PALMAROSA

In vitro effect of seven essential oils on the reproduction of the cattle tick Rhipicephalus microplus.

Autores: Rafael Pazinato, Andréia Volpato, Matheus D Baldissera, Roberto C V Santos, Dilmar Baretta, Rodrigo A Vaucher, Janice L Giongo, Aline A Boligon, Lenita Moura Stefani, Aleksandro Schafer Da Silva | Ano: 2016 | Database: pubmed

Resumo: The acaricidal effect of seven essential oils was examined in vitro against the cattle tick (Rhipicephalus microplus). Engorged female ticks were manually collected in farms of Southern Brazil and placed into petri dishes (n = 10) in order to test the following oils: juniper (Juniperus communis), palmarosa (Cymbopogon martinii), cedar (Cedrus atlantica), lemon grass (Cymbopogon citratus), ginger (Zingiber officinale), geranium (Pelargonium graveolens) and bergamot (Citrus aurantium var bergamia) at concentrations of 1%, 5%, and 10% each. A control group was used to validate the tests containing Triton X-100 only. Treatment effectiveness was measured considering inhibition of tick oviposition (partial or total), egg's weight, and hatchability. C. martinii, C. citratus and C. atlantica essential oils showed efficacy higher than 99% at all concentrations tested. In addition, J. communis, Z. officinale, P. graveolens, and C. aurantium var bergamia oils showed efficiency ranging from 73% to 95%, depending on the concentration tested, where higher concentrations showed greater efficacy. It was concluded that essential oils can affect tick reproduction in vitro by inhibiting oviposition and hatchability.

Resultados: N/A

Conclusão: N/A

Antifungal and antimycotoxigenic effects of Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii essential oils against Fusarium verticillioides.

Autores: Juliana Cristina Castro, Giseli Cristina Pante, Bruno Martins Centenaro, Rafaela Takako Ribeiro De Almeida, Eduardo Jorge Pilau, Benedito Prado Dias Filho, Simone Aparecida Galerani Mossini, Benício Alves De Abreu Filho, Graciette Matioli, Miguel Machinski Junior | Ano: 2020 | Database: pubmed

Resumo: There is an increasing demand for fungi control in grains, especially toxigenic. Also, there is growing concern on the use of synthetic fungicides; thus alternatives are needed. The aim of this study was to evaluate the antifungal and antimycotoxigenic action of essential oils (EOs) from Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii against Fusarium verticillioides, a spoilage and toxigenic fungus. Essential oils were first chemically characterised by gas chromatography coupled to mass spectrometry, and their antioxidant potential was measured by the DPPH, ABTS and FRAP methods. Minimum inhibitory concentration (MIC) and disc diffusion were used to assess antifungal activity. Scanning electron microscopy was used to evaluate morphological changes in the fungus. Antimycotoxigenic activity of the EOs against the production of fumonisin B1 and B2 by F. verticillioides was evaluated using ultra-high-performance liquid chromatography system. Z. officinale, C. zeylanicum and C. martinii EOs were predominantly composed by zingiberene and geranial; eugenol; and geraniol, respectively. All the EOs had high antioxidant power, especially that from C. zeylanicum. The MICs were 250, 500 and 2,000 µg mL-1 for C. zeylanicum, C. martinii and Z. officinale EOs, respectively. Mycelial reduction of F. verticillioides was observed when EOs were used, and the lowest activity was detected in the Z. officinale EO. Overall, the tested EOs promoted structural damage to the fungal cell wall, decreased conidia size and mycelial reduction. Antimycotoxigenic evaluation of the EOs evidenced a significant reduction (p < .05) in the production of fumonisins B1 and B2 with all the EOs evaluated in the study. These results suggest that especially C. zeylanicum and C. martinii EOs are highly useful for controlling F. verticillioides and fumonisins production.

Resultados: N/A

Conclusão: N/A

Effect of essential oils on Aspergillus spore germination, growth and mycotoxin production: a potential source of botanical food preservative.

Autores: Negero Gemeda, Yimtubezinash Woldeamanuel, Daniel Asrat, Asfaw Debella | Ano: 2014 | Database: pubmed

Resumo: To investigate effect of essential oils on Aspergillus spore germination, growth and mycotoxin production.

Resultados: Cymbopogon martinii, Foeniculum vulgare and Trachyspermum ammi (T. ammi) essential oils were tested against toxicogenic isolates of Aspergillus species. T. ammi oil showed highest antifungal activity. Absolute mycelial inhibition was recorded at 1 µl/mL by essential oils of T. ammi. The oil also showed, complete inhibition of spore germination at a concentration of 2 µl/mL. In addition, T. ammi oil showed significant antiaflatoxigenic potency by totally inhibiting aflatoxin production from Aspergillus niger and Aspergillus flavus at 0.5 and 0.75 µl/mL, respectively. Cymbopogon martinii, Foeniculum vulgare and T. ammi oils as antifungal were found superior over synthetic preservative. Moreover, a concentration of 5 336.297 µl/kg body weight was recorded for LC50 on mice indicating the low mammalian toxicity and strengthening its traditional reputations.

Conclusão: N/A

Antifungal and antimycotoxigenic effects of Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii essential oils against Fusarium verticillioides.

Autores: Juliana Cristina Castro, Giseli Cristina Pante, Bruno Martins Centenaro, Rafaela Takako Ribeiro De Almeida, Eduardo Jorge Pilau, Benedito Prado Dias Filho, Simone Aparecida Galerani Mossini, Benício Alves De Abreu Filho, Graciette Matioli, Miguel Machinski Junior | Ano: 2020 | Database: pubmed

Resumo: There is an increasing demand for fungi control in grains, especially toxigenic. Also, there is growing concern on the use of synthetic fungicides; thus alternatives are needed. The aim of this study was to evaluate the antifungal and antimycotoxigenic action of essential oils (EOs) from Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii against Fusarium verticillioides, a spoilage and toxigenic fungus. Essential oils were first chemically characterised by gas chromatography coupled to mass spectrometry, and their antioxidant potential was measured by the DPPH, ABTS and FRAP methods. Minimum inhibitory concentration (MIC) and disc diffusion were used to assess antifungal activity. Scanning electron microscopy was used to evaluate morphological changes in the fungus. Antimycotoxigenic activity of the EOs against the production of fumonisin B1 and B2 by F. verticillioides was evaluated using ultra-high-performance liquid chromatography system. Z. officinale, C. zeylanicum and C. martinii EOs were predominantly composed by zingiberene and geranial; eugenol; and geraniol, respectively. All the EOs had high antioxidant power, especially that from C. zeylanicum. The MICs were 250, 500 and 2,000 µg mL-1 for C. zeylanicum, C. martinii and Z. officinale EOs, respectively. Mycelial reduction of F. verticillioides was observed when EOs were used, and the lowest activity was detected in the Z. officinale EO. Overall, the tested EOs promoted structural damage to the fungal cell wall, decreased conidia size and mycelial reduction. Antimycotoxigenic evaluation of the EOs evidenced a significant reduction (p < .05) in the production of fumonisins B1 and B2 with all the EOs evaluated in the study. These results suggest that especially C. zeylanicum and C. martinii EOs are highly useful for controlling F. verticillioides and fumonisins production.

Resultados: N/A

Conclusão: N/A

Anthelmintic Efficacy of Palmarosa Oil and Curcuma Oil against the Fish Ectoparasite Gyrodactylus kobayashii (monogenean).

Autores: Shun Zhou, Qiuhong Yang, Jing Dong, Yongtao Liu, Ning Xu, Yibin Yang, Xiaohui Ai | Ano: 2022 | Database: pubmed

Resumo: Monogeneans are a serious threat to the development of aquaculture due to the severe economic losses they cause. The prevention and treatment of this disease are increasingly difficult because of the environmental and health concerns caused by the use of chemical anthelmintics and the emergence of drug resistance. It is thus necessary to search for effective alternatives for the treatment of monogenean infections. In the current study, anthelmintic efficacy of 16 selected essential oils (EOs) was investigated using the goldfish (Carassius auratus)-Gyrodactylus kobayashii model. The screening experiment indicated that palmarosa oil and curcuma oil had satisfactory anthelmintic activity against G. kobayashii with EC100 values of 10 and 12 mg/L after 24-h exposure, respectively. The in vivo and in vitro assays indicated anthelmintic efficacy of palmarosa oil against G. kobayashii was in a time and dose-dependent manner. Interestingly, curcuma oil showed an anesthetic effect on G. kobayashii, and its anthelmintic activity was dose-dependent rather than time-dependent in the concentration range tested in this study. Additionally, the 24-h LC50 (50% lethal concentration) against goldfish of these two EOs was 8.19-fold and 5.54-fold higher than their corresponding EC50 (50% effective concentration) against G. kobayashii, respectively. Moreover, exposure to these two EOs at 100% effective concentration against G. kobayashii had no serious physiological and histopathological influence on goldfish. These results demonstrated a high safety for goldfish of these two EOs. Overall, palmarosa oil and curcuma oil could be potential candidates for the treatment of G. kobayashii infections in aquaculture.

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Conclusão: N/A

Bioactivity assessment of essential oils of Cymbopogon species using a network pharmacology approach.

Autores: Hina Bansal, Vusala Sri Sai Pravallika, Gauri Srivastava, Deepak Ganjewala | Ano: 2022 | Database: pubmed

Resumo: Essential oils of Cymbopogon species have wide commercial applications in fragrance, perfumery, and pharmaceuticals as they exhibit a horizon of bioactivities. Here, essential oils of C. flexuosus and C. martinii were analysed to identify bioactive constituents and bioactivities using a network pharmacology approach. Essential oils were isolated using hydro-distillation in a mini Clevenger apparatus. Analysis of essential oils by GC-MS revealed 20 and 15 chemical constituents in C. flexuosus and C. martinii, respectively. An ingredient-target protein-pathway network was constructed comprising 10 oil constituents (citral, geraniol, geranyl acetate, limonene, linalool, α-terpineol, borneol, α-pinene, myrcene, and n-decanol), 14 target proteins, 51 related pathways, and 108 connections. Analyses of the network showed geraniol, geranyl acetate, limonene, linalool, and citral as major active constituents. A core sub-network constructed from the ingredient-target protein-pathway network revealed bioactivities including anti-cancer, anti-inflammatory and neuroprotective. The protein association network pointed out the major target proteins viz., THRB, FXR, ALOX15, and TSHR and pathways like metabolic, and neuroactive ligand-receptor interaction pathways of essential oil constituents. The target proteins and pathways provided insights into the mechanism of action of bioactive constituents. Based on the results of the study, geraniol was correlated with neuroprotective, citral to chemo-preventive, and limonene to anti-inflammatory activities. Thus, the study offers a new way for the assessment of the bioactivities of Cymbopogon species essential oils leading to the development of new biomedicines.

Resultados: N/A

Conclusão: N/A

Atividade antifúngica de óleos essenciais frente a amostras clínicas de Candida albicans isoladas de pacientes HIV positivos/ Antifungal activity of essential oils against clinical samples of Candida albicans isolated from HIV-positive patients

Autores: Almeida, L. F. D; Cavalcanti, Y. W; Castro, R. D; Lima, E. O | Ano: 2012 | Database: LILACS

Resumo: Objetivou-se avaliar a atividade antifúngica dos óleos essenciais de Ocimum basilicum L. (manjericão), Cymbopogon martinii L. (palmarosa), Thymus vulgaris L. (tomilho) e Cinnamomum cassia Blume (canela da china) sobre cepas de Candida albicans isoladas de pacientes HIV positivos e cepa padrão (ATCC 76845). Quinze amostras clínicas de C. albicans (C1-C15) foram repicadas em ágar Sabouraud Dextrose, para confecção de suspensões em solução salina estéril (0,9%) contendo 1,5 x 10(6) UFC mL-1. As emulsões dos óleos essenciais foram preparadas em água destilada estéril e tween 80, com concentrações variando entre 1024 µg mL-1 e 4 µg mL-1. A ação antifúngica foi determinada por meio da Concentração Inibitória Mínima (CIM) utilizando-se a técnica da microdiluição. Foram utilizados como controles positivos a nistatina e o miconazol (50 µg mL-1). Os testes foram realizados em triplicata, sendo a CIM, a menor concentração capaz de inibir o crescimento das leveduras, observada por método visual de acordo com a turvação do meio de cultura. Para C. albicans (ATCC 76845), a CIM do óleo essencial de C. cassia foi 64 µg mL-1, enquanto para óleo de C. martinii foi 1024 µg mL-1. Para as cepas clínicas, verificou-se que a CIM de C. cassia para 80% das cepas foi 64 µg mL-1, sendo a variação dos valores da CIM entre 128 µg mL-1 e 64 µg mL-1. Observou-se que para 66,6% das amostras clínicas, a CIM de C. martinii foi 612 µg mL-1. Constatou-se que a nistatina não apresentou atividade frente às cepas clínicas (C1-C15), enquanto a atividade antifúngica do miconazol foi verificada em 100% das amostras. Não se constatou atividade antimicrobiana dos óleos essenciais de O. basilicum e T. vulgaris, nas concentrações avaliadas. Concluiu-se que os óleos essenciais de C. cassia e C. martinii, em diferentes concentrações, apresentam atividade antifúngica sobre cepas de C. albicans isoladas de pacientes HIV positivos e cepa padrão (ATCC 76845). Entretanto não foi observada inibição antimicrobiana para os óleos de O. basilicum e T. vulgaris.

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Conclusão: N/A

Bio-nanocomposite edible coatings based on arrowroot starch/cellulose nanocrystals/carnauba wax nanoemulsion containing essential oils to preserve quality and improve shelf life of strawberry.

Autores: Josemar Gonçalves de Oliveira Filho, Beatriz Regina Albiero, Ítalo Henrique Calisto, Mirella Romanelli Vicente Bertolo, Fernanda Campos Alencar Oldoni, Mariana Buranelo Egea, Stanislau Bogusz Junior, Henriette Monteiro Cordeiro de Azeredo, Marcos David Ferreira | Ano: 2022 | Database: pubmed

Resumo: This study investigated the effects of bio-nanocomposite coatings developed using arrowroot starch (AA), cellulose nanocrystals (CNC), carnauba wax nanoemulsion (CWN), and Cymbopogon martinii and Mentha spicata essential oils (CEO and MEO, respectively) on the physicochemical, microbiological, bioactive, antioxidant, and aromatic characteristics of strawberries cv. 'Oso Grande' in refrigerated storage for 12 days. The coatings improved the shelf life and stability of strawberries, minimizing their weight loss (2.6-3.9 %), as well as changes in color and texture (except for those coated with CEO), titratable acidity, pH, soluble solids, anthocyanins, phenolic compounds, ascorbic acid content, and antioxidant activity compared with uncoated control strawberries. The bio-nanocomposite coatings containing MEO and CEO also exhibited antimicrobial activity, reduced visible fungal deterioration (40-60 %), and reduced microbial load (3.59-4.03 log CFU g-1 for mesophilic aerobic bacteria and 4.45-5.22 log CFU g-1 for fungi and yeast) during storage. They also significantly reduced the severity of decay caused by inoculation with Botrytis cinerea or Rhizopus stolonifer. The coatings altered the volatile profile of the fruits during storage, decreasing aldehyde and alcohol concentrations and increasing ester concentrations. Thus, these bio-nanocomposite coatings, especially those containing MEO, can be used as antimicrobial coating materials to preserve the post-harvest quality of fresh strawberries.

Resultados: N/A

Conclusão: N/A

Efeito inibitório de óleos essenciais sobre microrganismos do canal radicular/ Inhibitory effect of essential oils against organisms from root canal

Autores: Cavalcanti, Yuri Wanderley; Pérez, Ana Luíza Alves de Lima; Xavier, Gabriela Dantas Rocha; Almeida, Leopoldina de Fátima Dantas de | Ano: 2011 | Database: LILACS

Resumo: Introdução: os óleos essenciais apresentam propriedades biológicas, as quais devem ser estudadas de modo a investigar novas possibilidades de constituição de soluções irrigadoras. Objetivo: avaliar o efeito inibitóriode óleos essenciais extraídos de plantas sobre a atividade de microrganismos envolvidos na infecção do canal radicular. Material e método: foram selecionados os óleos essenciais de Melaleuca alternifolia (melaleuca), Cymbopogon winterianus (citronela), Thymus vulgaris (tomilho), Ocimum basilicum (manjericão), Cymbopogon martinii (palmarosa) e Cinnamomum cassia (canela-da-china). A atividade antimicrobiana sobre Candida albicans (ATCC 289065) e Enterococcus faecalis (ATCC 29212) foi avaliada por meio da triagem da atividade antimicrobiana e da determinação da Concentração Inibitória Mínima (CIM). As soluções de Digluconato de Clorexidina (0,12%) e Hipoclorito de Sódio (1%) serviram de controle. Resultado: os halos de inibição docrescimento para E. faecalis variaram entre 8,00 e 26,00 mm, enquanto que a variação para C. albicans foi entre 26,67 e > 35,00 mm. Os óleos essenciais de T. vulgaris, C. martinii e C. cassia apresentaram CIM na concentração 0,56 mg.mL­1 frente às cepas testadas. Menor atividade foi demonstrada por O. basilicum, que apresentou CIM nas concentrações 72,00 e 1,12 mg.mL­1 contra E. faecalis e C. albicans, respectivamente. Conclusão: os produtos testados apresentaram efeito inibitório satisfatório sobre E. faecalis e C. albicans, destacando-se os óleos essenciais de T. vulgaris, C. martinii e C. cassia.

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Conclusão: N/A

Atividade antimicrobiana de óleos essenciais no controle de alguns fitopatógenos fúngicos in vitro e no tratamento de sementes/ Antimicrobial activity of essential oils on the in vitro control of some fungal phytopathogens and on seed treatment

Autores: Hillen, T; Schwan-Estrada, K. R. F; Mesquini, R. M; Cruz, M. E. S; Stangarlin, J. R; Nozaki, M | Ano: 2012 | Database: LILACS

Resumo: Este trabalho verificou o efeito dos óleos essenciais (OE) extraídos de Eremanthus erythropappus (candeia), Cymbopogon martinii (palmarosa) e de Rosmarinus officinalis (alecrim) no crescimento micelial de alguns fitopatógenos fúngicos e no tratamento de sementes de milho, soja e feijão. No teste in vitro, alíquotas de 20, 40, 60, 100, 200, 500 e 1000 μL de cada um dos óleos essenciais foram distribuídas na superfície do meio de cultura. Posteriormente, discos de meio de cultura com micélio de Alternaria carthami, Alternaria sp. e Rhizoctonia solani foram transferidos para o centro de cada placa. O crescimento foi mensurado e calculada a taxa de inibição do crescimento micelial (ICM). Para verificar o efeito dos OE na germinação das sementes utilizou-se a aplicação deles por fumigação. Foi avaliada a percentagem de sementes germinadas e a incidência de patógenos nas sementes. Sobre o crescimento micelial, o óleo de palmarosa inibiu completamente todos os patógenos fúngicos, independentemente da concentração. Já os óleos de candeia e alecrim foram melhores quando foram adicionadas alíquotas superiores a 200 μL. Os óleos influenciaram diferentemente a germinação e a sanidade das sementes de milho, soja e feijão.

Resultados: N/A

Conclusão: N/A

Investigação das propriedades anticariogênicas de óleos essenciais: atividade antimicrobiana e caracterização química/ Investigation of the anti-cariogenic properties of essential oils: antimicrobial activity, and chemical characterization

Autores: Oliveira, Maria Alcionéia Carvalho de | Ano: 2012 | Database: LILACS

Resumo: O objetivo desse trabalho foi avaliar os óleos essenciais de Thymus vulgaris, Hyptis spicigera, Cymbopogon citratrus, Cymbopogon martinii, Cymbopogon flexuosus bem como os terpenos (citral e cineoli), com relação ao seu potencial para aplicação em terapia anticariogênica. Inicialmente, e a seguir foram realizados testes microbiológicos para a determinação da concentração inibitória mínima (CIM), concentração bactericida mínima (CBM), utilizando os micro-organismos Actinomyces naeslundii ATCC 19039, Lactobacillus acidophilus ATCC 4356, Streptococcus gordonii ATCC 10558, Streptococcus mitis ATCC 9811, Streptococcus mutans ATCC 35688, Streptococcus sanguinis ATCC 10556 e Streptococcus sobrinus ATCC 33478, além de isolados clínicos de estreptococos do grupo mutans e Lactobacillus spp., ambos isolados de dentina cariada. Os três óleos essenciais com maior atividade antibacteriana foram selecionados e caracterizados quimicamente por cromatografia gasosa e avaliados frente ao biofilme de microcosmo, analisando a influência dos óleos essências na adesão de micro-organismo cariogênicos ao esmalte dentário. Além disso, foi analisada a capacidade de eliminação do biofilme de microcosmo sobre a lamínula de vidro. Os resultados revelaram a ação bactericida e bacteriostática de todos os óleos essenciais testados frente às cepas padrão e clínica, com CIMs variando de 0,78 a 3,125μl /mL. Para os terpenos os valores de CIM variaram de 3,125 to 50 μl/mL. No teste de adesão, os resultados apresentam 100% de inibição da adesão do microcosmo em esmalte dentário. Este resultado também foi observado no estudo frente ao biofilme. Diante disso, conclui-se que todos os óleos essenciais e os terpenóides testados possuem atividade promissora frente micro-organismos cariogênicos, além disso, os óleos essenciais de T. vulgaris, H. spicigera e C. citratus, foram capazes de impedir a adesão de micro-organismos bucais ao esmalte dentário

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Conclusão: N/A

Double-bottom antimicrobial packaging for apple shelf-life extension.

Autores: Argus Cezar da Rocha Neto, Randolph Beaudry, Marcelo Maraschin, Robson Marcelo Di Piero, Eva Almenar | Ano: 2019 | Database: pubmed

Resumo: A package was created that extends apple shelf-life by slowing Penicillium expansum growth. The package consisted of a peelable lid and a tray with a double bottom with inclusion complexes (ICs) of ß-cyclodextrin (ß-CD) containing the essential oils of palmarosa (ICp) or of star anise (ICsa). Oil amounts required for antimicrobial activity were obtained from in vitro assays. After 12 days at 23 °C, P. expansum-inoculated apples in both of the double-bottom antimicrobial packages (DBAP) had 1/3 less fungal growth, less than 50% weight loss and ethylene and CO2 production, and less than 25% firmness loss, TA and SSC increase, and pH decrease compared to controls. The DBAP with ICsa performed better than with ICp in reducing ethylene production, respiration rate, firmness loss, TA increase, and pH decrease. This demonstrates DBAP containing ICp or ICsa can maximize the shelf-life of apples injured by P. expansum, validating a novel type of antimicrobial packaging.

Resultados: N/A

Conclusão: N/A

Bio-nanocomposite edible coatings based on arrowroot starch/cellulose nanocrystals/carnauba wax nanoemulsion containing essential oils to preserve quality and improve shelf life of strawberry.

Autores: Josemar Gonçalves de Oliveira Filho, Beatriz Regina Albiero, Ítalo Henrique Calisto, Mirella Romanelli Vicente Bertolo, Fernanda Campos Alencar Oldoni, Mariana Buranelo Egea, Stanislau Bogusz Junior, Henriette Monteiro Cordeiro de Azeredo, Marcos David Ferreira | Ano: 2022 | Database: pubmed

Resumo: This study investigated the effects of bio-nanocomposite coatings developed using arrowroot starch (AA), cellulose nanocrystals (CNC), carnauba wax nanoemulsion (CWN), and Cymbopogon martinii and Mentha spicata essential oils (CEO and MEO, respectively) on the physicochemical, microbiological, bioactive, antioxidant, and aromatic characteristics of strawberries cv. 'Oso Grande' in refrigerated storage for 12 days. The coatings improved the shelf life and stability of strawberries, minimizing their weight loss (2.6-3.9 %), as well as changes in color and texture (except for those coated with CEO), titratable acidity, pH, soluble solids, anthocyanins, phenolic compounds, ascorbic acid content, and antioxidant activity compared with uncoated control strawberries. The bio-nanocomposite coatings containing MEO and CEO also exhibited antimicrobial activity, reduced visible fungal deterioration (40-60 %), and reduced microbial load (3.59-4.03 log CFU g-1 for mesophilic aerobic bacteria and 4.45-5.22 log CFU g-1 for fungi and yeast) during storage. They also significantly reduced the severity of decay caused by inoculation with Botrytis cinerea or Rhizopus stolonifer. The coatings altered the volatile profile of the fruits during storage, decreasing aldehyde and alcohol concentrations and increasing ester concentrations. Thus, these bio-nanocomposite coatings, especially those containing MEO, can be used as antimicrobial coating materials to preserve the post-harvest quality of fresh strawberries.

Resultados: N/A

Conclusão: N/A

Identification of essential oils with activity against stationary phase Staphylococcus aureus.

Autores: Shuzhen Xiao, Peng Cui, Wanliang Shi, Ying Zhang | Ano: 2020 | Database: pubmed

Resumo: Staphylococcus aureus is the most dominant human pathogen, responsible for a variety of chronic and severe infections. There is mounting evidence that persisters are associated with treatment failure and relapse of persistent infections. While some essential oils were reported to have antimicrobial activity against growing S. aureus, activity of essential oils against the stationary phase S. aureus enriched in persisters has not been investigated.

Resultados: We identified 39 essential oils (Oregano, Cinnamon bark, Thyme white, Bandit "Thieves", Lemongrass (Cymbopogon flexuosus), Sandalwood oil, Health shield, Allspice, Amyris, Palmarosa, Cinnamon leaf, Clove bud, Citronella, Geranium bourbon, Marjoram, Peppermint, Lemongrass, Cornmint, Elemi, Ho wood, Head ease, Lemon eucalyptus, Litsea cubeba, Myrrh, Parsley seed, Coriander oil, Dillweed, Hyssop, Neroli, Rosewood oil, Tea tree, Cajeput, Clove bud, Lavender, Sleep tight, Vetiver, Palo santo, Sage oil, Yarrow) at 0.5% (v/v) concentration, 10 essential oils (Cinnamon bark, Oregano, Thyme white, Bandit "Thieves", Lemongrass, Sandalwood oil, Health shield, Allspice, Amyris, Palmarosa at 0.25% (v/v) concentration, and 7 essential oils (Oregano, Cinnamon bark, Thyme white, Lemongrass, Allspice, Amyris, Palmarosa at 0.125% (v/v) concentration to have high activity against stationary phase S. aureus with no visible growth on agar plates after five-day exposure. Among the 10 essential oils which showed high activity at 0.25% (v/v) concentration, 9 (Oregano, Cinnamon bark, Thyme white, Bandit "Thieves", Lemongrass, Health shield, Allspice, Palmarosa, Amyris showed higher activity than the known persister drug tosufloxacin, while Sandalwood oil had activity at a higher concentration. In Oregano essential oil combination studies with antibiotics, Oregano plus tosufloxacin (or levofloxacin, ciprofloxacin) and rifampin completely eradicated stationary phase S. aureus cells, but had no apparent enhancement for linezolid, vancomycin, sulfamethoxazole, trimethoprim, azithromycin or gentamicin.

Conclusão: N/A

Bioactivity assessment of essential oils of Cymbopogon species using a network pharmacology approach.

Autores: Hina Bansal, Vusala Sri Sai Pravallika, Gauri Srivastava, Deepak Ganjewala | Ano: 2022 | Database: pubmed

Resumo: Essential oils of Cymbopogon species have wide commercial applications in fragrance, perfumery, and pharmaceuticals as they exhibit a horizon of bioactivities. Here, essential oils of C. flexuosus and C. martinii were analysed to identify bioactive constituents and bioactivities using a network pharmacology approach. Essential oils were isolated using hydro-distillation in a mini Clevenger apparatus. Analysis of essential oils by GC-MS revealed 20 and 15 chemical constituents in C. flexuosus and C. martinii, respectively. An ingredient-target protein-pathway network was constructed comprising 10 oil constituents (citral, geraniol, geranyl acetate, limonene, linalool, α-terpineol, borneol, α-pinene, myrcene, and n-decanol), 14 target proteins, 51 related pathways, and 108 connections. Analyses of the network showed geraniol, geranyl acetate, limonene, linalool, and citral as major active constituents. A core sub-network constructed from the ingredient-target protein-pathway network revealed bioactivities including anti-cancer, anti-inflammatory and neuroprotective. The protein association network pointed out the major target proteins viz., THRB, FXR, ALOX15, and TSHR and pathways like metabolic, and neuroactive ligand-receptor interaction pathways of essential oil constituents. The target proteins and pathways provided insights into the mechanism of action of bioactive constituents. Based on the results of the study, geraniol was correlated with neuroprotective, citral to chemo-preventive, and limonene to anti-inflammatory activities. Thus, the study offers a new way for the assessment of the bioactivities of Cymbopogon species essential oils leading to the development of new biomedicines.

Resultados: N/A

Conclusão: N/A

Anthelmintic Efficacy of Palmarosa Oil and Curcuma Oil against the Fish Ectoparasite Gyrodactylus kobayashii (monogenean).

Autores: Shun Zhou, Qiuhong Yang, Jing Dong, Yongtao Liu, Ning Xu, Yibin Yang, Xiaohui Ai | Ano: 2022 | Database: pubmed

Resumo: Monogeneans are a serious threat to the development of aquaculture due to the severe economic losses they cause. The prevention and treatment of this disease are increasingly difficult because of the environmental and health concerns caused by the use of chemical anthelmintics and the emergence of drug resistance. It is thus necessary to search for effective alternatives for the treatment of monogenean infections. In the current study, anthelmintic efficacy of 16 selected essential oils (EOs) was investigated using the goldfish (Carassius auratus)-Gyrodactylus kobayashii model. The screening experiment indicated that palmarosa oil and curcuma oil had satisfactory anthelmintic activity against G. kobayashii with EC100 values of 10 and 12 mg/L after 24-h exposure, respectively. The in vivo and in vitro assays indicated anthelmintic efficacy of palmarosa oil against G. kobayashii was in a time and dose-dependent manner. Interestingly, curcuma oil showed an anesthetic effect on G. kobayashii, and its anthelmintic activity was dose-dependent rather than time-dependent in the concentration range tested in this study. Additionally, the 24-h LC50 (50% lethal concentration) against goldfish of these two EOs was 8.19-fold and 5.54-fold higher than their corresponding EC50 (50% effective concentration) against G. kobayashii, respectively. Moreover, exposure to these two EOs at 100% effective concentration against G. kobayashii had no serious physiological and histopathological influence on goldfish. These results demonstrated a high safety for goldfish of these two EOs. Overall, palmarosa oil and curcuma oil could be potential candidates for the treatment of G. kobayashii infections in aquaculture.

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Estudio comparativo de la composición de los aceites esenciales de cuatro especies del género Cymbopogon (Poaceae) cultivadas en Colombia/ Comparative study of the essential oil compositions of four Cymbopogon (Poaceae) species grown in Colombia

Autores: Rodríguez Quintanilla, Raúl; Ruiz Nova, Carlos; Arias Moyano, Ginna; Castro Salazar, Hans; Martínez, Jairo; Stashenko, Elena | Ano: 2012 | Database: LILACS

Resumo: Essential oils of C. citratus, C. nardus, C. flexuosus y C. martinii, cultivated in experimental plots in Bucaramanga, Colombia, were subjected to a comparative study of their compositions. The essential oils were obtained by means of microwave radiation-assisted hydrodistillation from freshly cut leaves. Component identification was performed by GC-MS analysis and their quantification employed GC-FID, with n-tetradecane as internal standard. A calibration curve was employed for each main constituent. The most abundant components were geranial (46,3 percent), neral (32,88 percent) and beta-myrcene (10,5 percent) in C. citratus; citronellal (45,7 percent), geraniol (20,4 percent) and citronelol (9,9 percent) in C. nardus; geranial (50,2 percent) and neral (28,7 percent) in C. flexuosus and geraniol (69,6 percent) and geranyl acetate (15,6 percent) in C. martinii. Extraction yields were 0,46, 1,06, 0,46 and 1,2 percent for C. citratus, C. nardus, C. flexuosus and C. martinii, respectively. The observed relative amounts of the most abundant constituent in C. martinii, C. flexuosus and C. citratus oils were similar to those found in oils of the same species in India.

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Conclusão: N/A

Bioatividade de óleos essenciais no controle de Botrytis cinerea isolado de morangueiro/ Essential oils bioactivity in strawberry grey mould control

Autores: Lorenzetti, E. R; Monteiro, F. P; Souza, P. E; Souza, R. J; Scalice, H. K; Diogo Júnior, R; Pires, M. S. O | Ano: 2011 | Database: LILACS

Resumo: Objetivou-se avaliar o uso de óleos essenciais sobre isolados de Botrytis cinerea, causador do mofo cinzento em morangueiro. Foram testados óleos essenciais de capim-limão, palmarosa, citronela, cravo, canela, menta, lavanda, tangerina, eucalipto, melaleuca, alecrim e laranja, todos estes analisados em cromatógrafo a gás acoplado a detector de massas, para identificação dos principais componentes dos óleos. Foram avaliados o crescimento micelial, produção e germinação de conídios de B. cinerea, com a incorporação do óleo no meio de cultura. Realizou-se ainda uma avaliação de voláteis e a eficiência de óleos em isolado resistente a fungicida. Para cada teste, diferentes óleos apresentaram eficiência, contudo capim limão, palmarosa, canela e menta demonstraram os melhores efeitos em todos os testes realizados. Todos os tratamentos a base de óleos demonstraram efeito semelhante a um fungicida recomendado para a cultura, a base de tiofanato metílico. Dois tratamentos mostraram-se efetivos no caso de isolado resistente (óleo de capim limão e de canela). Óleos essenciais mostram-se como opção promissora para o desenvolvimento de possíveis produtos fitossanitários para o manejo de doenças em plantas.

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Conclusão: N/A

In vitro effect of seven essential oils on the reproduction of the cattle tick Rhipicephalus microplus.

Autores: Rafael Pazinato, Andréia Volpato, Matheus D Baldissera, Roberto C V Santos, Dilmar Baretta, Rodrigo A Vaucher, Janice L Giongo, Aline A Boligon, Lenita Moura Stefani, Aleksandro Schafer Da Silva | Ano: 2016 | Database: pubmed

Resumo: The acaricidal effect of seven essential oils was examined in vitro against the cattle tick (Rhipicephalus microplus). Engorged female ticks were manually collected in farms of Southern Brazil and placed into petri dishes (n = 10) in order to test the following oils: juniper (Juniperus communis), palmarosa (Cymbopogon martinii), cedar (Cedrus atlantica), lemon grass (Cymbopogon citratus), ginger (Zingiber officinale), geranium (Pelargonium graveolens) and bergamot (Citrus aurantium var bergamia) at concentrations of 1%, 5%, and 10% each. A control group was used to validate the tests containing Triton X-100 only. Treatment effectiveness was measured considering inhibition of tick oviposition (partial or total), egg's weight, and hatchability. C. martinii, C. citratus and C. atlantica essential oils showed efficacy higher than 99% at all concentrations tested. In addition, J. communis, Z. officinale, P. graveolens, and C. aurantium var bergamia oils showed efficiency ranging from 73% to 95%, depending on the concentration tested, where higher concentrations showed greater efficacy. It was concluded that essential oils can affect tick reproduction in vitro by inhibiting oviposition and hatchability.

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Conclusão: N/A

Atividade biológica in vitro de própolis e óleos essenciais sobre o fungo Colletotrichum musae isolado de bananeira (Musa spp. )/ In vitro biological activity of propolis and essential oils on the fungus Colletotrichum musae isolated from banana Musa spp

Autores: BARBOSA, M.S.; VIEIRA, G.H.C.; TEIXEIRA, A.V. | Ano: 2015 | Database: LILACS

Resumo: RESUMO: No Brasil existem várias doenças fúngicas que acometem a bananeira. Destas, pode-se citar a antracnose, responsável por grandes prejuízos à cultura, cujo agente causal é o fungo Colletotrichum musae. A principal forma de controle dessa enfermidade é através da aplicação de fungicidas a base de tiabendazol ou tiofanato metílico. Esse manejo, embora eficiente, favorece o desenvolvimento de resistência do patógeno, causa danos ao ambiente e ao produtor, deixando ainda resíduos nos frutos. Esses fatores têm favorecido a busca por substâncias alternativas com capacidade de controlar o fungo e que não sejam nocivas ao ambiente e, principalmente, que sejam seguras ao consumidor final. Dentre as opções, surge o interesse pelo uso de certos óleos essenciais e da própolis, ambos conhecidos por possuírem propriedades fungicidas. O presente trabalho foi desenvolvido com o objetivo de determinar o potencial fungitóxico "in vitro" da própolis e dos óleos essenciais de palmarosa (Cymbopogon martinii), de teatree (Melaleuca alternifolia), de cravo (Eugenia caryophyllata), e de eucalipto (Corymbia citriodora), sobre Colletotrichum musae. O desenvolvimento experimental consistiu em adicionar inóculos fúngicos de 5 mm, obtidos a partir de colônias puras, ao meio de cultura BDA (batata-dextrose-ágar) acrescido das referidas substâncias em diferentes concentrações (0, 25, 50, 75, 100 e 125 µL/L). Paralelo aos tratamentos realizou-se teste com o fungicida padrão para comparações das médias. A eficiência das substâncias sobre o fungo foi determinada através das avaliações do crescimento micelial das colônias (média de duas medidas diametralmente opostas). Os valores de crescimento micelial obtidos foram utilizados também para o cálculo do índice de velocidade de crescimento micelial. O delineamento experimental utilizado foi inteiramente casualizado em esquema fatorial 5 x 6 + 1, (cinco substâncias em seis concentrações + fungicida), com cinco repetições. Os óleos de tea tree, cravo e palmarosa foram eficientes no controle do fungo Colletotrichum musae não diferindo do fungicida a partir da dose de 50 µL/L em todas as avaliações, apresentando potencial para controle em cultivos orgânicos ou em sistemas de manejo integrado.

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Effect of Cymbopogon martinii, Foeniculum vulgare, and Trachyspermum ammi Essential Oils on the Growth and Mycotoxins Production by Aspergillus Species.

Autores: Negero Gemeda, Yimtubezinash Woldeamanuel, Daniel Asrat, Asfaw Debella | Ano: 2014 | Database: pubmed

Resumo: This study was performed to investigate effect of essential oils on Aspergillus spore germination, growth, and mycotoxin production. In vitro antifungal and antiaflatoxigenic activities of Cymbopogon martinii, Foeniculum vulgare, and Trachyspermum ammi essential oils were carried out on toxigenic strains of Aspergillus species. Plant materials were hydrodistilled for 4-5 h in Clevenger apparatus. 0.25 μL/mL, 0.5 μL/mL, 1 μL/mL, 2 μL/mL, and 4 μL/mL concentrations of each essential oil were prepared in 0.1% Tween 80 (V/V). T. ammi oil showed highest antifungal activity. Absolute mycelial inhibition was recorded at 1 μL/mL by essential oils of T. ammi. The oil also showed complete inhibition of spore germination at a concentration of 2 μL/mL. In addition, T. ammi oil showed significant antiaflatoxigenic potency by totally inhibiting toxin production from A. niger and A. flavus at 0.5 and 0.75 μL/mL, respectively. C. martinii, F. vulgare, and T. ammi oils as antifungals were found superior over synthetic preservative. Moreover, a concentration of 5336.297 μL/kg body weight was recorded for LC50 on mice indicating the low mammalian toxicity. In conclusion, the essential oils from T. ammi can be a potential source of safe natural food preservative for food commodities contamination by Aspergillus species.

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Recent advances on the efficacy of essential oils on mycotoxin secretion and their mode of action.

Autores: Adel Mirza Alizadeh, S Amirhossein Golzan, Aida Mahdavi, Samira Dakhili, Zahra Torki, Hedayat Hosseini | Ano: 2022 | Database: pubmed

Resumo: Essential oils, as extracted compounds from plants, are volatile and aromatic liquids which their unique aromatic compounds give each essential oil its distinctive essence. Fungi toxins can induce various adverse health effects like allergy, cancer, and immunosuppression. Moreover, fungal spoilage impacts pharmaceutical and food industries economic state. A drop in the utilization of synthetic compounds as food prophylaxis has occurred due to several factors such as hygiene agents' alerts and stricter legal regulations. Therefore, the applications of natural substances such as essential oils have increased in recent years. Oregano, cinnamon, thyme, rosemary, fennel, clove, palmarosa, and eucalyptus have been the highest employed essential oils against mycotoxigenic fungi and their mycotoxins in studies conducted in the past decade. Essential oils inhibit fungi growth and mycotoxin synthesis via diverse pathways including modified fungal growth rate and extended lag phase, disruption of cell permeability, disruption of the electron transport chain and manipulating gene expression patterns and metabolic processes. In the present review, we will investigate the implications and efficacy of essential oils in preventing the growth of mycotoxigenic fungi, eliminating mycotoxins and their mechanism of actions conducted in the last decade.HighlightsThe most investigated toxigenic genera are Aspergillus, Fusarium and Penicillium Spp.AB1, AG1, OTA and AB2 are the most frequently studied toxinsOregano, cinnamon and thyme are mostly exploited EOs on toxigenic fungi & mycotoxinsOregano, thyme & cinnamon are the most significant antifungals on toxigenic generaCinnamon, oregano & cinnamaldehyde are the fittest antimycotoxins on DON, OTA & AFB1.

Resultados: N/A

Conclusão: N/A

Recent advances on the efficacy of essential oils on mycotoxin secretion and their mode of action.

Autores: Adel Mirza Alizadeh, S Amirhossein Golzan, Aida Mahdavi, Samira Dakhili, Zahra Torki, Hedayat Hosseini | Ano: 2022 | Database: pubmed

Resumo: Essential oils, as extracted compounds from plants, are volatile and aromatic liquids which their unique aromatic compounds give each essential oil its distinctive essence. Fungi toxins can induce various adverse health effects like allergy, cancer, and immunosuppression. Moreover, fungal spoilage impacts pharmaceutical and food industries economic state. A drop in the utilization of synthetic compounds as food prophylaxis has occurred due to several factors such as hygiene agents' alerts and stricter legal regulations. Therefore, the applications of natural substances such as essential oils have increased in recent years. Oregano, cinnamon, thyme, rosemary, fennel, clove, palmarosa, and eucalyptus have been the highest employed essential oils against mycotoxigenic fungi and their mycotoxins in studies conducted in the past decade. Essential oils inhibit fungi growth and mycotoxin synthesis via diverse pathways including modified fungal growth rate and extended lag phase, disruption of cell permeability, disruption of the electron transport chain and manipulating gene expression patterns and metabolic processes. In the present review, we will investigate the implications and efficacy of essential oils in preventing the growth of mycotoxigenic fungi, eliminating mycotoxins and their mechanism of actions conducted in the last decade.HighlightsThe most investigated toxigenic genera are Aspergillus, Fusarium and Penicillium Spp.AB1, AG1, OTA and AB2 are the most frequently studied toxinsOregano, cinnamon and thyme are mostly exploited EOs on toxigenic fungi & mycotoxinsOregano, thyme & cinnamon are the most significant antifungals on toxigenic generaCinnamon, oregano & cinnamaldehyde are the fittest antimycotoxins on DON, OTA & AFB1.

Resultados: N/A

Conclusão: N/A

Effect of essential oils on Aspergillus spore germination, growth and mycotoxin production: a potential source of botanical food preservative.

Autores: Negero Gemeda, Yimtubezinash Woldeamanuel, Daniel Asrat, Asfaw Debella | Ano: 2014 | Database: pubmed

Resumo: To investigate effect of essential oils on Aspergillus spore germination, growth and mycotoxin production.

Resultados: Cymbopogon martinii, Foeniculum vulgare and Trachyspermum ammi (T. ammi) essential oils were tested against toxicogenic isolates of Aspergillus species. T. ammi oil showed highest antifungal activity. Absolute mycelial inhibition was recorded at 1 µl/mL by essential oils of T. ammi. The oil also showed, complete inhibition of spore germination at a concentration of 2 µl/mL. In addition, T. ammi oil showed significant antiaflatoxigenic potency by totally inhibiting aflatoxin production from Aspergillus niger and Aspergillus flavus at 0.5 and 0.75 µl/mL, respectively. Cymbopogon martinii, Foeniculum vulgare and T. ammi oils as antifungal were found superior over synthetic preservative. Moreover, a concentration of 5 336.297 µl/kg body weight was recorded for LC50 on mice indicating the low mammalian toxicity and strengthening its traditional reputations.

Conclusão: N/A

Investigation of the dermal sensitization potential of various essential oils in the local lymph node assay.

Autores: J Lalko, A M Api | Ano: 2006 | Database: pubmed

Resumo: Essential oils are commonly used fragrance ingredients. The oils themselves are complex mixtures, which may contain naturally occurring contact sensitizers. The local lymph node assay was used to evaluate the dermal sensitization potential of basil, citronella, clove leaf, geranium, litsea cubeba, lemongrass, and palmarosa oils. Three of the major components--citral, eugenol, and geraniol--were included to investigate any difference in sensitization potential arising from their exposure in a mixture. Each fragrance material was tested at five concentration ranging from 2.5% to 50% w/v in 1:3 ethanol:diethyl phthalate. The stimulation index (SI) values were calculated for each dose level, an SI > or = 3 was considered a positive response. The estimated concentration (EC3) required to elicit a positive was calculated and taken as a measure of relative potency. The EC3 values and potency classification for basil, clove leaf, litsea cubeba, lemongrass and palmarosa oils were calculated to be <2.5% (> or = moderate), 7.1% (weak), 8.4% (weak), 6.5% (weak) and 9.6% (weak), respectively. Citronella and geranium oils were negative. The individual components citral, eugenol and geraniol resulted in EC3 values of 6.3%, 5.4% and 11.4%, respectively. In general, the potency of each essential oil did not differ significantly from that observed for its main individual component.

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Conclusão: N/A

Investigation of the dermal sensitization potential of various essential oils in the local lymph node assay.

Autores: J Lalko, A M Api | Ano: 2006 | Database: pubmed

Resumo: Essential oils are commonly used fragrance ingredients. The oils themselves are complex mixtures, which may contain naturally occurring contact sensitizers. The local lymph node assay was used to evaluate the dermal sensitization potential of basil, citronella, clove leaf, geranium, litsea cubeba, lemongrass, and palmarosa oils. Three of the major components--citral, eugenol, and geraniol--were included to investigate any difference in sensitization potential arising from their exposure in a mixture. Each fragrance material was tested at five concentration ranging from 2.5% to 50% w/v in 1:3 ethanol:diethyl phthalate. The stimulation index (SI) values were calculated for each dose level, an SI > or = 3 was considered a positive response. The estimated concentration (EC3) required to elicit a positive was calculated and taken as a measure of relative potency. The EC3 values and potency classification for basil, clove leaf, litsea cubeba, lemongrass and palmarosa oils were calculated to be <2.5% (> or = moderate), 7.1% (weak), 8.4% (weak), 6.5% (weak) and 9.6% (weak), respectively. Citronella and geranium oils were negative. The individual components citral, eugenol and geraniol resulted in EC3 values of 6.3%, 5.4% and 11.4%, respectively. In general, the potency of each essential oil did not differ significantly from that observed for its main individual component.

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Conclusão: N/A