanii), thyme (Thymbra spicata subsp. spicata), lavender (Lavandula stoechas subsp. stoechas), rosemary (Rosmarinus officinalis), fennel (Foeniculum vulgare), and laurel (Laurus nobilis), were investigated against P. infestans. Both contact and volatile phase effects of different concentrations of the essential oils used were determined by using two in vitro methods. Chemical compositions of the essential oils were also determined by GC-MS analysis. Major compounds found in essential oils of thyme, oregano, rosemary, lavender, fennel and laurel were carvacrol (37.9%), carvacrol (79.8), borneol (20.4%), camphor (20.2%), anethole (82.8%) and 1,8-cineole (35.5%), respectively. All essential oils were found to inhibit the growth of P. infestans in a dose-dependent manner. Volatile phase effect of oregano and thyme oils at 0.3 microg/ml air was found to completely inhibit the growth of P. infestans. Complete growth inhibition of pathogen by essential oil of fennel, rosemary, lavender and laurel was, however, observed at 0.4-2.0 microg/ml air concentrations. For the determination of the contact phase effects of the tested essential oils, oregano, thyme and fennel oils at 6.4 microg/ml were found to inhibit the growth of P. infestans completely. Essential oils of rosemary, lavender and laurel were inhibitory at relatively higher concentrations (12.8, 25.6, 51.2 microg/ml respectively). Volatile phase effects of essential oils were consistently found to be more effective on fungal growth than contact phase effect. Sporangial production was also inhibited by the essential oil tested. Light and scanning electron microscopic (SEM) observation on pathogen hyphae, exposed to both volatile and contact phase of oil, revealed considerable morphological alterations in hyphae such as cytoplasmic coagulation, vacuolations, hyphal shrivelling and protoplast leakage.

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Antifungal Activity and Chemical Composition of Seven Essential Oils to Control the Main Seedborne Fungi of Cucurbits.

Autores: Marwa Moumni, Gianfranco Romanazzi, Basma Najar, Luisa Pistelli, Hajer Ben Amara, Kaies Mezrioui, Olfa Karous, Ikbal Chaieb, Mohamed Bechir Allagui | Ano: 2021 | Database: pubmed

Resumo: Essential oils represent novel alternatives to application of synthetic fungicides to control against seedborne pathogens. This study investigated seven essential oils for in vitro growth inhibition of the main seedborne pathogens of cucurbits. Cymbopogon citratus essential oil completely inhibited mycelial growth of Stagonosporopsis cucurbitacearum and Alternaria alternata at 0.6 and 0.9 mg/mL, respectively. At 1 mg/mL, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and two Origanum majorana essential oils inhibited mycelia growth of A. alternata by 54%, 71%, 68%, 36%, 90%, and 74%, respectively. S. cucurbitacearum mycelia growth was more sensitive to Lavandula essential oils, with inhibition of ~74% at 1 mg/mL. To determine the main compounds in these essential oils that might be responsible for this antifungal activity, they were analyzed by gas chromatography-mass spectrometry (GC-MS). C. citratus essential oil showed cirtal as its main constituent, while L. dentata and L. nobilis essential oils showed eucalyptol. The M. alternifolia and two O. majorana essential oils had terpinen-4-ol as the major constituent, while for L. hybrida essential oil, this was linalool. Thus, in vitro, these essential oils can inhibit the main seedborne fungi of cucurbits, with future in vivo studies now needed to confirm these activities.

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Bomba Rice Conservation with a Natural Biofilm.

Autores: Josefa Roselló, Silvia Giménez, M Dolores Ibáñez, M Amparo Blázquez, M Pilar Santamarina | Ano: 2018 | Database: pubmed

Resumo: The chemical composition of commercial Syzygium aromaticum, Cinnamomum verum, and Laurus nobilis essential oils as well as their antifungal activity against four pathogenic fungi isolated from Mediterranean rice grains has been investigated. Eighty nine compounds accounting for between 98.5 and 99.4% of the total essential oil were identified. The phenylpropanoids eugenol (89.37 ± 0.29%) and eugenol (56.34 ± 0.41%), followed by eugenol acetate (19.48 ± 0.13%) were, respectively, the main compounds in clove and cinnamon essential oils, whereas large amounts of the oxygenated monoterpenes 1,8-cineole (58.07 ± 0.83%) and α-terpinyl acetate (13.05 ± 0.44%) were found in bay leaf essential oil. Clove and cinnamon oils showed the best antifungal activity results against all tested fungi. Against Alternaria alternata, clove essential oil displayed the best antifungal effect, whereas against Curvularia hawaiiensis, cinnamon essential oil was more active. Both essential oils showed a similar antifungal effect towards Fusarium proliferatum and Fusarium oxysporum. In vitro studies in inoculated rice grains showed that clove and cinnamon totally inhibited pathogenic fungal development after 30 days of incubation. In vivo studies showed that eugenol used with a polysaccharide such as agar-agar formed a fine coat which wraps the inoculated rice grains, creating a natural biofilm and reducing the development of all pathogenic fungi (80-95%) for 30 days.

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Antifungal activity, mode of action and anti-biofilm effects of Laurus nobilis Linnaeus essential oil against Candida spp.

Autores: Larissa Rangel Peixoto, Pedro Luiz Rosalen, Gabriela Lacet Silva Ferreira, Irlan Almeida Freires, Fabíola Galbiatti de Carvalho, Lúcio Roberto Castellano, Ricardo Dias de Castro | Ano: 2017 | Database: pubmed

Resumo: The present study demonstrated the antifungal potential of the chemically characterized essential oil (EO) of Laurus nobilis L. (bay laurel) against Candida spp. biofilm adhesion and formation, and further established its mode of action on C. albicans.

Resultados: The MIC and MFC values of the EO ranged from (250 to 500) μg/mL. The MIC values increased in the presence of sorbitol (osmotic protector) and ergosterol, which indicates that the EO may affect cell wall biosynthesis and membrane ionic permeability, respectively. At 2 MIC the EO disrupted initial adhesion of C. albicans biofilms (p<0.05) and affected biofilm formation with no difference compared to nystatin (p>0.05). When applied for 1min, every 8h, for 24h and 48h, the EO reduced the amount of C. albicans mature biofilm with no difference in relation to nystatin (p>0.05). The phytochemical analysis identified isoeugenol as the major compound (53.49%) in the sample.

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Chemical Composition and Antimicrobial Activity of Essential Oils from Three Mediterranean Plants against Eighteen Pathogenic Bacteria and Fungi.

Autores: Soukaina Razzouk, Mouaad Amine Mazri, Lamya Jeldi, Bacem Mnasri, Lahcen Ouahmane, Mohamed Najib Alfeddy | Ano: 2022 | Database: pubmed

Resumo: The chemical composition and antimicrobial activity of essential oils (EOs) obtained from three medicinal plants of the Moroccan flora were evaluated. The chemical composition of EOs of Thymus leptobotrys, Laurus nobilis and Syzygium aromaticum was determined using a gas chromatograph coupled with mass spectrometry. Carvacrol (75.05%) was the main constituent of T. leptobotrys EOs, while 1,8-cineole (31.48%) and eugenol (82.16%) were the predominant components of L. nobilis and S. aromaticum EOs, respectively. The antimicrobial activity of the EOs was evaluated qualitatively and quantitatively against 18 microbial strains pathogenic to humans by using the disc diffusion method, and by measuring the minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC). The EOs of T. leptobotrys were the most active against the strains tested, with inhibitory zone values ranging from 7.00 to 45.00 mm, and MIC and MMC values ranging from 0.312 to 80.00 mg/mL. In many cases, these EOs exhibited higher antibacterial and antifungal activities than the chemical compounds ciprofloxacin and fluconazole, respectively. This high antimicrobial activity can be ascribed to their richness in carvacrol. The EOs of T. leptobotrys, L. nobilis, and S. aromaticum could be considered a promising alternative to replace chemical antimicrobials, and a readily available natural source of bioactive compounds.

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Antimicrobial effects of Turkish propolis, pollen, and laurel on spoilage and pathogenic food-related microorganisms.

Autores: Osman Erkmen, Mehmet Musa Ozcan | Ano: 2008 | Database: pubmed

Resumo: The antimicrobial activities of propolis extract, pollen extract, and essential oil of laurel (Laurus nobilis L.) at concentrations from 0.02% to 2.5% (vol/vol) were investigated on bacteria (Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Yersinia enterocolitica, Enterococcus faecalis, and Listeria monocytogenes), yeasts (Saccharomyces cerevisiae and Candida rugosa), and molds (Aspergillus niger and Rhizopus oryzae). Pollen has no antimicrobial effects on the bacteria and fungi tested in the concentrations used. Propolis showed a bactericidal effect at 0.02% on B. cereus and B. subtilis, at 1.0% on S. aureus and E. faecalis, and at 0.2% on L. monocytogenes. The minimum inhibitory concentration of propolis for fungi was 2.5%. Propolis and laurel were ineffective against E. coli and S. typhimurium at the concentrations tested. The results showed that the antimicrobial activity were concentration dependent. Propolis and essential oil of laurel may be used as biopreservative agents in food processing and preservation.

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Antimicrobial effect of laurel essential oil nanoemulsion on food-borne pathogens and fish spoilage bacteria.

Autores: Yesim Özogul, Nariman El Abed, Fatih Özogul | Ano: 2022 | Database: pubmed

Resumo: This research aimed to apply nanotechnology for nanoformulation of Laurus nobilis essential oil (EO) by ultrasonic emulsification method and characterization of nano-form: particle size, viscosity, polydispersity index, thermodynamic stability, and surface tension. The antimicrobial activity of laurel EO nanoemulsion (LEON) and laurel EO was also investigated against a panel of ten food-borne pathogens and fish spoilage bacteria. The GC-MS analysis of EO revealed that 1,8-Cineole was the main volatile compound. According to disc-diffusion results, LEON was more effective against Gram-positive pathogen bacteria of Staphylococcus aureus and Enterococcus faecalis than EO. Laurel oil demonstrated a higher inhibitory effect against fish spoilage bacteria (6.19 to 18.5 mm). The MICs values of LEON and laurel EO ranged from 6.25 to >25 mg/mL and from 1.56 to >25 mg/mL, respectively. Nanoemulsion and oil exhibited the best bactericidal activity against Pseudomonas luteola. Therefore, LEON can be developed as a natural antimicrobial agent in food industry.

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Chemical composition and antimicrobial activity of essential oils from selected herbs cultivated in the South of Brazil against food spoilage and foodborne pathogens/ Composição química e atividade antimicrobiana de óleos essenciais de plantas selecionadas cultivadas no Sul do Brasil contra micro-organismos patogênicos e deteriorantes de alimentos

Autores: Silveira, Sheila Mello da; Cunha Júnior, Anildo; Scheuermann, Gerson Neudí; Secchi, Fábio Luiz; Vieira, Cleide Rosana Werneck | Ano: 2012 | Database: LILACS

Resumo: The chemical composition of 10 selected plant essential oils obtained by steam distillation was determined by GC and GC/MS. The antimicrobial activity of the essential oils was screened against 12 important food-related bacterial strains by agar disc-diffusion assay. MIC and MBC were determined for the essential oils that presented the highest activity in the agar disc-diffusion test. The most active essential oils against the tested bacteria were, in descending order, lemongrass (Cymbopogon flexuosus), basil (Ocimum basilicum), oregano (Origanum vulgare), cinnamon leaf (Cinnamomum zeylanicum), and laurel (Laurus nobilis). Except for S. Typhimurium, the tested bateria were inhibited at MIC values lower or equal to 0.62mg mL-1 by lemongrass (Cymbopogon flexuosus) essential oil. Yersinia enterocolitica presented the highest sensitivity to all essential oils tested (CMI≤0.62mg mL-1). There was a significant correlation (P<0.05) between oxygenated monoterpenes levels in the essential oils and MIC and MBC values against Escherichia coli. Results showed that the evaluated essential oils present high potential as natural preservatives.

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Laurus nobilis L. Extracts against Paenibacillus larvae: Antimicrobial activity, antioxidant capacity, hygienic behavior and colony strength.

Autores: Natalia Jorgelina Fernández, Natalia Damiani, Enrique Arturo Podaza, Josefa Fabiana Martucci, Diana Fasce, Federico Quiroz, Pablo Ezequiel Meretta, Silvina Quintana, Martín Javier Eguaras, Liesel Brenda Gende | Ano: 2019 | Database: pubmed

Resumo: The aim of this work was to compare the antimicrobial activity against Paenibacillus larvae and the antioxidant capacity of two Laurus nobilis L. extracts obtained by different extraction methods. The hydroalcoholic extract was moreover added as supplementary diet to bees in field conditions to test behavioural effects and colony strength. Both laurel extracts were subjected to different phytochemical analysis to identify their bioactive compounds. Antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) determination by means the agar dilution method. The hydroalcoholic extract (HE) was able to inhibit the bacterial growth of all P. larvae strains, with 580 µg/mL mean value. This better antibacterial activity in relation to the essential oil (EO) could be explained by the presence of some phenolic compounds, such as flavonoids, evidenced by characteristic bands resulting from the Fourier Transform Infrared Spectroscopy (FTIR) analysis. Antioxidant activities of the extracts were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging ability and ferric reducing antioxidant power (FRAP) assays. The HE showed the highest antioxidant activity as measured by DPPH, with IC50 values of 257 ± 12 μg/mL. The FRAP assay method showed that the HE was 3-fold more effective reducing agent than the EO. When the bee colonies were supplied with laurel HE in sugar paste an improvement in their general condition was noticed, although neither the hygienic behavior nor the proportions of the breeding cells varied statistically due to the treatment. In conclusion, the inhibition power against P. larvae attributable to the phenolic compounds, the antioxidant capacity of the HE, and the non-lethal effects on adult honey bees on field trials suggest the HE of laurel as a promising substance for control American foulbrood disease.

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Antimicrobial effects of Turkish propolis, pollen, and laurel on spoilage and pathogenic food-related microorganisms.

Autores: Osman Erkmen, Mehmet Musa Ozcan | Ano: 2008 | Database: pubmed

Resumo: The antimicrobial activities of propolis extract, pollen extract, and essential oil of laurel (Laurus nobilis L.) at concentrations from 0.02% to 2.5% (vol/vol) were investigated on bacteria (Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Yersinia enterocolitica, Enterococcus faecalis, and Listeria monocytogenes), yeasts (Saccharomyces cerevisiae and Candida rugosa), and molds (Aspergillus niger and Rhizopus oryzae). Pollen has no antimicrobial effects on the bacteria and fungi tested in the concentrations used. Propolis showed a bactericidal effect at 0.02% on B. cereus and B. subtilis, at 1.0% on S. aureus and E. faecalis, and at 0.2% on L. monocytogenes. The minimum inhibitory concentration of propolis for fungi was 2.5%. Propolis and laurel were ineffective against E. coli and S. typhimurium at the concentrations tested. The results showed that the antimicrobial activity were concentration dependent. Propolis and essential oil of laurel may be used as biopreservative agents in food processing and preservation.

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Comparison of Chemical Composition and Biological Properties of Essential Oils Obtained by Hydrodistillation and Steam Distillation of Laurus nobilis L.

Autores: Kristýna Řebíčková, Tomáš Bajer, David Šilha, Karel Ventura, Petra Bajerová | Ano: 2020 | Database: pubmed

Resumo: The purpose of this study was to compare the yield, chemical composition, antimicrobial and antioxidant properties of essential oils isolated from leaves of Laurus nobilis L. by two different distillation methods. The essential oils isolated by hydrodistillation (HD) and steam distillation (SD) were analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). Hydrodistillation produced a yield of 0.95 ± 0.06% which is slightly higher than yield obtained by steam distillation 0.79 ± 0.07%. Seventy three compounds in the bay leaves oil obtained by steam distillation were identified while in essential oil obtained by hydrodistillation were identified only 54 compounds. The antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method. Antimicrobial activity of obtained essential oils was evaluated by disc diffusion method in comparison with several chosen antimicrobials. The antimicrobial activity was tested on five microorganisms - Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans. In general, oils produced by steam distillation had higher antimicrobial and antioxidant activities than hydrodistillation extracts. It seems that hydrodistillation is better for higher yield while steam distillation is better to use for more quality oils with stronger biological properties.

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Phytochemical composition and antioxidant activity of Tunisian Laurus nobilis.

Autores: Wissal Dhifi, Sana Bellili, Sabrine Jazi, Soumaya Ben Nasr, Marc El-Beyrouthy, Wissem Mnif | Ano: 2018 | Database: pubmed

Resumo: We study the composition of Tunisian laurel leaves essential oil (EO), the fatty acid composition of laurel seed fixed oil and the total phenolics, flavonoids and tannins of laurel leaves methanolic extract. We also evaluated its free radical scavenging activity by the DPPH test. The predominant chemical class in Tunisian Laurus nobilis leaves EO was represented by oxygenated monoterpenes accounting for 64.29% of whole EO with the major compound was 1,8-cineole (46.8%). The predominant fatty acid was oleic acid (C18: 1) with an amount of 42.0%. Total polyphenols were present in the methanolic extract of Laurusnobilis leaves at an amount of 174.1 mg GAE.g-1dry matter. Total flavonoids and total tannins accounted respectively for 149.2mg CE.g-1 dry matter and 24.9mg CEg-1 dry matter. Furthermore, concerning free radical scavenging activity, Laurus nobilis leaves methanolic extract presented a significant IC50 (3mg/mL).

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Suggestions on the Contribution of Methyl Eugenol and Eugenol to Bay Laurel (Laurus nobilis L.) Essential Oil Preservative Activity through Radical Scavenging.

Autores: Nikolaos Nenadis, Maria Papapostolou, Maria Z Tsimidou | Ano: 2021 | Database: pubmed

Resumo: The present study examined the radical scavenging potential of the two benzene derivatives found in the bay laurel essential oil (EO), namely methyl eugenol (MEug) and eugenol (Eug), theoretically and experimentally to make suggestions on their contribution to the EO preservative activity through such a mechanism. Calculation of appropriate molecular indices widely used to characterize chain-breaking antioxidants was carried out in the gas and liquid phases (n-hexane, n-octanol, methanol, water). Experimental evidence was based on the DPPH• scavenging assay applied to pure compounds and a set of bay laurel EOs chemically characterized with GC-MS/FID. Theoretical calculations suggested that the preservative properties of both compounds could be exerted through a radical scavenging mechanism via hydrogen atom donation. Eug was predicted to be of superior efficiency in line with experimental findings. Pearson correlation and partial least square regression analyses of the EO antioxidant activity values vs. % composition of individual volatiles indicated the positive contribution of both compounds to the radical scavenging activity of bay laurel EOs. Eug, despite its low content in bay laurel EOs, was found to influence the most the radical scavenging activity of the latter.

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Laurus nobilis, Zingiber officinale and Anethum graveolens Essential Oils: Composition, Antioxidant and Antibacterial Activities against Bacteria Isolated from Fish and Shellfish.

Autores: Mejdi Snuossi, Najla Trabelsi, Sabrine Ben Taleb, Ameni Dehmeni, Guido Flamini, Vincenzo De Feo | Ano: 2016 | Database: pubmed

Resumo: Several bacterial strains were isolated from wild and reared fish and shellfish. The identification of these strains showed the dominance of the Aeromonas hydrophila species in all seafood samples, followed by Staphylococcus spp., Vibrio alginolyticus, Enterobacter cloacae, Klebsiella ornithinolytica, Klebsiella oxytoca and Serratia odorifera. The isolates were studied for their ability to produce exoenzymes and biofilms. The chemical composition of the essential oils from Laurus nobilis leaves, Zingiber officinale rhizomes and Anethum graveolens aerial parts was studied by GC and GC/MS. The essential oils' antioxidant and antibacterial activities against the isolated microorganisms were studied. Low concentrations of the three essential oils were needed to inhibit the growth of the selected bacteria and the lowest MBCs values were obtained for the laurel essential oil. The selected essential oils can be used as a good natural preservative in fish food due to their antioxidant and antibacterial activities.

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Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells.

Autores: Jelnar Z Al-Kalaldeh, Rana Abu-Dahab, Fatma U Afifi | Ano: 2010 | Database: pubmed

Resumo: Medicinal plants and culinary herbs have gained importance in the last decade as cytotoxic and antitumor agents. We hypothesized that some of the commonly used spices with reported antimicrobial activity might have antiproliferative activity. In the present study, selected spices used in Jordan were chemically analyzed and investigated for their antiproliferative activity to the adenocarcinoma of breast cell line (MCF7). The composition of the essential oils of Laurus nobilis L, Origanum syriacum L, Origanum vulgare L, and Salvia triloba L was analyzed by gas chromatography-mass spectrometry. The antiproliferative activities of the hydrodistilled volatile oils and the crude ethanol and water extracts were evaluated using the sulphorhodamine B assay. 1,8-Cineol was the major constituent in the hydrodistilled oils of both plants, L nobilis and S triloba, with concentrations of 40.91% and 45.16%, respectively. The major constituent of O syriacum was the carvacrol (47.10%), whereas that of O vulgare was trans-sabinene hydrate (27.19%). The ethanol crude extracts of O syriacum, L nobilis, and S triloba showed antiproliferative activity to MCF7 with IC(50) values 6.40, 24.49, and 25.25 microg/mL, respectively. However, none of the hydrodistilled essential oils of the tested plant species or their aqueous extracts demonstrated cytotoxic activity.

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Molecular size fractions of bay leaf (Laurus nobilis) exhibit differentiated regulation of colorectal cancer cell growth in vitro.

Autores: Louise Bennett, Mahinda Abeywardena, Sharon Burnard, Santina Forsyth, Richard Head, Kerryn King, Glen Patten, Peter Watkins, Roderick Williams, Dimitrios Zabaras, Trevor Lockett | Ano: 2013 | Database: pubmed

Resumo: Numerous in vitro studies using solvent or aqueous extracts of raw dietary plant material have demonstrated modulation of colon cancer cell growth and apoptosis and effects on immune and nonimmune pathways of inflammation. We have developed a generic, 3-staged food-compatible process involving heating for conversion of dietary plants into food ingredients and report results on potential colon cancer-regulating properties of processed forms of Bay leaf (Laurus nobilis). In vitro studies demonstrated inhibition of cancer cell growth by processed Bay leaf products in HT-29, HCT-116, Caco-2, and SW-480 human cancer cell lines, which were accompanied by variable levels of elevated apoptosis. Bay leaf also exerted moderate inhibition of cycloxygenase 2 and 5 lipoxygenase enzymatic activity. In addition, these extracts significantly downregulated interferon-γ production in T helper Type 1-stimulated whole blood from healthy donors. Furthermore, size fractionation of the extracts revealed that antiproliferative and proapoptotic activities were associated with low mass (primarily polyphenolics and essential oils) and high mass (primarily proteins including polyphenol oxidase) chemical classes, respectively. Bay leaf exerted in vitro bioactivity that might be relevant to protecting against early events in sporadic colorectal cancer, with potential for further optimization of bioactivity by size-based fractionation.

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Phytochemical analysis and in vitro antiviral activities of the essential oils of seven Lebanon species.

Autores: Monica R Loizzo, Antoine M Saab, Rosa Tundis, Giancarlo A Statti, Francesco Menichini, Ilaria Lampronti, Roberto Gambari, Jindrich Cinatl, Hans Wilhelm Doerr | Ano: 2008 | Database: pubmed

Resumo: The chemical composition of the essential oils of Laurus nobilis, Juniperus oxycedrus ssp. oxycedrus, Thuja orientalis, Cupressus sempervirens ssp. pyramidalis, Pistacia palaestina, Salvia officinalis, and Satureja thymbra was determined by GC/MS analysis. Essential oils have been evaluated for their inhibitory activity against SARS-CoV and HSV-1 replication in vitro by visually scoring of the virus-induced cytopathogenic effect post-infection. L. nobilis oil exerted an interesting activity against SARS-CoV with an IC(50) value of 120 microg/ml and a selectivity index (SI) of 4.16. This oil was characterized by the presence of beta-ocimene, 1,8-cineole, alpha-pinene, and beta-pinene as the main constituents. J. oxycedrus ssp. oxycedrus oil, in which alpha-pinene and beta-myrcene were the major constituents, revealed antiviral activity against HSV-1 with an IC(50) value of 200 microg/ml and a SI of 5.

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Antimicrobial activity and chemical characterization of Laurel essential oil/ Atividade antimicrobiana e caracterização química do óleo essencial de Louro/ Actividad antimicrobiana y caracterización química del aceite esencial de Laurel

Autores: Nicolao, Juliana Olívia; Bellaver, Franciana Aparecida Volpato; Kirst, Volmir; Radin, Daniel; Haro, Marcelo Mendes de; Nicolao, André Alois; Santos, Elis Regina Alchieri dos; Millezi, Alessandra Farias | Ano: 2021 | Database: LILACS

Resumo: The factors that arouse interest in the study of essential oils as biocidal agents are numerous, such as the fact that they have antibacterial, antifungal, insecticidal, antioxidant, anti-inflammatory and larvicidal properties. The objective of this work was to evaluate the antimicrobial activity, in vitro, of the laurel (Laurus nobilis L) essential oil on the growth of pathogenic bacteria Salmonella enterica serovar Typhimurium ATCC 14028 and Staphylococcus aureus ATCC 25923, at different exposure times, as well as to perform the chemical characterization. Twenty compounds were identified and quantified, representing 96.57% of the total composition. The class of oxygenated monoterpenes represented the majority class of the essential oil, with 1,8-cineol (33.8%) as the substance found in greater quantity, followed by linalool (17.79%). The third constituent in greater quantity was sabinene (12.23%), belonging to the group of monoterpene hydrocarbons. Terpinyl acetate (9.41%) was also considered to be quantitatively representative. Laurel essential oil showed bacteriostatic activity against S. Typhimurium ATCC 14028 and S. aureus ATCC 25923.(AU)

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Laurel essential oil: biological activities and application for semolina preservation against the red flour beetle Tribolium castaneum (Tenebrionidae).

Autores: Soumaya Haouel-Hamdi, Abir Soltani, Mohamed Ben Hamedou, Olfa Bachrouch, Maha Ben Abada, Chokri Messaoud, Mohamed Ali El Annabi, Jazia Sriti Eljazi, Emna Boushih, Majdi Hammami, Ferid Limam, Jouda Mediouni Ben Jemâa | Ano: 2022 | Database: pubmed

Resumo: The phytochemical composition of Laurus nobilis essential oil and their anticholinesterase, antioxidant, and insecticidal potential were studied. Also, the oil volatile fraction was compared in semolina at the beginning and after storage periods. For that, a headspace solid-phase-microextraction analysis (HS-SPME) coupled with gas chromatography and mass spectrometry was undertaken. Significant quantitative and qualitative differences of the oil volatile fraction were detected according to storage periods and occupation space ratios. Additionally, anti-acetylcholinesterase activity of L. nobilis oil against T. castaneum adults was evaluated. Results revealed that insecticide activity varied according to storage duration and occupation space. Besides, the oil acts on acetylcholine hydrolysis by inhibiting the activity of acetylcholinesterase. These results highlighted that L. nobilis essential oil may be recommended as an eco-friendly alternative for preserving semolina during storage.

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Laurus nobilis, Zingiber officinale and Anethum graveolens Essential Oils: Composition, Antioxidant and Antibacterial Activities against Bacteria Isolated from Fish and Shellfish.

Autores: Mejdi Snuossi, Najla Trabelsi, Sabrine Ben Taleb, Ameni Dehmeni, Guido Flamini, Vincenzo De Feo | Ano: 2016 | Database: pubmed

Resumo: Several bacterial strains were isolated from wild and reared fish and shellfish. The identification of these strains showed the dominance of the Aeromonas hydrophila species in all seafood samples, followed by Staphylococcus spp., Vibrio alginolyticus, Enterobacter cloacae, Klebsiella ornithinolytica, Klebsiella oxytoca and Serratia odorifera. The isolates were studied for their ability to produce exoenzymes and biofilms. The chemical composition of the essential oils from Laurus nobilis leaves, Zingiber officinale rhizomes and Anethum graveolens aerial parts was studied by GC and GC/MS. The essential oils' antioxidant and antibacterial activities against the isolated microorganisms were studied. Low concentrations of the three essential oils were needed to inhibit the growth of the selected bacteria and the lowest MBCs values were obtained for the laurel essential oil. The selected essential oils can be used as a good natural preservative in fish food due to their antioxidant and antibacterial activities.

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Phytochemical analysis and in vitro antiviral activities of the essential oils of seven Lebanon species.

Autores: Monica R Loizzo, Antoine M Saab, Rosa Tundis, Giancarlo A Statti, Francesco Menichini, Ilaria Lampronti, Roberto Gambari, Jindrich Cinatl, Hans Wilhelm Doerr | Ano: 2008 | Database: pubmed

Resumo: The chemical composition of the essential oils of Laurus nobilis, Juniperus oxycedrus ssp. oxycedrus, Thuja orientalis, Cupressus sempervirens ssp. pyramidalis, Pistacia palaestina, Salvia officinalis, and Satureja thymbra was determined by GC/MS analysis. Essential oils have been evaluated for their inhibitory activity against SARS-CoV and HSV-1 replication in vitro by visually scoring of the virus-induced cytopathogenic effect post-infection. L. nobilis oil exerted an interesting activity against SARS-CoV with an IC(50) value of 120 microg/ml and a selectivity index (SI) of 4.16. This oil was characterized by the presence of beta-ocimene, 1,8-cineole, alpha-pinene, and beta-pinene as the main constituents. J. oxycedrus ssp. oxycedrus oil, in which alpha-pinene and beta-myrcene were the major constituents, revealed antiviral activity against HSV-1 with an IC(50) value of 200 microg/ml and a SI of 5.

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Combined Effect of Chitosan Coating and Laurel Essential Oil (Laurus nobilis) on the Microbiological, Chemical, and Sensory Attributes of Water Buffalo Meat.

Autores: Lydia K Karakosta, Kornilia A Vatavali, Ioanna S Kosma, Anastasia V Badeka, Michael G Kontominas | Ano: 2022 | Database: pubmed

Resumo: The combined effect of chitosan coating (CHI) and laurel essential oil (LEO) on the shelf-life extension of water buffalo meat stored under aerobic packaging conditions at 4 °C was investigated. Microbiological, physicochemical, and sensory attributes were monitored over an 18-day storage period. Microbiological data indicated that the (CHI) coating along with (LEO) was the most efficient among treatments in reducing populations of bacteria by 3.2 log cfu/g on day 6 of storage (p < 0.05). pH values of meat varied between 6.04 and 6.21, while thiobarbituric acid (TBA) values were equal to or less than 2.12 mg malondialdehyde/kg throughout storage. The colour parameter L* and a* values decreased, while b* values increased during storage (p < 0.05). Taste proved to be a more sensitive sensory attribute than odour. Based on sensory and microbiological data, product shelf life was approximately 5−6 days for control samples, 7−8 days for samples treated with (LEO), 12 days for samples treated with (CHI), and 13−14 days for samples treated with (CHI + LEO).

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Effect of guar gum coatings containing essential oils on shelf life and nutritional quality of green-unripe mangoes during low temperature storage.

Autores: Ayeza Naeem, Tanveer Abbas, Tahira Mohsin Ali, Abid Hasnain | Ano: 2018 | Database: pubmed

Resumo: This study investigated the effect of treatment of guar gum coating coupled with essential oils. Harvested unripe green mangoes (UGM) were preserved using edible coatings containing essential oils of Nigella sativa, Coriandrum sativum, Foeniculum vulgare and Laurus nobilis derived using two different solvents (methanol and ethanol) and stored at refrigeration temperature (10°C, 80-85% relative humidity). Physiological and biochemical parameters that assess the quality of fruits were determined. Microbiological analysis was also performed at the start and end of shelf life. Generally, it was observed that ethanolic essential oils supplemented coatings conferred a greater retention of fruit quality as compared to both controls. Bacterial counts were significantly reduced in fruits that were coated with ethanolic essential oil edible coatings. Secondly, the coatings supplemented with ethanolic and methanolic essential oils extended shelf life up to 24days whereas treated and untreated control decayed after 10 and 6days respectively (P<0.05). These results suggested the application of these edible coatings for preservation of unripe green mangoes during cold storage.

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Combined Effect of Chitosan Coating and Laurel Essential Oil (Laurus nobilis) on the Microbiological, Chemical, and Sensory Attributes of Water Buffalo Meat.

Autores: Lydia K Karakosta, Kornilia A Vatavali, Ioanna S Kosma, Anastasia V Badeka, Michael G Kontominas | Ano: 2022 | Database: pubmed

Resumo: The combined effect of chitosan coating (CHI) and laurel essential oil (LEO) on the shelf-life extension of water buffalo meat stored under aerobic packaging conditions at 4 °C was investigated. Microbiological, physicochemical, and sensory attributes were monitored over an 18-day storage period. Microbiological data indicated that the (CHI) coating along with (LEO) was the most efficient among treatments in reducing populations of bacteria by 3.2 log cfu/g on day 6 of storage (p < 0.05). pH values of meat varied between 6.04 and 6.21, while thiobarbituric acid (TBA) values were equal to or less than 2.12 mg malondialdehyde/kg throughout storage. The colour parameter L* and a* values decreased, while b* values increased during storage (p < 0.05). Taste proved to be a more sensitive sensory attribute than odour. Based on sensory and microbiological data, product shelf life was approximately 5−6 days for control samples, 7−8 days for samples treated with (LEO), 12 days for samples treated with (CHI), and 13−14 days for samples treated with (CHI + LEO).

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Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Chemical Composition of Essential Oils.

Autores: Anđela Miljanović, Ana Bielen, Dorotea Grbin, Zvonimir Marijanović, Martina Andlar, Tonči Rezić, Sunčica Roca, Igor Jerković, Dražen Vikić-Topić, Maja Dent | Ano: 2020 | Database: pubmed

Resumo: The effect of different hydrodistillation pretreatments, namely, reflux extraction, reflux extraction with the addition of cell wall-degrading enzymes, and ultrasound, on the yield and chemical composition of essential oils of sage, bay laurel, and rosemary was examined. All pretreatments improved essential oil yield compared to no-pretreatment control (40-64% yield increase), while the oil quality remained mostly unchanged (as shown by statistical analysis of GC-MS results). However, enzyme-assisted reflux extraction pretreatment did not significantly outperform reflux extraction (no-enzyme control), suggesting that the observed yield increase was mostly a consequence of reflux extraction and enzymatic activity had only a minute effect. Thus, we show that ultrasound and reflux extraction pretreatments are beneficial in the production of essential oils of selected Mediterranean plants, but the application of enzymes has to be carefully re-evaluated.

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Antiglycation, comparative antioxidant potential, phenolic content and yield variation of essential oils from 19 exotic and endemic medicinal plants.

Autores: F Mahomoodally, Z Aumeeruddy-Elalfi, Katharigatta N Venugopala, M Hosenally | Ano: 2019 | Database: pubmed

Resumo: The antioxidant potential, antiglycation, and total phenolic content of essential oils (EOs) extracted from 19 medicinal plants were assessed. The variation in yield of the EOs with respect to altitude and season was also studied. The antioxidant potential of Pimenta dioica (L.) Merr., Psiadia terebinthina A.J. Scott, Laurus nobilis L., Piper betle L., and Citrus hystrix DC. showed IC50 values less/equivalent to the positive controls. Weak correlations were observed between the 1,1-diphenyl-2-picryl hydrazyl (DPPH) and xanthine oxidase (XO) assays as well as between the DPPH and nitric oxide radical scavenging (NO) assay and between the XO and 2,2 azinobis (3-ethylbenzothiazoline-6 sulphonic acid (ABTS) assay. Cupressus macrocarpa Hartw., L. nobilis, Cinnamomum zeylanicum Nees, and Psidium guajava L. successfully inhibited in vitro glycated end-products (IC50: 451.53 ± 3.00, 387.04 ± 1.53, 348.59 ± 3.34 and 401.48 ± 2.86 µg/mL respectively) compared to aminoguanidine (IC50: 546.69 ± 3.57 µg/mL). Some of the EOs had a high content of phenolic compounds. EOs such as P. dioica, P. terebinthina, L. nobilis, P. guajava, and C. hystrix were found to be rich in eugenol and other phenolic compounds. The EOs evaluated in the present study may have applications in the nutraceutical and pharmaceutical industries.

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Suggestions on the Contribution of Methyl Eugenol and Eugenol to Bay Laurel (Laurus nobilis L.) Essential Oil Preservative Activity through Radical Scavenging.

Autores: Nikolaos Nenadis, Maria Papapostolou, Maria Z Tsimidou | Ano: 2021 | Database: pubmed

Resumo: The present study examined the radical scavenging potential of the two benzene derivatives found in the bay laurel essential oil (EO), namely methyl eugenol (MEug) and eugenol (Eug), theoretically and experimentally to make suggestions on their contribution to the EO preservative activity through such a mechanism. Calculation of appropriate molecular indices widely used to characterize chain-breaking antioxidants was carried out in the gas and liquid phases (n-hexane, n-octanol, methanol, water). Experimental evidence was based on the DPPH• scavenging assay applied to pure compounds and a set of bay laurel EOs chemically characterized with GC-MS/FID. Theoretical calculations suggested that the preservative properties of both compounds could be exerted through a radical scavenging mechanism via hydrogen atom donation. Eug was predicted to be of superior efficiency in line with experimental findings. Pearson correlation and partial least square regression analyses of the EO antioxidant activity values vs. % composition of individual volatiles indicated the positive contribution of both compounds to the radical scavenging activity of bay laurel EOs. Eug, despite its low content in bay laurel EOs, was found to influence the most the radical scavenging activity of the latter.

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Antioxidant and Anti-Atherogenic Activities of Essential Oils from Myrtus communis L. and Laurus nobilis L. in Rat.

Autores: Dyana Odeh, Nada Oršolić, Marija Berendika, Domagoj Đikić, Sandra Domjanić Drozdek, Sandra Balbino, Maja Repajić, Verica Dragović-Uzelac, Irena Landeka Jurčević | Ano: 2022 | Database: pubmed

Resumo: Essential oils (EOs) from aromatic and medicinal plants, such as myrtle (Myrtus communis L.) and Laurel (Laurus nobilis L.), are gaining popularity as a potential ingredient in functional foods and nutraceuticals. This study aims to investigate whether the essential oils (EOs) could be effective in weight control, antioxidative and antilipidemic status of rats by affecting microbiota and its enzymes activity and whether changes in intestinal enzyme activity affect the health of rats. The intragastric application of laurel and myrtle EOs to rats for two weeks affects weight loss, reduces glycolytic activity, lipid parameters (cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C)) and atherogenic indicators, leading to cardiovascular protection. Laurel EO can be an excellent candidate for the treatment of drug-induced obesity and related diseases, since it affects lipid metabolism in the liver and inhibits the enzymes responsible for the metabolism of carbohydrates into glucose in the digestive tract, leading to weight loss. In contrast, myrtle EO shows a better antioxidant capacity in most tissues, except kidneys, where it causes a pro-oxidative effect, compared to laurel EO. Myrtle EO increases the permeability and instability of the erythrocyte membrane, resulting in a loss of selectivity for the entry of toxic substances into the cell. On the other hand, myrtle EO leads to intestinal inflammation by reducing the number of probiotic bacteria and increasing Enterobacter.

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The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal.

Autores: A Ferreira, C Proença, M L M Serralheiro, M E M Araújo | Ano: 2006 | Database: pubmed

Resumo: Essential oil, ethanolic extract and decoction of 10 plant species from interior Portugal were analyzed for their activity towards acetylcholinesterase (AChE) enzyme and their antioxidant activity. Of these, Melissa officinalis, Paronychia argentea, Sanguisorba minor, Hypericum undulatum and Malva silvestris are used in herbal medicine, Laurus nobilis and Mentha suaveolens as condiments, and Salvia officinalis, Lavandula angustifolia and Lavandula pedunculata also as aromatics. Melissa officinalis and Mentha suaveolens showed AChE inhibitory capacity higher then 50% in the essential oil fraction. Laurus nobilis, Hypericum undulatum, and Sanguisorba minor showed a high inhibition value of AChE in the ethanolic fraction, 64% (1 mg ml(-1)) 68% (0.5 mg ml(-1)), and 78% (1 mg ml(-1)), respectively. Higher values of AChE inhibitory activity were found using decoctions of Lavandula pedunculata, Mentha suaveolens and Hypericum undulatum, 68, 69 and 82% (at a concentration of 5mg dry plant ml(-1) of assay), respectively. The free radical scavenger activity was higher for the polar extracts. In the water extracts most of the plants showed values around 90%. When antioxidant activity was measured with the beta-carotene-linoleic acid assay high activity (65-95%) was also found in the water extracts. Hypericum undulatum, Melissa officinalis and Laurus nobilis showed both high AChE inhibitory capacity and antioxidant activity.

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Comparison of chemical composition of the essential oil of Laurus nobilis L. leaves and fruits from different regions of Hatay, Turkey.

Autores: Mustafa Kemal Sangun, Ebru Aydin, Mahir Timur, Hatice Karadeniz, Mahmut Caliskan, Aydin Ozkan | Ano: 2007 | Database: pubmed

Resumo: The essential oils of the leaves and fruits from bay (Laurus nobilis L.) grown in Antakya, Yayladagi and Samandagi were isolated by solvent extraction and analysed by capillary gas chromatography (GC), gas chromatography and mass spectrometry (GC/MS). In Antakya, Yayladagi and Samandagi the chemical compositions of the fruits and leaves were similar according to qualitative and quantitative analysis. Although in both fruits and leaves the major component was found to be 1.8-Cineole a concentration of about 50% compared with essential oils. The composition of the essential oil from the leaves has high content of 1.8-Cineole, Sabinene and alpha-Terpinyl acetate, but a low content of a-Pinene, alpha-Phellandrene and trans-/beta-osimen. 1.8-Cineole was found major component of the leaves essential oil collected from Samandagi (59.94%) which is sea coast of region. Interestingly alpha-Pinene, beta-Pinene, alpha-Phellandrene, 1.8-Cineole and trans-beta-osimen were found the major components of fruits of Laurus nobilis L. harvested from Antakya, Yayladagi and Samandagi Trans-beta-osimen was detected as the major component of fruits essential oil collected again from Samandagi (28.35%)

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Composition of essential oils of Laurus nobilis, L. nobilis var. angustifolia and Laurus azorica.

Autores: H Hokwerda, R Bos, D H Tattje, T M Malingre | Ano: 1982 | Database: pubmed

Resumo: The essential oils of Laurus nobilis, L. nobilis var. angustifolia and L. azorica were investigated by means of GLC, IR, PMR, CMR and GC-MS. The composition of the oils of the first two mentioned species was about the same with as main constituent 1,8-cineole, whereas the oil of L. azorica was different with low content of 1,8-cineole (10% vice 40-50%), absence of spathulenol, high content of alpha- and beta-pinene and presence of alpha-copaene, cinnamyl acetate, allo-aromadendrene, beta-eudesmol, beta-caryophyllene oxide and 1 (12), 8 (15)-caryophylladiene-9alpha-ol. About 50 components were tentatively identified.

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Essential oil composition and variability of Laurus nobilis L. growing in Tunisia, comparison and chemometric investigation of different plant organs.

Autores: H Marzouki, A Piras, K Bel Haj Salah, H Medini, T Pivetta, S Bouzid, B Marongiu, D Falconieri | Ano: 2009 | Database: pubmed

Resumo: Stems, leaves, buds and flowers of Laurus nobilis L. growing wild in Tunisia were analysed for their essential oil composition. The essential oil of Laurus nobilis L. gathered from different stations were isolated by hydrodistillation and analysed by GC/MS. The oil yields on a dry weight basis ranged between 0.4% and 1.1%. The major component identified was 1,8-cineole, other predominant components were alpha-terpinyl acetate, methyl eugenol, eugenol and linalool. Although the same compounds were present in all plant organs, the leaves differed from the stems in the concentration of 1,8-cineole and methyl eugenol, buds and flowers in the concentration of 1,8-cineole and the stem's oil composition differs from the others in content of methyl eugenol. The results obtained from GC/MS analysis of the volatile oils from individual plant organs were submitted to principal component analysis. Chemometric investigations led to differentiation of stems, leaves and buds-flowers with the respect to the content of 1,8-cineole, metyhyl eugenol and alpha-terpynil acetate; flowers and buds were non-differentiated. Finally, the antibacterial activity of the leaves' essential oils has been assayed.

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Gas chromatography/mass spectrometry analysis of Laurus nobilis essential oil composition of northern Cyprus.

Autores: Hasan Yalçin, Mehtap Anik, Murat Aydin Sanda, Ahmet Cakir | Ano: 2007 | Database: pubmed

Resumo: The chemical composition of the essential oil isolated from the leaves of the Laurus nobilis plant (from the Northern Cyprus Mountains) by hydrodistillation was analyzed by gas chromatography-mass spectrometry. Of the 81 compounds representing 98.74% of total oil, monocyclic monoterpenes such as 1,8-cineole (58.59%), alpha-terpinyl acetate (8.82%), and terpinene-4-ol (4.25%) were the main components. Bicyclic monoterpenes such as alpha- and beta- pinene (3.39-3.25%) and sabinene (3.32%) were also identified. The acyclic monoterpenes linalool (0.19%) and myrcenol (0.10%) were present in smaller amounts, and so were the sesquiterpenes. o-Cymene (1.30%) and p-cymene (1.83%) were the main, while cumin aldehyde (0.24%), dimethylstyrene (0.08%), eugenol (0.16%), methyl eugenol (0.05%), and carvacrol (0.05%) were found as minor, aromatic compounds of laurel oil.

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Essential oil composition of Laurus nobilis L. of different growth stages growing in Iran.

Autores: Mohammadreza Verdian-rizi, Abbas Hadjiakhoondi | Ano: 2008 | Database: pubmed

Resumo: The chemical variations of the essential oil from the aerial parts of Laurus nobilis L. (Lauraceae) have been studied. Plant material has been harvested at each phenological status (vegetative, before anthesis, full flowering and seed-bearing). The oils were obtained by hydrodistillation of the air-dried samples. Analysis by GC and GC-MS of the essential oils has allowed to identify 39 compounds. The main compounds were 1,8-cineole, trans-sabinene hydrate, alpha-terpinyl acetate, methyl eugenol, sabinene, eugenol and alpha-pinene.

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Essential oil composition and variability of Laurus nobilis L. growing in Tunisia, comparison and chemometric investigation of different plant organs.

Autores: H Marzouki, A Piras, K Bel Haj Salah, H Medini, T Pivetta, S Bouzid, B Marongiu, D Falconieri | Ano: 2009 | Database: pubmed

Resumo: Stems, leaves, buds and flowers of Laurus nobilis L. growing wild in Tunisia were analysed for their essential oil composition. The essential oil of Laurus nobilis L. gathered from different stations were isolated by hydrodistillation and analysed by GC/MS. The oil yields on a dry weight basis ranged between 0.4% and 1.1%. The major component identified was 1,8-cineole, other predominant components were alpha-terpinyl acetate, methyl eugenol, eugenol and linalool. Although the same compounds were present in all plant organs, the leaves differed from the stems in the concentration of 1,8-cineole and methyl eugenol, buds and flowers in the concentration of 1,8-cineole and the stem's oil composition differs from the others in content of methyl eugenol. The results obtained from GC/MS analysis of the volatile oils from individual plant organs were submitted to principal component analysis. Chemometric investigations led to differentiation of stems, leaves and buds-flowers with the respect to the content of 1,8-cineole, metyhyl eugenol and alpha-terpynil acetate; flowers and buds were non-differentiated. Finally, the antibacterial activity of the leaves' essential oils has been assayed.

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Comparison of chemical composition of the essential oil of Laurus nobilis L. leaves and fruits from different regions of Hatay, Turkey.

Autores: Mustafa Kemal Sangun, Ebru Aydin, Mahir Timur, Hatice Karadeniz, Mahmut Caliskan, Aydin Ozkan | Ano: 2007 | Database: pubmed

Resumo: The essential oils of the leaves and fruits from bay (Laurus nobilis L.) grown in Antakya, Yayladagi and Samandagi were isolated by solvent extraction and analysed by capillary gas chromatography (GC), gas chromatography and mass spectrometry (GC/MS). In Antakya, Yayladagi and Samandagi the chemical compositions of the fruits and leaves were similar according to qualitative and quantitative analysis. Although in both fruits and leaves the major component was found to be 1.8-Cineole a concentration of about 50% compared with essential oils. The composition of the essential oil from the leaves has high content of 1.8-Cineole, Sabinene and alpha-Terpinyl acetate, but a low content of a-Pinene, alpha-Phellandrene and trans-/beta-osimen. 1.8-Cineole was found major component of the leaves essential oil collected from Samandagi (59.94%) which is sea coast of region. Interestingly alpha-Pinene, beta-Pinene, alpha-Phellandrene, 1.8-Cineole and trans-beta-osimen were found the major components of fruits of Laurus nobilis L. harvested from Antakya, Yayladagi and Samandagi Trans-beta-osimen was detected as the major component of fruits essential oil collected again from Samandagi (28.35%)

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Composition of essential oils of Laurus nobilis, L. nobilis var. angustifolia and Laurus azorica.

Autores: H Hokwerda, R Bos, D H Tattje, T M Malingre | Ano: 1982 | Database: pubmed

Resumo: The essential oils of Laurus nobilis, L. nobilis var. angustifolia and L. azorica were investigated by means of GLC, IR, PMR, CMR and GC-MS. The composition of the oils of the first two mentioned species was about the same with as main constituent 1,8-cineole, whereas the oil of L. azorica was different with low content of 1,8-cineole (10% vice 40-50%), absence of spathulenol, high content of alpha- and beta-pinene and presence of alpha-copaene, cinnamyl acetate, allo-aromadendrene, beta-eudesmol, beta-caryophyllene oxide and 1 (12), 8 (15)-caryophylladiene-9alpha-ol. About 50 components were tentatively identified.

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Effect of guar gum coatings containing essential oils on shelf life and nutritional quality of green-unripe mangoes during low temperature storage.

Autores: Ayeza Naeem, Tanveer Abbas, Tahira Mohsin Ali, Abid Hasnain | Ano: 2018 | Database: pubmed

Resumo: This study investigated the effect of treatment of guar gum coating coupled with essential oils. Harvested unripe green mangoes (UGM) were preserved using edible coatings containing essential oils of Nigella sativa, Coriandrum sativum, Foeniculum vulgare and Laurus nobilis derived using two different solvents (methanol and ethanol) and stored at refrigeration temperature (10°C, 80-85% relative humidity). Physiological and biochemical parameters that assess the quality of fruits were determined. Microbiological analysis was also performed at the start and end of shelf life. Generally, it was observed that ethanolic essential oils supplemented coatings conferred a greater retention of fruit quality as compared to both controls. Bacterial counts were significantly reduced in fruits that were coated with ethanolic essential oil edible coatings. Secondly, the coatings supplemented with ethanolic and methanolic essential oils extended shelf life up to 24days whereas treated and untreated control decayed after 10 and 6days respectively (P<0.05). These results suggested the application of these edible coatings for preservation of unripe green mangoes during cold storage.

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Biological control of Citrus brown spot pathogen, "Alternaria alternata" by different essential oils.

Autores: Taycir Grati Affes, Synda Chenenaoui, Hassen Zemni, Majdi Hammami, Sarra Bachkouel, Wissem Aidi Wannes, Bouzid Nasraoui, Moufida Saidani Tounsi, Salma Lasram | Ano: 2022 | Database: pubmed

Resumo: The antifungal effects of laurel, myrtle and peppermint essential oils and their combinations were investigated in vitro on two strains of Alternaria alternata mycelial growth and in vivo on detached Citrus leaf disease incidence. Myrtle essential oil was rich in α-pinene and 1,8-cineole while peppermint essential oil in menthol and menthone. 1,8-Cineole was the main component of the essential oils from laurel, laurel + myrtle and peppermint + laurel. The combined peppermint and myrtle essential oil was characterized by the predominance of menthol and 1,8-cineole. All tested essential oils, incorporated in potato-dextrose agar, inhibited A. alternata mycelial growth and had a fungistatic effect at concentration 3 mg/mL of medium. A great synergism was detected between peppermint and laurel essential oils against the two strains of A. alternata. The combined laurel and peppermint essential oil reduced mycelial growth rates of inoculated detached leaves at concentration above 1.5 mg/mL.

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Complete essential oils of Laurus nobilisinducing antinociceptiveaction by opioid mechanism in C-Reflex and spinal Wind-Up model in rat/ Aceites esenciales totales de Laurus nobilis inducen acción antinociceptivamediante mecanismo opioide en el modelode Reflejo-C y Wind-Up espinal en ratas

Autores: Valdés, Carlos; Laurido, Claudio; Morales, Bernardo; Jaimes, Luisauris; Vinet, Raúl; Martínez, José L | Ano: 2020 | Database: LILACS

Resumo: The essential oil of Laurus nobilis L. was used to test their antinociceptive efficacy. It was applied intraperitoneally (i.p.) to rats subjected to a nociception test (C reflex and spinal wind-up). The results showed that the essential oil applied at higher doses (0.06 mg/Kg) causes a complete abolition of the spinal wind-up, while the C reflex was unchanged, indicating a clear antinociceptive effect. At lower concentrations (0.012 mg/Kg), there was a lowering in the wind-up by 85% within ten minutes of the essential i.p. oil application. Interestingly, there was an effect of naloxone (0.08 mg/Kg i.p.) When applied, a change occurs that almost entirely reversed the antinociception caused by the essential oil from Laurus nobilis. We conclude that there is a significant antinociceptive effect of the essential oil of Laurus nobilis subjected to electric nociception. In addition, it was observed that naloxone reversed the antinociceptive effect (wind-up) produced by Laurus nobilis.

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Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria.

Autores: Gulsun Akdemir Evrendilek | Ano: 2015 | Database: pubmed

Resumo: In this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (Pimpinella anisum), bay leaves (Laurus nobilis), cinnamon bark (Cinnamomum verum), clove (Eugenia caryophyllata), fennel (Foeniculum vulgare), hop (Humulus lupulus), Istanbul oregano (Origanum vulgare subsp. hirtum), Izmir oregano (Origanum onites), mint (Mentha piperita), myrtus (Myrtus communis), orange peel (Citrus sinensis), sage (Salvia officinalis), thyme (Thymbra spicata), and Turkish oregano (Origanum minutiflorum)--were related to inhibition of 10 bacteria through multiple linear or non-linear (M(N)LR) models-four Gram-positive bacteria of Listeria innocua, coagulase-negative staphylococci, Staphylococcus aureus, and Bacillus subtilis, and six Gram-negative bacteria of Yersinia enterocolitica, Salmonella Enteritidis, Salmonella Typhimurium, Proteus mirabilis, Escherichia coli O157:H7, and Klebsiella oxytoca. A total of 65 compounds with different antioxidant capacity, phenolic substance content and antibacterial properties were detected with 14 plant essential oils. The best-fit M(N)LR models indicated that relative to anise essential oil, the essential oils of oreganos, cinnamon, and thyme had consistently high inhibitory effects, while orange peel essential oil had consistently a low inhibitory effect. Regression analysis indicated that beta-bisabolene (Turkish and Istanbul oreganos), and terpinolene (thyme) were found to be the most inhibitory compounds regardless of the bacteria type tested.

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The effect of Laurus nobilis L. essential oil and different packaging systems on the photo-oxidative stability of Chemlal extra-virgin olive oil.

Autores: Aldjia Taoudiat, Djamel Djenane, Zoulikha Ferhat, Giorgia Spigno | Ano: 2018 | Database: pubmed

Resumo: This study was performed to determine the effect of the addition of Laurus nobilis L. essential oil (EO) (at 0.01% v/v) and of the packaging material (brown and transparent glass or PET) on the oxidative stability of Algerian extra-virgin olive oil (EVOO) stored for 90 days at 25 ± 2 °C under continuous exposure to fluorescent light (~ 900 lux). Control and enriched EVOO was analysed after 30, 60 and 90 days for various parameters. Bio-enrichment of EVOO with EO combined with packaging in brown glass enabled maintenance of the highest amounts of chlorophyll and carotenoids after 90 days of accelerated photo-oxidation. The lowest total phenols content was found in EVOO without EO packed in transparent glass. EO enrichment and brown packaging preserved higher levels of antioxidant activity but could not preserve the oxidation indices until the end of the period of light exposition.

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Supercritical carbon dioxide extraction and characterization of Laurus nobilis essential oil.

Autores: Alessandra Caredda, Bruno Marongiu, Silvia Porcedda, Carla Soro | Ano: 2002 | Database: pubmed

Resumo: Supercritical carbon dioxide extraction allowed essential oil of Laurus nobilis to be obtained. Extraction conditions were as follows: pressure, 90 bar; temperature, 50 degrees C; and carbon dioxide flow, Phi = 1.0 kg/h. Waxes were entrapped in the first separator set at 90 bar and -10 degrees C. The oil was recovered in the second separator working at 15 bar and 10 degrees C. The main components were 1,8-cineole (22.8%), linalool (12.5%), alpha-terpinyl acetate (11.4%), and methyleugenol (8.1%). Comparison with the hydrodistilled oil did not reveal any significant difference. Collection of samples at different extraction times during supercritical extraction allowed the change of the oil composition to be monitored. Lighter compounds such as hydrocarbon and oxygenated monoterpenes were extracted in shorter times than the heavier hydrocarbon and oxygenated sesquiterpenes.

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A simple method to obtain essential oils from Salvia triloba L. and Laurus nobilis L. by using microwave-assisted hydrodistillation.

Autores: Müberra Kosar, Zepnep Tunalier, Temel Ozek, Mine Kürcüglu, K Hüsnü Can Baser | Ano: 2005 | Database: pubmed

Resumo: A microwave-assisted hydrodistillation protocol was modified to extract essential oils from leaves of Salvia triloba L. and Laurus nobilis L. The essential oils of these plants are generally obtained by hydrodistillation or steam distillation. The volatile compounds obtained by microwave-assisted hydrodistillation and hydrodistillation methods were analyzed by GC and GC/MS. Both distillation methods and analytical results were compared. 1,8-Cineole (46.8-54.2%) was the main component in the leaf oils of both samples. Although the distillation was accomplished in a shorter time, oil yields and 1,8-cineole contents were slightly higher in the microwave-assisted hydrodistillation compared to usual hydrodistillation. Microwave-assisted hydrodistillation appears to be an effective method for the production of essential oils.

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Extraction and separation of volatile and fixed oils from berries of Laurus nobilis L. by Supercritical CO2.

Autores: Hanen Marzouki, Alessandra Piras, Bruno Marongiu, Antonella Rosa, M Assunta Dessì | Ano: 2008 | Database: pubmed

Resumo: Isolation of volatile and fixed oils from dried berries of Laurus nobilis L. from Tunisia have been obtained by supercritical fractioned extraction with carbon dioxide. Extraction experiments were carried out at a temperature of 40 degrees C and pressures of 90 and 250 bar. The extraction step performed at 90 bar produced a volatile fraction mainly composed of (E)-beta-ocimene (20.9%), 1,8-cineole (8.8%), alpha-pinene (8.0%), beta-longipinene (7.1%), linalool acetate (4.5%), cadinene (4.7%), beta-pinene (4.2%), alpha-terpinyl acetate (3.8%) and alpha-bulnesene (3.5%). The oil yield in this step of the process was 0.9 % by weight charged. The last extraction step at 250 bar produced an odorless liquid fraction, in which a very small percentage of fragrance compounds was found, whereas triacylglycerols were dominant. The yield of this step was 15.0 % by weight. The most represented fatty acids of the whole berry fixed oil were 12:0 (27.6%), 18:1 n-9 (27.1%), 18:2 n-6 (21.4%), and 16:0 (17,1%), with the 18:1 n-9 and 18:2 n-6 unsaturated fatty acids in particular averaging 329 microg/mg of oil.

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Extraction and separation of volatile and fixed oils from berries of Laurus nobilis L. by Supercritical CO2.

Autores: Hanen Marzouki, Alessandra Piras, Bruno Marongiu, Antonella Rosa, M Assunta Dessì | Ano: 2008 | Database: pubmed

Resumo: Isolation of volatile and fixed oils from dried berries of Laurus nobilis L. from Tunisia have been obtained by supercritical fractioned extraction with carbon dioxide. Extraction experiments were carried out at a temperature of 40 degrees C and pressures of 90 and 250 bar. The extraction step performed at 90 bar produced a volatile fraction mainly composed of (E)-beta-ocimene (20.9%), 1,8-cineole (8.8%), alpha-pinene (8.0%), beta-longipinene (7.1%), linalool acetate (4.5%), cadinene (4.7%), beta-pinene (4.2%), alpha-terpinyl acetate (3.8%) and alpha-bulnesene (3.5%). The oil yield in this step of the process was 0.9 % by weight charged. The last extraction step at 250 bar produced an odorless liquid fraction, in which a very small percentage of fragrance compounds was found, whereas triacylglycerols were dominant. The yield of this step was 15.0 % by weight. The most represented fatty acids of the whole berry fixed oil were 12:0 (27.6%), 18:1 n-9 (27.1%), 18:2 n-6 (21.4%), and 16:0 (17,1%), with the 18:1 n-9 and 18:2 n-6 unsaturated fatty acids in particular averaging 329 microg/mg of oil.

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Allergic contact dermatitis from Laurus nobilis oil induced by massage.

Autores: Esra Adişen, Meltem Onder | Ano: 2007 | Database: pubmed

Resumo: Non-occupational contact allergy to Laurus nobilis (laurel) oil is considered rare. Herein, we report a case of allergic contact dermatitis after a massage with a mixture of olive oil and L. nobilis oil. Patch testing showed (+++) reactions to laurel oil and (++) to the mixture of olive oil and laurel oil.

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Bio-Guided Fractionation Driven by In Vitro α-Amylase Inhibition Assays of Essential Oils Bearing Specialized Metabolites with Potential Hypoglycemic Activity.

Autores: Francesca Capetti, Cecilia Cagliero, Arianna Marengo, Carlo Bicchi, Patrizia Rubiolo, Barbara Sgorbini | Ano: 2020 | Database: pubmed

Resumo: Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by unpaired blood glycaemia maintenance. T2DM can be treated by inhibiting carbohydrate hydrolyzing enzymes (α-amylases and α-glucosidases) to decrease postprandial hyperglycemia. Acarbose and voglibose are inhibitors used in clinical practice. However, these drugs are associated with unpleasant gastrointestinal side effects. This study explores new α-amylase inhibitors deriving from plant volatile specialized metabolites. Sixty-two essential oils (EOs) from different plant species and botanical families were subjected to α-amylase in vitro enzymatic assay and chemically characterized using gas chromatography coupled to mass spectrometry. Several EOs were found to be potential α-amylase inhibitors, and Eucalyptus radiata, Laurus nobilis, and Myristicafragrans EOs displayed inhibitory capacities comparable to that of the positive control (i.e., acarbose). A bio-guided fractionation approach was adopted to isolate and identify the active fractions/compounds of Eucalyptus radiata and Myristica fragrans EOs. The bio-guided fractionation revealed that EOs α-amylase inhibitory activity is often the result of antagonist, additive, or synergistic interactions among their bioactive constituents and led to the identification of 1,8-cineole, 4-terpineol, α-terpineol, α-pinene, and β-pinene as bioactive compounds, also confirmed when they were tested singularly. These results demonstrate that EO oils are a promising source of potential α-amylase inhibitors.

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The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal.

Autores: A Ferreira, C Proença, M L M Serralheiro, M E M Araújo | Ano: 2006 | Database: pubmed

Resumo: Essential oil, ethanolic extract and decoction of 10 plant species from interior Portugal were analyzed for their activity towards acetylcholinesterase (AChE) enzyme and their antioxidant activity. Of these, Melissa officinalis, Paronychia argentea, Sanguisorba minor, Hypericum undulatum and Malva silvestris are used in herbal medicine, Laurus nobilis and Mentha suaveolens as condiments, and Salvia officinalis, Lavandula angustifolia and Lavandula pedunculata also as aromatics. Melissa officinalis and Mentha suaveolens showed AChE inhibitory capacity higher then 50% in the essential oil fraction. Laurus nobilis, Hypericum undulatum, and Sanguisorba minor showed a high inhibition value of AChE in the ethanolic fraction, 64% (1 mg ml(-1)) 68% (0.5 mg ml(-1)), and 78% (1 mg ml(-1)), respectively. Higher values of AChE inhibitory activity were found using decoctions of Lavandula pedunculata, Mentha suaveolens and Hypericum undulatum, 68, 69 and 82% (at a concentration of 5mg dry plant ml(-1) of assay), respectively. The free radical scavenger activity was higher for the polar extracts. In the water extracts most of the plants showed values around 90%. When antioxidant activity was measured with the beta-carotene-linoleic acid assay high activity (65-95%) was also found in the water extracts. Hypericum undulatum, Melissa officinalis and Laurus nobilis showed both high AChE inhibitory capacity and antioxidant activity.

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Bio-Guided Fractionation Driven by In Vitro α-Amylase Inhibition Assays of Essential Oils Bearing Specialized Metabolites with Potential Hypoglycemic Activity.

Autores: Francesca Capetti, Cecilia Cagliero, Arianna Marengo, Carlo Bicchi, Patrizia Rubiolo, Barbara Sgorbini | Ano: 2020 | Database: pubmed

Resumo: Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by unpaired blood glycaemia maintenance. T2DM can be treated by inhibiting carbohydrate hydrolyzing enzymes (α-amylases and α-glucosidases) to decrease postprandial hyperglycemia. Acarbose and voglibose are inhibitors used in clinical practice. However, these drugs are associated with unpleasant gastrointestinal side effects. This study explores new α-amylase inhibitors deriving from plant volatile specialized metabolites. Sixty-two essential oils (EOs) from different plant species and botanical families were subjected to α-amylase in vitro enzymatic assay and chemically characterized using gas chromatography coupled to mass spectrometry. Several EOs were found to be potential α-amylase inhibitors, and Eucalyptus radiata, Laurus nobilis, and Myristicafragrans EOs displayed inhibitory capacities comparable to that of the positive control (i.e., acarbose). A bio-guided fractionation approach was adopted to isolate and identify the active fractions/compounds of Eucalyptus radiata and Myristica fragrans EOs. The bio-guided fractionation revealed that EOs α-amylase inhibitory activity is often the result of antagonist, additive, or synergistic interactions among their bioactive constituents and led to the identification of 1,8-cineole, 4-terpineol, α-terpineol, α-pinene, and β-pinene as bioactive compounds, also confirmed when they were tested singularly. These results demonstrate that EO oils are a promising source of potential α-amylase inhibitors.

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Molecular size fractions of bay leaf (Laurus nobilis) exhibit differentiated regulation of colorectal cancer cell growth in vitro.

Autores: Louise Bennett, Mahinda Abeywardena, Sharon Burnard, Santina Forsyth, Richard Head, Kerryn King, Glen Patten, Peter Watkins, Roderick Williams, Dimitrios Zabaras, Trevor Lockett | Ano: 2013 | Database: pubmed

Resumo: Numerous in vitro studies using solvent or aqueous extracts of raw dietary plant material have demonstrated modulation of colon cancer cell growth and apoptosis and effects on immune and nonimmune pathways of inflammation. We have developed a generic, 3-staged food-compatible process involving heating for conversion of dietary plants into food ingredients and report results on potential colon cancer-regulating properties of processed forms of Bay leaf (Laurus nobilis). In vitro studies demonstrated inhibition of cancer cell growth by processed Bay leaf products in HT-29, HCT-116, Caco-2, and SW-480 human cancer cell lines, which were accompanied by variable levels of elevated apoptosis. Bay leaf also exerted moderate inhibition of cycloxygenase 2 and 5 lipoxygenase enzymatic activity. In addition, these extracts significantly downregulated interferon-γ production in T helper Type 1-stimulated whole blood from healthy donors. Furthermore, size fractionation of the extracts revealed that antiproliferative and proapoptotic activities were associated with low mass (primarily polyphenolics and essential oils) and high mass (primarily proteins including polyphenol oxidase) chemical classes, respectively. Bay leaf exerted in vitro bioactivity that might be relevant to protecting against early events in sporadic colorectal cancer, with potential for further optimization of bioactivity by size-based fractionation.

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Laurel essential oil: biological activities and application for semolina preservation against the red flour beetle Tribolium castaneum (Tenebrionidae).

Autores: Soumaya Haouel-Hamdi, Abir Soltani, Mohamed Ben Hamedou, Olfa Bachrouch, Maha Ben Abada, Chokri Messaoud, Mohamed Ali El Annabi, Jazia Sriti Eljazi, Emna Boushih, Majdi Hammami, Ferid Limam, Jouda Mediouni Ben Jemâa | Ano: 2022 | Database: pubmed

Resumo: The phytochemical composition of Laurus nobilis essential oil and their anticholinesterase, antioxidant, and insecticidal potential were studied. Also, the oil volatile fraction was compared in semolina at the beginning and after storage periods. For that, a headspace solid-phase-microextraction analysis (HS-SPME) coupled with gas chromatography and mass spectrometry was undertaken. Significant quantitative and qualitative differences of the oil volatile fraction were detected according to storage periods and occupation space ratios. Additionally, anti-acetylcholinesterase activity of L. nobilis oil against T. castaneum adults was evaluated. Results revealed that insecticide activity varied according to storage duration and occupation space. Besides, the oil acts on acetylcholine hydrolysis by inhibiting the activity of acetylcholinesterase. These results highlighted that L. nobilis essential oil may be recommended as an eco-friendly alternative for preserving semolina during storage.

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Evaluation of essential oils as an ecological alternative in the search for control Rhipicephalus microplus (Acari: Ixodidae).

Autores: Rafaelle Vinturelle, Camila Mattos, Jéssica Meloni, Helen D Lamberti, Jeane Nogueira, Itabajara da Silva Vaz Júnior, Leandro Rocha, Viviane Lione, Evelize Folly | Ano: 2021 | Database: pubmed

Resumo: The cattle tick Rhipicephalus microplus is a significant problem for livestock, causing losses of billions of dollars per year. This work aimed to determine the chemical composition of essential oils obtained from Laurus nobilis and Copaifera officinalis and evaluate activity against engorged R. microplus females. Chemical composition analyzed by GC-MS revealed the presence of 39 components accounting for 95.38% of the oil in L. nobilis, the most abundant being 1,8-cineol (25.7%), trans-sabinene-hydrate (20.8%), and α-terpinil acetate (15.0%). Chemical analysis of C. officinalis oil identified 25 components corresponding to 80.5% of the total constituents, where the major compounds were β-caryophyllene (21.1%), caryophyllene oxide (10.7%), and α-trans-bergamotene (9.3%). Adult immersion test (AIT) showed that L. nobilis essential oil at 5% or 10% caused 80.5% mortality of engorged females after 24 h and reached 96.9% and 100% mortality on the third day after treatment, respectively. While the essential oil from C. officinalis caused 84.7% mortality after six days at 10% and at 5%, achieved approximately 100% mortality rate at the end of the experiment (day 15). Both essential oils and the combination significantly inhibited egg-laying; however, the combination treatment showed higher effectiveness than the isolated oils at 2.5%. A possible synergic action of L. nobilis and C. officinalis against the cattle tick R. microplus is therefore suggested. The present work introduces a potential alternative for the development of a formulation environment-friendly (green pesticide) used to control cattle tick infestations.

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Diagnostic Potential of FT-IR Fingerprinting in Botanical Origin Evaluation of Laurus nobilis L. Essential Oil is Supported by GC-FID-MS Data.

Autores: Stella A Ordoudi, Maria Papapostolou, Stella Kokkini, Maria Z Tsimidou | Ano: 2020 | Database: pubmed

Resumo: The last years, non-targeted fingerprinting by Fourier-transform infrared (FT-IR) spectroscopy has gained popularity as an alternative to classical gas chromatography (GC)-based methods because it may allow fast, green, non-destructive and cost-effective assessment of quality of essential oils (EOs) from single plant species. As the relevant studies for Laurus nobilis L. (bay laurel) EO are limited, the present one aimed at exploring the diagnostic potential of FT-IR fingerprinting for the identification of its botanical integrity. A reference spectroscopic dataset of 97 bay laurel EOs containing meaningful information about the intra-species variation was developed via principal component analysis (PCA). This dataset was used to train a one-class model via soft independent modelling class analogy (SIMCA). The model was challenged against commercial bay laurel and non-bay laurel EOs of non-traceable production history. Overall, the diagnostic importance of spectral bands at 3060, 1380-1360, 1150 and 1138 cm-1 was assessed using GC-FID-MS data. The findings support the introduction of FT-IR as a green analytical technique in the quality control of these often mislabeled and/or adulterated precious products. Continuous evaluation of the model performance against newly acquired authentic EOs from all producing regions is needed to ensure validity over time.

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Influence of laurel (Laurus nobilis) essential oil on gut function of Black Sea salmon (Salmo labrax) juveniles.

Autores: Osman Tolga Özel, Ekrem Cem Çankiriligil, Selin Ertürk-Gürkan, Isa Coskun, Mustafa Türe | Ano: 2022 | Database: pubmed

Resumo: The present work investigated the effects of dietary incorporation of laurel (Laurus nobilis) essential oil on the zootechnical performance and digestive physiology of juvenile Black Sea salmon (Salmo labrax). In this trial, 15 fiberglass tanks (39  39 cm square and 33 cm high) were used. Forty-five fish (3.52 ± 0.01 g) were placed randomly per tank. Fish were fed for 90 days with the diet containing 50, 100, 200, or 400 mg kg-1 laurel (Laurus nobilis) essential oils, respectively. The work was performed in the recirculating aquaculture system (RAS) operating with freshwater. Fish were manually fed 3% level of live weight during the experiment period. Final weight (FW), weight gain (WG), feed conversion rate (FCR), and specific growth rate (SGR) were not affected by laurel essential oil supplementation. Dietary laurel essential oil (50 mg kg-1) affected positively the surface area of fish intestinal villus that required for digestion. Both villus height and villus width were affected positively in fish fed with 50 mg laurel essential oil kg-1. While incorporation with 100 mg laurel essential oil kg-1 increased the total α-amylase enzyme, 50 mg laurel kg-1 increased lipase enzyme. Moreover, 50 mg laurel essential oil kg-1 increased lactic acid bacteria (LAB) count in fish. Besides, 50 mg laurel essential oil kg-1 reduced the number of total coliform and E. coli.

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Influence of laurel (Laurus nobilis) essential oil on gut function of Black Sea salmon (Salmo labrax) juveniles.

Autores: Osman Tolga Özel, Ekrem Cem Çankiriligil, Selin Ertürk-Gürkan, Isa Coskun, Mustafa Türe | Ano: 2022 | Database: pubmed

Resumo: The present work investigated the effects of dietary incorporation of laurel (Laurus nobilis) essential oil on the zootechnical performance and digestive physiology of juvenile Black Sea salmon (Salmo labrax). In this trial, 15 fiberglass tanks (39  39 cm square and 33 cm high) were used. Forty-five fish (3.52 ± 0.01 g) were placed randomly per tank. Fish were fed for 90 days with the diet containing 50, 100, 200, or 400 mg kg-1 laurel (Laurus nobilis) essential oils, respectively. The work was performed in the recirculating aquaculture system (RAS) operating with freshwater. Fish were manually fed 3% level of live weight during the experiment period. Final weight (FW), weight gain (WG), feed conversion rate (FCR), and specific growth rate (SGR) were not affected by laurel essential oil supplementation. Dietary laurel essential oil (50 mg kg-1) affected positively the surface area of fish intestinal villus that required for digestion. Both villus height and villus width were affected positively in fish fed with 50 mg laurel essential oil kg-1. While incorporation with 100 mg laurel essential oil kg-1 increased the total α-amylase enzyme, 50 mg laurel kg-1 increased lipase enzyme. Moreover, 50 mg laurel essential oil kg-1 increased lactic acid bacteria (LAB) count in fish. Besides, 50 mg laurel essential oil kg-1 reduced the number of total coliform and E. coli.

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Comparison between the conventional method of extraction of essential oil of Laurus nobilis L. and a novel method which uses microwaves applied in situ, without resorting to an oven.

Autores: Guido Flamini, Marianna Tebano, Pier Luigi Cioni, Lucia Ceccarini, Andrea Simone Ricci, Iginio Longo | Ano: 2007 | Database: pubmed

Resumo: A novel microwave method has been applied to the hydrothermal extraction of essential oil from plants. An insulated microwave coaxial antenna was introduced inside a 1000 ml glass flask containing dry Laurus nobilis L. leaves and tap water. Microwave power up to 800 W at 2450 MHz was emitted in continuous wave regime (CW) or in pulsed regime (PR) at 8 kW peak power. Stirring with a magnetic bar and a Clevenger refrigerator connected to the flask enabled to complete the extraction in 1 h. The results of the in situ microwave extraction were compared with those obtained by heating the same reactor with a conventional electric mantle by gas chromatography-mass spectrometry (GC-MS) analysis. Differences were observed both in the composition of the essential oil and from the energetic point of view. The essential oil obtained with microwave (MW) methods contained substantially higher amounts of oxygenated compounds and lower amounts of monoterpenes than conventional method. The in situ microwave heating is safe and versatile; it presents time and energy saving advantages, and therefore it can be considered useful also for industrial applications.

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A simple method to obtain essential oils from Salvia triloba L. and Laurus nobilis L. by using microwave-assisted hydrodistillation.

Autores: Müberra Kosar, Zepnep Tunalier, Temel Ozek, Mine Kürcüglu, K Hüsnü Can Baser | Ano: 2005 | Database: pubmed

Resumo: A microwave-assisted hydrodistillation protocol was modified to extract essential oils from leaves of Salvia triloba L. and Laurus nobilis L. The essential oils of these plants are generally obtained by hydrodistillation or steam distillation. The volatile compounds obtained by microwave-assisted hydrodistillation and hydrodistillation methods were analyzed by GC and GC/MS. Both distillation methods and analytical results were compared. 1,8-Cineole (46.8-54.2%) was the main component in the leaf oils of both samples. Although the distillation was accomplished in a shorter time, oil yields and 1,8-cineole contents were slightly higher in the microwave-assisted hydrodistillation compared to usual hydrodistillation. Microwave-assisted hydrodistillation appears to be an effective method for the production of essential oils.

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Essential Oil Coating: Mediterranean Culinary Plants as Grain Protectants against Larvae and Adults of Tribolium castaneum and Trogoderma granarium.

Autores: Nikos E Papanikolaou, Nickolas G Kavallieratos, Vassilios Iliopoulos, Epameinondas Evergetis, Anna Skourti, Erifili P Nika, Serkos A Haroutounian | Ano: 2022 | Database: pubmed

Resumo: Postharvest agricultural losses constitute a major food security risk. In contrast, postharvest protection is strongly linked with food safety. The present study aims to develop novel postharvest protection tools through a bioprospecting protocol utilizing edible essential oils (EOs) as grain coatings. For this purpose, six Mediterranean culinary plants were selected for evaluation. The EOs of juniper, Juniperus phoenicea L. (Pinales: Cupressaceae), marjoram, Origanum majorana L. (Lamiales: Lamiaceae), oregano, Origanum vulgare ssp. hirtum (Link) A.Terracc. (Lamiales: Lamiaceae), bay laurel, Laurus nobilis L. (Laurales: Lauraceae) and tarhan, Echinophora tenuifolia ssp. sibthorpiana (Guss.) Tutin (Apiales: Apiaceae) were retrieved through steam distillation, while lemon, Citrus limon (L.) Osbeck (Sapindales: Rutaceae) EO was retrieved through cold press extraction. All EOs were formulated to microemulsions (MEs) and applied uniformly as a coating on wheat against larvae and adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Trogoderma granarium Everts (Coleoptera: Dermestidae). All EO-based MEs have been evaluated for the first time as grain coatings. They caused moderate to high mortality to T. castaneum larvae (67.8-93.3% 14 days post-exposure) and T. granarium adults (70.0-87.8% after 7 days of exposure). Citrus limon, O. majorana and E. tenuifolia ssp. sibthorpiana EO-based MEs were the most efficient against T. castaneum larvae, by exhibiting 93.3%, 91.1% and 90.0% mortality 14 days post-exposure, respectively. Origanum majorana, L. nobilis and J. phoenicea EO-based MEs were the most efficient against T. granarium adults, exhibiting 87.8%, 84.4% and 83.3% mortality after 7 days of exposure, respectively. These results indicate that EO-based ME coating is a potent tool against the tested postharvest pests.

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Inhibition of listeriolysin O and phosphatidylcholine-specific production in Listeria monocytogenes by subinhibitory concentrations of plant essential oils.

Autores: A Smith-Palmer, J Stewart, L Fyfe | Ano: 2002 | Database: pubmed

Resumo: Successful infection by Listeria monocytogenes is dependent upon a range of bacterial extracellular proteins including a cytolysin termed listeriolysin O and phosphatidylcholine-specific phospholipase C. Five plant essential oils--bay, clove, cinnamon, nutmeg and thyme--significantly reduced the production of listeriolysin O by L. monocytogenes. The greatest change was observed after culture with oil of thyme, which reduced haemolysis to 52.1 haemolytic units (HU)/ml compared with 99.8 HU/ml observed with the control. Oil of clove was the only oil that also significantly reduced phosphatidylcholine-specific phospholipase C activity. These changes were observed despite the oils causing no change to the final bacterial concentration or total extracellular protein concentration.

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Comparative investigation of Umbellularia californica and Laurus nobilis leaf essential oils and identification of constituents active against Aedes aegypti.

Autores: Nurhayat Tabanca, Cristina Avonto, Mei Wang, Jon F Parcher, Abbas Ali, Betul Demirci, Vijayasankar Raman, Ikhlas A Khan | Ano: 2013 | Database: pubmed

Resumo: Umbellularia californica (California bay laurel) and Laurus nobilis (Mediterranean bay laurel) leaves may be mistaken or used as a substitute on the market due to their morphological similarity. In this study, a comparison of anatomical and chemical features and biological activity of both plants is presented. L. nobilis essential oil biting deterrent and larvicidal activity were negligible. On the other hand, U. californica leaf oil showed biting deterrent activity against Aedes aegypti . The identified active repellents was thymol, along with (-)-umbellulone, 1,8-cineole, and (-)-α-terpineol. U. californica essential oil also demonstrated good larvicidal activity against 1-day-old Ae. aegypti larvae with a LD50 value of 52.6 ppm. Thymol (LD50 = 17.6 ppm), p-cymene, (-)-umbellulone, and methyleugenol were the primary larvicidal in this oil. Umbellulone was found as the principal compound (37%) of U. californica essential oil, but was not present in L. nobilis essential oil. Umbellulone mosquito activity is here reported for the first time.

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Comparative investigation of Umbellularia californica and Laurus nobilis leaf essential oils and identification of constituents active against Aedes aegypti.

Autores: Nurhayat Tabanca, Cristina Avonto, Mei Wang, Jon F Parcher, Abbas Ali, Betul Demirci, Vijayasankar Raman, Ikhlas A Khan | Ano: 2013 | Database: pubmed

Resumo: Umbellularia californica (California bay laurel) and Laurus nobilis (Mediterranean bay laurel) leaves may be mistaken or used as a substitute on the market due to their morphological similarity. In this study, a comparison of anatomical and chemical features and biological activity of both plants is presented. L. nobilis essential oil biting deterrent and larvicidal activity were negligible. On the other hand, U. californica leaf oil showed biting deterrent activity against Aedes aegypti . The identified active repellents was thymol, along with (-)-umbellulone, 1,8-cineole, and (-)-α-terpineol. U. californica essential oil also demonstrated good larvicidal activity against 1-day-old Ae. aegypti larvae with a LD50 value of 52.6 ppm. Thymol (LD50 = 17.6 ppm), p-cymene, (-)-umbellulone, and methyleugenol were the primary larvicidal in this oil. Umbellulone was found as the principal compound (37%) of U. californica essential oil, but was not present in L. nobilis essential oil. Umbellulone mosquito activity is here reported for the first time.

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Safety and efficacy of a feed additive consisting of an essential oil from the leaves of Laurus nobilis L. (laurel leaf oil) for all animal species (FEFANA asbl).

Autores: , Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Mojca Durjava, Maryline Kouba, Marta López-Alonso, Secundino López Puente, Francesca Marcon, Baltasar Mayo, Alena Pechová, Mariana Petkova, Fernando Ramos, Yolanda Sanz, Roberto Edoardo Villa, Ruud Woutersen, Paul Brantom, Andrew Chesson, Josef Schlatter, Dieter Schrenk, Johannes Westendorf, Paola Manini, Fabiola Pizzo, Birgit Dusemund | Ano: 2023 | Database: pubmed

Resumo: Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of an essential oil from the leaves of Laurus nobilis L. (laurel leaf oil), when used as a sensory additive for all animal species. The additive contains up to 4% methyleugenol. The use of the additive at 2 mg/kg complete feed in dogs and cats was considered of low concern. For other long-living and reproductive animals, the use of the additive at 10 mg/kg was considered of concern. For short-living animals, the Panel had no safety concern when the additive is used at 10 mg/kg complete feed for turkeys for fattening, piglets and other growing Suidae, pigs for fattening, veal calves (milk replacer), cattle for fattening and other growing ruminants, horses and rabbits for meat production, salmonids and other fin fish; and at 8.5 mg/kg for chickens for fattening, other growing poultry and other minor species for fattening. The use of laurel leaf oil up to the highest level in feed which was considered of no concern for target animals was also expected to be of no concern for consumers. The additive should be considered as irritant to skin and eyes and the respiratory tract. Due to the high concentration of methyleugenol (≥ 1%), the additive was classified by the applicant as suspected of causing genetic defects and of causing cancer and should be handled accordingly. The use of the additive under the proposed conditions of use was not expected to pose a risk for the environment. Since the leaves of L. nobilis and their preparations were recognised to flavour food and their function in feed would be the same, no further demonstration of efficacy was considered necessary.

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A Comparative Study of the in Vitro Antimicrobial and Synergistic Effect of Essential Oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with Antimicrobial Drugs: New Approach for Health Promoting Products.

Autores: Ahmed Nafis, Ayoub Kasrati, Chaima Alaoui Jamali, Luísa Custódio, Sara Vitalini, Marcello Iriti, Lahcen Hassani | Ano: 2020 | Database: pubmed

Resumo: Laurus nobilis L. (laurel, Lauraceae) and Prunus armeniaca L. (apricot, Rosaceae) are important industrial crops and display significant biological properties, including antimicrobial activity. In this work, essential oils (EOs) prepared from the leaves of both species from Morocco were evaluated for the first time for possible synergistic in vitro antibacterial and antifungal effects with some conventional antimicrobial drugs, namely fluconazole, ciprofloxacin and vancomycin. Samples were further evaluated for chemical composition by gas chromatography-mass spectrometry (GC-MS). The main volatile compounds detected in L. nobilis were eucalyptol (40.85%), α-terpinyl acetate (12.64%) and methyl eugenol (8.72%), while P. armeniaca was dominated essentially by (Z)-phytol (27.18%), pentacosane (15.11%), nonacosane (8.76%) and benzaldehyde (7.25%). Regarding antimicrobial activity, both EOs inhibited significantly all the microorganisms tested. The EO from L. nobilis had the highest activity, with minimal inhibitory concentrations (MICs) ranging from 1.39 to 22.2 mg/mL for bacteria and between 2.77 and 5.55 mg/mL for yeasts. Conversely, the combination of the studied EOs with ciprofloxacin, vancomycin and fluconazol resulted in a noteworthy decrease in their individual MICs. In fact, of the 32 interactions tested, 23 (71.87%) demonstrated total synergism and 9 (28.12%) a partial synergistic interaction. The EO from L. nobilis exhibited the highest synergistic effect with all the antibiotics used, with fractional inhibitory concentration (FIC) index values in the range of 0.266 to 0.75 for bacteria, and between 0.258 and 0.266 for yeast. The synergistic interaction between the studied EOs and standard antibiotics may constitute promising anti-infective agents useful for treating diseases induced by antibiotic-resistant pathogens.

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